CN203838157U - Combined detection card for aflatoxin B1 and deoxynivalenol - Google Patents
Combined detection card for aflatoxin B1 and deoxynivalenol Download PDFInfo
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- CN203838157U CN203838157U CN201420082102.8U CN201420082102U CN203838157U CN 203838157 U CN203838157 U CN 203838157U CN 201420082102 U CN201420082102 U CN 201420082102U CN 203838157 U CN203838157 U CN 203838157U
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- aflatoxin
- vomitoxin
- detection line
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- gold
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Links
- 238000001514 detection method Methods 0.000 title claims abstract description 60
- LINOMUASTDIRTM-QGRHZQQGSA-N deoxynivalenol Chemical compound C([C@@]12[C@@]3(C[C@@H](O)[C@H]1O[C@@H]1C=C(C([C@@H](O)[C@@]13CO)=O)C)C)O2 LINOMUASTDIRTM-QGRHZQQGSA-N 0.000 title claims abstract description 49
- LINOMUASTDIRTM-UHFFFAOYSA-N vomitoxin hydrate Natural products OCC12C(O)C(=O)C(C)=CC1OC1C(O)CC2(C)C11CO1 LINOMUASTDIRTM-UHFFFAOYSA-N 0.000 title claims abstract description 49
- OQIQSTLJSLGHID-WNWIJWBNSA-N aflatoxin B1 Chemical compound C=1([C@@H]2C=CO[C@@H]2OC=1C=C(C1=2)OC)C=2OC(=O)C2=C1CCC2=O OQIQSTLJSLGHID-WNWIJWBNSA-N 0.000 title claims abstract description 42
- 239000002115 aflatoxin B1 Substances 0.000 title claims abstract description 41
- 229930020125 aflatoxin-B1 Natural products 0.000 title claims abstract description 41
- 229930002954 deoxynivalenol Natural products 0.000 title abstract description 7
- 238000012360 testing method Methods 0.000 claims abstract description 33
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims abstract description 19
- 238000006243 chemical reaction Methods 0.000 claims abstract description 17
- 239000004033 plastic Substances 0.000 claims abstract description 15
- 229920003023 plastic Polymers 0.000 claims abstract description 15
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- 229910052737 gold Inorganic materials 0.000 claims abstract description 7
- 230000027455 binding Effects 0.000 claims description 16
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- 231100000678 Mycotoxin Toxicity 0.000 description 4
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- MBMQEIFVQACCCH-UHFFFAOYSA-N trans-Zearalenon Natural products O=C1OC(C)CCCC(=O)CCCC=CC2=CC(O)=CC(O)=C21 MBMQEIFVQACCCH-UHFFFAOYSA-N 0.000 description 4
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- 241000223194 Fusarium culmorum Species 0.000 description 2
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- VYLQGYLYRQKMFU-UHFFFAOYSA-N Ochratoxin A Natural products CC1Cc2c(Cl)cc(CNC(Cc3ccccc3)C(=O)O)cc2C(=O)O1 VYLQGYLYRQKMFU-UHFFFAOYSA-N 0.000 description 2
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- DAEYIVCTQUFNTM-UHFFFAOYSA-N ochratoxin B Natural products OC1=C2C(=O)OC(C)CC2=CC=C1C(=O)NC(C(O)=O)CC1=CC=CC=C1 DAEYIVCTQUFNTM-UHFFFAOYSA-N 0.000 description 2
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- 230000001079 digestive effect Effects 0.000 description 1
- WDJUZGPOPHTGOT-XUDUSOBPSA-N digitoxin Chemical compound C1[C@H](O)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](C)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@@H]3C[C@@H]4[C@]([C@@H]5[C@H]([C@]6(CC[C@@H]([C@@]6(C)CC5)C=5COC(=O)C=5)O)CC4)(C)CC3)C[C@@H]2O)C)C[C@@H]1O WDJUZGPOPHTGOT-XUDUSOBPSA-N 0.000 description 1
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- BXFOFFBJRFZBQZ-PPFYZKLNSA-N insariotoxin Chemical compound C([C@]12[C@]3(C)[C@H](OC(C)=O)[C@@H](O)C1O[C@H]1[C@]3(COC(C)=O)C[C@@H](C(=C1)C)OC(=O)CC(C)C)O2 BXFOFFBJRFZBQZ-PPFYZKLNSA-N 0.000 description 1
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Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The utility model relates to a combined detection card capable of simultaneously detecting aflatoxin B1 and deoxynivalenol in various raw materials and finished products in feeds. The detection card comprises an upper plastic shell, a lower plastic shell and an internal colloidal gold test strip, wherein the upper plastic shell is provided with a sample feeding hole and an observing window; the lower plastic shell is provided with a protruded positioning trough; the colloidal gold test strip consists of a base plate, a reaction film, a sample pad, a gold mark combining pad and a water absorbing pad. The combined detection card has the characteristics of being strong in specificity, high in sensitivity, easy and fast to operate, low in cost, high in repeatability, wide in sample applicable range, simple and same in pretreatment process and the like; the aflatoxin B1 and the deoxynivalenol of the various raw materials and the finished products can be simultaneously detected, so that the time and the operation are greatly saved; the combined detection card is suitable for being used in field detection and large-scale detection and screening.
