CN203414467U - Up-converting phosphor immunochromatographic test paper for quantitative determination of methylamphetamine concentration in urine - Google Patents
Up-converting phosphor immunochromatographic test paper for quantitative determination of methylamphetamine concentration in urine Download PDFInfo
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Abstract
The utility model relates to up-converting phosphor immunochromatographic test paper for quantitative determination of methylamphetamine concentration in urine. The test paper comprises a stripped sample pad [1], a combining pad [2], an analyzing film [3], a water absorption pad [4], a sticky bottom liner [5] and a Max line indicating paste [6] which are equal in width, wherein the water absorption pad and the analyzing film form a first combining area, the combining pad and the analyzing film form a second combining area, the sample pad and the combining pad form a third combining area, and the Max line indicating paste is adhered to the combining pad and the sample pad. One side of the Max line indicating paste is flush with the edge of the combining pad while the other side of the Max line indicating paste is slightly shorter than the sample pad. Length and the slight short length of each combining area are 3/10-2/5 of that of the corresponding pad. The test paper provided by the utility model has the characteristics of fastness and stability, accurate size and high pertinence, and is particularly applicable to quick quantitative determination of methylamphetamine in urine on the scene.
Description
Invention field
The utility model relates to a kind of test paper structure based on upper forwarding light immunochromatography technique, particularly a kind ofly can carry out to crystal methamphetamine concentration in urine the test paper based on upper forwarding light immunochromatography technique of accurate quantification detection.
Background technology
Crystal methamphetamine (methamphetamine, MA) is a kind of amphetamine material with stimulating central nervous system effect, because of the pure white sparkling and crystal-clear seemingly ice of its outward appearance, and is commonly called as ice.Methamphetamine has strong central nervous excitation effect, because its pungency is strong and lasting, using once just can be addicted, toxicity is better than the king's of Poison the heroin of title, therefore by prophesy, will progressively be replaced popular opium in last century, heroin, hemp, cocaine etc., become 21 century abuse drugs the most widely.Crystal methamphetamine is one of China's most important drugs of monitoring at present, banning.
The detection method of crystal methamphetamine has almost comprised the every field of Modern Analytical Chemistry, has chromatography, spectroscopic methodology, mass spectroscopy etc., also relates to the multiple classical technology of Biological Detection, as immunochromatographic method, enzyme linked immunosorbent assay etc.Restriction due to technical merit, the detection of p-Methylamphetamine mainly adopts the method for confirming after first examination in the world at present, first adopt Quick qualitative detection method to carry out examination to drug abuse colony, and then by accurate quantification method, the sample of the primary dcreening operation positive is confirmed.
The prescreening method of main flow is colloidal gold immunity chromatography, and it is a kind ofly based on the siphon of multiple poromerics side direction capillary, to reach particular target, to catch the solid phase labelling immunoassay with spike.The advantages such as it is easy, quick and cheap that the method has, but have that susceptibility is poor, a subjective sentence read result of naked eyes, the technological deficiency that cannot accurate quantification etc. cannot overcome.The technology that other relates in primary dcreening operation also comprises radio immunoassay and enzyme linked immunosorbent assay (ELISA).The amount of samples of radio immunoassay is few and without pre-service, accuracy is higher, but operating personnel and environment are had to larger harm; ELISA cost is lower, and speed is highly sensitive, but sample needs certain pre-service, reaches 2-3 hour detection time, needs microplate reader, and operating personnel's professional standards are had relatively high expectations, and is difficult to realize on-the-spot on-line monitoring and the detection of real meaning.
Confirmation method mainly contains, high performance liquid chromatography (HPLC) is gentle/matter combination analysis method (GC-MS).HPLC and GC-MS method have accurate, reproducible, are applicable to the advantages such as quantitatively detection, are the quantivative approachs of current most widely used general, the most authoritative confirmation drugs.But these two kinds of methods all need sample to carry out pre-service, complex operation, has higher requirement to operating personnel's specialized capability and the level of being skilled in technique; And the instrument of these two kinds of methods is more expensive, cost is higher, therefore can only orientate as " primary dcreening operation complete after for positive, by professional and technical personnel, in laboratory, carried out result and check ", be not suitable for on-the-spot primary dcreening operation fast.
