CN203786006U - Flow Cytometry Laser Integrated Device - Google Patents
Flow Cytometry Laser Integrated Device Download PDFInfo
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- CN203786006U CN203786006U CN201420167117.4U CN201420167117U CN203786006U CN 203786006 U CN203786006 U CN 203786006U CN 201420167117 U CN201420167117 U CN 201420167117U CN 203786006 U CN203786006 U CN 203786006U
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- 238000000684 flow cytometry Methods 0.000 title abstract description 3
- 230000010354 integration Effects 0.000 claims abstract description 12
- 238000001514 detection method Methods 0.000 claims abstract description 10
- 239000000835 fiber Substances 0.000 claims description 13
- 230000005855 radiation Effects 0.000 abstract description 7
- 239000013307 optical fiber Substances 0.000 abstract description 5
- 238000007493 shaping process Methods 0.000 abstract description 5
- 230000005540 biological transmission Effects 0.000 abstract description 3
- 230000008878 coupling Effects 0.000 abstract description 3
- 238000010168 coupling process Methods 0.000 abstract description 3
- 238000005859 coupling reaction Methods 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 238000004806 packaging method and process Methods 0.000 abstract description 2
- 230000003287 optical effect Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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Abstract
Description
技术领域 technical field
本实用新型涉及医疗器械技术领域，具体为一种流式细胞仪激光整合装置。 The utility model relates to the technical field of medical equipment, in particular to a flow cytometer laser integration device.
背景技术 Background technique
流式细胞仪是集光子、电子学、流体力学、细胞化学、生物学、免疫学以及激光和计算机等多门学科技术为一体的先进科学技术设备，它被广泛应用于临床医学、细胞学、生物学、微生物学、制药学、生殖学等领域。是现代科学研究中的先进仪器之一，被誉为实验室的“CT”。它对快速直线流动状态中的单列细胞或生物颗粒进行逐个、多参数、快速的定性、定量分析或分选的技术，具有检测速度快、测量参数多、采集数据大、分析全面、分选纯度高、方法灵活等特点。 Flow cytometer is an advanced scientific and technical equipment integrating photons, electronics, fluid mechanics, cytochemistry, biology, immunology, laser and computer and other disciplines. It is widely used in clinical medicine, cytology, Biology, microbiology, pharmacy, reproductive science and other fields. It is one of the advanced instruments in modern scientific research, known as the "CT" of the laboratory. It performs one-by-one, multi-parameter, rapid qualitative and quantitative analysis or sorting technology on single cells or biological particles in a fast linear flow state. It has fast detection speed, multiple measurement parameters, large collection data, comprehensive analysis, and sorting purity. High, flexible methods and other characteristics.
现有的流式细胞仪激光光束整合系统是利用合光镜实现不同激光波长的透射与反射来完成不同波长激光的整合的。因此，现有的光束整合系统具有摆放精度要求高，调整困难，结构不灵活，不利于系统的模块化且容易受到外界噪声的干扰等缺点。 The existing flow cytometer laser beam integration system uses light combining mirrors to realize the transmission and reflection of different laser wavelengths to complete the integration of different wavelength lasers. Therefore, the existing beam integration system has the disadvantages of high placement accuracy, difficult adjustment, inflexible structure, unfavorable system modularization, and easy interference by external noise.
实用新型内容 Utility model content
本实用新型的目的是为了克服上述不足提供一种既易于调整结构，又具有较强抗干扰能力，防止由于激光器波长漂移引起的误差的流式细胞仪激光整合装置。 The purpose of this utility model is to provide a flow cytometer laser integration device that is easy to adjust the structure, has strong anti-interference ability, and prevents errors caused by laser wavelength drift in order to overcome the above-mentioned shortcomings.
为解决上述技术问题，实现上述目的，本实用新型通过如下技术方案实现： In order to solve the above-mentioned technical problems and achieve the above-mentioned purpose, the utility model is realized through the following technical solutions:
一种流式细胞仪激光整合装置，包括激光辐射单元和流动检测池，所述激光辐射单元和流动检测池之间沿激光传播方向依顺次设置有光纤耦合器和光束整形单元。 A flow cytometer laser integration device includes a laser radiation unit and a flow detection cell, and an optical fiber coupler and a beam shaping unit are sequentially arranged between the laser radiation unit and the flow detection cell along the laser propagation direction.
进一步的，所述激光辐射单元包括至少两个平行放置激光器。 Further, the laser radiation unit includes at least two parallel lasers.
优选的，所述光纤耦合器包括至少两个输入端以及一个输出端，所述输入端的个数与激光器的个数相同，所述每个激光器与每个输入端分别同轴设置。 Preferably, the fiber coupler includes at least two input ends and one output end, the number of the input ends is the same as the number of lasers, and each laser is coaxially arranged with each input end.
进一步的，所述光束整形单元由垂直于光轴方向且依顺次设置的准直透镜、第一柱面镜、第二柱面镜和聚焦透镜组成。 Further, the beam shaping unit is composed of a collimator lens, a first cylindrical mirror, a second cylindrical mirror and a focusing lens which are arranged in sequence perpendicular to the direction of the optical axis.
