CN203935846U - A kind of Apparatus and system that is used to form concentration gradient - Google Patents
A kind of Apparatus and system that is used to form concentration gradient Download PDFInfo
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- CN203935846U CN203935846U CN201420317194.3U CN201420317194U CN203935846U CN 203935846 U CN203935846 U CN 203935846U CN 201420317194 U CN201420317194 U CN 201420317194U CN 203935846 U CN203935846 U CN 203935846U
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- concentration
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- concentration gradient
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- 239000007853 buffer solution Substances 0.000 claims abstract description 48
- 239000012452 mother liquor Substances 0.000 claims abstract description 44
- 239000007788 liquid Substances 0.000 claims abstract description 31
- 238000002347 injection Methods 0.000 claims abstract description 11
- 239000007924 injection Substances 0.000 claims abstract description 11
- 238000006243 chemical reaction Methods 0.000 claims abstract description 9
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 7
- 239000000758 substrate Substances 0.000 claims description 8
- 239000000376 reactant Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 description 6
- 238000009792 diffusion process Methods 0.000 description 5
- 239000004205 dimethyl polysiloxane Substances 0.000 description 5
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 5
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 5
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 5
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000872 buffer Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000007877 drug screening Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000036457 multidrug resistance Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 229920005573 silicon-containing polymer Polymers 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
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- Physical Or Chemical Processes And Apparatus (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The utility model discloses a kind of Apparatus and system that is used to form concentration gradient.Described device comprises that many group concentration forms passage, described passage one end is outlet, other end injection port, described concentration forms passage and to the direction that goes out sample, is laid with successively hybrid channel and reaction chamber according to sample introduction, every group of concentration forms its injection port of passage and is connected with buffer solution liquid storage tank with mother liquor liquid storage tank respectively with buffer solution split channel by mother liquor split channel, every group of concentration forms passage, different according to the aimed concn that will form, its mother liquor split channel is different with the length ratio of buffer solution split channel.Described system comprises that described concentration gradient forms device and negative pressure shape apparatus for converting, and described concentration gradient forms all concentration in device and forms being connected of its outlets of passage and negative pressure shape apparatus for converting, and the other end of described concentration gradient formation device is under same pressure.Described Apparatus and system, compact conformation, can once form multiple concentration, and stability is high, applied range.
Description
Technical field
The utility model belongs to biology device field, more specifically, relates to a kind of Apparatus and system that is used to form concentration gradient.
Background technology
Concentration gradient forms device, can realize the formation of the drug sample of variable concentrations gradient, and institute's amount of reagent that consumes is to receive upgrading other, is considered to high flux and studies cytositimulation, an effective tool of drug screening.
Researchers have set up multiple microfluidic concentration gradient device by injecting two kinds of different liquid for high throughput analysis, mainly comprise that the concentration gradient based on molecular diffusion forms device and forms device based on repeatedly shunting the concentration gradient of collaborating.Concentration gradient based on molecular diffusion forms device, as the concentration gradient chip based on molecular diffusion, simple to operate, process simple and easy, but due to the systematic error of diffusion, this chip is difficult to form perfect linear pattern concentration gradient, the more important thing is that this chip has been subject to molecular size greatly, flow velocity, the impact of diffusion length and liquid viscosity.Concentration gradient based on repeatedly shunting is collaborated forms device, as the concentration gradient chip based on shunting interflow, by chip structure, operate multichannel liquid stream and form concentration gradient, often structure is too complicated, in the process of collaborating in shunting each time, need straight channel or the complicated micro-mixer grown to realize, and the complexity of its structure increases along with increasing of concentration, greatly strengthened the redundancy of concentration gradient chip formation concentration gradient.
Utility model content
Above defect or Improvement requirement for prior art, the utility model provide a kind of simple in structure, process simple and easy, good stability concentration gradient form micro fluidic device, its object is to overcome existing concentration gradient and forms micro fluidic device complex structure, the shortcoming of poor stability, solves the technical problem of the experiments such as high-flux medicaments sifting thus.
