CN203519546U - System for rapidly detecting pesticide residues by using electrochemical biosensor - Google Patents
System for rapidly detecting pesticide residues by using electrochemical biosensor Download PDFInfo
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- CN203519546U CN203519546U CN201320577352.4U CN201320577352U CN203519546U CN 203519546 U CN203519546 U CN 203519546U CN 201320577352 U CN201320577352 U CN 201320577352U CN 203519546 U CN203519546 U CN 203519546U
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Abstract
The utility model relates to an electrochemical detecting device for food safety and particularly relates to a system for rapidly detecting pesticide residues by using an electrochemical biosensor. The system for rapidly detecting pesticide residues by using the electrochemical biosensor is characterized in that an instrument support is hinged with an analytical instrument through a data exchange serial port; a strip biosensor is clamped at the bottom end of the instrument support and is provided with an instrumental analysis port; the instrumental analysis port is matched with a microflow dialysis membrane; and the microflow dialysis membrane is hinged with the instrumental analysis port through an instrument lifting guide rail. By using the electrochemical detecting device, the problems of complex operation for detecting food additives and toxic and harmful substances and long detection time are solved, and a microfluid mixer chip which is easy to prepare, simple and convenient in testing operation, high in sensitivity, strong in specificity, short in response time and capable of quantitatively detecting the contents of food additives and the toxic and harmful substances is provided.
Description
Technical field
The utility model relates to a kind of Electrochemical Detection food security device, specifically a kind of rapid detection system of electrochemica biological sensor residues of pesticides.
Background technology
Residues of pesticides are that agricultural chemicals is used and in the rear period, is not decomposed and residues in the general name of trace pesticide substance, toxic metabolite, degradation product and impurity in biosome, cutting, soil, water body, atmosphere.Be applied to the agricultural chemicals on crop, wherein a part is attached on crop, and a part is scattered in the environment such as soil, large G&W, and the part in the remaining agricultural chemicals of environment can be absorbed by plants again.Remains of pesticide directly passes through fruit or water, atmosphere arrives in people, animal body, or finally passes to people, animal by environment, food chain.The residual stripper of agriculture can degradation water fruit vegetable surface residues of pesticides.
And food security refers to that food is nontoxic, harmless, meet the nutritional requirement that should have, health is not caused to any acute, subacute or chronic hazard.According to the definition of the World Health Organization (WHO), food security is " public health problem of poisonous and harmful substances to Health Impact in food ".Food security is also to inquire into guarantee food hygiene and edible safety in the processes such as food processing, storage, sale, reduces disease risk, takes precautions against interdisciplinary fields of food poisoning." bread is the staff of life ", the closest diet problem of specialty research daily life relation, its effect can not be underestimated.Job operation, process and device used in Food Science and Engineering research food industry production, she is the design basis of food production process and equipment, relates to each subjects such as chemistry, physics, agronomy, biological chemistry, microbiology, chemical engineering, Biochemical Engineering, mechanical engineering, human nutrition and food hygiene, environmental improvement and engineering.An importance of Food Science and Engineering is introduce and use chemical engineering unit operation, and concurrent spread becomes food engineering unit operations, thereby promotes food industry to the future development of extensive, serialization and robotization.The same chemical engineering of development of Food Science and Engineering, bioengineering are closely related, and its developing direction is that new packing means and equipment improve Food Packaging technology, improve food preservation performance and shelf life; Improve boiling technology, aseptic packaging technology; Study rational energy saver to reduce the cost of frozen food; The optimization of food engineering unit operations, robotization and computer application.Although Food Science and Engineering are young technology-oriented disciplines, Dan Ta modern society becomes the outstanding feature of economic development, civilization degree raising already.Following development trend is to use for reference the development result of gene technology, develops that nutrition is more abundant, more delicious taste, more scientific food of shelf-life.
Therefore detecting fast and effectively adjuvant not up to standard in food and poisonous and hazardous material is the current important development problem for whole food security industry.
