CN202576433U - Cell dissociation blowing and injecting gun for dissociation and passage of culture cells - Google Patents
Cell dissociation blowing and injecting gun for dissociation and passage of culture cells Download PDFInfo
- Publication number
- CN202576433U CN202576433U CN 201220217180 CN201220217180U CN202576433U CN 202576433 U CN202576433 U CN 202576433U CN 201220217180 CN201220217180 CN 201220217180 CN 201220217180 U CN201220217180 U CN 201220217180U CN 202576433 U CN202576433 U CN 202576433U
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- rifle
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Abstract
The utility model discloses a cell dissociation blowing and injecting gun for dissociation and passage of culture cells. The cell dissociation blowing and injecting gun comprises a suction head portion and a gun portion which are connected. The suction head portion is a suction head with scale marks, and an overflow-preventive filter layer is arranged inside the suction head. The gun portion comprises a gun head, a gun body and a handle in mutual connection. The cell dissociation blowing and injecting gun can effectively prevent pollution caused overflow and refluxing after suction of solution when being used for dissociation and passage of the culture cells, is flexible and convenient to operate during cell blowing and injecting and solution transferring, and can be used for multiple purposes such as solution transferring and filtering. When in use of the cell dissociation blowing and injecting gun, a user can sleeve the suction head portion into the gun portion, press a pressure rod on the gun portion to enable a spring to retract to drive a pushing rod to move forwards in linkage to push a spring on a core rod so that the core rod can move forwards to generate positive pressure (blowing out solution), and release the pressure rod so that the spring is reset to drive the pushing rod in linkage to drive the core rod to retract to generate negative pressure (sucking the solution).
Description
Technical field
The utility model relates to the attached cell had digestive transfer culture equipment of cultivating in molecular biosciences and the Cell Biology Experiment, and especially a kind of cell dissociation piping and druming rifle that is used for the use of culturing cell had digestive transfer culture is applicable to and moves liquid, filtration multiple use.
Background technology
So far, also there is not a kind of cell dissociation piping and druming equipment that uses to the culturing cell had digestive transfer culture both at home and abroad.For a long time, during the culturing cell had digestive transfer culture, adopt the glass pipette end to put the rubber rubber pipette bulb and come the adherent growth cell in the imbitition piping and druming culturing bottle, or adopt electronic pipettor to blow and beat peptic cell in molecular biosciences and the Cell Biology Experiment.These two kinds of equipment are made troubles usually for experimental implementation person and are caused cell contamination.Because two kinds of equipment all use longer glass pipette, the place of corner in the culturing bottle is blown and beaten less than forming the dead angle.And easily liquid is drawn in the ball (or in pipettor pump) and causes that solution is counter to flow pollution, and glass pipette be prone to broken, pipettor needs often to change battery, move liquid pump is prone to stop up the shortcoming and defect of damaging and cost an arm and a leg etc.
Summary of the invention
In order to improve the shortcoming and defect of above two kinds of equipment; The purpose of the utility model is; A kind of cell dissociation piping and druming rifle (device) that the culturing cell had digestive transfer culture is used that is used for is provided; Not only flexible operation is easy for this cell dissociation piping and druming rifle (device), and replaceable aseptic suction nozzle, can not cause cell contamination when doing the cell had digestive transfer culture.
In order to realize above-mentioned task, the utility model solves the technical scheme that its technical problem adopted and is:
A kind of cell dissociation piping and druming rifle that is used for the use of culturing cell had digestive transfer culture; It is characterized in that; Connect to form by suction nozzle part and rifle body portion; Described suction nozzle partly for having graduated suction nozzle, be equipped with anti-liquid and overflow filtering layer by suction nozzle inside, and described rifle body portion comprises interconnective rifle head, gun body part and handle.
Some other characteristics of the utility model are:
The core bar and first spring are equipped with in described rifle head inside; On the described gun body part window is arranged, on window, be provided with window Ka Gai, interlock push rod and the turning joint that has depression bar are housed in gun body part inside, turning joint is fixed through the depression bar point of fixity; Between depression bar and handle, also be provided with second spring.
Adopt between described rifle head, gun body part and the handle and be threaded.