For achieving the above object, according to an aspect of the present utility model, a kind of device that is used to form concentration gradient is provided, in horizontal substrate, comprise that many group concentration forms passage, described passage one end is outlet, other end injection port, described concentration forms passage and to the direction that goes out sample, is laid with successively hybrid channel and reaction chamber according to sample introduction, every group of concentration forms its injection port of passage and is connected with buffer solution liquid storage tank with mother liquor liquid storage tank respectively with buffer solution split channel by mother liquor split channel, every group of concentration forms passage, different according to the aimed concn that will form, its mother liquor split channel is different with the length ratio of buffer solution split channel,
During work, control described concentration formation passage pressure at two ends poor identical, mother liquor forms according to every group of concentration mother liquor split channel and the buffer solution split channel length that passage is connected with buffer solution, with different ratios, enter corresponding concentration and form passage, during through its hybrid channel, mother liquor and buffer solution evenly mix, and form the reactant liquor of variable concentrations, are stored in reaction chamber.
Preferably, the described device that is used to form concentration gradient, its mother liquor split channel and buffer solution split channel cross-sectional width and highly identical, the length ratio of its mother liquor split channel and buffer solution split channel is inversely proportional to definite according to mother liquid flow rate and buffer solution flow proportional and its respective channel length.
Preferably, described in be used to form the device of concentration gradient, its many group concentration forms passages and shares same outlets.
Preferably, described in be used to form the device of concentration gradient, its many group concentration forms passages centered by outlet, evenly arranged radially.
Preferably, described in be used to form the device of concentration gradient, during work, described outlet forms negative pressure.
Preferably, described in be used to form the device of concentration gradient, its hybrid channel is S type serpentine channel.
According on the other hand of the present utility model, a kind of system that is used to form concentration gradient is provided, comprise the described device that is used to form concentration gradient and negative pressure shape apparatus for converting, in the described device that is used to form concentration gradient, all concentration forms its outlets of passage and is connected with negative pressure shape apparatus for converting, described in be used to form concentration gradient the other end of device under same pressure.
In general, the above technical scheme of conceiving by the utility model compared with prior art, can obtain following beneficial effect:
(1) owing to adopting mother liquor split channel and the buffer solution split channel of different length ratio, control the ratio of mother liquor and buffer solution, therefore can form easily the goal response liquid of various concentration, do not need to form respective concentration by complicated pipe-line system;
(2) compare with traditional chip form, the concentration ratio after liquid diluting is determined by chip structure completely, has greatly improved the stability of this chip;
(3) concentration that the utility model is conceived forms device, and compact conformation is applicable to small-sized or miniature concentration and forms device, is widely used;
(4) mode of described device disposable interflow dilution realizes the dilution of many concentration, and this method forms chip with the concentration gradient at multistep shunting interflow and compares, and structure is more simple, and redundancy is lower, need to form the micro-mixer of steady concentration still less.This device provides a kind of new approach for research comprises the experiments such as cell multidrug resistance, multiple, based on fields such as cell experiment and biochemical reactions, is with a wide range of applications;
(5) system that is used to form concentration gradient described in realizes system sample introduction by a road negative pressure and multi-path fluid is controlled, compare with traditional two-way input mode, the flow velocity of liquid be can't help the two-way flow rate of liquid impact of sample introduction, the unstable pulse that pump and syringe cause does not affect the dilution of liquid, has greatly improved stability.
Accompanying drawing explanation
Fig. 1 is the apparatus structure schematic diagram that is used to form concentration gradient in embodiment 1;
Fig. 2 is the system architecture schematic diagram that is used to form concentration gradient in embodiment 2.
In institute's drawings attached, identical Reference numeral is used for representing identical element or structure, wherein: 1-8 is divided into concentration and forms passage, 11 is outlet, and 12 is injection port, and 13 is hybrid channel, 14 is reaction chamber, and 15 is mother liquor split channel, and 16 is buffer solution split channel, 17 is mother liquor liquid storage tank, and 18 is buffer solution liquid storage tank, and 20 for being used to form the device of concentration gradient, 21 is Micropump, and 22 is sensor, and 23 is hand-operated valve, 24 is triple valve, and 25 is gas cylinder, and 26 is switch.
