CN203613194U - MIC (Minimum Inhibitory Concentration) multipoint inoculating system - Google Patents
MIC (Minimum Inhibitory Concentration) multipoint inoculating system Download PDFInfo
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- CN203613194U CN203613194U CN201320807192.8U CN201320807192U CN203613194U CN 203613194 U CN203613194 U CN 203613194U CN 201320807192 U CN201320807192 U CN 201320807192U CN 203613194 U CN203613194 U CN 203613194U
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- inoculating
- stepper
- guide rail
- motor
- rotating disk
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- 230000002401 inhibitory effect Effects 0.000 title abstract description 4
- 229920001817 Agar Polymers 0.000 claims abstract description 17
- 239000008272 agar Substances 0.000 claims abstract description 17
- 230000005540 biological transmission Effects 0.000 claims abstract description 17
- 230000008878 coupling Effects 0.000 claims abstract description 6
- 238000010168 coupling process Methods 0.000 claims abstract description 6
- 238000005859 coupling reaction Methods 0.000 claims abstract description 6
- 238000011081 inoculation Methods 0.000 claims description 50
- 239000003814 drug Substances 0.000 claims description 17
- 230000001580 bacterial effect Effects 0.000 claims description 15
- 230000033001 locomotion Effects 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 12
- 238000012360 testing method Methods 0.000 claims description 12
- 235000013372 meat Nutrition 0.000 claims description 11
- 235000014347 soups Nutrition 0.000 claims description 11
- 238000012546 transfer Methods 0.000 claims description 8
- 238000000034 method Methods 0.000 abstract description 16
- 230000008569 process Effects 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 4
- 241000894006 Bacteria Species 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 7
- 229940079593 drug Drugs 0.000 description 6
- 238000002815 broth microdilution Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000002814 agar dilution Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 230000001976 improved effect Effects 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 108010079058 casein hydrolysate Proteins 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
Images
Abstract
The utility model discloses an MIC (Minimum Inhibitory Concentration) multipoint inoculating system. The MIC multipoint inoculating system comprises a main body, wherein a rotating disk and a first photoelectric sensor are arranged on the main body; a first stepper motor and a second stepper motor are arranged in the main body; a transmission shaft is arranged between the second stepper motor and the rotating disk; the second stepper motor drives the rotating disk to horizontally rotate through the transmission shaft; the first stepper motor is connected with a first vertical screw guide rail through a coupling; the first vertical screw guide rail is connected with a transmission frame, and drives the transmission frame to vertically slide along the first vertical screw guide rail; inoculating needles and an inoculating frame are arranged on the transmission frame; a control panel is additionally arranged on the main body; a first 96-hole flat plate and an agar culture dish are arranged on the rotating disk. The MIC multipoint inoculating system realizes efficient and convenient completion of the inoculating task, relieves the workload of experimenters, and improves the inoculating effect; the sample adding volume and the number of inoculating holes can be set beforehand, and the inoculating condition can be directly reflected on a touch screen, so that the inoculating process is simple, quick and accurate.
Description
Technical field
The utility model relates to inoculation system field, relates in particular to a kind of MIC (minimum inhibition concentration) multiple spot inoculation system.
Background technology
The Surveillance of antibiotic resistance in bacterial isolates of antibiotics more and more obtains national attention, and its resistance trend and Drug-resistance to accurate grasp bacterium antagonism medicine, instructs clinical rational drug use to have great importance.In mensuration MIC value process, microbionation is numerous and diverse various to the process procedures on the medicine of different concns, be in the past manual operations, with inoculation side or liquid-transfering gun application of sample inoculation one by one, greatly increase the weight of experimenter's workload, complicated operation effect of inoculation is poor, can not guarantee the accuracy of inoculum size and the circulation ratio of each effect of inoculation.
Utility model content
The utility model provides a kind of MIC multiple spot inoculation system, and this inoculation system has reduced experimenter's workload, has improved effect of inoculation, described below:
A kind of MIC multiple spot inoculation system, described multiple spot inoculation system comprises: body, on described body, be provided with rotating disk and the first photo-sensor, in described body, be provided with the first stepper-motor and the second stepper-motor, between described the second stepper-motor and described rotating disk, be provided with transmission shaft, described the second stepper-motor drives described rotating disk left rotation and right rotation by described transmission shaft; Described the first stepper-motor connects the first vertical screw guide rail by shaft coupling; Described the first vertical screw guide rail connection for transmission frame, and drive described gear frame to slide up and down along described the first vertical screw guide rail; On described gear frame, be provided with inoculating needle and inoculation frame; On described body, be also provided with control panel, on described rotating disk, be provided with the one 96 hole flat board and agar culture dish.
