CN204047604U - A kind of zebra fish electroshock seizure test device - Google Patents
A kind of zebra fish electroshock seizure test device Download PDFInfo
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- CN204047604U CN204047604U CN201420443838.3U CN201420443838U CN204047604U CN 204047604 U CN204047604 U CN 204047604U CN 201420443838 U CN201420443838 U CN 201420443838U CN 204047604 U CN204047604 U CN 204047604U
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- container
- copper coin
- electrode copper
- zebra fish
- discharge outlet
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The utility model belongs to animal experiment equipment technical field, relates to a kind of zebra fish electroshock seizure test device, is applicable to electro photoluminescence zebra fish in water and carries out experimental study; Agent structure comprises egersimeter, separator, logger, container, the first electrode copper coin, the second electrode copper coin, discharge outlet, knots and three-way control valve, egersimeter one end ground connection, the other end is electrically connected with the first electrode copper coin on container and the second electrode copper coin respectively by separator, and the first electrode copper coin and the second electrode copper coin are fixedly installed on two ends, the left and right wall of container respectively; Logger is electrically connected with separator and container respectively; The side, bottom of container is fixedly installed discharge outlet, and discharge outlet is embedded with knotting of soft web frame, discharge outlet is connected with external source by three-way control valve; Its apparatus structure is reasonable, and principle is reliable, and easy and simple to handle, with low cost, safe and reliable during use, environmental friendliness, parameters of electrical stimulation is clear and definite.
Description
Technical field:
The utility model belongs to animal experiment equipment technical field, be specifically related to a kind of zebra fish electroshock seizure test device, be applicable to electro photoluminescence zebra fish in water and carry out experimental study, realize electro photoluminescence in administration in water, water simultaneously and observe in water and record its behavioral indicator, the fields such as teaching, scientific research can be widely used in.
Background technology:
Anticonvulsion test is common pharmacodynamic study project, also be the important content of pharmacological experiment teaching, traditional electrofit experimental model often selects mouse as subjects, but rodent mammal muroid is used for experiment, Financial cost is larger, degree of safety is low, and rearing conditions is high, inefficiency, and zebra fish is owing to cultivating conveniently, breeding cycle is short, egg laying amount is large, embryo is in vitro fertilization, ectogenesis, the plurality of advantages such as the transparent and mutant species of idiosome is many, and the similarity of zebra fish and human gene is up to 87%, it has been put into the third-largest model organism after people and mouse, current zebra fish replaces mammal gradually and becomes domestic and international popular study of pharmacy instrument, the national expenditures zebra fishs such as USA and Europe evaluate medicine also by GLP certification, when in the research of neontology, medical science and pharmacy test, zebra fish substitutes the third model animal that traditional mammal progressively becomes popular, but yet there are no the correlation test equipment of technology maturation.
Therefore the utility model is sought to design a kind of zebra fish multifunction test device, to fill up the blank of this technical field, realize carrying out electricity to zebra fish in water to shy and jerk and the experimental study such as electrofit, can carry out administration and observed behavior index simultaneously and record related data in water, it can be widely used in the fields such as teaching, scientific research; Concentrating on studies through inventor, this experimental rig successfully solves the interference to result of the test of water quality, the water yield, electric current and administering mode, and gives record to zebra fish in the frightened behavioural characteristic in reacting with convulsions of jerking by video or Photoelectric behavior logger; This experimental rig is used for zebra fish in water, to carry out electro photoluminescence experiment contribute to Process in Pharmacology Teaching test and the screening of antiepileptic, there is higher practice value and good economic benefit.
Summary of the invention:
The purpose of this utility model is to overcome the shortcoming that prior art exists, and seeks design and provides a kind of zebra fish electrofit experimental rig, can be widely used in during pharmacodynamic study and Process in Pharmacology Teaching test.
To achieve these goals, the experimental rig agent structure that the utility model relates to comprises egersimeter, separator, logger, container, the first electrode copper coin, the second electrode copper coin, discharge outlet, knots and three-way control valve, egersimeter one end ground connection, the other end is electrically connected with the first electrode copper coin on container and the second electrode copper coin respectively by separator, first electrode copper coin and the second electrode copper coin are fixedly installed on two ends, the left and right wall of container respectively, to realize giving electro photoluminescence to subjects zebra fish in container; Logger is electrically connected with separator and container respectively, to realize the record of correlation test data; The side, bottom of container is fixedly installed discharge outlet, discharge outlet is embedded with knotting of soft web frame, and to prevent zebra fish from swimming out of with drainpipe, discharge outlet is communicated with to realize water filling and drain function with external source by conventional three-way control valve.
