CN204132129U - A kind of tissue culture bottle - Google Patents

A kind of tissue culture bottle Download PDF

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Publication number
CN204132129U
CN204132129U CN201420632253.6U CN201420632253U CN204132129U CN 204132129 U CN204132129 U CN 204132129U CN 201420632253 U CN201420632253 U CN 201420632253U CN 204132129 U CN204132129 U CN 204132129U
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CN
China
Prior art keywords
bottle
grid
tissue culture
culture bottle
test
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201420632253.6U
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Chinese (zh)
Inventor
周丽
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
XINGYI NORMAL UNIVERSITY FOR NATIONALITIES
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XINGYI NORMAL UNIVERSITY FOR NATIONALITIES
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Priority to CN201420632253.6U priority Critical patent/CN204132129U/en
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Publication of CN204132129U publication Critical patent/CN204132129U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The utility model relates to a kind of tissue culture bottle, comprise bottle (1) and bottle cap (2), bottle cap (2) material is transparent plastic, bottle (1) material is quartz glass, and described bottle (1) bottom quartz glass is divided into (3) between corresponding grid equably.This tissue culture bottle adds between grid and comes the space that lattice open bottle bottom bottom traditional tissue culture bottle, control clump (strain) number of test-tube plantlet in each tissue culture bottle, ensure that every clump of (strain) test-tube plantlet can independent growths equably, more sturdy test-tube plantlet can be cultivated like this, after seedling, during bottle outlet, operation is very easy, greatly save amount of labour used during bottle outlet, also can not damage root system and cauline leaf simultaneously, can greatly improve bottle outlet efficiency and transplanting survival rate.

Description

A kind of tissue culture bottle
Technical field
The utility model relates to a kind of plant tissue culture bottle, is applied to the field of tissue culture of plant, uses during aseptic fast seedling raising, be specially adapted to the orchid family Dendrobium, Paphiopedilum, Cymbidium or other tissue culture time the well developed root system kind that is easily intertwined.
Background technology
Existing tissue culture bottle is an overall space, during use, 15 ~ 25 seedlings are inoculated in a blake bottle, along with seedling is grown up gradually, the warped roots of test-tube plantlet is bottom blake bottle, deep-rooted root is intricate to combine together, one is formed at bottle bottom dish, during bottle outlet very not easily, very large damage can be caused to the root of test-tube plantlet, stem, leaf, greatly reduce transplanting survival rate, simultaneously due to inconvenient operation when warped roots causes bottle outlet, wash away medium inconvenience after bottle outlet, cause and transplant time-consuming taking a lot of work.
Summary of the invention
The purpose of this utility model is wound around problem for root system of plant in plant tissue culture aseptic culture process to provide tissue culture bottle between a kind of grid, and when final singling is cultivated, every strain seedling grows into enough healthy and strong, can not pull apart root system during bottle outlet.
The utility model is for test-tube plantlet growth provides independent growths space in tissue culture bottle, and respective root system alone becomes one, can not be subject to machinery and pull when test-tube plantlet bottle outlet is transplanted.
The purpose of this utility model and technical problem underlying thereof realize by the following technical solutions:
A kind of tissue culture bottle, comprises bottle and bottle cap, it is characterized in that: the bottom of bottle is shaped between grid, and the quantity between grid determines according to bottle sizes.
Between grid, size is more even, vertical view at the bottom of its bottle: be square 4 between middle grid, between the grid adjacent with bottle edge, outer shape changes with bottle edge radian and changes 5, the bottom side length 1.8 ~ 2.0cm between square lattice, high 2.5 ~ 3cm.
Jar base diameter 6.8 cm of capacity 370ml arranges between 16 grid, the osculum tissue culture bottle of capacity 650ml, and base diameter is 9.6cm, to arrange between 25 grid 7.Bottom side length 1.8 ~ 2.0cm between sub-box, between grid, the end is square, high 2.5 ~ 3cm.
Between grid, size is comparatively even, and be square between middle grid, between the grid adjacent with bottle edge, outer shape changes with bottle edge radian and changes, the bottom side length 1.8 ~ 2.0cm between square lattice, high 2.5 ~ 3cm.
Between grid, sidewall there is aperture to communicate, between the grid having aperture to communicate, claim mutual-through type structure, claim stand-alone configuration without between the grid that aperture communicates.
Between bottle and grid, material is transparent quartz glass, and bottle cap is transparent plastic.Bottleneck diameter is less than bottle diameter, and bottle cap and bottleneck are by screw thread mouth compact siro spinning technology.Be combined as a whole with bottle between grid structure.
After can making the overall structure between bottle bottom and grid with mould during production, then be bonded into overall bottle with bottle middle and upper part.
Aperture between the utility model grid above sidewall makes the material in the medium between grid produce interchange by aperture, has the species of population effect to grow fast when this is conducive to group training.Without aperture absolute construction formula grid between bottle be suitable for without population effect or be easy to the species of taking root.
The utility model in tissue culture bottle for test-tube plantlet growth provides independent growths space, respective root system alone becomes one, the transplanting of test-tube plantlet bottle outlet can not be subject to machinery and pull, substantially increase transplanting survival rate, simultaneously because root system can not be wound around, bottle outlet is easy to operate, is easy to wash away medium, time and labour saving after bottle outlet.
Accompanying drawing explanation
Fig. 1 the utility model structural representation, stand-alone configuration;
Fig. 2 bottle bottom vertical view, right 25 lattice of left 16 lattice;
Fig. 3 the utility model structural representation, mutual-through type structure;
Fig. 4 bottle overall package schematic diagram is example in a free-standing.
In figure: 1 bottle, 2 bottle caps, between 3 grid, between 4 square lattices, between 5 irregular shape grid, 6 tool 16 lattice blake bottle bottom plan view, 7 tool 25 lattice blake bottle bottom plan view, 8 apertures, 9 bottlenecks, overall structure at the bottom of 10 bottles and between grid, 11 bottle middle and upper parts.
Embodiment
Below in conjunction with accompanying drawing, the utility model is described further.
A kind of tissue culture bottle of the utility model, comprises bottle 1 and bottle cap 2, and the bottom of bottle 1 to be shaped between grid 3, and between grid, the quantity of 3 determines according to bottle sizes.
Between grid, size is more even, vertical view at the bottom of its bottle: be square 4 between middle grid, between the grid adjacent with bottle edge, outer shape changes with bottle edge radian and changes 5, the bottom side length 1.8 ~ 2.0cm between square lattice, high 2.5 ~ 3cm.
Jar base diameter 6.9 cm of capacity 370ml arranges the osculum tissue culture bottle of between 16 grid 6, capacity 650ml, and base diameter is 9.6cm, to arrange between 25 grid 7.
Between grid 3 sidewall above aperture 8 can be had to communicate, claim mutual-through type structure between the grid having aperture to communicate, claim stand-alone configuration without between the grid that aperture communicates.
Between bottle 1 and grid, 3 materials are transparent quartz glass, and bottle cap is transparent plastic.
Bottleneck 9 diameter is less than bottle 1 diameter, and bottle cap 2 and bottleneck 9 are by screw thread mouth compact siro spinning technology.
Between grid, 3 to be combined as a whole structure with bottle.
After can making the overall structure 10 between bottle bottom and grid with mould during production, then be bonded into overall bottle with bottle middle and upper part 11.
During use, medium is injected at the bottom of bottle from bottleneck, highly for just to cover hole wall highest point, just can inoculate final singling after covering tightly bottle cap sterilizing and cultivate all kinds of seedling.
According to needs when cultivating after test-tube plantlet bottle outlet, during use, 16 ~ 25 (or clump) seedlings are inoculated between the grid in blake bottle, 1 little Cong seedling of indirect 1 seedling of each grid or tool 2 ~ 3 seedling, seedling is raw in independently grid grows up strong and sturdy, but root system can not be wound around, bottle outlet is very convenient.
The foregoing is only preferred embodiment of the present utility model, all equalizations done according to the utility model claim change and modify, and all belong to the scope that the utility model is contained.