Further, in order to meet the multiple needs in practical application, on described body, be also provided with foot-operated socket; On described body, be provided with overall plastic casing.
A kind of MIC multiple spot inoculation system, described multiple spot inoculation system comprises: body, in described body, be provided with the 3rd stepper-motor, described the 3rd stepper-motor connects the second vertical screw guide rail, on described the second vertical screw guide rail, be provided with liquid-transfering gun vertical movement control enclosure, described liquid-transfering gun vertical movement control enclosure electrical connection liquid-transfering gun control enclosure, is provided with eight passage liquid-transfering guns in described liquid-transfering gun control enclosure, on described liquid-transfering gun control enclosure, be provided with liquid transfer gun head; In described body, be also provided with the 4th stepper-motor, described the 4th stepper-motor connects horizontal screw bolt guide rail, on described horizontal screw bolt guide rail, is provided with the second photo-sensor; On described body, be also provided with control panel, rifle head box, withdraw of the needle groove, meat soup groove, medicine test tube, bacterial classification test tube and the 2 96 hole flat board.
Further, in order to meet the multiple needs in practical application, described control panel is touch control screen.
The beneficial effect of the technical scheme that the utility model provides is: this MIC multiple spot inoculation system has been realized the inoculation task that efficiently completes easily, has reduced experimenter's workload, has improved effect of inoculation; Multi-work-station high precision inoculating needle and multiple tracks liquid-transfering gun simultaneously, has guaranteed the consistence circulation ratio of inoculating; This provides data reliably to being related to the rational use of drug of life, the decision-making of science administration, has stronger practicality.Application of sample volume and inoculation hole figure place all can set in advance and on touch-screen, intuitively reflect inoculation situation, make seeded process simple, quick and accurate.
Accompanying drawing explanation
Fig. 1 is agar dilution inoculation system structural representation;
Fig. 2 is the front view of agar dilution inoculation system;
Fig. 3 is the schematic diagram of the one 96 hole flat board, agar culture dish;
Fig. 4 is broth dilution method inoculation system structural representation;
Fig. 5 is the main diagram of broth dilution method inoculation system;
Fig. 6 is the schematic perspective view of broth dilution method inoculation system;
Fig. 7 is the schematic diagram of touch control screen.
In accompanying drawing, the list of parts of each label representative is as follows:
A: body; 1: rotating disk;
2: the first photo-sensors; 3: transmission shaft;
4: inoculating needle; 5: inoculation frame;
6: gear frame; 7: the first vertical screw guide rails;
8: shaft coupling; 9: foot-operated socket;
10: the first stepper-motors; 11: the second stepper-motors;
12: overall plastic casing; 13: control panel;
14: the one 96 hole flat boards; 15: agar culture dish;
B: body; 16: the three stepper-motors;
17: the second vertical screw guide rails; 18: liquid-transfering gun vertical movement control enclosure;
19: liquid-transfering gun control enclosure; 20: eight passage liquid-transfering guns;
21: liquid transfer gun head; 22: the four stepper-motors;
23: horizontal screw bolt guide rail; 24: the second photo-sensors;
25: control panel; 26: rifle head box;
27: withdraw of the needle groove; 28: meat soup groove;
29: medicine test tube; 30: bacterial classification test tube;
31: the 2 96 hole flat boards.
Embodiment
For making the purpose of this utility model, technical scheme and advantage clearer, below the utility model embodiment is described in further detail.
A kind of MIC multiple spot inoculation system, referring to Fig. 1, Fig. 2 and Fig. 3, comprise: body A, on body A, be provided with rotating disk 1 and the first photo-sensor 2, in body A, be provided with the first stepper-motor 10 and the second stepper-motor 11, between the second stepper-motor 11 and rotating disk 1, be provided with transmission shaft 3, the second stepper-motors 11 and drive rotating disk 1 left rotation and right rotation by transmission shaft 3; The first stepper-motor 10 connects the first vertical screw guide rail 7 by shaft coupling 8; The first vertical screw guide rail 7 connection for transmission framves 6, and drive gear frame 6 to slide up and down along the first vertical screw guide rail 7; On gear frame 6, be provided with inoculating needle 4 and inoculation frame 5; On body A, be also provided with control panel 13, on rotating disk 1, be provided with the one 96 hole dull and stereotyped 14 and agar culture dish 15.
