CN203949923U - A kind of rapid detection card that detects pig blue-ear disease poison antibody - Google Patents
A kind of rapid detection card that detects pig blue-ear disease poison antibody Download PDFInfo
- Publication number
- CN203949923U CN203949923U CN201320828453.4U CN201320828453U CN203949923U CN 203949923 U CN203949923 U CN 203949923U CN 201320828453 U CN201320828453 U CN 201320828453U CN 203949923 U CN203949923 U CN 203949923U
- Authority
- CN
- China
- Prior art keywords
- region
- ear disease
- disease poison
- pig blue
- nitrocellulose filter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 47
- 208000032625 disorder of ear Diseases 0.000 title claims abstract description 32
- 239000002574 poison Substances 0.000 title claims abstract description 30
- 231100000614 poison Toxicity 0.000 title claims abstract description 30
- 239000000020 Nitrocellulose Substances 0.000 claims abstract description 21
- 229920001220 nitrocellulos Polymers 0.000 claims abstract description 21
- 238000012360 testing method Methods 0.000 claims abstract description 13
- 230000000274 adsorptive effect Effects 0.000 claims abstract description 9
- 239000003365 glass fiber Substances 0.000 claims abstract description 9
- 238000012856 packing Methods 0.000 claims abstract description 4
- 239000000427 antigen Substances 0.000 claims description 5
- 102000036639 antigens Human genes 0.000 claims description 5
- 108091007433 antigens Proteins 0.000 claims description 5
- 239000000084 colloidal system Substances 0.000 claims description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 3
- 239000010931 gold Substances 0.000 claims description 3
- 229910052737 gold Inorganic materials 0.000 claims description 3
- 101710141454 Nucleoprotein Proteins 0.000 claims description 2
- 241000282898 Sus scrofa Species 0.000 description 24
- 208000024891 symptom Diseases 0.000 description 5
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 208000000059 Dyspnea Diseases 0.000 description 3
- 206010013975 Dyspnoeas Diseases 0.000 description 3
- 241000282887 Suidae Species 0.000 description 3
- 230000029058 respiratory gaseous exchange Effects 0.000 description 3
- 206010063409 Acarodermatitis Diseases 0.000 description 2
- 206010003591 Ataxia Diseases 0.000 description 2
- 208000003322 Coinfection Diseases 0.000 description 2
- 206010028470 Mycoplasma infections Diseases 0.000 description 2
- 208000005342 Porcine Reproductive and Respiratory Syndrome Diseases 0.000 description 2
- 241000447727 Scabies Species 0.000 description 2
- 206010000210 abortion Diseases 0.000 description 2
- 231100000176 abortion Toxicity 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 230000001684 chronic effect Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000000241 respiratory effect Effects 0.000 description 2
- 208000005687 scabies Diseases 0.000 description 2
- 208000011580 syndromic disease Diseases 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 208000012353 Contagious Pleuropneumonia Diseases 0.000 description 1
- 206010010947 Coordination abnormal Diseases 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 206010020843 Hyperthermia Diseases 0.000 description 1
- 241001062009 Indigofera Species 0.000 description 1
- 208000007542 Paresis Diseases 0.000 description 1
- 208000037581 Persistent Infection Diseases 0.000 description 1
- 241001135989 Porcine reproductive and respiratory syndrome virus Species 0.000 description 1
- 208000006399 Premature Obstetric Labor Diseases 0.000 description 1
- 206010036600 Premature labour Diseases 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 208000022531 anorexia Diseases 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 208000030303 breathing problems Diseases 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 206010061428 decreased appetite Diseases 0.000 description 1
- 230000000994 depressogenic effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000036031 hyperthermia Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 208000016290 incoordination Diseases 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 208000026475 palpebral edema Diseases 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 208000026440 premature labor Diseases 0.000 description 1
- 238000013102 re-test Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 208000012153 swine disease Diseases 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to a kind of rapid detection card that detects pig blue-ear disease poison antibody, comprises external packing box and test card, and described test card be oval, comprise get stuck and with support thin plate; Get stuck and be provided with well and judge view window; The inwall that supports thin plate comprises region one, region two, region three and region four, and region one and region three are downward-sloping from left to right, and described region two is horizontally disposed with, and described region four is inclined upwardly from left to right; Region two is provided with nitrocellulose filter, which is provided with detection line and control line; The left side in region one is provided with sample pad, and sample pad and nitrocellulose filter are connected by glass fibre element film, in region three, are provided with adsorptive pads, and adsorptive pads and described nitrocellulose filter connect.This rapid detection card is simple, convenient, quick, and result is clear easily to be distinguished, is applicable to peasant household and uses, and is easy to promote, and is suitable for the mass field fast detecting of peasant household's accident, and the Infect And Diagnose of pig blue-ear disease poison is played to booster action.
