CN203923069U - A kind of complexes of collagen protein purifying - Google Patents
A kind of complexes of collagen protein purifying Download PDFInfo
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- CN203923069U CN203923069U CN201420324710.5U CN201420324710U CN203923069U CN 203923069 U CN203923069 U CN 203923069U CN 201420324710 U CN201420324710 U CN 201420324710U CN 203923069 U CN203923069 U CN 203923069U
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Abstract
The utility model provides a kind of complexes of collagen protein purifying, comprise the dissolving vessel, storage tank, treatment tank and the cooling driers that utilize pipeline to connect successively, in described dissolving vessel, be provided with stirring rake, in the pipeline of described connection dissolving vessel and storage tank, be provided with pump and whizzer; In the pipeline of described connection storage tank and treatment tank, be provided with pump and separator column, described separator column is provided with the film that filters crude extract; Described separator column top is provided with the pipeline of introducing damping fluid, and the pipeline of described introducing damping fluid is provided with pump; Described treatment tank is connected with ultrafiltration system circulation by pipeline, in the pipeline of described connection treatment tank and ultrafiltration system, is provided with pump; Described treatment tank is provided with the pipeline of introducing distilled water, in described pipeline, is provided with pump.The utility model has improved the percentage extraction of collagen protein, has reduced pollution thereby be difficult for bringing miscellaneous bacteria in preparation process, has improved the quality of product.
Description
Technical field
The utility model relates to a kind of complexes of collagen protein purifying.
Background technology
Collagen protein is the biomaterial being most widely used in the world, and International Year output is 20,000 tons of left and right at present.At medical field, collagen protein is widely used in wound control and transforms medical science as high-biocompatibility, high biological activity material.2011, the market study data of the internationally famous commercial affairs test and appraisal Frost & Sullivan of mechanism also show, collagen protein has become the biomedical material that has the market competitiveness most, for regenerative medicine, and will further develop, becoming one of organ alternative medicine two large materials, the ready level of its technology---Technology readiness level (TRL) scoring is 8 (the highest by 9).Medicine food management board of the U.S. (FDA) prediction, will occupy the biomedical material market that exceedes 20% to collagen protein in 2015, and annual sales amount exceedes 20,000,000,000 dollars.
All the time, collagen protein mainly extracts from the histoorgan of animal, as the skin of pig ox, bone and muscle tendon etc.In recent years, international large-lot producer takes up to develop the research and development of synthetic collagen protein and analogue thereof, but limited by synthetic technology, level is lower, output is limited, and expensive (price of market synthesis of high purity collagen protein is up to 3000 dollars/milligram at present), only can be used for research application.
In the market of collagen protein application aspect, international well-known medicine equipment enterprise, all in the research and development of carrying out collagen protein medical use, comprises wound wrapper material, special open sore part control creme, collagen protein foam and sponge etc.Taking collagen product as substrate material, add cell and even stem cells technology, develop the organ transplantation of new cytoactive and organ and repair product and become the new technological trend of industry.
At present, the still acid hydrolyzation based on traditional of the modal collagen protein extraction process of China, and also main raw material is Cowhells tendon (tendons of beef, mutton or pork) and mouse tail muscle tendon.Due to technology limitation, acid hydrolyzation extraction efficiency can only calculate from mouse tail muscle tendon weight in wet base, and productive rate is no more than 60%.In order to improve percentage extraction, in a lot of situations, also with trypsinase, muscle tendon is carried out to pre-treatment.Although it is collagen protein that animal muscle tendon exceedes 90% component, its stripping process manpower consumption from animal body is large, and is difficult for processing clean, causes the after stain of product assorted, and particularly the pollution of animal blood and muscle tissue is difficult to remove.In addition, the proteolytic enzyme of a lot of animal tissuess still exists, in acidolysis process, meeting and acid react on collagen protein simultaneously, cause the irregular hydrolysis of collagen protein, greatly reduce the controllability of flow process with repeatable, directly have influence on the quality, particularly its special biological function of collagen protein.Therefore, need to design a kind of purifying suite of equipment and extract highly purified collagen protein.
Utility model content
The utility model, in order to overcome the deficiencies in the prior art, provides a kind of complexes of collagen protein purifying, has improved the percentage extraction of collagen protein, has reduced pollution thereby be difficult for bringing miscellaneous bacteria in preparation process, has improved the quality of product.
For achieving the above object, the utility model provides a kind of complexes of collagen protein purifying, comprise the dissolving vessel, storage tank, treatment tank and the cooling driers that utilize pipeline to connect successively, in described dissolving vessel, be provided with stirring rake, in the pipeline of described connection dissolving vessel and storage tank, be provided with pump and whizzer; In the pipeline of described connection storage tank and treatment tank, be provided with pump and separator column, described separator column is provided with the film that filters crude extract; Described separator column top is provided with the pipeline of introducing damping fluid, and the pipeline of described introducing damping fluid is provided with pump; Described treatment tank is connected with ultrafiltration system circulation by pipeline, in the pipeline of described connection treatment tank and ultrafiltration system, is provided with pump; Described treatment tank is provided with the pipeline of introducing distilled water, in described pipeline, is provided with pump.
Further, described ultrafiltration system top and the bottom are provided with the pipeline of getting rid of filtrate, and filtrate is discharged to collected outside device by pipeline.
Further, in described separator column and refining tank connected pipeline, be provided with UV-detector, detect filtrate ultraviolet absorption value.