When specific implementation, in order to facilitate the access of practical application power supply, on body A, be also provided with foot-operated socket 9.
Further, on body A, also facilitate the needs in practical application by being provided with overall plastic casing 12.
When practical application, the second stepper-motor 11 drives rotating disk 1 left rotation and right rotation by transmission shaft 3, and the accurate rotation that the first photo-sensor 2 is being controlled rotating disk 1, realizes positioning function.The first stepper-motor 10 drives the first vertical screw guide rail 7 to drive gear frame 6, inoculation frame 5 and inoculating needle 4 vertical movements by shaft coupling 8.When rotating disk 1 side-to-side movement, by changing different concns agar culture dish 15, the bacterium in the one 96 hole flat board 14 can be inoculated into above agar culture dish 15 by inoculating needle 4, can suspend at any time and open instrument by foot-operated socket 9, discharge successively both hands.
The working process of inoculation system is described by concrete agar dilution drug sensitive experiment below: add bacterial classification to the one 96 hole flat board 14 in advance, the medicine of different concns and agar mix the agar culture dish 15 of making different pharmaceutical concentration.The one 96 hole preparing dull and stereotyped 14 and agar culture dish 15 are placed on rotating disk 1, start operation, the second stepper-motor 11 drives rotating disk 1 that the one 96 hole flat board 14 is rotated to central position, the first stepper-motor 11 drives the first vertical screw guide rail 7, and then drive inoculating needle 4 to move downward bacterial classification in bonding the one 96 hole flat board 14, inoculating needle 4 connects bacterial classification, move upward, rotating disk 1 forwards center to the agar culture dish 15 preparing subsequently, inoculating needle 4 moves downward again, the bacterial classification of just receiving inoculating needle 4 is received in agar culture dish 15, after finished, the one 96 hole flat board 14 turns back to again center, now can replace the agar culture dish 15 that connects bacterial classification, repeat such motion, finally bacterial classification is inoculated in the agar culture dish 15 of different concns, cultivate connecting the agar culture dish 15 of planting again, finally observe minimum inhibitory concentration.Inoculating needle 4 uses through the stainless steel of special processing and makes, and due to special structure design and special processing, when having eliminated the hydrophobicity of inoculating needle and having prevented sampling, bacterium drop falls.Adopt bacterium liquid measure accurate, circulation ratio is high, and the detection means that reliability is high is adopted bacterium amount accurate, guarantees the successfully inoculation on agar culture dish 15 of all inoculating needles 4.Unique adjustable speed rotating sample pallet can be realized automatic location, and without operation, only need to change sample can complete inoculation.Traditional-handwork method single can only be inoculated one, and inoculum size is inconsistent, poor repeatability.60, this instrument one time, repeatability and consistence are high, have shortened greatly the operating time, and efficiency is provided, and have met the requirement of laboratory automation.The utility model makes drug sensitive experiment easy and simple to handle, and experimental result accuracy and circulation ratio are high, realize rapid detection, improves treatment capacity, is applicable to batch experiment.
In sum, this inoculation system is moved up and down by the inoculation parts of line slideway transmission control, and 60 (or 24) inoculating needles that cooperation can Z axis degree of freedom are alternately stained with liquid inoculation between bacterium liquid and cultivation face, have completed continuously multiple spot seeded process one time.Through the inoculating needle of hydrophobic treatment and the relative position of orifice plate and the face of cultivation, lock its original position by the light control sensor below rolling disc.Accuracy and the circulation ratio of seeded process are guaranteed.
A kind of MIC multiple spot inoculation system, referring to Fig. 4, Fig. 5, Fig. 6 and Fig. 7, comprise: body B, in body B, be provided with the 3rd stepper-motor 16, the 3rd stepper-motor 16 connects on the second vertical screw guide rail 17, the second vertical screw guide rails 17 and is provided with liquid-transfering gun vertical movement control enclosure 18, and liquid-transfering gun vertical movement control enclosure 18 is electrically connected liquid-transfering gun control enclosure 19, in liquid-transfering gun control enclosure 19, be provided with eight passage liquid-transfering guns 20, on liquid-transfering gun control enclosure 19, be provided with liquid transfer gun head 21; In body B, be also provided with the 4th stepper-motor 22, the four stepper-motors 22 and connect horizontal screw bolt guide rail 23, on horizontal screw bolt guide rail 23, be provided with the second photo-sensor 24; On body B, be also provided with control panel 25, rifle head box 26, withdraw of the needle groove 27, meat soup groove 28, medicine test tube 29, bacterial classification test tube the 30 and the 2 96 hole flat board 31.