Container described in the utility model selects box-shaped, tubulose annular or Straight transparent vessel, and the one or both ends wall of container can horizontally slip along bottom side, to realize the adjustment of length to container or width; Container maybe can select long tube, bend pipe or U-shaped pipe, adopts manual mode to carry out water filling and draining by extraneous conventional syringe to container; Logger selects commercially available video recorder or photoelectric recorder.
The utility model compared with prior art, broken traditional neural active medicine screening and dose-response curve select mammiferous pattern, this experimental rig take zebra fish as subjects, and under can observing the anti-convulsant activity result of the test of antiepileptic and traditional mode, mouse matches in the anticonvulsion result of maximal electrical seizure; This device can realize observing in administration in water, water moderate stimulation, water when using and record the integration operations such as corelation behaviour characteristic, can not only direct vision to the frightened reaction of jerking of noxious stimulation (electro photoluminescence, pain, stress wait) zebra fish performance, the convulsions characteristic reaction after electro photoluminescence, dying recovery and death process and toxic reaction etc. can also be observed, record can be carried out to related data simultaneously, therefore new medicament screen and pharmacy education experiment are with a wide range of applications; This apparatus structure is reasonable, and principle is reliable, and easy and simple to handle, with low cost, safe and reliable during use, environmental friendliness, parameters of electrical stimulation is clear and definite.
Accompanying drawing illustrates:
Fig. 1 is agent structure module principle schematic diagram of the present utility model.
Fig. 2 is the reference configuration exemplary plot of the container related to of the present utility model.
Embodiment:
By embodiment, further description is made to the utility model below in conjunction with accompanying drawing.
Embodiment 1:
The agent structure of the present embodiment comprises egersimeter 1, separator 2, logger 3, container 4, first electrode copper coin 5, second electrode copper coin 6, discharge outlet 7, knot 8 and three-way control valve 9, egersimeter 1 one end ground connection, the other end is electrically connected with the first electrode copper coin 5 on container 4 and the second electrode copper coin 6 respectively by separator 2, first electrode copper coin 5 and the second electrode copper coin 6 are fixedly installed on two ends, the left and right wall of container 4 respectively, to realize giving electro photoluminescence to subjects zebra fish in container 4; Logger 3 is electrically connected with separator 2 and container 4 respectively, to realize the record of correlation test data; The side, bottom of container 4 is fixedly installed discharge outlet 7, and what discharge outlet 7 is embedded with soft web frame knots 8, and discharge outlet 7 is communicated with to realize water filling and drain function with external source by conventional three-way control valve 9.
The container 4 related in the present embodiment selects box-shaped, tubulose annular or Straight transparent vessel, and its one or both ends wall can horizontally slip along bottom side, to realize the adjustment of length to container 4 or width; Described container 4 also can select long tube, bend pipe or U-shaped pipe, then adopt manual mode to carry out water filling and draining by conventional syringe to container; Logger 3 selects commercially available video recorder or photoelectric recorder; Egersimeter 1 can select domestic on market or import egersimeter; Domestic on market or import electro photoluminescence separator selected by separator 2.
Embodiment 2:
The present embodiment carries out zebra fish electro photoluminescence behavioral study to use this device, and select transparent straight glass tube as container 4, correlation test step is as follows:
(1) test material: zebra fish 40, male and female have concurrently, body weight 240 ± 20mg, random packet (n=10), free diet, room temperature 23 DEG C, natural lighting round the clock; Select diameter 0.8cm, long 10cm, volume be the transparent straight glass tube of 5ml as container 4, and combine installation cost experimental rig with miscellaneous parts such as egersimeter 1, separator 2 and loggers 3;
(2) test method: test filtered out the zebra fish of 50 μ A current response sensitivities for test the day before yesterday, every bar test is placed in 100mL beaker respectively with zebra fish raises, during test, every bar test is placed in container 4 respectively with zebra fish, to be tested conform with zebra fish after static time, control the electrical stimulation signal that egersimeter 1 gives different current, then the viewing test behavior reaction of zebra fish, and record the behavior reaction related data of zebra fish by logger 3 or artificial direct visual perception, test is all carried out in the morning;
(3) result of the test: after the galvanism 1s of 50 μ A-100 μ A, alter, jump before test has with zebra fish, the characteristic behavior such as whipping or convolution, be referred to as temporarily " frightened jerk reaction " (it is that the guarding reflex that a kind of physiology of noxious stimulation is escaped is movable that reflex action of jerking is shied in the electro photoluminescence of fish, noxious stimulation also comprise pain, scaring stress with the stress stimulation injuring body such as to be overexcited); After the galvanism 1s of 2000 μ A, test and occur A condition with zebra fish: whole body is straight and upright, motionless, then occur B state: rollover, stupor or dead, be referred to as temporarily " reaction of fainting from fear ", concrete outcome is as shown in table 1:
Table 1
Note: table 1 is the impact (n=10) of electric current on zebra fish behavior of different size.
