CN203451536U - Mesh division plate culture medium - Google Patents
Mesh division plate culture medium Download PDFInfo
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- CN203451536U CN203451536U CN201320466479.9U CN201320466479U CN203451536U CN 203451536 U CN203451536 U CN 203451536U CN 201320466479 U CN201320466479 U CN 201320466479U CN 203451536 U CN203451536 U CN 203451536U
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- culture medium
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Abstract
The utility model relates to the field of in vitro diagnostic reagents, and particularly discloses a mesh division plate culture medium. The mesh division plate culture medium comprises a plate culture vessel, and is characterized in that the culture vessel is internally provided with a plurality of partition plates which can equally divide the culture vessel, the culture vessel is equally divided into a plurality of spaces through the partition plates, and the sub spaces are filled with different culture mediums. The mesh division plate culture medium provided by the utility model has the advantages that the structure is simple, the use is convenient, the time and labor are saved, the cost is also saved, numbering and comparing are convenient, the culture and verification are combined into a whole, the accuracy of a check result is improved, and the mesh division plate culture medium is suitable for wide popularization and application.
Description
(1) technical field
The utility model relates to external diagnosis reagent field, particularly a kind of lattice plate culture medium.
(2) background technology
Substratum is by artificial means, the microorganism growth such as bacterium is bred to needed nutritive substance and be mixed in proportion, and regulates pH value, the application mix thing that autoclaving forms.The cultivation of microorganism and isolation technique be unable to do without substratum, by the technology of solid medium separation and purification microorganism, are the basic premises that carries out microbiological research.Suitable substratum can make microorganism ramp breeding, improves the separation rate to pathogenic bacteria, is beneficial to the Clinics and Practices of clinical disease.
At present, microorganism plate culture medium is of a great variety, is widely used.In Clinical Laboratory process, because Specimen origin is different, check object is different, need to inoculate several different plate culture mediums simultaneously.And testing laboratory use more in culture dish, only have a kind of substratum single substratum, make a sample need to inoculate several different types of plate culture mediums simultaneously, complex operation not only, waste material, but also easily cause pathogenic bacterium undetected, incurs loss through delay check process.
(3) summary of the invention
The utility model, in order to make up the deficiencies in the prior art, provides a kind of lattice plate culture medium simple in structure, easy to use.
The utility model is achieved by the following technical solution:
A lattice plate culture medium, comprises dull and stereotyped culture dish, it is characterized in that: in described culture dish, be provided with several dividing plates of being divided equally, culture dish is divided into the different substratum of splendid attire in ，Fen space, several minute space by dividing plate.
The utility model adopts dividing plate that culture dish is divided into several minute space, according to the difference of application target, the substratum of filling multiple different sorts and purposes in minute space, during use, different lattices region in the dull and stereotyped culture dish of same, the same sample of streak inoculation simultaneously after cultivation, is convenient to observe assay simultaneously.
More excellent scheme of the present utility model is:
Described culture dish is the transparent culture dish of aseptic plastic.
Described dividing plate is one, and culture dish is divided into two minutes spaces.
Described dividing plate is three, and culture dish is divided into three minutes spaces.
By culture dish, by baffle design, be two lattices or vertically dividing equally check into three culture dish, a sample can be inoculated on two or three different substratum, to reach the check object of separated various pathogenic bacterium simultaneously; The substratum of different sorts and purposes is incorporated in a culture dish, and user only need lack time inoculation can complete check object, the limitation while having overcome existing single plate culture medium use.
The utility model is simple in structure, easy to use, time saving and energy saving, cost-saving, is convenient to numbering and contrasts, and integrates cultivation, identifies, has improved the accuracy of assay, is suitable for wide popularization and application.
(4) accompanying drawing explanation
Below in conjunction with accompanying drawing, the utility model is further described.
Fig. 1 is the structural representation of the utility model list dividing plate state;
Fig. 2 is the structural representation of the utility model three dividing plate states.
In figure, 1 culture dish, 2 dividing plates, 3 minutes spaces.
