CN203772841U - Centrifuge rotor for measuring and analyzing plant stem flow - Google Patents
Centrifuge rotor for measuring and analyzing plant stem flow Download PDFInfo
- Publication number
- CN203772841U CN203772841U CN201420187315.7U CN201420187315U CN203772841U CN 203772841 U CN203772841 U CN 203772841U CN 201420187315 U CN201420187315 U CN 201420187315U CN 203772841 U CN203772841 U CN 203772841U
- Authority
- CN
- China
- Prior art keywords
- rotor
- cuvette
- groove
- cover plate
- plant stem
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241000196324 Embryophyta Species 0.000 title claims abstract description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 27
- 238000005259 measurement Methods 0.000 claims description 16
- 238000000034 method Methods 0.000 abstract description 8
- 230000005499 meniscus Effects 0.000 description 7
- 239000002184 metal Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 208000005189 Embolism Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033558 biomineral tissue development Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000037039 plant physiology Effects 0.000 description 1
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- Centrifugal Separators (AREA)
Abstract
The utility model discloses a centrifuge rotor for measuring and analyzing plant stem flow. The centrifuge rotor comprises a rotor body, a rotor cover plate component and a handle, wherein the rotor body comprises a taper hole part and a disc type part; the taper hole part is used for being combined with a centrifuge in a driving manner; a groove for installing a cuvette and a plant stem section is formed in the disc type part; drainage holes are formed in two ends of the groove; the rotor cover plate component comprises a cover plate which is matched with the disc type part and used for covering the groove; a pipe, a water inlet opening and an observation hole are formed in the cover plate, and the pipe is used for injecting water in the cuvette; the water inlet opening is communicated with the pipe; the observation hole is used for observing the cuvette in the groove; the handle is arranged on the cover plate; a water inlet communicated with the water inlet opening in the cover plate is formed in the handle. By using the centrifuge rotor disclosed by the utility model, a centrifuge method for measuring a fragility curve is the fastest and the most effective method for obtaining plant drought resistance indexes (P50).
Description
Technical field
The utility model belongs to centrifuge rotor, is specifically related to a kind of centrifuge rotor of Measurement and analysis plant stem flow.
Background technology
Plant drought resistance research equipment is used for measuring plant xylem hydraulic conductivity and embolism, measures the anti-hole ability (P of plant xylem vessel
50), the relation of analysis plant physiology and plant moisture, is used for assessing Genes For Plant Tolerance arid ability, assessment Genes For Plant Tolerance frost resistivity etc.The plant drought resistance research equipment of application has stemflow measuring system at present, and it utilizes energy equilibrium sensor measurement plant stem flow; Plant hydraulic conductivity high voltage testing device, it utilizes supercharging technology to drive current by plant roots and stems sample, determines the water resistance/hydraulic conductivity of sample according to indexs such as the relations of water flow pressure and flow velocity.Hydro-extractor method is to measure plant drought resistance (P
50) the fastest and effective method, be therefore necessary to develop the centrifuge rotor of Measurement and analysis plant stem flow.
Utility model content
The purpose of this utility model is to provide the centrifuge rotor of good, the fireballing Measurement and analysis plant stem flow of a kind of measurement effect.
The technical scheme that realizes the employing of the utility model object is:
The centrifuge rotor of Measurement and analysis plant stem flow, comprises rotor block, rotor cover board component and handle; Described rotor block comprises that plate-like portion is provided with the groove for cuvette and axis section are installed for driving cone hole part and the plate-like portion of combination with hydro-extractor, and the two ends of groove are provided with osculum; Described rotor cover board component comprises with described plate-like portion and coordinates the cover plate that groove is hidden, and cover plate is provided with the inlet opening that is communicated with for the pipe to cuvette water filling, with pipe and for observing the observation port of cuvette in groove; Described handle is established on the cover board, and handle is provided with the water inlet being communicated with the inlet opening on cover plate.
The utility model also comprises two cuvettes, is respectively equipped with the aperture with its bottom with different distance on two cuvettes.
The osculum at described groove two ends radially arranges along plate-like portion.
Water inlet on described handle is funnel-shaped water inlet.
The internal diameter of described cuvette is 1cm, and the small pitch on one of them cuvette is from its bottom 14mm, and the small pitch on another cuvette is from its bottom 10mm.