The specific embodiment
In order to make the purpose of this utility model, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the utility model is further elaborated.Should be appreciated that specific embodiment described herein is only in order to explain the utility model, and be not used in restriction the utility model.In addition,, in each embodiment of described the utility model, involved technical characterictic just can not combine mutually as long as do not form each other conflict.
The device that is used to form concentration gradient that the utility model provides, in horizontal substrate, comprise that many group concentration forms passage, described passage one end is outlet 11, other end injection port 12, described concentration forms passage and to the direction that goes out sample, is laid with successively 13He hybrid channel, hybrid channel 13 according to sample introduction, every group of concentration forms its injection port 12 of passage and is connected with buffer solution liquid storage tank 18 with mother liquor liquid storage tank 17 respectively with buffer solution split channel 16 by mother liquor split channel 15, every group of concentration forms passage, different according to the aimed concn that will form, its mother liquor split channel 15 is different with the length ratio of buffer solution split channel 16.
When mother liquor split channel 15 and buffer solution split channel 16 cross-sectional width with when highly identical, the length ratio of its mother liquor split channel 15 and buffer solution split channel 16 is inversely proportional to definite according to mother liquid flow rate and buffer solution ratio and its respective channel length,
L wherein
afor mother liquor split channel 15 length, L
bbuffer solution split channel 16 length, Q
afor mother liquid flow rate, Q
bfor buffer solution flow, the aimed concn that mother liquor and buffer solution form after evenly mixing is:
Wherein, C
expectedfor target solution concentration, Q
aand Q
bbe respectively mother liquid flow rate and buffering flow quantity, C
aand C
bbe respectively mother liquid concentration and buffer concentration, L
aand L
bbe respectively mother liquor split channel 15 length and buffer solution split channel 16 length.
Preferably, described many group concentration forms passage and shares same outlet 11, organizes concentration more and forms passage centered by outlet 11, evenly arranged radially.
Described hybrid channel 13, is preferably S type serpentine channel.
During work, control described concentration formation passage pressure at two ends poor identical, mother liquor forms according to every group of concentration mother liquor split channel 15 and buffer solution split channel 16 length that passage is connected with buffer solution, with different ratios, enter corresponding concentration and form passage, during through its hybrid channel 13, mother liquor and buffer solution evenly mix, and form the reactant liquor of variable concentrations, are stored in hybrid channel 13.
When described many group concentration forms the shared same outlet 11 of passage, at outlet 11, form negative pressure, and mother liquor liquid storage tank 17 and buffer solution liquid storage tank 18Yu air UNICOM, said many group concentration forms passage pressure at two ends and remains identical pressure differential.
The system that is used to form concentration gradient that the utility model provides, comprise described device and the negative pressure shape apparatus for converting forming for concentration gradient, in the described device that is used to form concentration gradient, all concentration forms its outlets 11 of passage and is connected with negative pressure shape apparatus for converting, described in be used to form concentration gradient the other end of device under same pressure.
Be below embodiment:
Embodiment 1
Being used to form a device for concentration gradient, is micro-fluidic chip, by dimethyl silicone polymer thin layer and substrate bonding, is formed.PDMS thin layer is provided with 8 groups of concentration and forms passage, as shown in Figure 1, in the substrate of level, comprise that 8 groups of concentration form passage, described passage one end is outlet 11, other end injection port 12,8 groups of concentration form passage and share same outlet 11, organize concentration more and form passage centered by outlet 11, evenly arranged radially.Described concentration forms passage and to the direction that goes out sample, is laid with successively hybrid channel 13 and reaction chamber 14 according to sample introduction, and every group of concentration forms its injection port 12 of passage and is connected with buffer solution liquid storage tank 17 with mother liquor liquid storage tank 17 respectively with buffer solution split channel 16 by mother liquor split channel 15.Described hybrid channel 13 is S type serpentine channel.
