CN204128628U - The device of liquid measure in real-time detection flow cytometer liquid container - Google Patents
The device of liquid measure in real-time detection flow cytometer liquid container Download PDFInfo
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- CN204128628U CN204128628U CN201420151465.2U CN201420151465U CN204128628U CN 204128628 U CN204128628 U CN 204128628U CN 201420151465 U CN201420151465 U CN 201420151465U CN 204128628 U CN204128628 U CN 204128628U
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- load cells
- radial load
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- fluid path
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- 239000007788 liquid Substances 0.000 title claims abstract description 85
- 238000011897 real-time detection Methods 0.000 title claims description 9
- 239000012530 fluid Substances 0.000 claims abstract description 43
- 230000035945 sensitivity Effects 0.000 claims description 5
- 238000001514 detection method Methods 0.000 abstract description 10
- 239000002699 waste material Substances 0.000 description 7
- 238000004140 cleaning Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 238000007654 immersion Methods 0.000 description 4
- 238000009434 installation Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000004643 material aging Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to the device detecting liquid measure in flow cytometer liquid container in real time, radial LOAD CELLS is installed on the bottom of fluid path cabinet wall, the madial wall of fluid path casing is provided with spring card, liquid container bucket is placed in fluid path casing, the bottom surface of liquid container bucket contacts with radial LOAD CELLS, radial LOAD CELLS load bearing fluid drum, liquid container bucket is positioned between spring card, and the data line of radial LOAD CELLS is external to analog to digital converter.By adopting radial LOAD CELLS sense weight indirect detection liquid level, realizing the continuous level sensing of similar ultrasonic sensor, significantly reducing cost simultaneously.By the mode of continuous level sensing, even if sensor failure, whether operating personnel can observe sensor by the change of real-time liquid level and lose efficacy.Simplify the difficulty of mechanism's installment and debugging, can the container of compatible different volumes.
Description
Technical field
The utility model relates to the device that detects liquid measure in flow cytometer liquid container in real time.
Background technology
Liquid container in flow cytometer comprises sheath fluid bucket, waste liquid barrel and cleaning fluid bucket.Sheath fluid bucket splendid attire is for wrapping up deionized water, tri-distilled water or the PBS damping fluid (phosphate buffer) isotonic with cell of cell sample stream, sheath fluid is the carrier fluid of cell sample stream, and sheath fluid needs user in bucket, to supplement new sheath fluid in time when being finished; Waste liquid barrel is used for the complete waste collection of fluid path cycle detection, and waste liquid barrel is collected completely needs user to dispose in time; Cleaning fluid bucket is used for liquor natrii hypochloritis or the sheath fluid of splendid attire cleaning fluid path, and cleaning fluid needs user in bucket, to supplement new cleaning fluid in time when being finished.
Run in use procedure at daily flow cytometer, these three kinds of liquid containers are along with the carrying out of experiment, its internal liquid amount real-time change, this just needs the Real-Time Monitoring means providing a kind of liquid level or liquid capacity, with the fluid level condition in the liquid container that alarm reminding user is current, and take appropriate measures, carry out to prevent experiment in process, just finding that liquid level is not right, continuing of testing is hindered to carry out, light then need again to test and cause cell sample to be wasted, a large amount of bubble is produced in fluid path conduit, heavy then waste liquid barrel is overflowed and is caused fluid path operation irregularity, viral sample waste liquid enters cabinet and is difficult to clean and brings potential safety hazard.
In existing flow cytometer, in liquid container, liquid inspection technology has following shortcoming:
1) immobile liquid position detection method uses immersion floating-ball level switch, the height type selecting according to liquid container is needed during type selecting, different containers has different height, causes the length selecting different level switches, causes extra cost when container is changed.Immersion floating-ball level switch, due to the principle of work of its immersion, need keep regular cleaning and sterilizing work.And immersion floating-ball level switch can sacrifice the volume of a part of liquid container.
2) another mode of fixing level sensing installs proximity electric capacity or photoelectric liquid-levle detecting sensor switch at liquid container outer wall, this electronic sensor has different detection sensitivities to different detection media, as the transparency to bucket, liquid in bucket medium, detection distance of reaction have certain requirement, too increase the requirement to Installation and Debugging.And if the material aging of the attachment of bucket wall dust or bucket all can bring metrical error.