Summary of the invention
The purpose of this utility model is to provide a kind of rapid detection system of electrochemica biological sensor residues of pesticides, thereby fills up in existing market some drawbacks for Electrochemical Detection food additives, poisonous and harmful substance.
The utility model solves its above-mentioned technical matters and adopts following technical scheme: a kind of rapid detection system of electrochemica biological sensor residues of pesticides, it is mainly configured with: analytical instrument, instrument support, exchanges data serial ports, instrument riser guide, instrumental analysis port, strip biology sensor, miniflow dialysis membrane, strip biology sensor port, sensor chip, sample sampler, nanometer dialysis plate, sample suction inlet, negative electrode termination, anode termination, comparison liquid permeable membrane, sample comparison liquid hybrid films, micro flow chip, chip substrate, between described instrument support and analytical instrument, by exchanges data serial ports, be hinged, the clamping of instrument support bottom has strip biology sensor, and strip biology sensor is provided with instrumental analysis port, instrumental analysis port and miniflow dialysis membrane match, between miniflow dialysis membrane and instrumental analysis port, by instrument riser guide, be linked,
Described its top of strip biology sensor is provided with strip biology sensor port, and bottom is provided with sample sampler, and its inside is provided with sensor chip;
Described sensor chip is comprised of nanometer dialysis plate compound chip substrate; Between described sensor chip and chip substrate, accompany successively and compare liquid permeable membrane, sample is compared liquid hybrid films;
Described chip substrate is provided with two electrode tips, is respectively negative electrode termination, anode termination; Negative electrode termination, anode termination are accessed on micro flow chip respectively.
Above-mentioned instrument support is provided with data-out port.
Above-mentioned strip biology sensor is the disposable material of throwing.
The beneficial effects of the utility model: solved food additives, poisonous and harmful substance are detected to complicated operation, and need the long problem of spended time, the microfluid mixer chip that provide that a kind of easy preparation and test operation are simple and convenient, highly sensitive, high specificity, response time are short, can quantitatively detect food additives, poisonous and harmful substance content, has realized the direct-detection to food additives, poisonous and harmful substance content.The utility model is of great significance in food industry and the equal tool of field of environment protection.
Accompanying drawing explanation
Fig. 1 is the rapid detection system one-piece construction schematic diagram of a kind of electrochemica biological sensor residues of pesticides of the utility model.
Fig. 2 is the structural representation of the rapid detection system strip biology sensor of a kind of electrochemica biological sensor residues of pesticides of the utility model.
Fig. 3 is the structural representation of the rapid detection system sensor chip of a kind of electrochemica biological sensor residues of pesticides of the utility model.
Fig. 4 is the electric signal/time chart after rapid detection system embodiment 1 sample of a kind of electrochemica biological sensor residues of pesticides of the utility model injects.
Fig. 5 is the electric signal/time chart after rapid detection system embodiment 2 samples of a kind of electrochemica biological sensor residues of pesticides of the utility model inject.
Fig. 6 is the electric signal/time chart after rapid detection system embodiment 3 samples of a kind of electrochemica biological sensor residues of pesticides of the utility model inject.
1-analytical instrument in figure, 2-instrument support, 3-exchanges data serial ports, 4-instrument riser guide, 5-instrumental analysis port, 6-strip biology sensor, 7-miniflow dialysis membrane, 8-strip biology sensor port, 9-sensor chip, 10-sample sampler, 11-nanometer dialysis plate, 12-sample suction inlet, 13-negative electrode termination, 14-anode termination, 15-comparison liquid permeable membrane, 16-sample comparison liquid hybrid films, 17-micro flow chip, 18-chip substrate.
Embodiment
Below in conjunction with accompanying drawing 1-6, embodiment of the present utility model is made a detailed explanation.
