CN203530315U - Automatic purifying and separating system - Google Patents
Automatic purifying and separating system Download PDFInfo
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- CN203530315U CN203530315U CN201320624964.4U CN201320624964U CN203530315U CN 203530315 U CN203530315 U CN 203530315U CN 201320624964 U CN201320624964 U CN 201320624964U CN 203530315 U CN203530315 U CN 203530315U
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- slide block
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- purifies
- worktable
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Abstract
The utility model relates to the technical field of purification and separation of biomolecules, and particularly relates to an automatic purifying and separating system capable of carrying out massive and automatic operation. The system comprises a work bench, a magnetic suction device and a filling device, wherein the magnetic suction device and the filling device are arranged at the side of the work bench; injection and extraction of various reagents can be automatically finished through the filling device; probing and removing of a magnetic bar on a magnetic rack can be automatically finished by the magnetic suction device, so that the orderly automatic operation of splitting, combining, cleaning and eluting can be achieved by matching controllable rotation of the work bench, and batch operation can be achieved by combining with a porous plate.
Description
Technical field
The utility model relates to the purifies and separates technical field of biomolecules, relate in particular to a kind of can mass, the automatic purifies and separates system of automated job.
Background technology
Nucleotide extracting and purifying method for biomolecules generally needs four key steps:
1, cracking: preparation one is loaded with the centrifuge tube of lysate, will treat that manipulating cells is placed in centrifuge tube and carries out cracking, makes cell rupture with released dna;
2, combination: employing magnetic bead etc., as solid phase carrier, are placed in centrifuge tube by solid phase carrier, and the DNA discharging is combined with solid phase carrier;
3, clean: the magnet stand (as magnetism stick etc.) that is set with sheath is insinuated into and in centrifuge tube, adsorbs magnetic bead, and then the suction unit such as hand-held negative pressure rifle is extracted out in conjunction with liquid, then magnetic bead is cleaned, and then extracts scavenging solution out;
4, wash-out: inject elutriant in centrifuge tube, then remove the magnet stand in sheath, magnetic bead departs from sheath and falls into the elutriant in centrifuge tube, makes DNA depart from magnetic bead by modes such as heating and sneaks into the DNA that obtains purifying in elutriant.
Above-mentioned existing working method, links all must be by operating personnel's manual operations, and efficiency is low, and cannot realize batched operation.
Therefore,, for above-mentioned defect, the utility model provides a kind of automatization purification system.
Utility model content
(1) technical problem that will solve
The purpose of this utility model is for a kind of automatic purifies and separates system is provided, to realize automatization, the mass operation of purifying biomolecules.
(2) technical scheme
For solving the problems of the technologies described above, the utility model provides a kind of automatic purifies and separates system, and this system comprises worktable and the magnetic attracting device and the filling apparatus that are arranged on described worktable side, wherein:
Described worktable is circular platform, on described worktable, be provided with a plurality of orifice plates, a plurality of described orifice plates are radial around the center of circle of described worktable and evenly arrange, described worktable can rotate around the center of circle of described worktable, makes corresponding orifice plate correspond respectively to described magnetic attracting device and described filling apparatus;
Described magnetic attracting device comprise be positioned at corresponding orifice plate top sheath frame, be positioned at the magnet stand of described sheath frame top and for driving vertically mobile hoisting appliance of described sheath frame and described magnet stand.
Wherein, each bar magnet of described magnet stand moves down in the corresponding sheath that can be inserted into described sheath frame, and the described sheath that is fitted with described bar magnet can move down in the hole that stretches to corresponding orifice plate.
Wherein, described filling apparatus comprises filling gun, reagent trough and slide block mechanism, described filling gun is positioned at the upper side of described slide block mechanism, described reagent trough be positioned at described slide block mechanism lower side, described slide block mechanism can order about described filling gun along vertically and horizontal direction move.
Wherein, described slide block mechanism comprises filling column and transverse arm, described reagent trough is packed in the bottom of described filling column, on described filling column, vertical slide block is installed, described transverse arm is higher than described orifice plate and be the level of state and be arranged on described filling column by vertical slide block, on described transverse arm, being provided with can be along the transverse slider of horizontal slip, and described filling gun is arranged on described transverse slider.
Wherein, the rifle head of described filling gun is set to multiple-head type rifle head, and one in a plurality of described orifice plates is set to rack-like structures, for placing standby multiple-head type rifle head.