Described suction nozzle adopts the disposable plastic suction nozzle.
The cell dissociation piping and druming rifle that is used for the use of culturing cell had digestive transfer culture of the utility model; In the time of can making the culturing cell had digestive transfer culture; The pollution of overflowing anti-stream after effectively stoping solution to suck and causing, flexible and convenient operation when blowing and beating cell and moving liquid, and can be used for moving liquid, filtration multiple use.During use suction nozzle partly is inserted in the rifle body portion, the depression bar of pressing on the rifle body portion shrinks spring, and the reach of interlock push rod, the spring that promotes on the core bar make the core bar reach produce malleation (blowing out liquid).Unclamp depression bar and make the spring return, the interlock push rod drives the core bar retraction and produces negative pressure (suction liquid).
Description of drawings
Fig. 1 is cell dissociation piping and druming rifle (device) outside drawing that the culturing cell had digestive transfer culture is used that is used for of the utility model;
Fig. 2 is the synoptic diagram that Fig. 1 is decomposed into suction nozzle part and rifle body portion;
Fig. 3 is the synoptic diagram that Fig. 2 is further decomposed;
Fig. 4 is the sectional view of Fig. 1.
Fig. 5 is decomposition, the sectional structure chart of Fig. 3.
Mark among the figure is represented respectively: 1, suction nozzle, 2, filtering layer, 3, rifle head (core tube), 4, first interface, 5, first spring; 6, core bar, 7, first screw thread, 8, gun body part (core tube), 9, window Ka Gai, 10, turning joint; 11, second interface, 12, the interlock push rod, 13, depression bar, 14, the depression bar point of fixity; 15, window, 16, second screw thread, 17, handle, 18, second spring.
Below in conjunction with accompanying drawing and embodiment utility model is done further explain.
Embodiment
Referring to accompanying drawing 1 ~ 5, present embodiment provides a kind of cell dissociation piping and druming rifle that the culturing cell had digestive transfer culture is used that is used for, and is connected to form by suction nozzle part and rifle body portion.
Suction nozzle partly for cone-shaped have a graduated suction nozzle 1, suction nozzle 1 inside is equipped with anti-liquid and is overflowed filtering layer 2, the rifle body portion comprises interconnective rifle head 3, gun body part 8 and handle 17.
The core bar 6 and first spring 5 are equipped with in rifle head 3 inside; Window 15 is arranged on the gun body part 8, on window 15, be provided with window card lid 9, interlock push rod 12 and the turning joint 10 that has depression bar 13 are housed in gun body part 8 inside, turning joint 10 is fixing through depression bar point of fixity 14, and can rotate around depression bar point of fixity 14; Between depression bar 13 and handle 17, also be provided with second spring 18.
In the present embodiment, adopt between rifle head 3, gun body part 8 and the handle 17 to be threaded.End like the rifle head 3 among Fig. 5 has first interface, 4, the first interfaces 4 to be connected with first screw thread 7 of gun body part 8, and second interface 11 of gun body part 8 is connected with second screw thread 16 on the handle 17.
In the present embodiment, suction nozzle 1 adopts the disposable plastic suction nozzle.Can suction nozzle 1 be carried out autoclaving according to the experiment needs.
During use; Suction nozzle partly is inserted in the rifle body portion, presses the depression bar 13 on the rifle body portion, first spring 5 and second spring 18 are shunk simultaneously; Under the effect of turning joint 10,12 reaches of interlock push rod, first spring 5 that promotes on the core bar 6 make core bar 6 reaches produce malleation (blowing out liquid).Unclamp depression bar 13 and make first spring 5 and second spring, 18 constraint reliefs, interlock push rod 12 returns, under the retraction of first spring 5, core bar 6 returns produce negative pressure (suction liquid).
The cell dissociation piping and druming rifle that is used for the use of culturing cell had digestive transfer culture of the utility model; Can draw in the bottle liquid blows and beats attached cell repeatedly; Cell is come off from the bottle wall be dispersed into uniform individual cells suspension; Again cell suspension is passed 2 or pass 4 and be drawn in the clean culturing bottle by equivalent 1, thereby reach the cell dissociation purpose that goes down to posterity.