Embodiment: a kind of rapid detection system of electrochemica biological sensor residues of pesticides, it is mainly configured with: analytical instrument 1, instrument support 2, exchanges data serial ports 3, instrument riser guide 4, instrumental analysis port 5, strip biology sensor 6, miniflow dialysis membrane 7, strip biology sensor port 8, sensor chip 9, sample sampler 10, nanometer dialysis plate 11, sample suction inlet 12, negative electrode termination 13, anode termination 14, comparison liquid permeable membrane 15, sample comparison liquid hybrid films 16, micro flow chip 17, chip substrate 18, between described instrument support 2 and analytical instrument 1, by exchanges data serial ports 3, be hinged, instrument support 2 bottom clampings have strip biology sensor 6, and strip biology sensor 6 is provided with instrumental analysis port 5, instrumental analysis port 5 matches with miniflow dialysis membrane 7, between miniflow dialysis membrane 7 and instrumental analysis port 5, by instrument riser guide 4, be linked,
Described strip biology sensor 6 its tops are provided with strip biology sensor port 8, and bottom is provided with sample sampler 10, and its inside is provided with sensor chip 9;
Described sensor chip 9 is comprised of nanometer dialysis plate 11 compound chip substrates 18; Between described sensor chip 9 and chip substrate 18, accompany successively and compare liquid permeable membrane 15, sample is compared liquid hybrid films 16;
Described chip substrate 18 is provided with two electrode tips, is respectively negative electrode termination 13, anode termination 14; Negative electrode termination 13, anode termination 14 are accessed respectively on micro flow chip 17.
Described instrument support 2 is provided with data-out port.
Described strip biology sensor 6 is the disposable material of throwing.
For the comparison liquid application testing in comparison liquid permeable membrane 15, make a detailed explanation below:
Comparison liquid application testing embodiment 1:
One of the method is, in the materials such as poly terephthalic acid second two vinegar or poly-carbonic acid vinegar, sneaking in advance interfacial agent etc. has the chemical substance of interfacial activity effect and forms comparison liquid permeable membrane 15, improve thus the wellability of nanometer dialysis plate 11 sidewalls, the blood of taking can be introduced rapidly and reliably in nanometer dialysis plate 11 from sample suction inlet 12.As sneaking into aforementioned comparison liquid permeable membrane 15 materials, be expected the kind (as the classification of hydrophilic group) of the interfacial agent of above-mentioned effect, can enumerate anionic interfacial agents such as causing sour basin, sulfonate, phosphoric acid vinegar basin, the 1st grade of amine basin, the 2nd grade of amine basin, 3rd level amine basin, the 4th grade are pressed the cationic interfacial agents such as basin, the zwitterionic surfactant such as amino acid pattern or betaine type, and the non-ionic surfactant such as polyethylene glycol type or multivalence ethanol-type etc.In addition,, as the sample comparison liquid hybrid films 16 that can sneak into aforementioned interfacial agent, except above-mentioned points, can enumerate benzene dicarboxylic acid butanediol vinegar while gathering, polyetheramine, Polyvinylchloride, the sub-ethene of polychlorostyrene, polyimide, nylon etc.If use this 1st example as above, owing to sneaking into interfacial agent etc. in the material itself by sample comparison liquid hybrid films 16 and have the chemical substance of interfacial activity effect, the sidewall of the nanometer dialysis plate 11 of introducing towards blood, the part towards nanometer dialysis plate 11 of namely sample comparison liquid hybrid films 16, there is water wettability, so can make the wellability of the sidewall of nanometer dialysis plate 11 improve, the blood of taking from sample suction inlet 12 is introduced rapidly and reliably in nanometer dialysis plate 11.In addition meanwhile, the interfacial agent layer on reagent layer can be removed, the manufacture process of biology sensor can be simplified.Moreover, interfacial agent is sneaked into and become the blood that the insulativity base material of sample comparison liquid hybrid films 16 produces and attract facilitation effect, at the addition of interfacial agent, be that 0.01 % by weight just can fully be awared when above.Fig. 4 has shown the linear graph of the change in electric of interior its biology sensor of unit interval in sample injection process.