Claims (8)

1. a tissue culture bottle, comprises bottle (1) and bottle cap (2), it is characterized in that: the bottom of bottle (1) is shaped to (3) between grid, and between grid, the quantity of (3) determines according to bottle sizes.
2. a kind of tissue culture bottle according to claim 1, it is characterized in that between grid, size is more even, vertical view at the bottom of its bottle: be square (4) between middle grid, between the grid adjacent with bottle edge, outer shape changes with bottle edge radian and changes (5), bottom side length 1.8 ~ 2.0cm between square lattice, high 2.5 ~ 3cm.
3. a kind of tissue culture bottle according to claim 2, jar base diameter 6.9 cm of capacity 370ml arranges between 16 grid (6), the osculum tissue culture bottle of capacity 650ml, and base diameter is 9.6cm, arranges between 25 grid (7).
4. a kind of tissue culture bottle according to claim 1,2 or 3, aperture (8) can be had to communicate above the sidewall that it is characterized in that (3) between grid, claim mutual-through type structure between the grid having aperture to communicate, claim stand-alone configuration without between the grid that aperture communicates.
5. a kind of tissue culture bottle according to claim 1, it is characterized in that between bottle (1) and grid, (3) material is transparent quartz glass, bottle cap is transparent plastic.
6. a kind of tissue culture bottle according to claim 1, is characterized in that bottleneck (9) diameter is less than bottle (1) diameter, and bottle cap (2) and bottleneck (9) are by screw thread mouth compact siro spinning technology.
7. a kind of tissue culture bottle according to claim 1, to is characterized in that between grid that (3) and bottle are combined as a whole structure.
8. a kind of tissue culture bottle according to claim 1, after it is characterized in that can making the overall structure (10) between bottle bottom and grid with mould when producing, then is bonded into overall bottle with bottle middle and upper part (11).
CN201420632253.6U 2014-10-29 2014-10-29 A kind of tissue culture bottle Expired - Fee Related CN204132129U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201420632253.6U CN204132129U (en) 2014-10-29 2014-10-29 A kind of tissue culture bottle

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201420632253.6U CN204132129U (en) 2014-10-29 2014-10-29 A kind of tissue culture bottle

Publications (1)

Publication Number Publication Date
CN204132129U true CN204132129U (en) 2015-02-04

Family

ID=52408866

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201420632253.6U Expired - Fee Related CN204132129U (en) 2014-10-29 2014-10-29 A kind of tissue culture bottle

Country Status (1)

Country Link
CN (1) CN204132129U (en)

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CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20150204

Termination date: 20171029