The beneficial effects of the utility model:
Using the utility model centrifuge rotor, measure fragile curve with centrifuge method, is to obtain plant drought resistance index (P
50) the fastest and effective method.
Below in conjunction with accompanying drawing, the utility model is described further.
Brief description of the drawings
Fig. 1 is front view of the present utility model.
Embodiment
See Fig. 1, the centrifuge rotor of Measurement and analysis plant stem flow, comprises rotor block 1, rotor cover board component, handle 4 and two cuvettes 5,9; Described rotor block 1 comprises that plate-like portion 13 is provided with the groove 12 for cuvette 5,9 and axis section 8 are installed for driving cone hole part 10 and the plate-like portion 13 of combination with hydro-extractor, and the two ends of groove 12 are radially provided with osculum D, E along plate-like portion; Described rotor cover board component comprises with described plate-like portion 13 and coordinates the cover plate 2 that groove 12 is hidden, and cover plate 2 is provided with the inlet opening A that is communicated with for the metal tube 3 to cuvette 5,9 water fillings, with metal tube 3 and for observing observation port B, the C of the interior cuvette 5,9 of groove 12; Described handle 4 is established on the cover board, and handle 4 is provided with the funnel-shaped water inlet 11 being communicated with the inlet opening A on cover plate 2; The internal diameter of described cuvette 5,9 is 1cm, on two cuvettes 5,9, be respectively equipped with aperture a ＇, the b ＇ with its bottom with different distance, aperture a ＇ on one of them cuvette 5 is apart from its bottom 14 mm, and the aperture b ＇ on another cuvette 9 is apart from its bottom 10 mm.
Principle of work: the centrifuge rotor of Measurement and analysis plant stem flow is that one is measured axis section hydraulic conductivity (K by centrifugation
h) rotor.In hydro-extractor method, axis section 8 is placed in the central authorities of hydro-extractor turning axle, and axis section 8 two ends are immersed in water.In the time that axis section 8 is rotated with certain angular velocity (ω), centrifugal force makes the water in xylem bear certain negative pressure, rotating shaft place negative pressure absolute value T
0expression formula be
T
0?=0.5ρω
2R
2
In formula, ρ is the density of water, and R is the distance to turning axle.In theory, T
0reduce along with the increase of R, can infer accordingly the Tension Distribution state in stem section under centrifugal action.The rotating shaft place maximum (peak suction) that is stressed, reduces gradually along axial two ends, reduces to negative (malleation) to the axis section 8 end pressure that are immersed in water.
When the utility model uses, at the side of rotor block 1 plate-like portion 13, light source 6 is set corresponding with osculum D, the E of groove 12, microscope 7 is set above the plate-like portion 13 of rotor block 1 and aims at viewport B, the C in plate-like portion 13.Be about 27cm by one, diameter is that the axis section 8 of 5～7 mm is put into the groove 12 in rotor block 1 plate-like portion 13.Insert respectively in two cuvettes 5,9 at the two ends of axis section 8, is wherein placed in the aperture a ＇ of cuvette 5 of stem section 8 base portions apart from its bottom 14 mm, and the aperture b ＇ that is placed in the cuvette 9 at axis section 8 tops only has 10 mm apart from its bottom.In axis section 8 rotary courses, light source 6 can be presented to the picture of cuvette 5,9 middle water levels (concave meniscus) on the object lens of microscope 7 (or on automatic photography system).So under dark background, we just can see the white line that two bright concave meniscus are by microscope 7.By being installed on object lens, little scale just can measure two distances between concave meniscus.In the time that water flows to top from axis section 8 base portions, the water level that top cuvette is 9 li is constant, because water always flows out from the aperture b ＇ of 10 mm.So, to see for 7 times at microscope, higher concave meniscus seemingly moves towards fixing base portion concave meniscus.
In axis section 8 rotary courses, by metal tube on cover plate assembly 3 to water filling in cuvette 5.Because the aperture a ＇ on base portion cuvette 5 is apart from higher (14mm) at the bottom of ware, if to water filling in this cuvette 5, just can be between stem segment base portion and top the poor △ P of mineralization pressure:
△P=0.25ρω
2[R
2-(R-r)
2]
In formula, R is the distance of spindle central to top cuvette, and r is the water-head of two cuvettes.