Description
Technical field
The utility model relates to food inspection field, is specifically related to a kind of rapid detection card that detects pig blue-ear disease poison antibody.
Background technology
Pig blue-ear disease, was once called as " mystery swine disease ", " new swine disease ", " pig Epidemic abortion and breathing syndrome ", " pig reproduction and breathing syndrome ", " swine fever epidemic disease " etc., and China is classified as two class infectious diseases.Pig blue-ear disease can be divided into acute, chronic type, Asia and faces the type of examining etc. facing to examine.
Acute: morbidity sow main manifestations be that spirit is depressed, poor appetite or useless absolutely, heating, appearance expiratory dyspnea in various degree, latter half of gestation (105～107 days), sow is miscarried, premature labor, stillborn foetus, mummy tire, weak son.Sow abortion ratio can reach 50%～70%, and mortinatality can reach more than 35%, and mummy can reach 25%, part newborn piglet performance expiratory dyspnea, and the symptoms such as ataxia and paresis, in 1 week postpartum, mortality ratio obviously increases (40%～80%).Minority sow shows as agalasisa in postpartum, afterbirth stagnates and vaginal fluid increases.1 the monthly age piglet show typical respiratory symptom, expiratory dyspnea, is abdominal respiration sometimes, anorexia or useless absolutely, more than hyperthermia to 40 DEG C, diarrhoea.Disorderly thick by hair, incoordination, gradual becoming thin, palpebral edema.The visible ear of small part piglet, skin surface are blue, and before wean, piglet mortality ratio can reach 80%～100%, and after wean, the weightening finish of piglet reduces, and daily gain can decline 50%～75%, mortality ratio raise (10%～25%).The resistance to pig poor growth of crossing, easily secondary other diseases.Growth pig and growing and fattening pigs show slight clinical symptom, have the Respiratory symptoms of distinct program, and minority case can show cough and darkviolet appears in the ears back side, edge, belly and tail skin.Easily there is scabies secondary infection in infected pigs, and occurs corresponding symptom.
Chronic type: this is the principal mode showing at large-scale pig farm PRRS at present.Main manifestations is that the production performance of swinery declines, poor growth, and sow group's reproductive performance declines, Swinery immunity function reduction, easily other bacillary and viral diseases of scabies secondary infection.Breathing problem (as mycoplasma infection, contagious pleuropneumonia, streptococcosis, the eperythrozoonosis) incidence of disease of swinery rises.
The type of examining is faced in Asia: infected pigs does not fall ill, and shows as the persistent infection of PRRSV, the serology antibody positive of swinery, and positive rate is generally 10%～88%.
And existing market relatively lacks a kind of easy to use, simple to operate, rapid detection card that this indigo plant otopathy also can detect in peasant household oneself.
Utility model content
The purpose of this utility model is to provide a kind of rapid detection card that detects pig blue-ear disease poison antibody, this rapid detection card, simple, convenient, quick, result is clear easily to be distinguished, be applicable to peasant household and use, be easy to promote, be suitable for the mass field fast detecting of peasant household's accident, be applicable to epidemiology survey, the Infect And Diagnose of pig blue-ear disease poison is played to booster action.