Wherein, described reagent trough has a plurality of hole slots, for separating the different reagent of splendid attire.
Wherein, on described filling gun, be provided with level sensor, for monitoring the degree of depth probeing in described reagent trough and injecting the reagent height in described orifice plate; On described filling gun, be provided with rifle head sensor, on the orifice plate of described rack-like structures, find rifle head to be installed when changing rifle head.
Wherein, described transverse slider and described vertical slide block are driven by corresponding motor respectively, and described in each, motor is connected on a main control computer.
Wherein, described hoisting appliance comprises magnet stand post, on described magnet stand post, be provided with can be vertically mobile magnet stand slide block and sheath slide block, described magnet stand is arranged on described magnet stand slide block, described sheath frame is arranged on described sheath slide block; Described magnet stand slide block and described sheath slide block are driven by corresponding motor respectively, and described in each, motor is connected on described main control computer.
Wherein, at least one is provided with hot-plate for holding the bottom of the orifice plate of reagent.
Wherein, described worktable orders about it by corresponding motor and rotates, and described motor is connected on described main control computer, and is provided with brush web member at described worktable circle centre position, and the supply lead of described brush web member and described hot-plate is slidably connected.
(3) beneficial effect
Technique scheme tool of the present utility model has the following advantages: the automatic purifies and separates system that the utility model provides, by filling apparatus, can automatically complete injection and the extraction of each reagent, by magnetic attracting device, can automatically complete probeing into and shifting out of bar magnet on magnet stand, the controllable rotating of cooperating platform, and then can realize the sequential automation operation of cracking, combination, cleaning and wash-out, and in conjunction with the setting of porous plate, can realize mass operation.
Accompanying drawing explanation
Fig. 1 is the three-dimensional structure diagram of the automatic purifies and separates system of the utility model;
Fig. 2 is the perspective view of the utility model orifice plate.
In figure, 1: anchor; 2: worktable; 3: magnetic attracting device; 4: filling apparatus; 5: orifice plate; 6: hot-plate; 7: sheath frame; 8: magnet stand; 9: magnet stand post; 10: filling gun; 11: reagent trough; 12: filling column; 13: transverse arm; 14: transverse slider; 15: vertical slide block.
Embodiment
Below in conjunction with drawings and Examples, embodiment of the present utility model is described in further detail.Following examples are used for illustrating the utility model, but are not used for limiting scope of the present utility model.
As shown in Figure 1, a kind of automatic purifies and separates system, this system comprises worktable 2, magnetic attracting device 3 and the filling apparatus 4 being arranged on anchor 1, magnetic attracting device 3 and filling apparatus 4 are positioned at the side of circular table 2 can be corresponding with a plurality of orifice plates 5 on worktable 2.
Described worktable 2 is circular platform, on worktable 2, be provided with a plurality of orifice plates 5, can correspond respectively to magnetic attracting device 3 and filling apparatus 4, a plurality of plates are radial around the center of circle of worktable and evenly arrange, worktable 2 can rotate around the center of circle, make corresponding orifice plate 5 correspond respectively to magnetic attracting device 3 and filling apparatus 4, so that in the magnet stand probe hole of magnetic attracting device 3 and be convenient to filling apparatus 4 and automatically complete and extract and inject reagent.Wherein, described orifice plate can be placed on worktable in activity, after once using, can be removed, and on corresponding position, places new orifice plate, meets the requirement of test accuracy.
Orifice plate 5 in this embodiment comprises that the orifice plate 5 of 7 cabinet types that carry out purifying operation for splendid attire reagent and 1 are for placing the orifice plate 5 of the rack-like structures of standby rifle head, the orifice plate 5(of the cabinet type of a pair of symmetry arrangement is for the orifice plate 5 of splendid attire reagent therein) bottom is provided with hot-plate 6, for elutriant being heated in wash-out link.Certainly, the orifice plate 5 of a cabinet type that carries out purifying operation for splendid attire reagent and 1 also can be set on worktable for placing the orifice plate 5 of the rack-like structures of standby rifle head, at this, for splendid attire reagent, carry out the orifice plate 5 of the cabinet type of purifying operation, can hold the required all ingredients of test.Worktable 2 orders about its rotation by corresponding motor, described motor is connected on described main control computer, in order to prevent that the rotation of worktable 2 is wound around or the supply lead of the disconnected hot-plate 6 of strand, preferably, at worktable 2 circle centre positions, be provided with the brush web member of connecting feeder ear (as civil power), above-mentioned supply lead is slidably connected on brush web member.
