CN204121469U - A kind of nanotopography chip with induced cell proliferation differentiation capability - Google Patents
A kind of nanotopography chip with induced cell proliferation differentiation capability Download PDFInfo
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- CN204121469U CN204121469U CN201420474426.6U CN201420474426U CN204121469U CN 204121469 U CN204121469 U CN 204121469U CN 201420474426 U CN201420474426 U CN 201420474426U CN 204121469 U CN204121469 U CN 204121469U
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- chip
- nanotopography
- cell proliferation
- induced cell
- differentiation capability
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Abstract
The utility model discloses a kind of nanotopography chip with induced cell proliferation differentiation capability, stem cell or bone cell proliferation direction can be guided and regulate it to break up, accelerate bone tissue restoration.Comprise the substrate of chromium sheet, the substrate of chromium sheet is provided with some silicon chip unit, silicon chip unit is placed with the micrographics that nanoscale pattern is carved with on surface.Micro-lines pattern section of this utility model chip surface guides stem cell or osteocyte to breed along the direction arrangement of micro-lines, this directional proliferation adds the growth efficiency of cell, thus promote that osteoblast is assembled to Cranial defect direction, form the callus by fracture line as early as possible, the final repair process accelerating Cranial defect.
Description
Technical field
This utility model belongs to medical supplies, particularly a kind of nanotopography chip with induced cell proliferation differentiation capability.
Background technology
Bone wound and the Cranial defect caused thereof often perplex Orthopedic Clinical work, although bone collection can solve the case of some complexity, the complication such as its infection of bone, Qu Gu district Cranial defect are inevitable.Autologous bone transplanting section reduces incidence of rejection, but due to needs Repeated Operation, and gets the bony site problem such as limited of drawing materials and annoyings the clinical treatment of Cranial defect.
For a long time, large quantity research concentrates on the combination interface of implantation body and osseous tissue, namely modifies to material surface the biological activity changing material surface by the mode such as physics, chemistry.From bionics angle, imitate extracellular matrix biophysical properties and also carry out finishing, can physical stimulation be produced thus affect the biological behaviour of cell.Multiple physical signalling in cell peripheral environment is converted into biochemical signals by simple mechanical force.The orientation of growth of cell can be sprawled along groove pattern trend and be moved, and this characteristic is named as contact induction (contact guidance).By this characteristic, without the need to chemical modification with regard to the biological behaviour of controllable cell and function.
Existing foreign scholar Hamilton etc. adopt the trench material that 130nm is wide, 500nm is dark at present, Mus mescenchymal stem cell is cultivated on its surface, observe after cultivating, in orientations, not only there is orientations in the flute surfaces that Primary chondrocyte is wide at 750nm, 80-90nm is dark, and cell migration speed significantly increases.
Utility model content
The purpose of this utility model is to provide a kind of nanotopography chip with induced cell proliferation differentiation capability, can guide stem cell or bone cell proliferation direction and regulate it to break up, and accelerates bone tissue restoration.
For achieving the above object, this utility model is by the following technical solutions:
There is a nanotopography chip for induced cell proliferation differentiation capability, comprise the substrate of chromium sheet, the substrate of chromium sheet is provided with some silicon chip unit, silicon chip unit is placed with the micrographics that nanoscale pattern is carved with on surface.
Separated by wall between adjacent silicon chip unit.
Described micrographics by cross section be triangle, circle and square three kinds of shapes post arrange form.
Described triangle is isosceles triangle, and angle is respectively 36 °, 72 ° and 72 °.
The described square length of side is 3 μm.
The degree of depth of micrographics is 5 μm.
Spacing between adjacent micrographics is 1 μm.
The overall size of described chromium sheet substrate is 2cm × 2cm.
The size of silicon chip unit is 290 μm × 290 μm.
The height of wall is 5-20 μm.
The beneficial effects of the utility model are:
1) this surface treatment mode does not add extra chemical constituent, biological safety is good, do not rely on biological active component, cost is lower, the course of processing is computer-controlled artistic carving, forming process automaticity is high, output is large, and the microarray that machining obtains is idiosome metal material not yet, possess can high-temperature sterilization, firm feature.