Claims (3)
1. a zebra fish electroshock seizure test device, it is characterized in that agent structure comprises egersimeter, separator, logger, container, the first electrode copper coin and the second electrode copper coin, egersimeter one end ground connection, the other end is electrically connected with the first electrode copper coin on container and the second electrode copper coin respectively by separator, and the first electrode copper coin and the second electrode copper coin are fixedly installed on two ends, the left and right wall of container respectively; The side, bottom of container is fixedly installed discharge outlet, and discharge outlet is embedded with knotting of soft web frame, discharge outlet is communicated with external source by three-way control valve; Logger is electrically connected with separator and container respectively.
2. zebra fish electroshock seizure test device according to claim 1, it is characterized in that described container is box-shaped, tubulose annular or Straight transparent vessel, the one or both ends wall of container can horizontally slip along bottom side.
3. zebra fish electroshock seizure test device according to claim 1, is characterized in that described container or selects long tube, bend pipe or U-shaped pipe, carry out water filling and draining by extraneous syringe to container.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420443838.3U CN204047604U (en) | 2014-08-08 | 2014-08-08 | A kind of zebra fish electroshock seizure test device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420443838.3U CN204047604U (en) | 2014-08-08 | 2014-08-08 | A kind of zebra fish electroshock seizure test device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204047604U true CN204047604U (en) | 2014-12-31 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420443838.3U Withdrawn - After Issue CN204047604U (en) | 2014-08-08 | 2014-08-08 | A kind of zebra fish electroshock seizure test device |
Country Status (1)
| Country | Link |
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| CN (1) | CN204047604U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104137800A (en) * | 2014-08-08 | 2014-11-12 | 青岛中仁药业有限公司 | Zebra fish electroconvulsive testing device |
| CN106855560A (en) * | 2017-02-28 | 2017-06-16 | 山东省科学院生物研究所 | The device and its method of work of a kind of Fast Evaluation compound acute toxicity |
-
2014
- 2014-08-08 CN CN201420443838.3U patent/CN204047604U/en not_active Withdrawn - After Issue
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104137800A (en) * | 2014-08-08 | 2014-11-12 | 青岛中仁药业有限公司 | Zebra fish electroconvulsive testing device |
| CN104137800B (en) * | 2014-08-08 | 2016-02-24 | 青岛中仁药业有限公司 | A kind of zebra fish electroshock seizure test device |
| CN106855560A (en) * | 2017-02-28 | 2017-06-16 | 山东省科学院生物研究所 | The device and its method of work of a kind of Fast Evaluation compound acute toxicity |
| CN106855560B (en) * | 2017-02-28 | 2023-07-21 | 山东省科学院生物研究所 | Device for rapidly evaluating acute toxicity of compound and working method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C53 | Correction of patent for invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Yue Wang Inventor after: Yue Chuan Inventor after: Xia Wei Inventor after: Zhang Shuzhi Inventor after: Zhou Tao Inventor after: Yang Jie Inventor after: Li Xiaoling Inventor after: Zhang Jiguo Inventor after: Geng Bo Inventor after: Liu Zhantao Inventor after: Xu Quanhan Inventor after: Su Dongmei Inventor before: Yue Wang Inventor before: Yue Chuan Inventor before: Xia Wei Inventor before: Zhou Shuzhi Inventor before: Zhou Tao Inventor before: Yang Jie Inventor before: Li Xiaoling Inventor before: Zhang Jiguo Inventor before: Geng Bo Inventor before: Liu Zhantao Inventor before: Xu Quanhan Inventor before: Su Dongmei |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: YUE WANG ZHOU TAO YANG JIE LI XIAOLING ZHANG JIGUO GENG BO LIU ZHANTAO XU QUANHAN SU DONGMEI YUE CHUAN XIA WEI ZHOU SHUZHI TO: YUE WANG ZHOU TAO YANG JIE LI XIAOLING ZHANG JIGUO GENG BO LIU ZHANTAO XU QUANHAN SU DONGMEI YUE CHUAN XIA WEI ZHANG SHUZHI |
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| AV01 | Patent right actively abandoned |
Granted publication date: 20141231 Effective date of abandoning: 20160224 |
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| C25 | Abandonment of patent right or utility model to avoid double patenting |