Observe by microscope 7, the displacement of axis section 8 base portion concave meniscus be designated as to △ x, by the program arranging record concave meniscus move the required time △ t. of △ x so current move to the mass rate F(kg s at top from axis section 8 base portions
-1) expression formula be:
F=10
-3ρ(A
C-A
S)?△x/△t
A in formula
cfor the cross-sectional area of cuvette (is 1cm
2), A
sfor the cross-sectional area of stem segment base portion.
Stem section hydraulic conductivity K
hfor:
K
h?=FL/?△P
In formula, F is mass rate (kg s
-1), L is stem segment length (m), △ P is the pressure difference (MPa) at axis section 8 two ends.
By obtaining the data such as temperature, the angular velocity omega of rotor in hydro-extractor, computing machine can calculate T and △ P.Because the current under pressure state are metastable, when pressure, T is larger, and the possibility that bubble enters xylem vessel or test-tube baby is larger, once bubble enters, will cause xylem vessel to form hole, hinders current and passes through.Because axis section 8 has thousands of xylem vessels, and different xylem vessels is induced the required T difference in hole, if in increasing T gradually, by using centrifuge rotor and the hydro-extractor system of Measurement and analysis plant stem flow, constantly measure K
h, just can obtain the fragile curve being represented by hydraulic conductivity loss PLC and T relation.People's custom will cause 50% hydraulic conductivity (K
h) pressure (P in when loss
50) being used for characterizing the anti-hole ability of plant, this index is also used to characterize the drought resistance of plant.The exploitation of the centrifuge rotor of Measurement and analysis plant stem flow is to solve hydro-extractor method to measure plant drought resistance (P
50) key.
Claims (5)
1. a centrifuge rotor for Measurement and analysis plant stem flow, is characterized in that comprising rotor block, rotor cover board component and handle; Described rotor block comprises that plate-like portion is provided with the groove for cuvette and axis section are installed for driving cone hole part and the plate-like portion of combination with hydro-extractor, and the two ends of groove are provided with osculum; Described rotor cover board component comprises with described plate-like portion and coordinates the cover plate that groove is hidden, and cover plate is provided with the inlet opening that is communicated with for the pipe to cuvette water filling, with pipe and for observing the observation port of cuvette in groove; Described handle is established on the cover board, and handle is provided with the water inlet being communicated with the inlet opening on cover plate.
2. the centrifuge rotor of Measurement and analysis plant stem flow according to claim 1, is characterized in that also comprising two cuvettes, is respectively equipped with the aperture with its bottom with different distance on two cuvettes.
3. the centrifuge rotor of Measurement and analysis plant stem flow according to claim 2, is characterized in that the osculum at described groove two ends radially arranges along plate-like portion.
4. the centrifuge rotor of Measurement and analysis plant stem flow according to claim 3, is characterized in that the water inlet on described handle is funnel-shaped water inlet.
5. the centrifuge rotor of Measurement and analysis plant stem flow according to claim 4, the internal diameter that it is characterized in that described cuvette is 1cm, and the small pitch on one of them cuvette is from its bottom 14mm, and the small pitch on another cuvette is from its bottom 10mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420187315.7U CN203772841U (en) | 2014-04-18 | 2014-04-18 | Centrifuge rotor for measuring and analyzing plant stem flow |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420187315.7U CN203772841U (en) | 2014-04-18 | 2014-04-18 | Centrifuge rotor for measuring and analyzing plant stem flow |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203772841U true CN203772841U (en) | 2014-08-13 |
Family
ID=51290019
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420187315.7U Expired - Lifetime CN203772841U (en) | 2014-04-18 | 2014-04-18 | Centrifuge rotor for measuring and analyzing plant stem flow |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203772841U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107917866A (en) * | 2016-10-11 | 2018-04-17 | 湖南湘仪实验室仪器开发有限公司 | A kind of centrifuge method and apparatus for measuring plant hydraulic conductivity |
-
2014
- 2014-04-18 CN CN201420187315.7U patent/CN203772841U/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107917866A (en) * | 2016-10-11 | 2018-04-17 | 湖南湘仪实验室仪器开发有限公司 | A kind of centrifuge method and apparatus for measuring plant hydraulic conductivity |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20140813 |
|
| CX01 | Expiry of patent term |