2) chip surface morphology can have contact inducing action, in-vitro screening is carried out by each parameter of effects on surface array, formally only have the microarray surface of particular design can obtain desirable effect of stimulation to stem cell, thus we are by the micrographics processing implantation material surface after optimal screening, can provide strong bonded connective tissue and the effect stimulating stem cell directional to grow.
3) along with the success rate of implantable bone defect healing operation improves constantly, obtain good combination in operation after, to the stable support of surrounding tissue and the guiding function of osteocyte being migrated to direction in long-term implantation process, it is the key factor affecting Bone Defect Repari success rate at a specified future date.Micro-lines pattern section of chip surface of the present invention guides stem cell or osteocyte to breed along the direction arrangement of micro-lines, this directional proliferation adds the growth efficiency of cell, thus promote that osteoblast is assembled to Cranial defect direction, form the callus by fracture line as early as possible, the final repair process accelerating Cranial defect.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 is scanning electron microscope (SEM) photograph of the present invention;
Fig. 3 is the scanning electron microscope (SEM) photograph of local of the present invention;
Fig. 4 is the arrangement schematic diagram of silicon chip unit.
Detailed description of the invention
Below in conjunction with accompanying drawing, this utility model is described further.
As Figure 1-4, a kind of nanotopography chip with induced cell proliferation differentiation capability, comprise chromium sheet substrate 1, size is 2cm × 2cm, chromium sheet substrate 1 is provided with some silicon chip unit 2, size is 290 μm × 290 μm, silicon chip unit 2 is placed with the micrographics 4 that nanoscale pattern is carved with on surface, passes through highly for the wall 3 of 5-20 μm separates between adjacent silicon chip unit 2.
Micrographics 4 by cross section be triangle, circle and square three kinds of shaped formation form, triangle is isosceles triangle, and angle is respectively 36 °, 72 ° and 72 °, and the square length of side is 3 μm.The degree of depth of micrographics 4 is 5 μm, and the spacing between adjacent micrographics 4 is 1 μm.
The method making this micro-lines pattern has a variety of, and these preparation methoies can be divided into two classes: a class mode etches micro groove at original surface; Another kind of mode is on original surface, build dimpling ridge.First kind method by etching method, can comprise the method for method by laser-induced thermal etching or chemical etching.Equations of The Second Kind method also has a variety of, can by plating or by magnetron sputtering plating or pass through chemical deposition.The present invention preferentially realizes the preparation of micro-lines by the method for laser-induced thermal etching, comprise the following steps specifically:
Step 1 prepares implant body original paper;
Step 2 laser goes out micro-lines pattern at the surface etching of implant body under control of the computer;
Step 3 removes the burr of implant surface by polishing or chemical method.
Because the preparation method of this micro-lines is a lot, the chip specifically how prepared with the micro-lines in surface does not have an impact to product structure of the present invention, does not affect the present invention.
Embodiment 1 proliferation test
HESC HN4(SCSP-303 with commercially available) for object of study, and with the common chip without nanotopography for contrast, carry out the experimental study of stem cell proliferative induction, concrete grammar is as follows:
1) the nanotopography chip that has prepared is put into autoclave high temperature sterilize together with contrast chip;
2) 24h prepares MEF cell in advance: bag is by Matrigel in advance, by 8 × 10
5the amount paving MEF cell of/T25, uses 10%DMEM.HES complete culture solution is replaced by after upper hES cell to be seeded;
3), after adjusting HN4 cell concentration, access with condition according to a conventional method simultaneously and there is nanotopography chip and contrast chip, temperature: 37 DEG C, gas phase: air 95%, carbon dioxide 5%, change liquid frequency: every day, carry out proliferative induction cultivation.
Cultivate observed result after 20 days, find that the stem cell with nanotopography chip surface is along the direction arrangement propagation of micro-lines, adds the growth efficiency of cell, compared with control sample, about increase by 20% growth rate, cut contrast chip proliferate direction disorderly and unsystematic, irregular.
Adopt same method, the discovery of proliferative induction contrast test is carried out to osteocyte, there is the direction arrangement propagation of osteocyte along micro-lines of nanotopography chip surface, add the growth efficiency of cell, compared with control sample, about increase by 15% growth rate, cut contrast chip proliferate direction disorderly and unsystematic, irregular.