3) above-mentioned two kinds of detection methods are all the liquid-level detecting methods based on switching value, as needed the liquid level of inspection instrument 10% height and position, sensing switch is then needed to be arranged in this height and position, current liquid level position can not be reflected in real time to user, if during sensor failure, can not alarm in time, cause the situation of experimental error or discharge to occur.
4) ultrasonic liquid level sensor can detect liquid level in real time, but its Costco Wholesale is expensive, to compare more sensitive flow cytometer scheme for price, not too applicable.
Summary of the invention
The purpose of this utility model is the deficiency overcoming prior art existence, provides the device of liquid measure in a kind of real-time detection flow cytometer liquid container.
The purpose of this utility model is achieved through the following technical solutions:
The device of liquid measure in real-time detection flow cytometer liquid container, feature is: comprise fluid path casing, liquid container bucket and radial LOAD CELLS, described radial LOAD CELLS is installed on the bottom of fluid path cabinet wall, the madial wall of fluid path casing is provided with spring card, liquid container bucket is placed in fluid path casing, the bottom surface of liquid container bucket contacts with radial LOAD CELLS, radial LOAD CELLS load bearing fluid drum, liquid container bucket is positioned between spring card, and the data line of radial LOAD CELLS is external to analog to digital converter.
Further, in above-mentioned real-time detection flow cytometer liquid container, the device of liquid measure, is characterized in that: described radial LOAD CELLS is screwed the bottom in fluid path cabinet wall.
Further, the device of liquid measure in above-mentioned real-time detection flow cytometer liquid container, the radial LOAD CELLS of described radial LOAD CELLS to be output sensitivity be 2mV/V.
Substantive distinguishing features and the progress of technical solutions of the utility model are mainly reflected in:
By adopting radial LOAD CELLS sense weight indirect detection liquid level, realizing the continuous level sensing of similar ultrasonic sensor, significantly reducing cost simultaneously.By the mode of continuous level sensing, even if sensor failure, whether operating personnel, by the change of real-time liquid level, still can observe sensor and lose efficacy.By using radial LOAD CELLS, greatly simplifie the difficulty of mechanism's installment and debugging.By using radial LOAD CELLS, the profile of the liquid container that flow cytometer is used, without any restriction, to be weighed scope by the difference of type selecting LOAD CELLS, can the container of compatible different volumes.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, technical solutions of the utility model are described further:
Fig. 1: organigram of the present utility model.
Embodiment
As shown in Figure 1, the device of liquid measure in real-time detection flow cytometer liquid container, comprise fluid path casing 1, liquid container bucket 2 and radial LOAD CELLS 4, radial LOAD CELLS 4 is fixed on the bottom of fluid path cabinet wall by screw 5, the madial wall of fluid path casing 1 is provided with spring card 3, liquid container bucket 2 is placed in fluid path casing 1, the bottom surface of liquid container bucket 2 contacts with radial LOAD CELLS 4, radial LOAD CELLS 4 load bearing fluid drum 2, liquid container bucket 2 is positioned between spring card, the data line 6 of radial LOAD CELLS 4 is external to analog to digital converter, the radial LOAD CELLS of radial LOAD CELLS 4 to be output sensitivities be 2mV/V.
During embody rule, quality or weight signal can be changed into direct measurable electric signal (normally the voltage signal of analog quantity) and export by radial LOAD CELLS 4, by detecting this voltage signal, according to the voltage-quality linear relation of sensor, converse the weight of liquid container.Can the amount of liquid in real time reaction liquid container bucket 2 by the weight measuring liquid container bucket 2 (containing internal liquid).The mounting means of radial LOAD CELLS 4 is succinct, screw hole fixed installation only need be opened on the bottom of fluid path casing 1, need carry out perforate in liquid container upper end and seal fixed installation unlike floating-ball level switch, also do not need to need to consider that the extra mechanism's fixed mount of increase or retainer plate are close to be arranged on container outer wall as condenser type, electro-optical pickoff, ultrasonic sensor.
Be fixed on bottom fluid path casing 1 bottom radial LOAD CELLS 4, fluid path casing 1 sidewall mounting spring card 3, bucket wall and the spring card 3 of liquid container bucket 2 keep minim gap, for preventing deflection to the spacing of liquid container bucket 2.
