CN204008269U - A kind of a small amount of fresh tobacco leaf metabolism group fast sampling sample freezing storage device - Google Patents
A kind of a small amount of fresh tobacco leaf metabolism group fast sampling sample freezing storage device Download PDFInfo
- Publication number
- CN204008269U CN204008269U CN201420431713.9U CN201420431713U CN204008269U CN 204008269 U CN204008269 U CN 204008269U CN 201420431713 U CN201420431713 U CN 201420431713U CN 204008269 U CN204008269 U CN 204008269U
- Authority
- CN
- China
- Prior art keywords
- centrifuge tube
- sample
- liquid nitrogen
- small amount
- storage device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241000208125 Nicotiana Species 0.000 title claims abstract description 22
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 22
- 238000005070 sampling Methods 0.000 title claims abstract description 21
- 230000004060 metabolic process Effects 0.000 title claims abstract description 18
- 238000007710 freezing Methods 0.000 title claims abstract description 11
- 230000008014 freezing Effects 0.000 title claims abstract description 11
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 38
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 19
- 238000012360 testing method Methods 0.000 claims abstract description 18
- 238000004080 punching Methods 0.000 abstract description 8
- 238000004321 preservation Methods 0.000 abstract description 3
- 230000009172 bursting Effects 0.000 abstract description 2
- 238000001816 cooling Methods 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 3
- 235000019504 cigarettes Nutrition 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 230000000873 masking effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 235000011089 carbon dioxide Nutrition 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 238000011020 pilot scale process Methods 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
A kind of a small amount of fresh tobacco leaf metabolism group fast sampling sample freezing storage device, it is characterized in that: comprise that one is loaded with the bubble chamber of liquid nitrogen and is arranged on the test tube fixed mount in bubble chamber, on test tube fixed mount, be placed with the centrifuge tube of a plurality of 50ml, each centrifuge tube is equipped with pipe lid and centrifuge tube bottom is equipped with open-work, the height of open-work when the liquid level of liquid nitrogen is greater than centrifuge tube and is placed on test tube fixed mount.Feature of the present utility model is: sample after punching sampling can be immersed to liquid nitrogen immediately, stop sample metabolic process, guarantee the authenticity of sample; Sample storage, in centrifuge tube, is convenient to later stage preservation and takes simultaneously; Centrifuge tube punching can prevent that centrifuge tube from bursting when liquid nitrogen takes out and also can make liquid nitrogen enter rapidly the interior instant cooling sample of pipe.
Description
Technical field
The utility model relates to the preservation apparatus in the sampling process of tobacco land for growing field crops, specifically a kind of a small amount of fresh tobacco leaf metabolism group fast sampling sample freezing storage device.
Background technology
Tobacco metabolism group is that research tobacco grows and quality of tobacco forms the new branch of science of material base, is the useful and necessary complement of functional genomics.Tobacco metabolism group is mainly studied the compound that in tobacco leaf, molecular weight is less than 1000, great majority are secondary metabolites, this class material is mainly the product that cigarette strain and environment are done mutually, comprise a lot of flavor precursors, be the important determinative of quality of tobacco, study its metabolic rule and regulatory factor thereof and regulated and control network significant for tobacco harm reduction and quality-improving; Tobacco metabolism group is affected by environment large simultaneously, small environmental stimuli all can show at metaboilic level, therefore metabolism group research key factor is representativeness and the typicalness of sample, after requiring sample in vitro, will stop as early as possible its metabolic process, general method is that after sample collection, masking foil is wrapped rear liquid nitrogen flash freezer at present.It is fine that yet the method is prepared effect for the collection of a large amount of samples, and a small amount of sampling has a lot of problems for card punch: first, punching sampling belongs to mechanical wounding, the sample volume of punching sampling is simultaneously little, tobacco leaf stress reaction will produce rapidly, according to traditional approach, preserve sample, can not actual response cigarette strain physiological status; Very inconvenient when after masking foil packing sample, sample is taken again simultaneously, may cause sample to thaw, the authenticity of impact analysis data.Therefore, researcher needs a kind of device that can immediately carry out quick-frozen to punching sampling of design badly.
Summary of the invention
The purpose of this utility model has overcome the deficiencies in the prior art and quick-frozen and the save set of a kind of quick and easy punching tobacco sample that provides just--a small amount of fresh tobacco leaf metabolism group fast sampling sample freezing storage device.
The purpose of this utility model is achieved through the following technical solutions:
A kind of a small amount of fresh tobacco leaf metabolism group fast sampling sample freezing storage device, comprise that one is loaded with the bubble chamber of liquid nitrogen and is arranged on the test tube fixed mount in bubble chamber, on test tube fixed mount, be placed with the centrifuge tube of a plurality of 50ml, each centrifuge tube is equipped with pipe lid and centrifuge tube bottom is equipped with open-work, the height of open-work when the liquid level of liquid nitrogen is greater than centrifuge tube and is placed on test tube fixed mount.