Comparison liquid application testing embodiment 2:
Although in the 1st example, interfacial agent is sneaked into the material of aforementioned sample comparison liquid hybrid films 16 itself, make thus the part towards nanometer dialysis plate 11 of this sample comparison liquid hybrid films 16 there is water wettability, but in this 2nd example, the interfacial agent of enumerating be coated on the 1st example on becoming the insulativity films such as poly terephthalic acid second two vinegar of base material of sample comparison liquid hybrid films 16 or poly-carbonic acid vinegar in, or the resin on laminating surface with water wettability polar group covers aforementioned dielectric film, so that the part towards nanometer dialysis plate 11 of sample comparison liquid hybrid films 16 has water wettability.Here, as the resin that has aforementioned water wettability polar group, can enumerate propylene class, polyethylene kind, poly-atmosphere vinegar class etc.In addition, on the surface of insulativity base material that becomes sample comparison liquid hybrid films 16, form the occasion of hydrophilic coating film, in the material of aforementioned substrates, be not limited to the insulativity films such as above-mentioned poly terephthalic acid second two vinegar or poly-carbonic acid vinegar, also can use polybutylene terephthalate vinegar, poly-phthalein amine, Polyvinylchloride, the sub-ethene of polychlorostyrene, poly-dead drunk imines, nylon etc.Moreover, with organic admire compounds, polyethyleneimine: aminated compounds, isocyanate compounds etc., to becoming poly terephthalic acid second two vinegar or poly-the breaking on the insulativity films such as vinegar of the base material of sample comparison liquid hybrid films 16, carry out subbing processing, also the water wettability that improves thus aforementioned nanometer dialysis plate 11 sidewalls, can make wellability improve.If use this 2nd example as above, due on the insulativity film of base material that becomes sample comparison liquid hybrid films 16, coating interfacial agent, or on laminating surface, there is the resin of water wettability polar group and cover the surface that aforementioned sample is compared liquid hybrid films 16, the sidewall of the nanometer dialysis plate 11 of introducing towards blood, the part towards nanometer dialysis plate 11 of namely sample comparison liquid hybrid films 16 is made has water wettability, so can make the wellability of nanometer dialysis plate 11 sidewalls improve, the blood of taking from sample suction inlet 12 is introduced rapidly and reliably in nanometer dialysis plate 11.In addition meanwhile, the interfacial agent layer on reagent layer can be removed, the manufacture process of biology sensor can be simplified.
Moreover, although be coated on the thickness of the interfacial agent on the insulativity film that becomes aforementioned sample comparison liquid hybrid films 16 base materials, if or the thickness of the resin bed with water wettability polar group of lamination surpasses the effect that tens dusts just can be awared the attraction of promotion blood, but for aforementioned effect is continued for a long time, preferably more than hundreds of dust.Fig. 5 has shown the linear graph of the change in electric of interior its biology sensor of unit interval in sample injection process.
