CN203432833U - Antiacid staining liquid quality control substance - Google Patents
Antiacid staining liquid quality control substance Download PDFInfo
- Publication number
- CN203432833U CN203432833U CN201320554977.9U CN201320554977U CN203432833U CN 203432833 U CN203432833 U CN 203432833U CN 201320554977 U CN201320554977 U CN 201320554977U CN 203432833 U CN203432833 U CN 203432833U
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- China
- Prior art keywords
- acid
- liquid quality
- fast stain
- antiacid
- control product
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- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model relates to the technical field of bio-medical treatment and provides an antiacid staining liquid quality control substance. The antiacid staining liquid quality control substance comprises a glass slide and a coating layer, wherein mycobacterium tuberculosis H37Ra and one or more than two antiacid staining negative strains are attached to the surface of the coating layer; the glass slide is a smooth glass slice or a glass slice of which one side is ground and the other side is smooth; the antiacid staining negative strains are coccuses or bacilluses or mixtures of the coccuses and the bacilluses. The antiacid staining liquid quality control substance has the characteristics of saving time, improving the working efficiency of a worker, detecting the quality of staining liquid and supervising the worker along with simplicity in operation.
Description
Technical field
The utility model belongs to bio-medical technology field, particularly a kind of acid-fast stain liquid quality-control product.
Background technology
Ziehi-Neelsen stain is the bacterial stain of being initiated by Paul Ehrlich for 1882 and createing through Qi Er improvement.The cell membrane of mycobacterium contains a large amount of lipids, is enclosed in the outside of peptide glycan, so the general easy coloring not of mycobacterium will and extend dyeing time through heating and impel it painted.But after the mycolic acid in mycobacterium is combined with dyestuff, be just difficult to by acid bleaching agent bleaching therefore named acid-fast stain.Together-Nissl Ziehi-Neelsen stain is under heating condition, to make mycolic acid become compound with carbolfuchsin strong bonded, with hydrochloride alcohol, processes also nondiscoloration.After adding alkaline methylene blue again and redying, mycobacterium is still red, and material in other bacteriums and background be blueness.
Ziehi-Neelsen stain generally comprises: just dye, decolour, redye three steps, concrete operation method is: the making of nacterial smear sample and fixing; With glass slide clamp, clamp smear preparation, drip carbolfuchsin 2-3 and drip, in the heating slowly of flame eminence, be sure not boiling, occur that steam is away from keyboard, if dye liquor evaporation reduces, should add again dye liquor, so as not to dry, heating 3-5 minute, after sample is cooling, water rinses; Add 3% hydrochloride alcohol decolouring 30 seconds ~ 1 minute, water rinses; With alkaline methylene blue solution, redye 1 minute, washing, blots the rear oily sem observation of using with thieving paper.
Acid-fast stain three-step approach effect is fine, but operation more complicated, the every operation of staff once will be done nacterial smear sample one time, lose time, and inefficiency, dyeing liquor quality, dyeing time length, staff's operative technique skill level are had to very high requirement, but existing market is without any a ready made nacterial smear sample, while working for staff, directly brings.
Therefore, bio-medical technology field be badly in need of a kind ofly saving time, simple to operate, improve staff's work efficiency, can detect dyeing liquor quality, staff is played to the acid-fast stain liquid quality-control product of supervisory role.
Utility model content
A kind of acid-fast stain liquid quality-control product of the present utility model, technical scheme is as follows:
A kind of acid-fast stain liquid quality-control product of the present utility model, is characterized in that, comprises:
Microslide, surface has H37Ra tubercle bacillus and acid-fast stain negative strain coating.
As above a kind of acid-fast stain liquid quality-control product, wherein, microslide is smooth glass sheet.
As above a kind of acid-fast stain liquid quality-control product, wherein, on one side microslide has frosted, another side is smooth glass sheet.
As above a kind of acid-fast stain liquid quality-control product, wherein, acid-fast stain negative bacterium is one kind or two or more bacterial strain.
As above a kind of acid-fast stain liquid quality-control product, wherein, acid-fast stain negative bacterium is coccus.
As above a kind of acid-fast stain liquid quality-control product, wherein, acid-fast stain negative bacterium is bacillus.
As above a kind of acid-fast stain liquid quality-control product, wherein, acid-fast stain negative bacterium is the potpourri of coccus and bacillus.
The invention has the beneficial effects as follows:
By the acid-fast stain liquid quality-control product preparing, carry out the process of just dying, decolour, redying with the new smear of making simultaneously, whether expiredly can detect dyeing liquor.
By the acid-fast stain liquid quality-control product preparing, carry out the process of just dying, decolour, redying with the new smear of making simultaneously, can operating personnel's operation steps, skill level be exercised supervision and be checked.
The present invention is simple to operate, save time.
The present invention has improved operating personnel's work efficiency, thereby has improved economic benefit, has applicability more widely.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, describe the utility model in detail:
Fig. 1 is the structural representation of a kind of acid-fast stain liquid quality-control product of the present utility model.
Embodiment
In order to make technological means of the present utility model, creation characteristic, reach object and effect is easy to understand, below in conjunction with concrete diagram, further set forth the utility model.