Claims (10)
1. one kind has the nanotopography chip of induced cell proliferation differentiation capability, it is characterized in that: comprise chromium sheet substrate (1), chromium sheet substrate (1) is provided with some silicon chip unit (2), silicon chip unit (2) is placed with the micrographics (4) that nanoscale pattern is carved with on surface.
2. there is the nanotopography chip of induced cell proliferation differentiation capability as claimed in claim 1, it is characterized in that: separated by wall (3) between adjacent silicon chip unit (2).
3. there is the nanotopography chip of induced cell proliferation differentiation capability as claimed in claim 1, it is characterized in that: described micrographics (4) by cross section be triangle, circle and square three kinds of shapes post arrange form.
4. have the nanotopography chip of induced cell proliferation differentiation capability as claimed in claim 3, it is characterized in that: described triangle is isosceles triangle, angle is respectively 36 °, 72 ° and 72 °.
5. the nanotopography chip with induced cell proliferation differentiation capability as described in claim 3 or 4, is characterized in that: the degree of depth of micrographics (4) is 5 μm.
6. there is the nanotopography chip of induced cell proliferation differentiation capability as claimed in claim 5, it is characterized in that: the spacing between adjacent micrographics (4) is 1 μm.
7. there is the nanotopography chip of induced cell proliferation differentiation capability as claimed in claim 1, it is characterized in that: the length and width of described chromium sheet substrate (1) are of a size of 2cm × 2cm.
8. there is the nanotopography chip of induced cell proliferation differentiation capability as claimed in claim 1, it is characterized in that: the length and width of silicon chip unit (2) are of a size of 290 μm × 290 μm.
9. there is the nanotopography chip of induced cell proliferation differentiation capability as claimed in claim 2, it is characterized in that: the height of described wall (3) is 5-20 μm.
10. there is the nanotopography chip of induced cell proliferation differentiation capability as claimed in claim 2, it is characterized in that: the described square length of side is 3 μm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420474426.6U CN204121469U (en) | 2014-03-11 | 2014-08-22 | A kind of nanotopography chip with induced cell proliferation differentiation capability |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420108446.1 | 2014-03-11 | ||
| CN201420108446 | 2014-03-11 | ||
| CN201420474426.6U CN204121469U (en) | 2014-03-11 | 2014-08-22 | A kind of nanotopography chip with induced cell proliferation differentiation capability |
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| CN204121469U true CN204121469U (en) | 2015-01-28 |
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| CN201420474426.6U Expired - Fee Related CN204121469U (en) | 2014-03-11 | 2014-08-22 | A kind of nanotopography chip with induced cell proliferation differentiation capability |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103861155A (en) * | 2014-03-11 | 2014-06-18 | 郑欣 | Nano-topography chip with capacity of inducing cell proliferation and differentiation |
| CN106834221A (en) * | 2017-01-20 | 2017-06-13 | 常州大学 | A kind of new method that simulation human body tubular structure curved surface micrographics chip is prepared based on the uneven characteristic of 3D printing body surface |
| CN109310502A (en) * | 2016-02-16 | 2019-02-05 | 马特里优米克斯有限公司 | For changing the surface topography of living cells physiological function |
-
2014
- 2014-08-22 CN CN201420474426.6U patent/CN204121469U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103861155A (en) * | 2014-03-11 | 2014-06-18 | 郑欣 | Nano-topography chip with capacity of inducing cell proliferation and differentiation |
| CN109310502A (en) * | 2016-02-16 | 2019-02-05 | 马特里优米克斯有限公司 | For changing the surface topography of living cells physiological function |
| CN106834221A (en) * | 2017-01-20 | 2017-06-13 | 常州大学 | A kind of new method that simulation human body tubular structure curved surface micrographics chip is prepared based on the uneven characteristic of 3D printing body surface |
| CN106834221B (en) * | 2017-01-20 | 2020-04-24 | 常州大学 | Novel method for preparing human body tubular structure curved surface micro-graphic simulation chip based on 3D printed object surface unevenness characteristics |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150128 Termination date: 20180822 |