CN203798642U - Closed type crop leaf surface secreta cycle extraction device - Google Patents
Closed type crop leaf surface secreta cycle extraction device Download PDFInfo
- Publication number
- CN203798642U CN203798642U CN201420105616.0U CN201420105616U CN203798642U CN 203798642 U CN203798642 U CN 203798642U CN 201420105616 U CN201420105616 U CN 201420105616U CN 203798642 U CN203798642 U CN 203798642U
- Authority
- CN
- China
- Prior art keywords
- crop leaf
- secreta
- separating funnel
- leaf surface
- extraction device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000605 extraction Methods 0.000 title abstract description 18
- 239000011521 glass Substances 0.000 claims abstract description 21
- 230000028327 secretion Effects 0.000 claims description 20
- 238000000967 suction filtration Methods 0.000 claims description 15
- 239000003960 organic solvent Substances 0.000 abstract description 4
- 230000007547 defect Effects 0.000 abstract description 2
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 63
- 239000000706 filtrate Substances 0.000 description 7
- 238000004821 distillation Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 3
- 238000009833 condensation Methods 0.000 description 3
- 230000005494 condensation Effects 0.000 description 3
- 238000001704 evaporation Methods 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000013459 approach Methods 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 210000001508 eye Anatomy 0.000 description 2
- NDJKXXJCMXVBJW-UHFFFAOYSA-N heptadecane Chemical compound CCCCCCCCCCCCCCCCC NDJKXXJCMXVBJW-UHFFFAOYSA-N 0.000 description 2
- 238000002386 leaching Methods 0.000 description 2
- 238000001819 mass spectrum Methods 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000002390 rotary evaporation Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 244000246386 Mentha pulegium Species 0.000 description 1
- 235000016257 Mentha pulegium Nutrition 0.000 description 1
- 235000004357 Mentha x piperita Nutrition 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- ZIJKGAXBCRWEOL-SAXBRCJISA-N Sucrose octaacetate Chemical compound CC(=O)O[C@H]1[C@H](OC(C)=O)[C@@H](COC(=O)C)O[C@@]1(COC(C)=O)O[C@@H]1[C@H](OC(C)=O)[C@@H](OC(C)=O)[C@H](OC(C)=O)[C@@H](COC(C)=O)O1 ZIJKGAXBCRWEOL-SAXBRCJISA-N 0.000 description 1
- 241000425037 Toona sinensis Species 0.000 description 1
- 239000001344 [(2S,3S,4R,5R)-4-acetyloxy-2,5-bis(acetyloxymethyl)-2-[(2R,3R,4S,5R,6R)-3,4,5-triacetyloxy-6-(acetyloxymethyl)oxan-2-yl]oxyoxolan-3-yl] acetate Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 235000001050 hortel pimenta Nutrition 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 238000010079 rubber tapping Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 229940013883 sucrose octaacetate Drugs 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000003828 vacuum filtration Methods 0.000 description 1
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- Extraction Or Liquid Replacement (AREA)
Abstract
The utility model belongs to the technical field of an extraction device, and particularly relates to a closed type crop leaf surface secreta cycle extraction device. The closed type crop leaf surface secreta cycle extraction device comprises a double-necked round bottom flask, a separating funnel provided with a suction filter mouth, a flat bottom funnel, a separating funnel, a tail connecting pipe and a condenser pipe which are sequentially connected from bottom to top, wherein the a branch mouth of the double-necked round bottom flask is connected with an outlet of the condenser pipe by a joint and a glass tube. After the closed type crop leaf surface secreta cycle extraction device is used, the defects that the existing extraction method is harm to the body of an operator and more volatile organic solvent is consumed can be overcome, the extraction rate is increased, and the error caused by artificial operation can be reduced. The closed type crop leaf surface secreta cycle extraction device is low in price and simple to operate, and is capable of efficiently and rapidly extracting the crop leaf surface secreta.
Description
Technical field
The utility model belongs to extraction element technical field, is specifically related to a kind of closed crop leaf surface secretion circulation extracting apparatus.
Background technology
At present, conventional blade face secretion extracting method is as follows: the area that needs first to record blade before lixiviate.When lixiviate, the methylene chloride of about 1000ml is poured in metal tray (clean anhydrous residual), clamp petiole base with tweezers blade full wafer is immersed to 2 seconds in dichloromethane solution, blade is proposed to solution 2 seconds, methylene chloride on blade is dropped back in dish, can be regarded as like this lixiviate once, every blade repeated lixiviate 4 times, in needing the methylene chloride on blade to drip back coil completely after last lixiviate.Methylene chloride all in pallet is transferred in the beaker of 2L, to the interior mark (if having high resolution gas chromatography-mass spectrum GC-MS to measure needs) that adds 1ml in beaker, fully mix, add 10g left and right anhydrous sodium sulfate, fully stir and suck moisture, be filtered in 1000 mL round-bottomed flasks with quantitative filter paper, filtrate decompression rotary evaporation, to 20ml left and right, is finally settled to 50ml volumetric flask.