Every group of concentration forms passage, and different according to the aimed concn that will form, its mother liquor split channel 15 is different with the length ratio of buffer solution split channel 16.Mother liquor split channel 15 and buffer solution split channel 16 cross-sectional width and highly identical, width is 200 microns, is highly 50 microns.Each organizes the length of mother liquor split channel 15 and buffer solution split channel 16, as shown in table 1.
During work, at outlet 11, form negative pressure, range of negative pressure at 0.8kPa between 4kPa, and mother liquor liquid storage tank 17 and buffer solution liquid storage tank 17Yu air UNICOM, said many group concentration forms passage pressure at two ends and remains identical pressure differential.Mother liquor forms according to every group of concentration mother liquor split channel 15 and buffer solution split channel 16 length that passage is connected with buffer solution, with different ratios, enter corresponding concentration and form passage, during through its hybrid channel 13, mother liquor and buffer solution evenly mix, form the reactant liquor of variable concentrations, be stored in reaction chamber 14, in Table 1.
Table 1 concentration forms the aimed concn of passage
Note: wherein, take mother liquid concentration as 1, buffer concentration is 0.
The detailed process of the bonding of PDMS and substrate is as follows: the PDMS thin layer that contains microchannel and the clean substrate that contains sample holes are placed in to plasma clean device and process (800V, 2min), at bonding face, drip a small amount of ultra-pure water, then under vertical microscope, three sample holes on PDMS thin layer are accurately aimed at three holes on glass substrate, then be placed in vacuum drying oven, under vacuum state, 65 ℃ of heating obtained PDMS chip after 2 hours.
Embodiment 2
A kind of system that is used to form concentration gradient, as shown in Figure 2, comprise as the device and the negative pressure shape apparatus for converting that form for concentration gradient in embodiment 1, in the described device that is used to form concentration gradient, all concentration forms its outlets 11 of passage and is connected with negative pressure shape apparatus for converting, described in be used to form concentration gradient the other end of device under same pressure.
Described negative pressure shape apparatus for converting comprises Micropump 21, sensor 22, hand-operated valve 23, triple valve 24 and gas cylinder 25.Gas cylinder internal is inserted in sensor one end, monitors in real time gas pressure in gas cylinder, and other end connecting valve 26 is by institute's monitoring pressure value feedback; 21 1 sections of connection gas cylinders of Micropump, for regulating gas cylinder internal pressure, other end connecting valve 26 is controlled its states; Hand-operated valve 23 is connected to gas cylinder 25, for manual releasing gas cylinder internal pressure; Triple valve 24 one end connect gas cylinder, one end connects the outlet 11 of the device 20 forming for concentration gradient in embodiment 1, last port is connected with atmosphere, during unlatching, connect described device 20 and the gas cylinder forming for concentration gradient, while closing, connect described device 20 and the atmosphere forming for concentration gradient.
During work, when being used to form outlet 11 and the gas cylinder of the device 20 of concentration gradient described in triple valve 24 unlatching connections, hand-operated valve 23 cuts out, switch 26 is opened, the described device forming for concentration gradient 20 outlets 11 form negative pressure, thereby at the reactant liquor of the interior formation aimed concn of individual reaction chamber 14.
Those skilled in the art will readily understand; the foregoing is only preferred embodiment of the present utility model; not in order to limit the utility model; all any modifications of doing within spirit of the present utility model and principle, be equal to and replace and improvement etc., within all should being included in protection domain of the present utility model.
Claims (7)
1. a device that is used to form concentration gradient, it is characterized in that, in horizontal substrate, comprise that many group concentration forms passage, described passage one end is outlet, other end injection port, described concentration forms passage and to the direction that goes out sample, is laid with successively hybrid channel and reaction chamber according to sample introduction, every group of concentration forms its injection port of passage and is connected with buffer solution liquid storage tank with mother liquor liquid storage tank respectively with buffer solution split channel by mother liquor split channel, every group of concentration forms passage, different according to the aimed concn that will form, its mother liquor split channel is different with the length ratio of buffer solution split channel,
During work, control described concentration formation passage pressure at two ends poor identical, mother liquor forms according to every group of concentration mother liquor split channel and the buffer solution split channel length that passage is connected with buffer solution, with different ratios, enter corresponding concentration and form passage, during through its hybrid channel, mother liquor and buffer solution evenly mix, and form the reactant liquor of variable concentrations, are stored in reaction chamber.