By adopting radial LOAD CELLS 4 sense weight indirect detection liquid level, realizing the continuous level sensing of similar ultrasonic sensor, significantly reducing cost simultaneously.By the mode of continuous level sensing, even if sensor failure, whether operating personnel, by real-time liquid level change (measurement by output voltage), still can observe sensor and lose efficacy.By using radial LOAD CELLS, greatly simplifie the difficulty of mechanism's installment and debugging.By using radial LOAD CELLS, the profile of the liquid container that flow cytometer is used, without any restriction, to be weighed scope by the difference of type selecting LOAD CELLS, can the container of compatible different volumes.
Radial LOAD CELLS 4 good rigidly, lateral force resistance is strong, and output sensitivity reaches 2mV/V, and protection adopts rubber seal and sealing, and degree of protection reaches IP67 and IP68, and reliability is high, is applicable to the fluid path environment of flow cytometer completely.
Solving existing liquid-level detecting method can only a certain fixing liquid level of fixed test, can only do the detection of discrete magnitude, can not the situation of liquid level in real time reaction current container.Simplify existing liquid-level detecting method to liquid level sensor type selecting, debugging and installation question.Avoiding existing liquid-level detecting method normally causes experiment time-out, sample waste, pipeline to suck the appearance of the situation such as bubble, discharge because sensor failure causes user to judge fluid level condition by accident.Avoid the use of extremely expensive ultrasonic liquid level sensor.
It is emphasized that: be only preferred embodiment of the present utility model above, not any pro forma restriction is done to the utility model, every above embodiment is done according to technical spirit of the present utility model any simple modification, equivalent variations and modification, all still belong in the scope of technical solutions of the utility model.
Claims (3)
1. detect the device of liquid measure in flow cytometer liquid container in real time, it is characterized in that: comprise fluid path casing, liquid container bucket and radial LOAD CELLS, described radial LOAD CELLS is installed on the bottom of fluid path cabinet wall, the madial wall of fluid path casing is provided with spring card, liquid container bucket is placed in fluid path casing, the bottom surface of liquid container bucket contacts with radial LOAD CELLS, radial LOAD CELLS load bearing fluid drum, liquid container bucket is positioned between spring card, and the data line of radial LOAD CELLS is external to analog to digital converter.
2. the device of liquid measure in real-time detection flow cytometer liquid container according to claim 1, is characterized in that: described radial LOAD CELLS is screwed the bottom in fluid path cabinet wall.
3. the device of liquid measure in real-time detection flow cytometer liquid container according to claim 1, is characterized in that: the radial LOAD CELLS of described radial LOAD CELLS to be output sensitivity be 2mV/V.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420151465.2U CN204128628U (en) | 2014-04-01 | 2014-04-01 | The device of liquid measure in real-time detection flow cytometer liquid container |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420151465.2U CN204128628U (en) | 2014-04-01 | 2014-04-01 | The device of liquid measure in real-time detection flow cytometer liquid container |
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| Publication Number | Publication Date |
|---|---|
| CN204128628U true CN204128628U (en) | 2015-01-28 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420151465.2U Expired - Lifetime CN204128628U (en) | 2014-04-01 | 2014-04-01 | The device of liquid measure in real-time detection flow cytometer liquid container |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106441478A (en) * | 2016-11-28 | 2017-02-22 | 惠州市广工大物联网协同创新研究院有限公司 | Automatic liquid measuring device and method |
| CN108871495A (en) * | 2018-06-05 | 2018-11-23 | 江苏卓微生物科技有限公司 | A kind of liquid level detection system and liquid-level detecting method |
| CN109682442A (en) * | 2019-01-14 | 2019-04-26 | 东莞市凯格精密机械有限公司 | A kind of the Weighing type balance of solvent detection device and detection method of printing machine |
-
2014
- 2014-04-01 CN CN201420151465.2U patent/CN204128628U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106441478A (en) * | 2016-11-28 | 2017-02-22 | 惠州市广工大物联网协同创新研究院有限公司 | Automatic liquid measuring device and method |
| CN108871495A (en) * | 2018-06-05 | 2018-11-23 | 江苏卓微生物科技有限公司 | A kind of liquid level detection system and liquid-level detecting method |
| CN109682442A (en) * | 2019-01-14 | 2019-04-26 | 东莞市凯格精密机械有限公司 | A kind of the Weighing type balance of solvent detection device and detection method of printing machine |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20150128 |
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| CX01 | Expiry of patent term |