本实用新型具有以下有益效果： The utility model has the following beneficial effects:
采用多束激光光纤耦合装置，使系统结构易于调整，抗干扰能力强，能防止由于激光器波长漂移引起的误差，结构模块化、适合封装，便于生产。 The multi-beam laser fiber coupling device is used to make the system structure easy to adjust, strong anti-interference ability, and can prevent errors caused by laser wavelength drift. The structure is modular, suitable for packaging, and easy to produce.
附图说明 Description of drawings
图1为光纤耦合器示意图 Figure 1 is a schematic diagram of a fiber coupler
图2为激光整合系统示意图。 Figure 2 is a schematic diagram of the laser integration system.
具体实施方式 Detailed ways
下面结合附图及实施例进一步说明本实用新型。 Below in conjunction with accompanying drawing and embodiment further illustrate the utility model.
一种流式细胞仪激光整合装置如图2所示，包括激光器辐射单元100、光纤耦器200、光束整形单元300以及流动检测池400。其中激光辐射单元100包含有多个平行紧凑放置的激光器101，在激光辐射单元100沿激光传播方向设置的为光纤耦合器200。如图1所示，光纤耦器200包含有多个输入端201，以及一个输出端202，光纤耦器200的输入端201与激光器101一对一同轴心放置，并保持适当距离，以保证激光束能与输入端201的耦合透镜正交，使得激光束能耦合进入光纤耦合器200的输入端201。并在光纤内通过全反射传输到达输出端202，实现多束激光的输出变为一束激光的输出。位于光纤耦合器200后方，沿光束传播方向为光束整形单元300，它由准直透镜301、第一柱面镜302、第二柱面镜303和聚焦透镜304组成，准直透镜301置于光纤耦合器200输出端202后方，并垂直于光轴方向，将光纤耦器200输出端202输出的光线准直成平行光。第一柱面镜302和第二柱面镜303曲率不同且相互垂直放置于准直透镜301后方沿光轴方向，以保证光线垂直入射，第一柱面镜302和第二柱面镜303主要用于对光束X轴和Y轴方向进行不同程度的压缩，形成流式细胞检测所需的椭圆形光斑。第二柱面镜303后方沿光轴方向放置的平凸圆透镜作为聚焦透镜304，用于对光束进一步压缩形成适合细胞检测的最佳光斑照射至流动检测池400中的细胞样品流上。 A flow cytometer laser integration device is shown in FIG. 2 , which includes a laser radiation unit 100 , a fiber optic coupler 200 , a beam shaping unit 300 and a flow detection cell 400 . The laser radiation unit 100 includes a plurality of parallel and compact lasers 101 , and a fiber coupler 200 is arranged in the laser radiation unit 100 along the laser propagation direction. As shown in Figure 1, the fiber coupler 200 includes multiple input ports 201 and one output port 202. The input ports 201 of the fiber coupler 200 and the laser 101 are coaxially placed one-to-one, and an appropriate distance is kept to ensure that the laser The beam energy is orthogonal to the coupling lens of the input end 201 such that the laser beam energy is coupled into the input end 201 of the fiber coupler 200 . And it reaches the output end 202 through total reflection transmission in the optical fiber, so that the output of multiple laser beams becomes the output of one laser beam. Located behind the fiber coupler 200, along the beam propagation direction is the beam shaping unit 300, which consists of a collimating lens 301, a first cylindrical mirror 302, a second cylindrical mirror 303 and a focusing lens 304, and the collimating lens 301 is placed in the optical fiber Behind the output end 202 of the coupler 200 and perpendicular to the direction of the optical axis, the light output from the output end 202 of the fiber coupler 200 is collimated into parallel light. The first cylindrical mirror 302 and the second cylindrical mirror 303 have different curvatures and are vertically placed behind the collimating lens 301 along the optical axis direction to ensure that the light is vertically incident. The first cylindrical mirror 302 and the second cylindrical mirror 303 mainly It is used to compress the X-axis and Y-axis of the light beam to different degrees to form an elliptical spot required for flow cytometry detection. The plano-convex circular lens placed along the optical axis behind the second cylindrical lens 303 is used as a focusing lens 304 for further compressing the light beam to form an optimal light spot suitable for cell detection to irradiate the cell sample flow in the flow detection cell 400 .
上述实施例为本实用新型较佳的实施方式，但本实用新型的实施方式并不受上述实施例的限制，其他的任何未背离本实用新型的精神实质与原理下所作的改变、修饰、替代、组合、简化，均应为等效的置换方式，都包含在本实用新型的保护范围之内。 The above-mentioned embodiment is a preferred implementation mode of the present utility model, but the implementation mode of the present utility model is not limited by the above-mentioned embodiment, and any other changes, modifications and substitutions made without departing from the spirit and principle of the present utility model , combination, and simplification, all should be equivalent replacement methods, and are all included in the protection scope of the present utility model.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420167117.4U CN203786006U (en) | 2014-04-09 | 2014-04-09 | Flow Cytometry Laser Integrated Device |
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|---|---|---|---|
| CN201420167117.4U CN203786006U (en) | 2014-04-09 | 2014-04-09 | Flow Cytometry Laser Integrated Device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106226224A (en) * | 2016-08-18 | 2016-12-14 | 重庆玖润隆科技有限公司 | A kind of particle analyzer optical system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106226224A (en) * | 2016-08-18 | 2016-12-14 | 重庆玖润隆科技有限公司 | A kind of particle analyzer optical system |
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