As shown in Figure 2, each orifice plate 5 preferably adopts 8 * 12 holes, has 12 rounds, and 8 holes of every row, to realize mass operation.Obviously, for being applicable to different production and product demand, can also be set to the models such as orifice plate 5 such as 4 * 6 holes.
Continue as shown in Figure 1, magnetic attracting device 3 comprises sheath frame 7, magnet stand 8 and for ordering about sheath frame 7 and magnet stand 8 mobile hoisting appliance vertically respectively, hoisting appliance comprises magnet stand post 9, on magnet stand post 9, be provided with can be vertically mobile magnet stand slide block and sheath slide block, magnet stand 8 is arranged on magnet stand slide block, sheath frame 7 is arranged on sheath slide block, magnet stand 8 is positioned at the top of sheath frame 7, each bar magnet of magnet stand 8 moves down in the respective sheath that can be inserted into sheath frame 7, the sheath that is fitted with magnet stand can move down in the hole that stretches to corresponding orifice plate 5, can adsorb magnetic bead, mix in conjunction with liquid, wherein, magnet stand slide block and sheath slide block are driven by corresponding motor respectively, magnet stand slide block and sheath slide block can slide simultaneously, also can slide separately, each motor is connected on described main control computer.
Described filling apparatus 4 comprises filling gun 10, reagent trough 11 and slide block mechanism.Filling gun 10 is preferably negative pressure filling gun, slide block mechanism comprises filling column 12 and transverse arm 13, the bottom sides of filling column 12 is fixed with reagent trough 11, on filling column 12, the vertical slide block 15 that can vertically slide is installed, transverse arm 13 higher than orifice plate 5 and be the level of state by vertical slide block 15 be arranged on filling column 12 on, on transverse arm 13, be provided with and can, along the transverse slider 14 of horizontal slip, filling gun 10 be installed on transverse slider 14.Wherein, reagent trough 11 has a plurality of hole slots, for separating the different reagent of splendid attire; On filling gun 10, be provided with level sensor, for monitoring the degree of depth probeing in reagent trough 11 and injecting the reagent height in orifice plate 5, thereby can draw exactly reagent; On described filling gun 10, be provided with rifle head sensor, on the orifice plate of described rack-like structures, find rifle head to be installed when changing rifle head; Transverse slider 14 and vertical slide block 15 are driven by corresponding motor respectively, and each motor is connected on a main control computer.
Like this, on filling column 12, vertical slide block is set, on transverse arm 13, transverse slider is set, by vertical slide block 15 and transverse slider 14, can make filling gun 10 move along vertical and horizontal direction, specifically, make filling gun 10 in reagent trough 11, extract reagent and move up after certain altitude, laterally move to corresponding orifice plate 5 tops, and move down injection reagent to the hole of orifice plate 5, and after also can making filling gun 10 draw reagent from the hole of orifice plate, move up, transfering reagent to other holes of orifice plate or transfering reagent to the hole of other orifice plates.
In sum, by magnetic attracting device 3, realizing magnet stand automatization probes into and shifts out in the pipe (or hole) on orifice plate 5, by filling apparatus 4, realizing filling gun 10 automatizations draws and injects reagent to the pipe (or hole) of orifice plate 5, the controlledization rotation of cooperating platform 2, can realize periodically carrying out in proper order of biomolecules cracking, combination, cleaning and wash-out four processes, and then realize the automated job of purifying biomolecules.
Below be only preferred implementation of the present utility model; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of the utility model know-why; can also make some improvement and modification, these improve and modification also should be considered as protection domain of the present utility model.
Claims (10)
1. an automatic purifies and separates system, is characterized in that, this system comprises worktable (2) and be arranged on magnetic attracting device (3) and the filling apparatus (4) of described worktable (2) side, wherein:
Described worktable (2) is circular platform, on described worktable (2), be provided with a plurality of orifice plates (5), a plurality of described orifice plates (5) are radial around the center of circle of described worktable (2) and evenly arrange, described worktable (2) can rotate around the center of circle of described worktable (2), makes corresponding orifice plate (5) correspond respectively to described magnetic attracting device (3) and described filling apparatus (4);
Described magnetic attracting device (3) comprise be positioned at corresponding orifice plate (5) top sheath frame (7), be positioned at the magnet stand (8) of described sheath frame (7) top and for driving vertically mobile hoisting appliance of described sheath frame (7) and described magnet stand (8).