Description
Technical field
The utility model belongs to fast detecting field, is specifically related to a kind ofly can detect the aflatoxin B1 of the various raw materials of feed and finished product and the bigeminy test card of vomitoxin simultaneously.
Background technology
Mycotoxin (Mycotoxins) is the poisonous secondary metabolic product that mould produces in growth and breeding process on cereal or feed, toxin in the growth course of cereal, all can produce in the manufacture of feed, storage and transportation.According to FAO (Food and Agriculture Organization of the United Nation) (FAO) data, the crops in the whole world annual nearly 25% are subject to the pollution of mycotoxin to some extent, approximately have 2% crops because of the seriously polluted edibility that loses.There have been up to now 300 kinds of mycotoxins of surpassing to be isolated and identified out, mainly comprise aflatoxin (Aflatoxin, AFT), vomitoxin (Deoxynivalenol, DON), zearalenone (Zearalenone, ZEN), ochratoxin A (OchratoxinA, OTA), T-2 toxin (T-2toxin, T-2) etc.
Aflatoxin is one group of similar compound of chemical constitution, is mainly to be produced by aspergillus flavus and aspergillus parasiticus, comprises the kinds more than 10 such as B1, B2, G1 and G2, and wherein common with aflatoxin B1 (AFB1), toxicity is the strongest.AFB1 can destroy the digestive enzyme activity of pig, hinder its absorption to basic nutrition thing, reduce feed efficiency, cause pig body digestive function disorderly, anorexia, malnutrition, anaemia, immunosupress, weightening finish slow down, grow and stagnate and weaned piglets decline.Human body is taken in the food that polluted by aflatoxin B1 at short notice in a large number can cause acute poisoning, is mainly infringement liver, hepatitis occurs, cirrhosis, hepatonecrosis etc.Within 1993, aflatoxin is by (the Intemational Agency for Research onCancer of the cancer research mechanism of the World Health Organization (WHO), IARC) delimit as I class carcinogenic substance, wherein aflatoxin B1 is the strongest carcinogen that current occurring in nature exists.In China < < forage health standard > >, aflatoxin B1 residue limits in DIFFERENT FEED is stipulated, minimum limiting the quantity of is 10 μ g/kg.
Vomitoxin claims again deoxynivalenol, is the mould secondary metabolism product of sickle-like bacteria, mainly by Fusarium graminearum (Fusarium graminearum) and fusarium culmorum (Fusarium culmorum), is produced.Vomitoxin is prevalent in cereal and cereal products all over the world, and humans and animals is all had to very strong toxicity, can cause humans and animals vomiting, diarrhoea, skin irritatin, food refusal, neurological disorders, miscarriage, stillborn foetus etc.Pig is the animal the most responsive to vomitoxin, in feed, the micro-vomitoxin of 0.3～0.5mg/kg will cause that pig food refusal, growth decline and the resistibility of infectious disease is declined, in addition, vomitoxin also can, at humans and animals body accumulation, have teratogenesis, neurotoxicity, embryotoxicity and immunosuppressive action.Have data to show, vomitoxin is relevant with the mankind's alimentary toxic aleukia (ATA), and this mortality is higher, once in countries and regions such as USSR (Union of Soviet Socialist Republics), China and North Americas, occurs outburst.1998, in the appraisal report that international cancer research institution (IARC) announces, vomitoxin was listed in three class carcinogenic substances.The limiting the quantity of as 1mg/kg of deoxynivalenol in regulation swine feed in the allowance > > of deoxynivalenol in China < < mixed feed, calf mixed feed, lactation period animal mixed feed, in ox mixed feed and poultry mixed feed, content is no more than 5mg/kg.
The conventional sense method of aflatoxin B1, vomitoxin has very high requirement to instrument and equipment, environment, operative skill etc., and sense cycle is long, expensive, is seriously restricting the development of China's feed industry.Colloidal gold immunochromatographimethod technology has been a kind of method for quick that develop rapidly is got up since the eighties, because it has easy quick, simple to operate, high specificity, does not need the advantages such as extras, the fields such as clinical medicine, food security, environmental monitoring have been widely used at present.