The purpose of this utility model is to be achieved through the following technical solutions:
A rapid detection card that detects pig blue-ear disease poison antibody, comprises the test card in external packing box and box, and described test card be ellipse, test card comprise oval-shaped get stuck with the oval-shaped support thin plate of the relative side that gets stuck; Described getting stuck is fixedly connected with the outer place that supports thin plate; Get stuck and be provided with well and judge view window; The inwall of described support thin plate comprises region one, region two, region three and region four, and described region one and region three are downward-sloping from left to right, and described region two is horizontally disposed with, and described region four is inclined upwardly from left to right; Region two is provided with nitrocellulose filter, on described nitrocellulose filter, be provided with successively from left to right detection line and control line, in described detection line, be coated with pig blue-ear disease poison antigen, be pig blue-ear disease poison N albumen, on described control line, be coated with anti-pig blue-ear disease poison antibody, i.e. the N protein polyclone antibody of anti-pig blue-ear disease poison; The left side in described region one is provided with sample pad, described sample pad and nitrocellulose filter are connected by glass fibre element film, in described glass fibre element film, be coated with the pig blue-ear disease poison antigen of dry colloid gold label, in described region three, be provided with adsorptive pads, described adsorptive pads and described nitrocellulose filter connect.
Further, described well is circular, describedly judges that view window is as rectangle or ellipse.
Further, described nitrocellulose filter is just to described judgement view window.
Further, described sample pad is just to described well.
The utility model provides a kind of rapid detection card that detects pig blue-ear disease poison antibody, and this rapid detection card mainly has following some beneficial effect:
1. this rapid detection card is simple, convenient, quick, does not need special instruments and equipment, does not need professional training;
2. this rapid detection card detection speed is fast, and result is clear, easily distinguish;
3. this rapid detection card is applicable to peasant household's use, is easy to promote, and is suitable for the mass field fast detecting of peasant household's accident, is applicable to epidemiology survey, and the Infect And Diagnose of pig blue-ear disease poison is played to booster action.
Brief description of the drawings
With reference to the accompanying drawings the utility model is described in further detail below.
Fig. 1 is the structural representation getting stuck in the rapid detection card of the detection pig blue-ear disease poison antibody described in the utility model embodiment;
Fig. 2 is the schematic diagram of nitrocellulose filter in the rapid detection card of detection pig blue-ear disease described in the utility model embodiment poison antibody;
Fig. 3 is the sectional view that supports thin plate in the rapid detection card of detection pig blue-ear disease described in the utility model embodiment poison antibody.
In figure:
1, get stuck; 2, support thin plate; 3, region one; 4, region two; 5, region three; 6, region four; 13, sample pad; 14, glass fibre element film; 15, nitrocellulose filter; 16, adsorptive pads; 23, well; 24, judge view window; 25, detection line; 26, control line.
Embodiment
As Figure 1-3, a kind of rapid detection card that detects pig blue-ear disease poison antibody described in the utility model embodiment, comprise the test card in external packing box and box, described test card is oval, test card comprise oval-shaped get stuck 1 with the oval-shaped support thin plate 2 of the relative side that gets stuck; Described get stuck 1 with support the outer place of thin plate 2 and be fixedly connected with; Get stuck and 1 be provided with well 23 and judge view window 24; The inwall of described support thin plate 2 comprises region 1, region 24, region 35 and region 46, and described region 1 and region 35 are downward-sloping from left to right, and described region 24 is horizontally disposed with, and described region 46 is inclined upwardly from left to right; Region 24 is provided with nitrocellulose filter 15, is provided with successively from left to right detection line 25 and control line 26 on described nitrocellulose filter 15, is coated with pig blue-ear disease poison antigen in described detection line 25, is coated with anti-pig blue-ear disease poison antibody on described control line 26; The left side in described region 1 is provided with sample pad 13, described sample pad 13 and nitrocellulose filter 15 are connected by glass fibre element film 14, in described glass fibre element film 14, be coated with the pig blue-ear disease poison antigen of dry colloid gold label, in described region 35, be provided with adsorptive pads 16, described adsorptive pads 16 connects with described nitrocellulose filter 15.
Further, described well 23 is circular, describedly judges that view window 24 is as rectangle or ellipse.Described nitrocellulose filter 15 is just to described judgement view window 24.Described sample pad 13 is just to described well 23.