Comparison liquid application testing embodiment 3:
Although in the 1st example, interfacial agent is sneaked into the material of aforementioned sample comparison liquid hybrid films 16 itself, make thus aforementioned sample comparison liquid hybrid films 16 there is water wettability towards the part of nanometer dialysis plate 11, but in this 3rd example, chemical surface treatment, processing are implemented in surface to the sample comparison liquid hybrid films 16 towards nanometer dialysis plate 11, make sample comparison liquid hybrid films 16 have water wettability towards the part of nanometer dialysis plate 11.As the concrete grammar that aforementioned sample comparison liquid hybrid films 16 is carried out to chemical surface treatment, processing towards the part of nanometer dialysis plate 11, can enumerate the Corona discharge Treatment or the glow discharge processing that for example with plasma discharge, are treated to representative, on the surface of the sample comparison liquid hybrid films 16 towards nanometer dialysis plate 11, form the water wettability function bases such as Hong base, township's base, light base, the material surface chemical modification that makes aforementioned sample comparison liquid hybrid films 16, improves surface wettability.In addition, as the material that can carry out chemically treated sample comparison liquid hybrid films 16, except above-mentioned that poly terephthalic acid second two vinegar or poly-carbonic acid vinegar, can also use polybutylene terephthalate vinegar, poly-dead drunk amine, Polyvinylchloride, the sub-ethene of polychlorostyrene, poly-dead drunk imines, nylon etc.If use this 3rd example as above, because the sample of the cavity volume 12 introducing towards blood is compared on the surface of liquid hybrid films 16, by implementing chemical surface treatment, processing and chemical modification, make aforementioned sample comparison liquid hybrid films 16 there is water wettability towards the part of nanometer dialysis plate 11, so can make the wellability of nanometer dialysis plate 11 sidewalls improve, the blood of taking from sample suction inlet 12 is introduced rapidly and reliably in nanometer dialysis plate 11.In addition meanwhile, the interfacial agent layer on reagent layer can be removed, the manufacture process of biology sensor can be simplified.Moreover, as the processing that makes surface texture chemical modification, except plasma discharge is processed, also have coupling reaction processing, ozone treatment, UV treatment etc., in the occasion by any disposal route, can obtain aforementioned same effect.Fig. 6 has shown the linear graph of the change in electric of interior its biology sensor of unit interval in sample injection process.
Claims (3)
1. the rapid detection system of electrochemica biological sensor residues of pesticides, it is mainly configured with: analytical instrument (1), instrument support (2), exchanges data serial ports (3), instrument riser guide (4), instrumental analysis port (5), strip biology sensor (6), miniflow dialysis membrane (7), strip biology sensor port (8), sensor chip (9), sample sampler (10), nanometer dialysis plate (11), sample suction inlet (12), negative electrode termination (13), anode termination (14), comparison liquid permeable membrane (15), sample comparison liquid hybrid films (16), micro flow chip (17), chip substrate (18), it is characterized in that: between instrument support (2) and analytical instrument (1), by exchanges data serial ports (3), be hinged, the clamping of instrument support (2) bottom has strip biology sensor (6), and strip biology sensor (6) is provided with instrumental analysis port (5), instrumental analysis port (5) matches with miniflow dialysis membrane (7), between miniflow dialysis membrane (7) and instrumental analysis port (5), by instrument riser guide (4), be linked,
Described its top of strip biology sensor (6) is provided with strip biology sensor port (8), and bottom is provided with sample sampler (10), and its inside is provided with sensor chip (9);
Described sensor chip (9) is comprised of nanometer dialysis plate (11) compound chip substrate (18); Between described sensor chip (9) and chip substrate (18), accompany successively comparison liquid permeable membrane (15), sample comparison liquid hybrid films (16);
Described chip substrate (18) is provided with two electrode tips, is respectively negative electrode termination (13), anode termination (14); Negative electrode termination (13), anode termination (14) are accessed respectively on micro flow chip (17).
2. the rapid detection system of a kind of electrochemica biological sensor residues of pesticides according to claim 1, is characterized in that described instrument support (2) is provided with data-out port.
3. the rapid detection system of a kind of electrochemica biological sensor residues of pesticides according to claim 1, is characterized in that described strip biology sensor (6) is the disposable material of throwing.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201320577352.4U CN203519546U (en) | 2013-09-17 | 2013-09-17 | System for rapidly detecting pesticide residues by using electrochemical biosensor |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201320577352.4U CN203519546U (en) | 2013-09-17 | 2013-09-17 | System for rapidly detecting pesticide residues by using electrochemical biosensor |
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| CN203519546U true CN203519546U (en) | 2014-04-02 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111979117A (en) * | 2020-08-27 | 2020-11-24 | 苏州联睿微半导体科技有限公司 | Bacterium detection device based on food |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111979117A (en) * | 2020-08-27 | 2020-11-24 | 苏州联睿微半导体科技有限公司 | Bacterium detection device based on food |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140402 Termination date: 20140917 |
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| EXPY | Termination of patent right or utility model |