(5) embodiment
Accompanying drawing is a kind of specific embodiment of the present utility model.This embodiment comprises dull and stereotyped culture dish 1, is provided with several dividing plates 2 of being divided equally in described culture dish 1, and culture dish 1 is divided into several minute space 3 by dividing plate 2, minute different substratum of the interior splendid attire in space 3; Described culture dish 1 is the transparent culture dish of aseptic plastic; Described dividing plate 2 is one, and culture dish 1 is divided into two minutes spaces 3; Described dividing plate 2 is three, and culture dish 1 is divided into three minutes spaces 3.
Embodiment 1:
As shown in Figure 1, a kind of two lattice plate culture mediums, comprise the aseptic plate of two lattices, Colombia nutrient agar matrix A, maconkey agar medium matrix B.
Colombia's blood agar culture-medium main component has peptone, sodium-chlor, beef heart infusion, Zulkovsky starch, agar etc., for the cultivation to the overcritical bacterium of nutrition.Maconkey agar substratum main component has peptone, lactose, sodium-chlor, cholate, toluylene red, agar etc., for separating of with differentiate intestinal bacteria.Hemolytic reaction does not occur in blood agar substratum according to pathogen enterobacteria, and in Mai Kangkai substratum, there is the reactions such as the colour developing of different bacterium colony and cholate precipitation in different entero-bacte, can make preliminary evaluation to pathogen enterobacteria thus.
During use, by sample inoculation line in Colombia's agar/maconkey agar lattice of same flat board simultaneously, put in constant temperature incubator and cultivate, observations.There is not hemolytic reaction in pathogen enterobacteria, in maconkey agar substratum, streptococcus faecium presents red bacterium colony in Colombia's blood agar culture-medium, there is no cholate precipitation.Bacillus coli present red bacterium colony and around substratum redden, the visible cholate of streak inoculation precipitation.Salmonella typhimurium colony colourless, precipitates without cholate.Therefore,, by the cultivation of lattice plate culture medium is observed, can tentatively determine the concrete pathogen enterobacteria kind that whether contains pathogen enterobacteria and contain in sample.
As shown in Figure 1, a kind of two lattice plate culture mediums, comprise the aseptic plate of two lattices, eosin methylene blue agar medium matrix A, SS nutrient agar matrix B.
Eosin methylene blue agar medium main component has peptone, lactose, dipotassium hydrogen phosphate, Eosin Y, methylene blue, agar etc.Belong to weak selective medium, for separated and the evaluation, particularly intestinal bacteria of Gram-negative intestinal bacilli.SS nutrient agar main component has beef extract powder, peptone, no. 3 bile salt, lactose, Trisodium Citrate, ironic citrate, Sulfothiorine, toluylene red, brilliant green, agar etc.Be used for the separated of Salmonellas and some Shigellae.According to the specificity of adding in substratum, differentiate material, can observe preliminary judgement pathogenic bacterium kind by surfaces such as colonial morphology, colors.
During use, by sample inoculation line in the eosin methylene blue agar //SS agar lattice of same flat board simultaneously, put in constant temperature incubator and cultivate, observations.If bacillus coli is in the methylene blue plate culture medium of Yihong, be atropurpureus bacterium colony, there is green metal gloss, in SS Agar Plating, be pink bacterium colony, or have cholate precipitation.Salmonella typhimurium is colourless and in SS Agar Plating, is colourless bacterium colony to amber bacterium colony in the methylene blue plate culture medium of Yihong, has evil mind.Therefore,, by the cultivation of lattice plate culture medium is observed, can tentatively determine the concrete pathogen enterobacteria kind that whether contains pathogen enterobacteria and contain in sample.
As shown in Figure 2, a kind of vertically dividing equally check into three plate culture medium, comprises the aseptic plate of vertically dividing equally check into three, Colombia nutrient agar matrix C, china blue agar medium matrix D, chocolate medium matrix E through the ages.
Colombia's blood agar culture-medium main component has peptone, sodium-chlor, beef heart infusion, Zulkovsky starch, agar etc., for the cultivation to the overcritical bacterium of nutrition.China blue agar substratum main component has beef extract powder, peptone, lactose, sodium-chlor, lokav, agar etc., for the separation and Culture of pathogen enterobacteria, and the particularly separation of salmonella and Shigella bacterium.Chocolate substratum is to add vancomycin in ordinary chocolate look nutrient agar through the ages, for the separation and Culture of Neisseria and influenzae.