The deficiency that existing extracting method exists: the 1) harm to human body.Methylene chloride is volatile, and 39.8 DEG C of boiling points, have toxic action to mouth and nose and eyes, suck excessive even can be carcinogenic.In the time containing methylene chloride by metal tray and carry out lixiviate, a large amount of methylene chloride can evaporate in air, operating personnel's preparation that must fully properly protect, even so, the methylene chloride evaporating still inevitably can touch people's skin, eyes and respiratory system.2) a large amount of reagent consumption or extraction rates is slow.Press when rotary evaporation taking out, more gaseous state methylene chloride condensation timely and directly being taken away by vacuum pump, causes loss and the contaminated environment of organic solvent; If do not take out pressure, be directly rotated evaporation, can to a certain degree save methylene chloride, but velocity of evaporation slows down greatly, affect test progress.
The flavor quality of blade surface secretion and crop is in close relations, as peppermint, Chinese toon, tobacco etc.At present, be the focus of Chinese scholars research to the research work of blade face secretion, and fundamental sum simple blade face secretion extraction work has been had no precedent improvement in research at home and abroad since the nineties in last century.Therefore, one efficiently, blade face secretion extraction element easily, significant in the correlative study of crop blade face secretion and development.
Utility model content
For overcoming prior art deficiency, the utility model object is to provide the closed crop leaf surface secretion circulation extracting apparatus of a kind of environmental protection, safety.
For achieving the above object, the utility model adopts following technical scheme:
A kind of closed crop leaf surface secretion circulation extracting apparatus, this device comprises the twoport round-bottomed flask connecting in turn from down to up, separating funnel, Buchner filter, separating funnel, tail adapter and the condenser pipe with suction filtration mouth, and a mouth of described twoport round-bottomed flask is connected with condenser pipe outlet with glass tube by joint.
Concrete, the described separating funnel lower end with suction filtration mouth is tightly connected by sheathed stopper and twoport round-bottomed flask; The top of described Buchner filter is tightly connected by diaphragm seal with holes or glass plate and separating funnel are set, and the aperture of described diaphragm seal or glass plate and the lower port of separating funnel match.
Further, described glass tube quantity can be more than two, between glass tube, between joint and glass tube, connects with rubber tube, and package unit uses also more convenient like this.
Compared to the prior art, the beneficial effects of the utility model:
1) avoided the infringement of toxic organic solvent to human body.2) volatile methylene chloride can condensation cycle utilization, saves cost and operation steps.3) distillation is a most time-consuming step, the utility model device has improved sample of every extraction in prior art and has distilled step once, keeps full-time distillation, and reduces the tapping amount of each lixiviate, accelerate distillation and reflux speed, saved the plenty of time.4) equipment price is cheap, simple to operate.
Brief description of the drawings
Fig. 1 is the structural representation of closed crop leaf described in the utility model surface secretion circulation extracting apparatus; In figure, the arrow at suction filtration mouth 13 places represents vacuum filtration direction, and other arrow represents methylene chloride loop direction.
Embodiment
Below in conjunction with accompanying drawing, the utility model is described in further details, but protection domain of the present utility model is not limited to this.
As shown in Figure 1, a kind of closed crop leaf surface secretion circulation extracting apparatus, this device comprises the twoport round-bottomed flask 1 connecting in turn from down to up, separating funnel 2, Buchner filter 3, separating funnel 4, tail adapter 5 and the condenser pipe 6 with suction filtration mouth 13, and a mouth of described twoport round-bottomed flask 1 is connected with condenser pipe 6 outlets with glass tube 9,10 by joint 7,8.Described separating funnel 2 lower ends with suction filtration mouth 13 are tightly connected by sheathed stopper 11 and twoport round-bottomed flask 1; The top of described Buchner filter 3 is tightly connected with separating funnel 4 by glass plate with holes 12 is set, and the lower port of the aperture of described glass plate 12 and separating funnel 4 matches.Between glass tube 9,10, between joint 7 and glass tube 9 and connect with rubber tube between joint 8 and glass tube 10.
The operation steps of the utility model device:
1, obtaining of blade
When sampling, blade packs into immediately in black plastic bag from plant takes off, and rapidly shading low temperature is preserved, and sampling finishes to take back rapidly laboratory and carry out the extraction of secretion afterwards.
2, the record of leaf area/weight
Amount after blade face secretion extracts is generally to calculate with unit area or unit weight.
Area: the area that needs first to record blade before lixiviate: sample blade is cut into the shape of known area size, and for subsequent use (large blade can carry out cutting in contrast with diameter 8-10cm filter paper; Vanelets adopts the reticle of length of side 1-2cm to carry out cutting).
Weight: record weight before extraction.
3, lixiviate
Tile in Buchner filter 3 filter paper of two corresponding sizes, then by cut or weigh after blade put into Buchner filter 3, add the methylene chloride of a small amount of anhydrous sodium sulfate and about 200mL simultaneously, cover rapidly glass with hole plate 12 with sealing.Blade soaked after 2-3 second, in the separating funnel 2 with suction filtration mouth 13, filtered, and opened the valve of the separating funnel 2 with suction filtration mouth 13 after suction filtration finishes, and filtrate entering is placed in the twoport round-bottomed flask 1 of 45 DEG C of water-baths, and started distillation.
