CN203833921U - Pipette tip inserting and removing device for purification and separation of biomolecules - Google Patents
Pipette tip inserting and removing device for purification and separation of biomolecules Download PDFInfo
- Publication number
- CN203833921U CN203833921U CN201420084672.0U CN201420084672U CN203833921U CN 203833921 U CN203833921 U CN 203833921U CN 201420084672 U CN201420084672 U CN 201420084672U CN 203833921 U CN203833921 U CN 203833921U
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- open tube
- installation frame
- liquid transfer
- inserting device
- gun head
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Abstract
The utility model relates to the technical field of purification and separation of biomolecules and particularly relates to a pipette tip inserting and removing device for purification and separation of biomolecules. The pipette tip inserting and removing device comprises a mounting frame, a hollow tube, a stopper and an elastic buffer part, wherein the hollow tube penetrates through a lower rim of the mounting frame, the hollow tube is located in the frame by virtue of the stopper arranged on the hollow tube, the elastic buffer part is arranged between an upper rim and the stopper, in the process of inserting and removing the tip to be installed, under the action of a slider mechanism, the mounting frame moves downwards, when the lower end of the hollow tube is in contact with the tip, a certain relative movement is produced between the hollow tube and the mounting frame, a pressing force generated by compressing the elastic buffer part enables the lower end of the hollow tube to be in buffer butt-joint with the tip, the operation that the tip is stably and reliably inserted into or removed from the lower end of the hollow tube can be achieved, and then the deflection of inserted and removed tip can be prevented, and a good seal between the lower end of the hollow tube and tip is achieved.
Description
Technical field
The utility model relates to the purifies and separates technical field of biomolecules, relates in particular to a kind of liquid transfer gun head inserting device separating for biomolecules purification.
Background technology
At present, mainly comprise for the Nucleotide extracting and purifying method of biomolecules: (1) cracking: preparation one is loaded with the centrifuge tube of lysate, will treat that manipulating cells is placed in centrifuge tube and carries out cracking, makes cell rupture with released dna; (2) combination: employing magnetic bead etc., as solid phase carrier, are placed in centrifuge tube by solid phase carrier, and the DNA discharging is combined with solid phase carrier; (3) clean:
The magnet stand (as magnetism stick etc.) that is set with sheath is insinuated in centrifuge tube and adsorbs magnetic bead, and then hand-held liquid-transfering device is extracted out in conjunction with liquid, then magnetic bead is cleaned, and then extracts scavenging solution out; (4) wash-out: inject elutriant in centrifuge tube, then remove the magnet stand in sheath, magnetic bead departs from sheath and falls into the elutriant in centrifuge tube, makes DNA depart from magnetic bead by modes such as heating and sneaks into the DNA that obtains purifying in elutriant.
At present, the Nucleotide purification process of biomolecules has been realized automated job, the Chinese patent application that application number is 2013104710490 provides a kind of automatic purifies and separates system, in the Nucleotide purification process of biomolecules, need various liquid to shift, filling apparatus in above-mentioned automatic purifies and separates system comprises multiple-head type filling gun, reagent trough and slide block mechanism, multiple-head type filling gun is arranged on slide block mechanism and can moves along vertical and horizontal direction, multiple-head type filling gun is connected with a suction means (being generally suction pumps) by a flexible pipe, thereby can pipette easily liquid, due to technologic requirement, rifle head is disposable rifle head, need often to change, in the process of automatic job, it is also to complete by automatization that rifle changes first watch, be generally multiple-head type filling gun is moved to the support top of placing rifle head to be installed, to take off with rifle head, exchange rifle head to be installed for, but change in the process of installation first watch at rifle, due to the existence of rifle head to be installed and multiple-head type rifle head filling gun error in mounting position, often there is problem crooked by rifle head and that sealing is bad.
Therefore,, for above deficiency, the utility model provides a kind of liquid transfer gun head inserting device separating for biomolecules purification.
Utility model content
(1) technical problem that will solve
The purpose of this utility model is to provide a kind ofly changes at rifle the problem that rifle head is crooked and sealing is bad that installation process exists to solve existing liquid-transfering device first watch for the biomolecules liquid transfer gun head inserting device separating of purifying.