2. the device that is used to form concentration gradient as claimed in claim 1, it is characterized in that, mother liquor split channel and buffer solution split channel cross-sectional width and highly identical, the length ratio of its mother liquor split channel and buffer solution split channel is inversely proportional to definite according to mother liquid flow rate and buffer solution flow proportional and its respective channel length.
3. the device that is used to form concentration gradient as claimed in claim 1, is characterized in that, described many group concentration forms passage and shares same outlet.
4. the device that is used to form concentration gradient as claimed in claim 3, is characterized in that, described many group concentration forms passage centered by outlet, evenly arranged radially.
5. the device that is used to form concentration gradient as claimed in claim 3, is characterized in that, during work, described outlet forms negative pressure.
6. the device that is used to form concentration gradient as claimed in claim 1, is characterized in that, described hybrid channel is S type serpentine channel.
7. a system that is used to form concentration gradient, it is characterized in that, comprise the device that is used to form concentration gradient and negative pressure shape apparatus for converting as described in claim 1 to 4 any one, in the described device that is used to form concentration gradient, all concentration forms its outlets of passage and is connected with negative pressure shape apparatus for converting, described in be used to form concentration gradient the other end of device under same pressure.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201420317194.3U CN203935846U (en) | 2014-06-13 | 2014-06-13 | A kind of Apparatus and system that is used to form concentration gradient |
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| CN201420317194.3U CN203935846U (en) | 2014-06-13 | 2014-06-13 | A kind of Apparatus and system that is used to form concentration gradient |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104084245A (en) * | 2014-06-13 | 2014-10-08 | 华中科技大学 | Apparatus and system used for forming concentration gradient |
| CN105482989A (en) * | 2016-01-19 | 2016-04-13 | 广州迪澳生物科技有限公司 | Multi-index detecting micro-fluidic chip and detection method |
| CN109991035A (en) * | 2017-12-29 | 2019-07-09 | 台达电子工业股份有限公司 | Trace sampling apparatus |
| CN110193384A (en) * | 2018-07-24 | 2019-09-03 | 中国科学院武汉物理与数学研究所 | It is a kind of for the three dimensional concentration gradient array device of biochemical reaction conditional filtering and application |
-
2014
- 2014-06-13 CN CN201420317194.3U patent/CN203935846U/en not_active Expired - Lifetime
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104084245A (en) * | 2014-06-13 | 2014-10-08 | 华中科技大学 | Apparatus and system used for forming concentration gradient |
| CN105482989A (en) * | 2016-01-19 | 2016-04-13 | 广州迪澳生物科技有限公司 | Multi-index detecting micro-fluidic chip and detection method |
| CN105482989B (en) * | 2016-01-19 | 2018-06-29 | 广州迪澳生物科技有限公司 | A kind of multiple determination micro-fluidic chip and detection method |
| CN109991035A (en) * | 2017-12-29 | 2019-07-09 | 台达电子工业股份有限公司 | Trace sampling apparatus |
| CN109991035B (en) * | 2017-12-29 | 2021-12-24 | 台达电子工业股份有限公司 | Micro-sampling device |
| CN110193384A (en) * | 2018-07-24 | 2019-09-03 | 中国科学院武汉物理与数学研究所 | It is a kind of for the three dimensional concentration gradient array device of biochemical reaction conditional filtering and application |
| CN110193384B (en) * | 2018-07-24 | 2020-06-19 | 中国科学院武汉物理与数学研究所 | Three-dimensional concentration gradient array device for biochemical reaction condition screening and application |
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Effective date of registration: 20230119 Address after: 101, Floor 1, Building 1, No. 8, Heiquan Road, Haidian District, Beijing, 100192 Patentee after: Black Jade Star Rock International Science and Technology (Beijing) Co.,Ltd. Address before: 430074 Hubei Province, Wuhan city Hongshan District Luoyu Road No. 1037 Patentee before: HUAZHONG University OF SCIENCE AND TECHNOLOGY |