When concrete use, test card is lain against on desktop, serum to be checked or blood sample are added to 2 (approximately 80 μ L) from well 23 with sample loading gun or syringe, sample arrives nitrocellulose filter 15 by sample pad 13 by glass fibre element film 14, and through detection line 25 and control line 26.After application of sample, start timing, result of determination at room temperature standing 5-20 minute, the result that exceedes 20 minutes can only be as a reference.Detect by an unaided eye and judge detection line 25 in view window 24 and the colour developing situation of control line 26, and judged result.There is a purplish red colo(u)r streak if only have in control line 26, negative; If in judgement view window 24, there is purplish red colo(u)r streak in detection line 25 and control line 26, positive simultaneously; Antibody titer in sample is higher, and detection line 25 colors are darker; If purplish red colo(u)r streak appears in detection line 25 and control line 26 simultaneously, but the very slight color that detection line 25 occurs is the weak positive; If detection line 25 and control line 26 do not occur purplish red colo(u)r streak or or only detection line 25 there is colo(u)r streak, it is invalid to represent, suggestion is used new test card by specification to require to retest.
The utility model is not limited to above-mentioned preferred forms; anyone can draw other various forms of products under enlightenment of the present utility model; no matter but do any variation in its shape or structure; every have identical with a application or akin technical scheme, within all dropping on protection domain of the present utility model.
Claims (5)
1. one kind is detected the rapid detection card of pig blue-ear disease poison antibody, comprise the test card in external packing box and box, it is characterized in that: described test card is for oval, test card comprise oval-shaped getting stuck (1) with the oval-shaped support thin plate (2) of the relative side that gets stuck; Described getting stuck (1) is fixedly connected with the outer place that supports thin plate (2); Get stuck (1) be provided with well (23) and judge view window (24); The inwall of described support thin plate (2) comprises region one (3), region two (4), region three (5) and region four (6), described region one (3) and region three (5) are downward-sloping from left to right, described region two (4) is horizontally disposed with, and described region four (6) is inclined upwardly from left to right; Region two (4) is provided with nitrocellulose filter (15), is provided with successively from left to right detection line (25) and control line (26) on described nitrocellulose filter (15); The left side in described region one (3) is provided with sample pad (13), described sample pad (13) and nitrocellulose filter (15) are connected by glass fibre element film (14), in described glass fibre element film (14), be coated with the pig blue-ear disease poison antigen of dry colloid gold label, in described region three (5), be provided with adsorptive pads (16), described adsorptive pads (16) connects with described nitrocellulose filter (15); Described well (23) is circular, describedly judges that view window (24) is as rectangle or ellipse.
2. the rapid detection card of detection pig blue-ear disease poison antibody according to claim 1, is characterized in that: described nitrocellulose filter (15) is just to described judgement view window (24).
3. the rapid detection card of detection pig blue-ear disease poison antibody according to claim 1, is characterized in that: described sample pad (13) is just to described well (23).
4. the rapid detection card of detection pig blue-ear disease poison antibody according to claim 1, is characterized in that: in described detection line (25), be coated with pig blue-ear disease poison N albumen.
5. the rapid detection card of detection pig blue-ear disease poison antibody according to claim 1, is characterized in that: the N protein polyclone antibody that is coated with anti-pig blue-ear disease poison on described control line (26).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320828453.4U CN203949923U (en) | 2013-12-17 | 2013-12-17 | A kind of rapid detection card that detects pig blue-ear disease poison antibody |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320828453.4U CN203949923U (en) | 2013-12-17 | 2013-12-17 | A kind of rapid detection card that detects pig blue-ear disease poison antibody |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203949923U true CN203949923U (en) | 2014-11-19 |
Family
ID=51891952