The technological deficiency that the immunochromatography technique that relies on for crystal methamphetamine primary dcreening operation exists, the appearing as it breakthrough point solving be provided of a kind of novel upper forwarding luminescent material (Up-Converting Phosphor, UCP).UCP is novel rare earth metal crystal optics material, the physical arrangement that it is unique and chemical composition make it have the non-existent upper forwarding optical phenomenon of occurring in nature, be that UCP particle can be by infrared ray excited, transmitting visible ray, thus for all UCP of usining particles have established as the detection technique of biological tracer the solid foundation that high s/n ratio, precise quantification are analyzed; In dependence, turn luminescence technology (Up-converting Phosphor Technology, UPT) facilitated immunochromatography technique and UCP to be combined into forwarding light immunochromatography technique (Up-converting Phosphor Technology based Lateral Flow assay, UPT-LF assay), by after a series of finishinges and activation, UCP particle being applied to immunochromatography as tracer, the upper forwarding optical phenomenon with uniqueness under Infrared irradiation discloses high responsive special identification between bioactive molecule.In use, forwarding light immunity analysis instrument can excite, receive, change the specific detection signal being produced by UCP on UPT immune chromatography test paper, thus the accurate quantitative analysis of realize target checking matter.In UPT-LF detection paper process, sample is without pre-service, within after application of sample several minutes, can complete detection, commercially available upper forwarding light immunity analysis instrument is cheap, easy and simple to handle, be easy to carry about with one, can obtain quantitative result in several seconds interscan, therefore without specialty background personnel, also can realize fast at the scene the accurate quantification detection of particular target.As can be seen here, the optical characteristics by UCP uniqueness has solved that traditional immunochromatography technique susceptibility is poor, problem that can not accurate quantification targetedly, has retained the simple and efficient technical advantage of chromatographic technique simultaneously.
In sum, utilize UPT-LF to carry out accurate quantification detection to crystal methamphetamine concentration in urine, will significantly promote accuracy, the reliability of the on-the-spot primary dcreening operation of crystal methamphetamine.
Yet, current each regional structure of existing immune chromatography test paper is relatively loose, have the shortcoming that is easy to be scattered in test paper loading, and test paper structure p-Methylamphetamine characteristic specific aim is poor, is therefore necessary the upper forwarding light immune chromatography test paper that design quantitatively detects for crystal methamphetamine.
Invention technology
In order to overcome the defect existing in prior art, the utility model object is to provide a kind of structure more firmly crystal methamphetamine concentration in urine to be carried out to upper forwarding light immunochromatography (UPT-LF) test paper quantitatively detecting.
A kind of upper forwarding light immunochromatography (UPT-LF) test paper that crystal methamphetamine concentration in urine is carried out to quantitative detection that the utility model provides, comprise: wide ribbon sample pad, pad, analyzing film, adsorptive pads, viscosity end liner and Max line indicating sticker, wherein, sample pad, pad, analyzing film and adsorptive pads are pasted on viscosity end liner successively, wherein analyzing film is affixed on the centre of viscosity end liner, adsorptive pads is affixed on a side of analyzing film and forms the first land with analyzing film, pad is affixed on the opposite side of analyzing film and forms the second land with analyzing film, sample pad is affixed on the opposite side of pad and with pad and forms the 3rd land, Max line indicating sticker is pasted in pad and sample pad and covers pad, described the first land length is the 3/10-2/5 of adsorptive pads length, and described the second land length is the 3/10-2/5 of pad length, described the 3rd land length is the 3/10-2/5 of sample pad length.
Wherein, Max line indicating sticker is concordant with pad with the edge of the overlapping side of pad, slightly shorter than print pad with the edge of the overlapping side of sample pad, and slightly short partial-length is the 3/10-2/5 of sample pad length.