2. automatic purifies and separates system according to claim 1, it is characterized in that, each bar magnet of described magnet stand (8) moves down in the corresponding sheath that can be inserted into described sheath frame (7), and the described sheath that is fitted with described bar magnet can move down in the hole that stretches to corresponding orifice plate (5).
3. automatic purifies and separates system according to claim 2, it is characterized in that, described filling apparatus (4) comprises filling gun (10), reagent trough (11) and slide block mechanism, described filling gun (10) is positioned at the upper side of described slide block mechanism, described reagent trough (11) is positioned at the lower side of described slide block mechanism, and described slide block mechanism can order about described filling gun (10) and move along vertical and horizontal direction.
4. automatic purifies and separates system according to claim 3, it is characterized in that, described slide block mechanism comprises filling column (12) and transverse arm (13), described reagent trough (11) is packed in the bottom of described filling column (12), vertical slide block (15) is installed on described filling column (12), described transverse arm (13) is higher than described orifice plate (5) and be the level of state and be arranged on described filling column (12) by vertical slide block (15), on described transverse arm (13), being provided with can be along the transverse slider (14) of horizontal slip, described filling gun (10) is arranged on described transverse slider (14).
5. automatic purifies and separates system according to claim 4, is characterized in that, the rifle head of described filling gun (10) is set to multiple-head type rifle head, and one in a plurality of described orifice plates (5) is set to rack-like structures, for placing standby multiple-head type rifle head.
6. automatic purifies and separates system according to claim 5, is characterized in that, described filling gun is provided with level sensor on (10), for monitoring, probes into the degree of depth in described reagent trough (11) and injects the reagent height in described orifice plate (5); On described filling gun (10), be provided with rifle head sensor, on the orifice plate of described rack-like structures, find rifle head to be installed when changing rifle head.
7. automatic purifies and separates system according to claim 6, is characterized in that, described transverse slider (14) and described vertical slide block (15) are driven by corresponding motor respectively, and described in each, motor is connected on a main control computer.
8. automatic purifies and separates system according to claim 7, it is characterized in that, described hoisting appliance comprises magnet stand post (9), on described magnet stand post (9), be provided with can be vertically mobile magnet stand slide block and sheath slide block, described magnet stand (8) is arranged on described magnet stand slide block, and described sheath frame (7) is arranged on described sheath slide block; Described magnet stand slide block and described sheath slide block are driven by corresponding motor respectively, and described in each, motor is connected on described main control computer.
9. automatic purifies and separates system according to claim 7, is characterized in that, at least one is provided with hot-plate (6) for holding the bottom of the orifice plate (5) of reagent.
10. automatic purifies and separates system according to claim 9, it is characterized in that, described worktable (2) orders about its rotation by corresponding motor, described motor is connected on described main control computer, and at described worktable (2) circle centre position, be provided with brush web member, the supply lead of described brush web member and described hot-plate (6) is slidably connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320624964.4U CN203530315U (en) | 2013-10-10 | 2013-10-10 | Automatic purifying and separating system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320624964.4U CN203530315U (en) | 2013-10-10 | 2013-10-10 | Automatic purifying and separating system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203530315U true CN203530315U (en) | 2014-04-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320624964.4U Withdrawn - After Issue CN203530315U (en) | 2013-10-10 | 2013-10-10 | Automatic purifying and separating system |
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| Country | Link |
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| CN (1) | CN203530315U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103484363A (en) * | 2013-10-10 | 2014-01-01 | 北京中纳海润生物科技有限公司 | Automatic purification and separation system |
-
2013
- 2013-10-10 CN CN201320624964.4U patent/CN203530315U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103484363A (en) * | 2013-10-10 | 2014-01-01 | 北京中纳海润生物科技有限公司 | Automatic purification and separation system |
| CN103484363B (en) * | 2013-10-10 | 2015-01-07 | 北京中纳海润生物科技有限公司 | Automatic purification and separation system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20140409 Effective date of abandoning: 20150107 |
|
| RGAV | Abandon patent right to avoid regrant |