In prior art, have separately the rapid detection card that aflatoxin B1 and vomitoxin are detected, but need to aflatoxin B1 and vomitoxin, detect respectively, operation repeats, time-consuming, wasting manpower and material resources.
Chinese patent application 201210473923.x discloses a kind of aflatoxin B1 in wheat and goods thereof that simultaneously detects, ochratoxin A, vomitoxin, the rapid detection card of zearalenone, but this test card is only applicable to the detection of wheat and goods thereof, the scope of application is not wide, practicality is not strong, can not meet completely Feedstuff Enterprises to various raw materials (as corn and goods thereof, wheat and goods thereof, crack rice, rice bran etc.) and the Quality Control of finished product, and the detectability for vomitoxin is 500 μ g/kg, can not meet Feedstuff Enterprises completely for the Quality Control requirement of vomitoxin.Therefore be badly in need of a kind ofly can detecting aflatoxin B1 and vomitoxin simultaneously, meeting and detect lower limit requirement, the testing tool that the scope of application is wide again.
Utility model content
The purpose of this utility model is to provide a kind of detection limit to meet the requirements, time saving and energy saving, the bigeminy test card of disposable detection aflatoxin B1, two kinds of toxin of vomitoxin.
For achieving the above object, the technical solution of the utility model is, a kind of aflatoxin B1, vomitoxin bigeminy test card, described bigeminy test card is by upper and lower two plastic housings and be placed in inner colloidal gold strip and form, and colloidal gold strip is comprised of sample pad (1), gold-marking binding pad (2), reaction film (3), base plate (4), adsorptive pads (5); Upper plastic housing is provided with well (10) and view window (11); Lower plastic housing be provided with protrusion in order to the fixing locating slot of colloidal gold strip;
Described gold-marking binding pad, reaction film, adsorptive pads join with horizontal direction order and are fixed on base plate top, the adjacent part of described gold-marking binding pad, reaction film, adsorptive pads respectively has the overlapping region of 1-2mm, on described gold-marking binding pad, be stained with sample pad, described base plate below is stained with adsorptive pads 2;
On described reaction film, from sample pad, to adsorptive pads direction, be provided with successively detection line 1, detection line 2 and nature controlling line, on detection line 1, be coated with the aflatoxin B1 artificial antigen that can be combined with aspergillus flavus resisting toxin B1 monoclonal antibody specificity, on detection line 2, be coated with can with the vomitoxin artificial antigen of emesis toxin monoclone antibody specific binding, on nature controlling line, be coated with the antiantibody that can be combined with aspergillus flavus resisting toxin B1 monoclonal antibody and emesis toxin monoclone antibody;
On described gold-marking binding pad, be coated with aspergillus flavus resisting toxin B1 monoclonal antibody and the emesis toxin monoclone antibody of colloid gold label.
Described base plate is Polyvinylchloride material, and described reaction film is nitrocellulose filter, and described sample pad and gold-marking binding pad are glass fibre membrane, and described adsorptive pads is filter paper.
Described detection line 1, detection line 2 are parallel to each other with nature controlling line and are vertical with sample flow direction, and detection line 1 and detection line 2 are at a distance of 3.5mm, and detection line 2 and nature controlling line are at a distance of 5mm.Detection line 1 and detection line 2 be at a distance of nearer, and with nature controlling line apart from each other, avoided pollution and the interference that may cause nature controlling line.Phase mutual interference between two kinds of toxin that distance between detection line 1 and detection line 2 has been avoided again detecting.
Described aflatoxin B1 artificial antigen is the conjugate of aflatoxin B1 and bovine serum albumin(BSA), and described vomitoxin artificial antigen is the conjugate of vomitoxin and bovine serum albumin(BSA), and described antiantibody is sheep anti-mouse igg.
Principle of the present utility model can be expressed as: the monoclonal anti physical efficiency of colloid gold label antiantibody on corresponding artificial antigen and nature controlling line on detection line is combined, and colloid gold particle is gathered on detection line and nature controlling line, presents macroscopic red stripes; If contain aflatoxin B1 or vomitoxin in sample solution, it can be combined with the monoclonal antibody of corresponding colloid gold label, thereby the monoclonal antibody that has hindered colloid gold label is attached on corresponding detection line, the red stripes on corresponding detection line is shoaled; If the aflatoxin B1 in solution or vomitoxin content have surpassed the detection limit of test card, corresponding detection line will not develop the color, and result is positive; Otherwise if the aflatoxin B1 in solution or vomitoxin content do not surpass the detection limit of test card, red stripes will appear in corresponding detection line, result is positive.