(2) technical scheme
In order to solve the problems of the technologies described above, the utility model provides a kind of liquid transfer gun head inserting device separating for biomolecules purification, it comprises installation frame, open tube, block piece, resilient snubber, sealing member and connecting hose, installation frame is arranged on slide block mechanism, open tube is through the lower frame of installation frame, and there is gap between open tube and installation frame, block piece is arranged on open tube, and block piece is positioned at the top of lower frame, resilient snubber is arranged between edge and block piece, sealing member is arranged on the lower end of open tube, one end of connecting hose is connected with imbibition parts, the other end of connecting hose is connected with the upper end of open tube, or be connected with the lower end of open tube through open tube.
Wherein, the upper end of described open tube is through the upper edge of installation frame, and resilient snubber is set on open tube.
Wherein, the upper end of described open tube is connected by a T-shaped joint nut with connecting hose, T-shaped joint nut's lower end is stretched into the upper end of open tube and is coordinated with the inside thread of open tube, one end that connecting hose is connected with open tube is through backward outer the digging T-shaped joint nut's outside, lower end and the inner side of open tube of T-shaped joint nut, and resilient snubber is set in T-shaped joint nut and connecting hose.
Wherein, the side of described installation frame arranges a mounting plate, and installation frame is arranged on slide block mechanism by this mounting plate.
Wherein, described resilient snubber is spring.
Wherein, the lower end of described open tube is arranged with O-ring seal.
Wherein, described open tube is integrally formed with block piece or split is connected.
Wherein, the material of described open tube is metal or plastics.
Wherein, in described installation frame, multiple open tubes are set.
Wherein, in described installation frame, be set up in parallel four, eight or 12 open tubes.
(3) beneficial effect
Technique scheme tool of the present utility model has the following advantages: in the liquid transfer gun head inserting device that the utility model provides, open tube is through the lower frame of installation frame, open tube is arranged in framework by block piece disposed thereon, resilient snubber is arranged between edge and block piece, insert and get in the process of rifle head to be installed, under the effect of slide block mechanism, installation frame moves down, in the time that open tube lower end touches rifle head, open tube and installation frame produce certain relative movement, the snap-in force of the compressed rear generation of resilient snubber makes the lower end of open tube dock with rifle head buffering, can realize the reliable head of taking arms of inserting reliably in lower end of open tube, and then can prevent that the rifle head after slotting getting is crooked, and the lower end of open tube and the excellent sealing of rifle head are realized.
Brief description of the drawings
Fig. 1 is the stereographic map of the utility model embodiment for the liquid transfer gun head inserting device of biomolecules purification separation;
Fig. 2 is that the liquid transfer gun head inserting device that the utility model embodiment separates for biomolecules purification arranges the rear view after mounting plate.
In figure, 1: installation frame; 2: open tube; 3: block piece; 4: spring; 5: O-ring seal; 6: mounting plate.
Embodiment
Below in conjunction with drawings and Examples, embodiment of the present utility model is described in further detail.Following examples are used for illustrating the utility model, but are not used for limiting scope of the present utility model.
As shown in Figure 1, the liquid transfer gun head inserting device separating for biomolecules purification that the utility model provides, it comprises installation frame 1, eight open tubes 2, block piece 3, spring 4 and O-ring seal 5, installation frame 1 is arranged on slide block mechanism, under the effect of slide block mechanism installation frame 1 can level and vertical direction move, on the underframe of installation frame 1, be provided with eight through holes that are arranged side by side, on the upper frame of installation frame 1, be provided with the corresponding groove in hole with underframe, open tube 2 is through the groove of upper edge and the through hole of lower frame of installation frame 1, between the through hole of open tube and installation frame and groove, there is gap, on open tube 2, be provided with block piece 3, block piece 3 is positioned at the top of lower frame, can make open tube 2 be arranged in framework by this block piece 3, spring 4 is set on open tube 2, spring 4 is between upper edge and block piece 3, when inserting while taking arms, one end of spring 4 is against below edge, the other end is against on block piece 3, O-ring seal 5 is set in the lower end of open tube 2, the coupling end of rifle head is inserted in the lower end of open tube 2, can make open tube 2 and rifle head be tightly connected by O-ring seal 5, the upper end of open tube 2 is connected with imbibition parts (being generally suction pumps) by a flexible pipe, air-breathing by these imbibition parts, can make the interior generation negative pressure of open tube 2, liquid in containers is sucked in rifle head, or by this imbibition parts venting, the middle liquid of rifle head is emitted.