Fig. 1 is the structural representation of a kind of acid-fast stain liquid quality-control product of the present utility model.
A kind of acid-fast stain liquid quality-control product of the present utility model, is characterized in that, comprises: microslide 1, surface has H37Ra tubercle bacillus and acid-fast stain negative strain coating 2.
Microslide 1 surface is for smooth glass sheet or have frosted on one side, and another side is smooth glass sheet.
Acid-fast stain negative bacterium is a kind or 2 kinds or multiple bacterial strain.
Acid-fast stain negative bacterium is the potpourri of coccus or bacillus, coccus and bacillus.
When using the utility model to do acid-fast stain, first the microslide 1 use glass slide clamp that scribbles H37Ra tubercle bacillus and acid-fast stain negative strain is clamped to smear preparation, drip carbolfuchsin 2-3 and drip, in the heating slowly of flame eminence, be sure not boiling, occur that steam is away from keyboard, if dye liquor evaporation reduces, should add again dye liquor, in order to avoid dry, heating 3-5 minute, after sample is cooling, water rinses; Add 3% hydrochloride alcohol decolouring 30 seconds ~ 1 minute, water rinses; With alkaline methylene blue solution, redye 1 minute, washing, blots the rear oily sem observation of using with thieving paper, and H37Ra tubercle bacillus presents redness and acid-fast stain negative strain presents blueness.
Get smear and the acid-fast stain liquid quality-control product of the present utility model that staff newly makes and carry out mark, repeat above-mentioned experiment, whether well according to the color of H37Ra tubercle bacillus and acid-fast stain negative strain, can judge dyeing liquor, whether bacterial classification is good, and supervision work personnel's careful degree and skill level.
The invention has the beneficial effects as follows:
1. by the acid-fast stain liquid quality-control product preparing, carry out the process of just dying, decolour, redying with the new smear of making simultaneously, whether expiredly can detect dyeing liquor.
2. by the acid-fast stain liquid quality-control product preparing, carry out the process of just dying, decolour, redying with the new smear of making simultaneously, can operating personnel's operation steps, skill level be exercised supervision and be checked.
The present invention simple to operate, save time.
4. the present invention has improved operating personnel's work efficiency, thereby has improved economic benefit, has applicability more widely.
More than show and described ultimate principle of the present utility model, principal character and advantage of the present utility model.The technician of the industry should understand; the utility model is not restricted to the described embodiments; that in above-described embodiment and instructions, describes just illustrates principle of the present utility model; under the prerequisite that does not depart from the utility model spirit and scope, the utility model also has various changes and modifications, and these changes and improvements all fall within the scope of claimed the utility model.The claimed scope of the utility model is defined by appending claims and equivalent thereof.
Claims (6)
1. an acid-fast stain liquid quality-control product, is characterized in that, comprises:
Microslide, surface has H37Ra tubercle bacillus and acid-fast stain negative strain coating.
2. a kind of acid-fast stain liquid quality-control product as claimed in claim 1, is characterized in that, this microslide is smooth glass sheet.
3. a kind of acid-fast stain liquid quality-control product as claimed in claim 1, is characterized in that, on one side this microslide has frosted, another side is smooth glass sheet.
4. a kind of acid-fast stain liquid quality-control product as claimed in claim 1, is characterized in that, this acid-fast stain negative strain is a kind or 2 kinds or multiple bacterial strain.
5. a kind of acid-fast stain liquid quality-control product as claimed in claim 1, is characterized in that, this acid-fast stain negative strain is coccus.
6. a kind of acid-fast stain liquid quality-control product as claimed in claim 1, is characterized in that, this acid-fast stain negative strain is bacillus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320554977.9U CN203432833U (en) | 2013-09-09 | 2013-09-09 | Antiacid staining liquid quality control substance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320554977.9U CN203432833U (en) | 2013-09-09 | 2013-09-09 | Antiacid staining liquid quality control substance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203432833U true CN203432833U (en) | 2014-02-12 |
Family
ID=50061864
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320554977.9U Expired - Lifetime CN203432833U (en) | 2013-09-09 | 2013-09-09 | Antiacid staining liquid quality control substance |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203432833U (en) |
-
2013
- 2013-09-09 CN CN201320554977.9U patent/CN203432833U/en not_active Expired - Lifetime
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 200335 No. 74 clear pool road, Shanghai, Changning District Patentee after: SHANGHAI LABWAY CLINICAL LABORATORY Co.,Ltd. Address before: 200335 No. 74 clear pool road, Shanghai, Changning District Patentee before: Shanghai Lan Wei medical laboratory Co.,Ltd. Address after: 200335 No. 74 clear pool road, Shanghai, Changning District Patentee after: Shanghai Lan Wei medical laboratory Co.,Ltd. Address before: 200335 No. 74 clear pool road, Shanghai, Changning District Patentee before: Shanghai Labway Clinical Examination Co.,Ltd. |
|
| CX01 | Expiry of patent term |
Granted publication date: 20140212 |
|
| CX01 | Expiry of patent term |