Therefore, compared with prior art, the utility model test card has following beneficial effect:
(1) many residue detection.The utility model test card has two detection lines, and aflatoxin B1 and the vomitoxin that can detect in feed residual simultaneously.
(2) highly sensitive.The aflatoxin B1 of the utility model test card and vomitoxin detection limit are respectively 5 μ g/kg and 200 μ g/kg, at feed, detect and have reached more excellent level, are applicable to all kinds of enterprises and testing agency.
(3) simple and quick.The pre-treatment of the utility model test card is simple, result is judged easily, only need a sample preparation and once drip plate can complete aflatoxin B1 in Feed Sample and the residual detection of vomitoxin, whole testing process can complete in 20 minutes, greatly shortened the sample time; And detect implementation process and do not rely on any experimental facilities, ordinary person all can operate, and does not need professional training.
(4) cost is low, easily promotes.The utility model test card production technology is simple, and flow process is ripe.Low production cost, requires less investment while yielding quicker results.
(5) practical.The utility model test card can be applicable to all feeds, as corn and goods, wheat and goods thereof, crack rice, the accuracy of rice bran and various finished products detects.
The utility model provides a kind of test card that can simultaneously detect aflatoxin B1 and vomitoxin, its detection limit is respectively 5 μ g/kg and 200 μ g/kg, only need a sample preparation and once drip plate can complete aflatoxin B1 in Feed Sample and the residual detection of vomitoxin, simple and convenient, time saving and energy saving, greatly shorten the sample time, be suitable for on-site supervision and basic unit's Large-scale Screening detection.
Accompanying drawing explanation
Fig. 1 is the side structure schematic diagram of colloidal gold strip in the utility model test card.Wherein 1 is sample pad, and 2 is gold-marking binding pad, and 3 is reaction film, and 4 is base plate, and 5 is adsorptive pads, and 7 is that detection line 1,8 is that detection line 2,9 is nature controlling line.
Fig. 2 is the utility model test card vertical view, and wherein 10 is well, and 11 is view window.
Embodiment
Below in conjunction with specific embodiment, further set forth the utility model.Should be understood that these embodiment are only not used in restriction scope of the present utility model for the utility model is described.In addition should be understood that, after having read content of the present utility model, those skilled in the art can make various changes or modifications the utility model, these equivalent form of values fall within the utility model limited range equally.
A kind of aflatoxin B1 as depicted in figs. 1 and 2, vomitoxin bigeminy test card, test card comprises upper and lower two plastic housings and is placed in inner colloidal gold strip, the plastic housing of top is provided with well (10) and view window (11), and the plastic housing of below has the locating slot of protrusion; Colloidal gold strip comprises base plate (4), reaction film (3), sample pad (1), gold-marking binding pad (2), adsorptive pads (5), and reaction film (3) is provided with detection line 1 (7), detection line 2 (8) and nature controlling line (9); Well (10) on the plastic housing of the corresponding top of sample pad (1), view window (11) on the plastic housing of the corresponding top of reaction film (3); For guaranteeing mobile the carrying out smoothly of sample solution, gold-marking binding pad (2) laminates in nearly sample pad (1) end of reaction film (3), sample pad (1) laminates in gold-marking binding pad (2) upper, and adsorptive pads (5) laminates in reaction film (3) sample pad (1) end far away.
From packaging bag, take out test card, draw sample solution 100 μ L to be checked and be added drop-wise in well, after application of sample, start timing; Result should read at 5～8min, and other times interpretation is invalid.During observation, test card is placed horizontally at observer front.
Result interpretation:
Negative (-): detection line T1 line has colour developing, shows in sample that aflatoxin B1 residual content is lower than 5ppb or not residual containing aflatoxin B1; Detection line T2 line has colour developing, shows in sample that vomitoxin residual content is lower than 200ppb or not residual containing vomitoxin;
Positive (+): detection line T1 line, without colour developing, shows in sample that aflatoxin B1 residual content is higher than 5ppb; Detection line T2 line, without colour developing, shows in sample that vomitoxin residual content is higher than 200ppb;
Invalid: not occurring C line, may be that misoperation or test card lost efficacy.Should again read instructions, and retest by new test card.
The utility model can detect aflatoxin B1 and two kinds of toxin of vomitoxin simultaneously, its detection limit is respectively 5 μ g/kg and 200 μ g/kg, only need a sample preparation and once drip plate can complete aflatoxin B1 in Feed Sample and the residual detection of vomitoxin, simple and convenient, time saving and energy saving, greatly shorten the sample time, be suitable for on-site supervision and basic unit's Large-scale Screening detection.