In technique scheme, because rifle head needs to change in time, in the time changing rifle head, move by slide block mechanism, liquid transfer gun head inserting device of the present utility model is transferred to the support top of placing rifle head, open tube 2 moves down, in the time that the lower end of open tube 2 O-ring seal 5 touches rifle head, open tube 2 no longer moves down, and installation frame 1 continues to move down, open tube 2 produces certain relative movement with installation frame 1, the restriction lower compression of the edge on the low gear of block piece 3 and installation frame 1 of the spring 4 in installation frame 1, under the thrust of installation frame 1, the snap-in force of spring 4 makes the lower end of open tube 2 dock with rifle head buffering, realize the reliable head of taking arms of inserting reliably in lower end of open tube 2, can prevent that like this rifle head after slotting getting is crooked, and can make the lower end of open tube 2 and rifle head realize good sealing, guarantee moves completing smoothly of liquid.
Wherein, the lower end of open tube also can not arrange O-ring seal, and the tube wall of open tube lower end can be thinner, so that insert while taking arms and rifle head is tightly connected.
Certainly, in the utility model, the lower frame of installation frame 1 can only be passed in the upper end of open tube 2, block piece is arranged on to the upper end of open tube 2, the upper end of open tube 2 is connected by a T-shaped joint nut with flexible pipe, T-shaped joint nut's lower end is stretched into the upper end of open tube 2 and is coordinated with the inside thread of open tube 2, one end that connecting tube is connected with open tube 2 is through backward outer the digging T-shaped joint nut's outside, lower end and the inner side of open tube of T-shaped joint nut, and resilient snubber is set on T-shaped joint nut and connecting tube.The materials that can save so on the one hand open tube, flexible pipe and open tube 2 are stronger by stopping property and the reliability of T-shaped joint nut's this mode of connection on the other hand.
Certainly,, in technique scheme, one end of flexible pipe also can be connected with the lower end of open tube from the upper end of open tube through open tube.Particularly, this mode of connection can, for being turned up in this one end of flexible pipe in the outside, lower end of open tube, then cling with glue, or be other conventional mode of connection.
Certainly, described spring 4 also can be replaced by other resilient snubbers; The number of described installation frame 1 hollow core barrel 2 can be also four, eight, 12 or other numbers, multiple open tubes 2 are set and can realize batch production, the arrangement mode of open tube 2 can be arranged side by side or array is arranged, and can be convenient to like this processing and be beneficial to operation; Wherein, the block piece 3 being arranged on open tube 2 is ring-type or square, and this block piece 3 can be integrally formed with open tube 2, also can split connect, and the material of open tube 2 can be metal or plastics.
Further, as shown in Figure 2, the side of described installation frame 1 arranges a mounting plate 6, and this mounting plate 6 is arranged on slide block mechanism, can facilitate being connected and dismounting of installation frame 1 and slide block mechanism by mounting plate 6.
The liquid transfer gun head inserting device that the utility model provides in use, installation frame 1 is arranged on slide block mechanism by mounting plate 6, under the effect of slide block mechanism, liquid transfer gun head inserting device of the present utility model is moved to the support top of rifle head to be installed, remove and used rifle head, installation frame 1 moves down, open tube 2 also moves down until touch rifle head thereupon, then the restriction lower compression of spring 4 edge on the low gear of block piece 3 and installation frame 1, in the process of compressing at spring 4, the lower end of open tube 2 is reliable inserts and gets rifle head to be installed reliably.
In sum, in the liquid transfer gun head inserting device that the utility model provides, by open tube 2 is arranged in installation frame 1, block piece 3 is set on open tube 2, spring 4 is set on open tube 2, and between upper edge and block piece 3, insert and get in rifle head process to be installed, installation frame 1 moves down, open tube 2 produces certain relative movement with installation frame 1, the snap-in force producing after spring 4 compressions makes the lower end of open tube 2 dock with rifle head buffering, realize the reliable head of taking arms of inserting reliably in lower end of open tube 2, prevent from inserting and got the crooked of rear rifle head, and the lower end of open tube 2 and the excellent sealing of rifle head are realized, can insert simultaneously and get multiple rifle heads by multiple open tubes 2 are set, realize batch production, by being set, mounting plate 6 can facilitate the installation and removal of installation frame 1.