Now, take away glass with hole plate 12, simultaneously to the methylene chloride that again adds rapidly about 200mL in Buchner filter 3.Blade repeated to soak after 2-3 second, in the separating funnel 2 with suction filtration mouth 13, again filter, suction filtration finishes rear filtrate and is first temporarily stored in the separating funnel 2 with suction filtration mouth 13, the distillation of filtrate in twoport round-bottomed flask 1 approaches the valve of opening the separating funnel 2 with suction filtration mouth 13 when complete, filtrate is entered in twoport round-bottomed flask 1 and proceed to distill.In the time that filtrate approaches evaporate to dryness again, in separating funnel 4, accumulate the methylene chloride that enough condensations are got off, now do not need in Buchner filter 3, to add new methylene chloride again, directly by the valve open of separating funnel 4, in Buchner filter 3, can obtain methylene chloride clean, that can reuse.
Repeat above step, until blade is leached 5-6 time.Need if any measuring, can enter the last time filtrate in twoport round-bottomed flask 1 while being evaporated to about 20ml, add the potpourri of 1mL sucrose octaacetate and n-heptadecane alcohol as interior mark, methylene chloride is settled to 50ml volumetric flask, can carry out with high performance liquid chromatography and mass spectrum the blade face secretion composition of Detection and Extraction gained.
While carrying out the extraction of next fresh sample, filter paper and the anhydrous sodium sulfate that only need in Buchner filter 3, more renew, add the sample for the treatment of lixiviate.
The utility model device all in closed state, has reduced because of the methylene chloride human body infringement causing of volatilizing in whole circulation leaching process.Meanwhile, a small amount of methylene chloride has been realized recycling, can be used for extracting the blade face secretion of multiple different samples.In whole leaching process, need the step of artificial main operation: the 1) valve of open and close separating funnel 4, separating funnel 2 with suction filtration mouth 13 in good time, and filter valve.2) the last extract of constant volume.3) change filter paper and sample.
The utility model device has made up harm and the volatile organic solvent consumption large defect of existing extracting method to operating personnel's health, and has accelerated extraction rate, has reduced the error of manual operation.This device low price, simple to operate, can extract safely, efficiently, rapidly crop blade face secretion.
Claims (3)
1. a closed crop leaf surface secretion circulation extracting apparatus, it is characterized in that, this device comprises the twoport round-bottomed flask connecting in turn from down to up, separating funnel, Buchner filter, separating funnel, tail adapter and the condenser pipe with suction filtration mouth, and a mouth of described twoport round-bottomed flask is connected with condenser pipe outlet with glass tube by joint.
2. closed crop leaf as claimed in claim 1 surface secretion circulation extracting apparatus, is characterized in that, the described separating funnel lower end with suction filtration mouth is tightly connected by sheathed stopper and twoport round-bottomed flask; The top of described Buchner filter is tightly connected by diaphragm seal with holes or glass plate and separating funnel are set, and the aperture of described diaphragm seal or glass plate and the lower port of separating funnel match.
3. closed crop leaf as claimed in claim 1 surface secretion circulation extracting apparatus, is characterized in that, described glass tube quantity is more than two, between glass tube, connect with rubber tube between joint and glass tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420105616.0U CN203798642U (en) | 2014-03-10 | 2014-03-10 | Closed type crop leaf surface secreta cycle extraction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420105616.0U CN203798642U (en) | 2014-03-10 | 2014-03-10 | Closed type crop leaf surface secreta cycle extraction device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203798642U true CN203798642U (en) | 2014-08-27 |
Family
ID=51380847
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420105616.0U Expired - Lifetime CN203798642U (en) | 2014-03-10 | 2014-03-10 | Closed type crop leaf surface secreta cycle extraction device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203798642U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103852361A (en) * | 2014-03-10 | 2014-06-11 | 河南中烟工业有限责任公司 | Closed device for circularly extracting crop leaf surface secreta |
-
2014
- 2014-03-10 CN CN201420105616.0U patent/CN203798642U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103852361A (en) * | 2014-03-10 | 2014-06-11 | 河南中烟工业有限责任公司 | Closed device for circularly extracting crop leaf surface secreta |
| CN103852361B (en) * | 2014-03-10 | 2016-08-24 | 河南中烟工业有限责任公司 | A kind of closed crop leaf surface secretions circulation extracting apparatus |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP02 | Change in the address of a patent holder |
Address after: 450000 Yulin South Road, Henan, Zheng Dong, No. 16 South Road, Zhengzhou Patentee after: CHINA TOBACCO HENAN INDUSTRIAL Co.,Ltd. Patentee after: HENAN AGRICULTURAL University Address before: 450000 Zhengzhou agricultural road, Henan, No. 29 Patentee before: CHINA TOBACCO HENAN INDUSTRIAL Co.,Ltd. Patentee before: HENAN AGRICULTURAL University |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20140827 |