During use, by simultaneously inoculation line respectively in the chocolate culture-medium lattice of Colombia's agar/china blue agar of same flat board/through the ages of sample, put in constant temperature incubator and cultivate, observations.According to colony colour, growing state, hemolytic reaction etc., judge the bacterial classification kind in sample.
Embodiment 4
As shown in Figure 2, a kind of vertically dividing equally check into three plate culture medium, comprises the aseptic plate of vertically dividing equally check into three, Colombia nutrient agar matrix C, maconkey agar medium matrix D, chocolate medium matrix E.
Colombia's blood agar culture-medium main component has peptone, sodium-chlor, beef heart infusion, Zulkovsky starch, agar etc., for the cultivation to the overcritical bacterium of nutrition.Maconkey agar substratum main component has peptone, lactose, sodium-chlor, cholate, toluylene red, agar etc., for separating of with differentiate intestinal bacteria.Chocolate substratum is for the separation and Culture of Neisseria and influenzae.
During use, by sample inoculation line respectively in Colombia's agar/maconkey agar/chocolate substratum lattice of same flat board simultaneously, put in constant temperature incubator and cultivate, observations.According to colony colour, growing state, hemolytic reaction etc., judge the bacterial classification kind in sample.
Above-described embodiment is not limitation of the utility model; the utility model is also not limited to above-mentioned giving an example, and those skilled in the art, in essential scope of the present utility model; the variation of making, interpolation or replacement, all should belong to protection domain of the present utility model.
Claims (4)
1. a lattice plate culture medium, comprise dull and stereotyped culture dish (1), it is characterized in that: in described culture dish (1), be provided with several dividing plates (2) of being divided equally, culture dish (1) is divided into several minute space (3) by dividing plate (2) minute different substratum of the interior splendid attire in space (3).
2. the dull and stereotyped culture dish of lattice according to claim 1, is characterized in that: described culture dish (1) is the transparent culture dish of aseptic plastic.
3. lattice plate culture medium according to claim 1, is characterized in that: described dividing plate (2) is one, and culture dish (1) is divided into two minutes spaces (3).
4. lattice plate culture medium according to claim 1, is characterized in that: described dividing plate (2) is three, and culture dish (1) is divided into three minutes spaces (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320466479.9U CN203451536U (en) | 2013-08-01 | 2013-08-01 | Mesh division plate culture medium |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320466479.9U CN203451536U (en) | 2013-08-01 | 2013-08-01 | Mesh division plate culture medium |
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| CN203451536U true CN203451536U (en) | 2014-02-26 |
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| CN201320466479.9U Expired - Fee Related CN203451536U (en) | 2013-08-01 | 2013-08-01 | Mesh division plate culture medium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103397153A (en) * | 2013-08-06 | 2013-11-20 | 洛阳市西格马仪器制造有限公司 | Isothermal air circulation heat-treatment electric furnace |
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2013
- 2013-08-01 CN CN201320466479.9U patent/CN203451536U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103397153A (en) * | 2013-08-06 | 2013-11-20 | 洛阳市西格马仪器制造有限公司 | Isothermal air circulation heat-treatment electric furnace |
| CN103397153B (en) * | 2013-08-06 | 2015-07-08 | 洛阳市西格马仪器制造有限公司 | Isothermal air circulation heat-treatment electric furnace |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP02 | Change in the address of a patent holder |
Address after: 250101 workshop 303, building 5, industrialization base of small and medium sized enterprises in biological medicine Park, 1777 Dazheng Road, high tech Zone, Jinan City, Shandong Province Patentee after: JINAN BAIBO BIOTECHNOLOGY Co.,Ltd. Address before: 250101 C, 802, century wealth center, east of Chong Hua Road, Ji'nan hi tech Zone, Shandong, China Patentee before: JINAN BAIBO BIOTECHNOLOGY Co.,Ltd. |
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| CP02 | Change in the address of a patent holder | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140226 Termination date: 20210801 |
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| CF01 | Termination of patent right due to non-payment of annual fee |