Owing to having adopted technique scheme, the beneficial effects of the utility model are:
The complexes of a kind of collagen protein purifying that the utility model provides, comprise the dissolving vessel, storage tank, treatment tank and the cooling driers that connect successively with pipeline, improve the percentage extraction of collagen protein, reduced pollution thereby be difficult for bringing miscellaneous bacteria in preparation process, improved the quality of product.
Brief description of the drawings
Below in conjunction with drawings and Examples, the utility model is described in further detail.
Fig. 1 structural representation of the present utility model;
In figure: 1-dissolving vessel, 2-storage tank, 3-treatment tank, 4-cooling driers, 5-stirring rake, 6-whizzer, 7-separator column, 8-film, 9-ultrafiltration system, 10-UV-detector.
Embodiment
By reference to the accompanying drawings the utility model is described in further detail now.These accompanying drawings are the schematic diagram of simplification, and basic structure of the present utility model is only described in a schematic way, and therefore it only shows the formation relevant with the utility model.
As shown in Figure 1, the utility model provides a kind of complexes of collagen protein purifying, comprise the dissolving vessel 1, storage tank 2, treatment tank 3 and the cooling driers 4 that utilize pipeline to connect successively, it is characterized in that, in described dissolving vessel 1, be provided with stirring rake 5, in the pipeline of described connection dissolving vessel 1 and storage tank 2, be provided with pump and whizzer 6; In the pipeline of described connection storage tank 2 and treatment tank 3, be provided with pump and separator column 7, described separator column 7 is provided with the film 8 that filters crude extract; Described separator column 7 tops are provided with the pipeline of introducing damping fluid, and the pipeline of described introducing damping fluid is provided with pump; Described treatment tank 3 is connected with ultrafiltration system 9 circulations by pipeline, in the pipeline of described connection treatment tank 3 and ultrafiltration system 9, is provided with pump; On described treatment tank, 3 are provided with the pipeline of introducing distilled water, in described pipeline, are provided with pump.
As preferred version of the present utility model, described ultrafiltration system 9 top and the bottom are provided with the pipeline of getting rid of filtrate, and filtrate is discharged to collected outside device by pipeline.
As preferred version of the present utility model, in the pipeline that described separator column 7 is connected with treatment tank 3, be provided with UV-detector 10, detect filtrate ultraviolet absorption value, determine and collect refining protein liquid.
Principle of work of the present utility model is as follows: the tail tendon of peeling off through physical method, through cleaning after fragmentation, is fed intake and enters in dissolving vessel 1, after stirring rake stirs for 5 one days above, use pump to be dosed in whizzer 6, after centrifugal, obtain crude extract, in storage tank 2, preserve; Then pumped in separator column 7 by pump, pumping into after a certain amount of volumes of filtrate, open pump, pump into damping fluid, power is provided, protein crude extract is after film 8 filters, carry out gradient separations, detect filtrate ultraviolet absorption value by UV-detector 10, determine and collect refining protein liquid, will refine protein liquid and preserve in treatment tank 3; To refine protein liquid by pump and pump into ultrafiltration system 9, and be back in treatment tank 3 by pipeline, filtrate is got rid of by pipeline, synchronously adds distilled water according to leaching liquid measure by pump; Through 5-10 doubly continuously after filter wash, will refine protein liquid charging and put into cooling driers 4 and carry out freeze-drying.
Claims (3)
1. complexes for collagen protein purifying, comprise the dissolving vessel, storage tank, treatment tank and the cooling driers that utilize pipeline to connect successively, it is characterized in that, in described dissolving vessel, are provided with stirring rake, in the pipeline of described connection dissolving vessel and storage tank, are provided with pump and whizzer; In the pipeline of described connection storage tank and treatment tank, be provided with pump and separator column, described separator column is provided with the film that filters crude extract; Described separator column top is provided with the pipeline of introducing damping fluid, and the pipeline of described introducing damping fluid is provided with pump; Described treatment tank is connected with ultrafiltration system circulation by pipeline, in the pipeline of described connection treatment tank and ultrafiltration system, is provided with pump; Described treatment tank is provided with the pipeline of introducing distilled water, in described pipeline, is provided with pump.
2. the complexes of a kind of collagen protein purifying according to claim 1, is characterized in that, described ultrafiltration system top and the bottom are provided with the pipeline of getting rid of filtrate.
3. the complexes of a kind of collagen protein purifying according to claim 1 and 2, is characterized in that, in described separator column and refining tank connected pipeline, are provided with UV-detector.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420324710.5U CN203923069U (en) | 2014-06-18 | 2014-06-18 | A kind of complexes of collagen protein purifying |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420324710.5U CN203923069U (en) | 2014-06-18 | 2014-06-18 | A kind of complexes of collagen protein purifying |
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| CN203923069U true CN203923069U (en) | 2014-11-05 |
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| CN201420324710.5U Active CN203923069U (en) | 2014-06-18 | 2014-06-18 | A kind of complexes of collagen protein purifying |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104017072A (en) * | 2014-06-18 | 2014-09-03 | 常州药物研究所有限公司 | Complete device for collagen purification |
| CN106994261A (en) * | 2017-05-17 | 2017-08-01 | 连云港金喜食品有限公司 | Purification devices |
-
2014
- 2014-06-18 CN CN201420324710.5U patent/CN203923069U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104017072A (en) * | 2014-06-18 | 2014-09-03 | 常州药物研究所有限公司 | Complete device for collagen purification |
| CN106994261A (en) * | 2017-05-17 | 2017-08-01 | 连云港金喜食品有限公司 | Purification devices |
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