Further, control panel 25 can also, for touch control screen, meet the multiple needs in practical application.
When practical application, the 4th stepper-motor 22 drives 23 tangential movements of horizontal screw bolt guide rail, the 3rd stepper-motor 16 drives the second vertical screw guide rail 17 to move up and down, and controls eight passage liquid-transfering guns 20, gets meat soup, medicine and bacterium liquid and is assigned on the 2 96 hole flat board 31 with different concentration gradients.
The working process of inoculation system is described by concrete broth dilution method drug sensitive experiment below: the 3rd stepper-motor 16 is controlled the second upwards vertical movement of vertical screw guide rail 17, and then drives liquid-transfering gun control enclosure 19 and eight passage liquid-transfering guns 20 to move upward; Then the 4th stepper-motor 22 level of control screw rod guide rail 23 levels are to left movement, and wherein the second photo-sensor 24 is accurately controlled eight passage liquid-transfering guns 20 and moved to high order end, aims at the first volley of rifle fire head in rifle head box 26; Eight passage liquid-transfering guns 20 move downward vertically and automatically go up liquid transfer gun head 21, upwards vertical movement of liquid transfer gun head 21, then eight passage liquid-transfering guns 2 move right to meat soup groove 28 upper end positions, move downward and draw MH(caseinhydrolysate) meat soup, and then MH meat soup 50 μ L are assigned in the 2nd round to the 12 rounds of the 2 96 hole flat board 31; Eight passage liquid-transfering guns 20 are moved back into medicine test tube 29 upper ends again gets the denseest antibacterials 100 μ L and is added to the first round of the 2 96 hole flat board 31, taking out 50 μ L is added to the second round and mixes, taking out 50 μ L from the second round is again added to the 3rd round and mixes ... until the 11 round, the 50 μ L that take out from the 11 round discard (every hole remains 50 μ L); Get again MH meat soup and in every hole, add 100 μ L; Eight passage liquid-transfering guns 20 return bacterial classification test tube 30 and get original bacterial concentration 1-2x108CFU/mL(0.5 Maxwell standard 0.08-0.1 absorbancy) 50 μ L(are equivalent to get 5x106CFU and are added in every hole); Last eight passage liquid-transfering guns 20 move to withdraw of the needle groove 27 rifle head are decorporated; So just finally prepare the 2 96 hole flat board 31 of different gradient concentrations, then cultivate bacterium colony, observe minimum inhibitory concentration.A whole set of experiment flow, from getting liquid transfer gun head 21 to distributing meat soup, medicine and bacterial classification, then to the withdraw of the needle, be all fully automatic operation, processing speed is fast, within 80 seconds, can complete a 2 96 hole flat board 31, without personnel's operation, saves time and avoids polluting.Control panel 25 has been realized simulation effect of inoculation and has been shown, the hole preparing shows in real time as figure seven (the first and second rows prepare).The utility model makes drug sensitive experiment easy and simple to handle, and experimental result accuracy and circulation ratio are high, realize rapid detection, improves treatment capacity, is applicable to batch experiment.
In sum, at horizontal rail/vertical guideway eight passage liquid-transfering guns that vertical/horizontal moves respectively, complete liquid transfer gun head in the imbibition of meat soup groove at touch control display control, successively in medicine test tube and the sampling of bacterial classification test tube, in the process of the dull and stereotyped cloth bacterium in 96 holes.This inoculation system can be according to program palikinesia, once completes the accurate cloth bacterium inoculation in each road.Its application of sample volume and inoculation hole figure place all can set in advance and on touch-screen, intuitively reflect inoculation situation, make seeded process simple, quick and accurate.
The utility model embodiment to the model of each device except do specified otherwise, the model of other devices does not limit, and all can as long as can complete the device of above-mentioned functions.
It will be appreciated by those skilled in the art that accompanying drawing is the schematic diagram of a preferred embodiment, above-mentioned the utility model embodiment sequence number, just to describing, does not represent the quality of embodiment.