Described test tube fixed mount is two row's formula test tube racks; Described centrifuge tube is tip centrifuge tube, and the open-work aperture on described centrifuge tube is 1-2mm.
Feature of the present utility model is: sample after punching sampling can be immersed to liquid nitrogen immediately, stop sample metabolic process, guarantee the authenticity of sample; Sample storage, in centrifuge tube, is convenient to later stage preservation and takes simultaneously; Centrifuge tube punching can prevent that centrifuge tube from bursting when liquid nitrogen takes out and also can make liquid nitrogen enter rapidly the interior instant cooling sample of pipe.
Accompanying drawing explanation
Fig. 1 is bubble chamber schematic diagram in the utility model.
Fig. 2 is the utility model pilot scale tube fixing device schematic diagram.
Fig. 3 is centrifuge tube schematic diagram in the utility model.
In figure: 1, foam case lid; 2, bubble chamber; 3, test tube rack; 4, Guan Weikong; 5, Guan Gai; 6, centrifuge tube; 7, open-work (liquid nitrogen sees through).
Embodiment
The utility model is described further below in conjunction with accompanying drawing:
As shown in the figure: a kind of a small amount of fresh tobacco leaf metabolism group fast sampling sample freezing storage device, comprise that one is loaded with the bubble chamber 2 of liquid nitrogen and is arranged on the row of two in bubble chamber formula test tube rack 3, on test tube rack, be placed with the tip centrifuge tube 6 of a plurality of 50ml, each centrifuge tube be equipped with pipe cover 5 and centrifuge tube bottom be equipped with open-work 7, the height of open-work when the liquid level of liquid nitrogen is greater than centrifuge tube and is placed on test tube fixed mount.
The utility model operation instruction: before sampling, first by the centrifuge tube 6 of sample message or numbering mark, then centrifuge tube 6 is placed on the Guan Weikong 4 of test tube rack in bubble chamber 2, in bubble chamber 2, adds appropriate liquid nitrogen, liquid nitrogen liquid level will surpass open-work 7 positions of centrifuge tube 6; After sampling, with glass bar, sample is transferred in centrifuge tube 6, covered tightly pipe and cover 5, centrifuge tube after building, puts into other liquid nitrogen or dry ice chest is preserved, and then carries out the sampling of next group sample.
Claims (4)
1. an a small amount of fresh tobacco leaf metabolism group fast sampling sample freezing storage device, it is characterized in that: comprise that one is loaded with the bubble chamber of liquid nitrogen and is arranged on the test tube fixed mount in bubble chamber, on test tube fixed mount, be placed with the centrifuge tube of a plurality of 50ml, each centrifuge tube is equipped with pipe lid and centrifuge tube bottom is equipped with open-work, the height of open-work when the liquid level of liquid nitrogen is greater than centrifuge tube and is placed on test tube fixed mount.
2. a small amount of fresh tobacco leaf metabolism group fast sampling sample freezing storage device according to claim 1, is characterized in that: described test tube fixed mount is two row's formula test tube racks.
3. a small amount of fresh tobacco leaf metabolism group fast sampling sample freezing storage device according to claim 1, is characterized in that: described centrifuge tube is tip centrifuge tube.
4. a small amount of fresh tobacco leaf metabolism group fast sampling sample freezing storage device according to claim 1, is characterized in that: the open-work aperture on described centrifuge tube is 1-2mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420431713.9U CN204008269U (en) | 2014-08-01 | 2014-08-01 | A kind of a small amount of fresh tobacco leaf metabolism group fast sampling sample freezing storage device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420431713.9U CN204008269U (en) | 2014-08-01 | 2014-08-01 | A kind of a small amount of fresh tobacco leaf metabolism group fast sampling sample freezing storage device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204008269U true CN204008269U (en) | 2014-12-10 |
Family
ID=52048249
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420431713.9U Expired - Lifetime CN204008269U (en) | 2014-08-01 | 2014-08-01 | A kind of a small amount of fresh tobacco leaf metabolism group fast sampling sample freezing storage device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204008269U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104977179A (en) * | 2015-05-12 | 2015-10-14 | 青岛农业大学 | Novel portable biological tissue low-temperature freezing sampling tank |
| CN110537541A (en) * | 2019-08-20 | 2019-12-06 | 佛山科学技术学院 | Simple liquid nitrogen storage tank |
-
2014
- 2014-08-01 CN CN201420431713.9U patent/CN204008269U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104977179A (en) * | 2015-05-12 | 2015-10-14 | 青岛农业大学 | Novel portable biological tissue low-temperature freezing sampling tank |
| CN110537541A (en) * | 2019-08-20 | 2019-12-06 | 佛山科学技术学院 | Simple liquid nitrogen storage tank |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20141210 |
|
| CX01 | Expiry of patent term |