Be more than the description to the utility model embodiment, by the above-mentioned explanation to the disclosed embodiments, make professional and technical personnel in the field can realize or use the utility model.To the multiple modification of these embodiment, will be apparent for those skilled in the art, General Principle as defined herein can, in the situation that not departing from spirit or scope of the present utility model, realize in other embodiments.Therefore, the utility model will can not be restricted to these embodiment shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (4)
1. an aflatoxin B1, vomitoxin bigeminy test card, it is characterized in that, described bigeminy test card is by upper and lower two plastic housings and be placed in inner colloidal gold strip and form, and colloidal gold strip is comprised of sample pad (1), gold-marking binding pad (2), reaction film (3), base plate (4), adsorptive pads (5); Upper plastic housing is provided with well (10) and view window (11); Lower plastic housing be provided with protrusion in order to the fixing locating slot of colloidal gold strip;
Described gold-marking binding pad, reaction film, adsorptive pads join with horizontal direction order and are fixed on base plate top, and the adjacent part of described gold-marking binding pad, reaction film, adsorptive pads respectively has the overlapping region of 1-2mm, on described gold-marking binding pad, is stained with sample pad;
On described reaction film, from sample pad to adsorptive pads direction, be provided with successively detection line 1 (7), detection line 2 (8) and nature controlling line (9), on detection line 1, be coated with the aflatoxin B1 artificial antigen that can be combined with aspergillus flavus resisting toxin B1 monoclonal antibody specificity, on detection line 2, be coated with can with the vomitoxin artificial antigen of emesis toxin monoclone antibody specific binding, on nature controlling line, be coated with the antiantibody that can be combined with aspergillus flavus resisting toxin B1 monoclonal antibody and emesis toxin monoclone antibody;
On described gold-marking binding pad, be coated with aspergillus flavus resisting toxin B1 monoclonal antibody and the emesis toxin monoclone antibody of colloid gold label.
2. aflatoxin B1 as claimed in claim 1, vomitoxin bigeminy test card, it is characterized in that, described detection line 1, detection line 2 are parallel to each other with nature controlling line and are vertical with sample flow direction, and detection line 1 and detection line 2 are at a distance of 3.5mm, and detection line 2 and nature controlling line are at a distance of 5mm.
3. aflatoxin B1 as claimed in claim 1, vomitoxin bigeminy test card, it is characterized in that, described base plate is Polyvinylchloride material, and described reaction film is nitrocellulose filter, described sample pad and gold-marking binding pad are glass fibre membrane, and described adsorptive pads is filter paper.
4. aflatoxin B1 as claimed in claim 1, vomitoxin bigeminy test card, it is characterized in that, described aflatoxin B1 artificial antigen is the conjugate of aflatoxin B1 and bovine serum albumin(BSA), described vomitoxin artificial antigen is the conjugate of vomitoxin and bovine serum albumin(BSA), and described antiantibody is sheep anti-mouse igg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420082102.8U CN203838157U (en) | 2014-02-26 | 2014-02-26 | Combined detection card for aflatoxin B1 and deoxynivalenol |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420082102.8U CN203838157U (en) | 2014-02-26 | 2014-02-26 | Combined detection card for aflatoxin B1 and deoxynivalenol |
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| Publication Number | Publication Date |
|---|---|
| CN203838157U true CN203838157U (en) | 2014-09-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201420082102.8U Expired - Lifetime CN203838157U (en) | 2014-02-26 | 2014-02-26 | Combined detection card for aflatoxin B1 and deoxynivalenol |
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| CN (1) | CN203838157U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106940374A (en) * | 2017-05-09 | 2017-07-11 | 河南知微生物工程有限公司 | A kind of test card of quick detection vomitoxin |
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2014
- 2014-02-26 CN CN201420082102.8U patent/CN203838157U/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106940374A (en) * | 2017-05-09 | 2017-07-11 | 河南知微生物工程有限公司 | A kind of test card of quick detection vomitoxin |
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Address after: 215417 Jiangsu city of Suzhou province Taicang city Shaxi town Xingang Yuewang Road No. 239 Patentee after: ANYOU BIOTECHNOLOGY GROUP Co.,Ltd. Address before: 215417 Jiangsu city of Suzhou province Taicang city Shaxi town Xingang Yuewang Road No. 239 Patentee before: ANYOU BIOTECHNOLOGY GROUP CO.,LTD. |
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Granted publication date: 20140917 |