Claims (4)
1. one kind is used for the cell dissociation piping and druming rifle that the culturing cell had digestive transfer culture is used; It is characterized in that; Connect to form by suction nozzle part and rifle body portion; Described suction nozzle partly for having graduated suction nozzle (1), be equipped with anti-liquid and overflow filtering layer (2) by suction nozzle (1) inside, and described rifle body portion comprises interconnective rifle head (3), gun body part (8) and handle (17).
2. the cell dissociation piping and druming rifle that is used for the use of culturing cell had digestive transfer culture as claimed in claim 1 is characterized in that core bar (6) and first spring (5) are equipped with in described rifle head (3) inside; Window (15) is arranged on the described gun body part (8); On window (15), be provided with window Ka Gai (9); In gun body part (8) inside interlock push rod (12) is housed and has the turning joint (10) of depression bar (13), turning joint (10) is fixing through depression bar point of fixity (14); Between depression bar (13) and handle (17), also be provided with second spring (18).
3. the cell dissociation piping and druming rifle that is used for the use of culturing cell had digestive transfer culture as claimed in claim 2 is characterized in that, adopts between described rifle head (3), gun body part (8) and the handle (17) to be threaded.
4. the cell dissociation piping and druming rifle that is used for the use of culturing cell had digestive transfer culture as claimed in claim 1 is characterized in that described suction nozzle (1) adopts the disposable plastic suction nozzle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220217180 CN202576433U (en) | 2012-05-15 | 2012-05-15 | Cell dissociation blowing and injecting gun for dissociation and passage of culture cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220217180 CN202576433U (en) | 2012-05-15 | 2012-05-15 | Cell dissociation blowing and injecting gun for dissociation and passage of culture cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202576433U true CN202576433U (en) | 2012-12-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220217180 Expired - Fee Related CN202576433U (en) | 2012-05-15 | 2012-05-15 | Cell dissociation blowing and injecting gun for dissociation and passage of culture cells |
Country Status (1)
| Country | Link |
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| CN (1) | CN202576433U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105176812A (en) * | 2015-07-28 | 2015-12-23 | 浙江奥瑞健生物技术有限公司 | Stem cell globe cutting and collecting device and stem cell globe cutting and separating passage method |
| CN107603870A (en) * | 2017-11-10 | 2018-01-19 | 上海保兴生物设备工程有限公司 | A kind of digestion filter for micro-carriers cell culture |
| CN108410714A (en) * | 2018-03-16 | 2018-08-17 | 苏杰 | A kind of cell mixing liquid-transfering gun |
| CN111925923A (en) * | 2020-09-17 | 2020-11-13 | 天津速一腾高新技术有限公司 | A extrusion is blown and is beaten device for breaking up cell |
-
2012
- 2012-05-15 CN CN 201220217180 patent/CN202576433U/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105176812A (en) * | 2015-07-28 | 2015-12-23 | 浙江奥瑞健生物技术有限公司 | Stem cell globe cutting and collecting device and stem cell globe cutting and separating passage method |
| CN105176812B (en) * | 2015-07-28 | 2017-10-03 | 浙江奥瑞健生物技术有限公司 | A kind of cutting separation propagating method of stem cell sphere cutting collection device and stem cell sphere |
| CN107603870A (en) * | 2017-11-10 | 2018-01-19 | 上海保兴生物设备工程有限公司 | A kind of digestion filter for micro-carriers cell culture |
| CN107603870B (en) * | 2017-11-10 | 2023-06-02 | 上海保兴生物设备工程有限公司 | Digestion and filtration device for microcarrier cell culture |
| CN108410714A (en) * | 2018-03-16 | 2018-08-17 | 苏杰 | A kind of cell mixing liquid-transfering gun |
| CN108410714B (en) * | 2018-03-16 | 2021-05-04 | 苏州亚通生物医疗科技有限公司 | Cell mixing pipette |
| CN111925923A (en) * | 2020-09-17 | 2020-11-13 | 天津速一腾高新技术有限公司 | A extrusion is blown and is beaten device for breaking up cell |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121205 Termination date: 20130515 |