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320828453.4U Expired - Lifetime CN203949923U (en) | 2013-12-17 | 2013-12-17 | A kind of rapid detection card that detects pig blue-ear disease poison antibody |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203949923U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105974116A (en) * | 2016-07-12 | 2016-09-28 | 郑州中道生物技术有限公司 | Multi-linked rapid detection test strip for pig diseases and preparation method thereof |
| CN106771188A (en) * | 2016-12-07 | 2017-05-31 | 无锡艾科瑞思产品设计与研究有限公司 | A kind of rapid detection card for detecting pig blue-ear disease poison antibody |
| CN110894243A (en) * | 2019-12-16 | 2020-03-20 | 中国农业大学 | Porcine reproductive and respiratory syndrome virus chimeric antigen and colloidal gold immunochromatographic test strip for detecting porcine reproductive and respiratory syndrome virus antibody |
-
2013
- 2013-12-17 CN CN201320828453.4U patent/CN203949923U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105974116A (en) * | 2016-07-12 | 2016-09-28 | 郑州中道生物技术有限公司 | Multi-linked rapid detection test strip for pig diseases and preparation method thereof |
| CN106771188A (en) * | 2016-12-07 | 2017-05-31 | 无锡艾科瑞思产品设计与研究有限公司 | A kind of rapid detection card for detecting pig blue-ear disease poison antibody |
| CN110894243A (en) * | 2019-12-16 | 2020-03-20 | 中国农业大学 | Porcine reproductive and respiratory syndrome virus chimeric antigen and colloidal gold immunochromatographic test strip for detecting porcine reproductive and respiratory syndrome virus antibody |
| CN110894243B (en) * | 2019-12-16 | 2021-07-13 | 中国农业大学 | Porcine reproductive and respiratory syndrome virus chimeric antigen and colloidal gold immunochromatographic test strip for detecting porcine reproductive and respiratory syndrome virus antibody |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN205301333U (en) | Detection device is united to coxsackie virus A16 and enterovirus 71's igM antibody | |
| Casteels et al. | Neonatal screening for congenital cytomegalovirus infections | |
| FOX et al. | The Seattle virus watch: VII. Observations of adenovirus infections | |
| Kneyber et al. | Relationship between clinical severity of respiratory syncytial virus infection and subtype. | |
| CN203949923U (en) | A kind of rapid detection card that detects pig blue-ear disease poison antibody | |
| CN204666637U (en) | Mycoplasma pneumoniae IgM, IgG, IgA tri-test card | |
| CN103267844A (en) | Detection test paper strip of colloidal gold method for adenovirus IgM and IgG antibodies, kit and preparation method thereof | |
| CN209656716U (en) | A kind of 6 type of Coxsackie virus A and A10 type IgM antibody combined detection kit | |
| CN106168623B (en) | Respiratory Tract Adenovirus IgA antibody test strip and its detection method | |
| Menser et al. | Epidemiology of congenital rubella and results of rubella vaccination in Australia | |
| CN210347666U (en) | African swine fever antibody colloidal gold detection card | |
| CN106350607A (en) | Taqman real-time fluorescent PCR (Polymerase Chain Reaction) kit for detecting porcine epidemic diarrhea virus wild strains in porcine umbilical cord blood and use of PCR kit | |
| CN102445535A (en) | Contagious caprine plueropneumonia antibody detection test strips, and preparation method thereof | |
| CN203630140U (en) | Porcine virus antibody quintuple rapid detection card | |
| CN105954514A (en) | Bigeminal rapid detection test strip for foot-and-mouth disease and pseudorabies and preparation method thereof | |
| Chakraborty et al. | Secondary infection by anaerobic bacteria possibly ensues a battle for oxygen in SARS-Cov2 infected patients: anaerobe-targeting antibiotics (like doxycycline/Metronidazole) to supplement Azithromycin in the treatment regimen of COVID19? | |
| CN203465271U (en) | Rapid gold-labeled detection card for porcine pseudorabies antibody | |
| Hoyle et al. | Serological Diagnosis of Epidemic Influenza | |
| Tyrrell | Discovering and defining the etiology of acute respiratory viral disease | |
| CN204359792U (en) | Test strip and the kit of false negative result can be avoided | |
| FAWZY et al. | THE VIRUS WATCH PROGRAM: A CONTINUING SURVEILLANCE OF VIRAL INFECTION IN METROPOLITAN NEW YORK FAMILIES: V. OBSERVATIONS IN EMPLOYED ADULTS ON ETIOLOGY OF ACUTE UPPER RESPIRATORY DISEASE AND HETEROLOGOUS ANTIBODY RESPONSE TO RHINOVIRUSES | |
| CN204269653U (en) | Detect accurate fast gold mark test card | |
| McSherry | Why not prescribe antibiotics for" heavy colds'? | |
| CN213371886U (en) | Throat detector for pediatrics | |
| CN207928433U (en) | A kind of paper diaper showing infant urine situation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20141119 |
|
| CX01 | Expiry of patent term | ||
| DD01 | Delivery of document by public notice |
Addressee: SHENZHEN BAOANKANG BIOTECHNOLOGY Co.,Ltd. Person in charge of patents Document name: Notice of Termination of Patent Rights |
|
| DD01 | Delivery of document by public notice |