The above is only preferred implementation of the present utility model; should be understood that; for those skilled in the art; do not departing under the prerequisite of the utility model know-why; can also make some improvement and modification, these improve and modification also should be considered as protection domain of the present utility model.
Claims (10)
1. the liquid transfer gun head inserting device separating for biomolecules purification, it is characterized in that: it comprises installation frame, open tube, block piece, resilient snubber and connecting hose, installation frame is arranged on slide block mechanism, open tube is through the lower frame of installation frame, and there is gap between open tube and installation frame, block piece is arranged on open tube, and block piece is positioned at the top of lower frame, resilient snubber is arranged between edge and block piece, one end of connecting hose is connected with imbibition parts, the other end of connecting hose is connected with the upper end of open tube, or be connected with the lower end of open tube through open tube.
2. liquid transfer gun head inserting device according to claim 1, is characterized in that: the upper end of described open tube is through the upper edge of installation frame, and resilient snubber is set on open tube.
3. liquid transfer gun head inserting device according to claim 1, it is characterized in that: the upper end of described open tube is connected by a T-shaped joint nut with connecting hose, T-shaped joint nut's lower end is stretched into the upper end of open tube and is coordinated with the inside thread of open tube, one end that connecting hose is connected with open tube is through backward outer the digging T-shaped joint nut's outside, lower end and the inner side of open tube of T-shaped joint nut, and resilient snubber is set in T-shaped joint nut and connecting hose.
4. liquid transfer gun head inserting device according to claim 1, is characterized in that: the side of described installation frame arranges a mounting plate, installation frame is arranged on slide block mechanism by this mounting plate.
5. liquid transfer gun head inserting device according to claim 1, is characterized in that: described resilient snubber is spring.
6. liquid transfer gun head inserting device according to claim 1, is characterized in that: the lower end of described open tube is arranged with O-ring seal.
7. liquid transfer gun head inserting device according to claim 1, is characterized in that: described open tube is integrally formed with block piece or split is connected.
8. liquid transfer gun head inserting device according to claim 7, is characterized in that: the material of described open tube is metal or plastics.
9. according to the liquid transfer gun head inserting device described in claim 1-8 any one, it is characterized in that: multiple open tubes are set in described installation frame.
10. liquid transfer gun head inserting device according to claim 9, is characterized in that: in described installation frame, be set up in parallel four, eight or 12 open tubes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420084672.0U CN203833921U (en) | 2014-02-26 | 2014-02-26 | Pipette tip inserting and removing device for purification and separation of biomolecules |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420084672.0U CN203833921U (en) | 2014-02-26 | 2014-02-26 | Pipette tip inserting and removing device for purification and separation of biomolecules |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203833921U true CN203833921U (en) | 2014-09-17 |
Family
ID=51511789
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420084672.0U Expired - Fee Related CN203833921U (en) | 2014-02-26 | 2014-02-26 | Pipette tip inserting and removing device for purification and separation of biomolecules |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203833921U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103897980A (en) * | 2014-02-26 | 2014-07-02 | 北京中纳海润生物科技有限公司 | Liquid moving gun inserting device for biologic molecule purification and separation |
| CN110215944A (en) * | 2019-07-08 | 2019-09-10 | 昆山汇先医药技术有限公司 | A kind of automatic liquid-transfering gun |
-
2014
- 2014-02-26 CN CN201420084672.0U patent/CN203833921U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103897980A (en) * | 2014-02-26 | 2014-07-02 | 北京中纳海润生物科技有限公司 | Liquid moving gun inserting device for biologic molecule purification and separation |
| CN103897980B (en) * | 2014-02-26 | 2016-02-10 | 聂棱 | A kind of liquid transfer gun head inserting device for biomolecules purification & isolation |
| CN110215944A (en) * | 2019-07-08 | 2019-09-10 | 昆山汇先医药技术有限公司 | A kind of automatic liquid-transfering gun |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140917 Termination date: 20210226 |