The foregoing is only preferred embodiment of the present utility model, not in order to limit the utility model, all within spirit of the present utility model and principle, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection domain of the present utility model.
Claims (5)
1. a MIC multiple spot inoculation system, described multiple spot inoculation system comprises: body (A), it is characterized in that, on described body (A), be provided with rotating disk (1) and the first photo-sensor (2), in described body (A), be provided with the first stepper-motor (10) and the second stepper-motor (11), between described the second stepper-motor (11) and described rotating disk (1), be provided with transmission shaft (3), described the second stepper-motor (11) drives described rotating disk (1) left rotation and right rotation by described transmission shaft (3); Described the first stepper-motor (10) connects the first vertical screw guide rail (7) by shaft coupling (8); Described the first vertical screw guide rail (7) connection for transmission frame (6), and drive described gear frame (6) to slide up and down along described the first vertical screw guide rail (7); On described gear frame (6), be provided with inoculating needle (4) and inoculation frame (5); On described body (A), be also provided with control panel (13), on described rotating disk (1), be provided with the one 96 hole flat board (14) and agar culture dish (15).
2. a kind of MIC multiple spot inoculation system according to claim 1, is characterized in that, is also provided with foot-operated socket (9) on described body (A).
3. a kind of MIC multiple spot inoculation system according to claim 1, is characterized in that, is provided with overall plastic casing (12) on described body (A).
4. a MIC multiple spot inoculation system, described multiple spot inoculation system comprises: body (B), it is characterized in that, in described body (B), be provided with the 3rd stepper-motor (16), described the 3rd stepper-motor (16) connects the second vertical screw guide rail (17), on described the second vertical screw guide rail (17), be provided with liquid-transfering gun vertical movement control enclosure (18), described liquid-transfering gun vertical movement control enclosure (18) electrical connection liquid-transfering gun control enclosure (19), in described liquid-transfering gun control enclosure (19), be provided with eight passage liquid-transfering guns (20), on described liquid-transfering gun control enclosure (19), be provided with liquid transfer gun head (21), in described body (B), be also provided with the 4th stepper-motor (22), described the 4th stepper-motor (22) connects horizontal screw bolt guide rail (23), is provided with the second photo-sensor (24) on described horizontal screw bolt guide rail (23), on described body (B), be also provided with control panel (25), rifle head box (26), withdraw of the needle groove (27), meat soup groove (28), medicine test tube (29), bacterial classification test tube (30) and the 2 96 hole flat board (31).
5. a kind of MIC multiple spot inoculation system according to claim 4, is characterized in that, described control panel (25) is touch control screen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320807192.8U CN203613194U (en) | 2013-12-09 | 2013-12-09 | MIC (Minimum Inhibitory Concentration) multipoint inoculating system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320807192.8U CN203613194U (en) | 2013-12-09 | 2013-12-09 | MIC (Minimum Inhibitory Concentration) multipoint inoculating system |
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| CN203613194U true CN203613194U (en) | 2014-05-28 |
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| CN201320807192.8U Expired - Lifetime CN203613194U (en) | 2013-12-09 | 2013-12-09 | MIC (Minimum Inhibitory Concentration) multipoint inoculating system |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104342363A (en) * | 2014-10-23 | 2015-02-11 | 沈阳师范大学 | Quick mass inoculator |
| CN106955752A (en) * | 2017-04-25 | 2017-07-18 | 波顿（上海）生物技术有限公司 | A kind of perforated plate sampling height adjustor |
| CN107024909A (en) * | 2017-06-01 | 2017-08-08 | 沈阳建筑大学 | It is a kind of that instrument numerical control device is inoculated with based on the PLC medical multiple spots controlled |
-
2013
- 2013-12-09 CN CN201320807192.8U patent/CN203613194U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104342363A (en) * | 2014-10-23 | 2015-02-11 | 沈阳师范大学 | Quick mass inoculator |
| CN106955752A (en) * | 2017-04-25 | 2017-07-18 | 波顿（上海）生物技术有限公司 | A kind of perforated plate sampling height adjustor |
| CN107024909A (en) * | 2017-06-01 | 2017-08-08 | 沈阳建筑大学 | It is a kind of that instrument numerical control device is inoculated with based on the PLC medical multiple spots controlled |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
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| CX01 | Expiry of patent term |
Granted publication date: 20140528 |