Concrete, described the first land length is 7/20 of adsorptive pads length; Described the second land length is 7/20 of pad length; Described the 3rd land length is 7/20 of sample pad length;
On analyzing film, in length direction spacing, be provided with parallel detection band T and quality control band C, detect in band T, be coated with methyl Ben Bing An ?the crosslinked antigen of bovine serum albumin(BSA), in quality control band C, be coated with the anti-sheep IgG of rabbit.
In pad, be fixed with UCP bond potpourri, UCP bond potpourri comprises: detect bond and Quality Control bond, detect bond and be UCP ?crystal methamphetamine monoclonal antibody bond, Quality Control bond be UCP ?sheep IgG bond;
Sample pad is the urea removal pad of processing through damping fluid, surfactant, closed protein, inorganic salts.
Adopt above scheme, the utility model carries out upper forwarding light immunochromatography (UPT-LF) test paper quantitatively detecting to crystal methamphetamine concentration in urine, the overlapping region that whole test paper relates to comprises " the first land that adsorptive pads and analyzing film are overlapping ", " the second land that pad and analyzing film are overlapping ", " the 3rd land that sample pad and pad are overlapping ", all adopt the partially slight overlap mode of moderate, according to the combination of determined detection object crystal methamphetamine and reagent and the features of movement on test paper thereof, design overlap margin for 3/10-2/5(be separately 6/20-8/20), thereby take into account liquid sample mixes in adequacy and the abundant infiltration of liquid sample in sample pad and pad of the inner lateral flow of test paper each several part simultaneously, guarantee on the one hand comparatively suitable flowing time, make on the other hand the detection target in sample fully react with large overlapping being able to of pad by sample pad with detection bond, " Max line indicating sticker is pasted pad, sample pad and covering pad " overlap mode, on the one hand, the indicating sticker all standing of Max line and stickup, be equivalent to a upper cover, can make to be clipped in its pad between viscosity end liner is combined more firm with sample pad, guarantee the stability of overlapping region, avoid occurring being scattered in test paper loading, on the other hand, Max line indicating sticker is made with water proof material, Max line indicating sticker, pad, sample pad, viscosity end liner stacked and fixing successively, the Max line indicating sticker having formed at water proof and the flow channel for liquids between viscosity end liner, avoid the direct transregional flow of liquid sample.
The utility model is by combining with immunochromatography technique UCP particle as tracer, utilize UCP tracer without the high s/n ratio optical signalling of background interference, significantly promoted the detection sensitivity of traditional chromatographic technique, and the ability of giving its accurate quantification, simultaneously completely retained that immunochromatography technique is easy and simple to handle, advantage efficiently.Thereby, final upper forwarding light immunochromatography (UPT-LF) test paper that crystal methamphetamine concentration in urine is carried out to quantitative detection of setting up, integrated easy and simple to handle, quick, susceptibility is high, specificity good, can accurate quantification etc. many characteristics, especially suitablely at the scene the crystal methamphetamine in urine is carried out to Quantitative detection.Will significantly promote accuracy, the reliability of the on-the-spot primary dcreening operation of crystal methamphetamine.
Accompanying drawing explanation:
Fig. 1: the utility model crystal methamphetamine quantitative testing test paper three-dimensional structure diagram;
Fig. 2: the utility model crystal methamphetamine quantitative testing test paper cross-sectional view;
Fig. 3: the utility model crystal methamphetamine quantitative testing test paper is pasted schematic diagram;
Fig. 4: the typical curve that crystal methamphetamine standard items concentration in urine is detected.
1, sample pad, 2, pad, 3, analyzing film, 4, adsorptive pads, 5, viscosity end liner, 6, Max line indicating sticker, 7, UCP bond potpourri, 8, UCP-crystal methamphetamine monoclonal antibody bond, 9, UCP-sheep IgG bond, 10, detection band T, 11, quality control band C.
Below in conjunction with accompanying drawing, further illustrate the utility model.
Embodiment:
Shown in Fig. 1 and Fig. 2, the utility model provides crystal methamphetamine concentration in urine is carried out to upper forwarding light immunochromatography (UPT-LF) test paper quantitatively detecting, and wide ribbon sample pad [1], pad [2], analyzing film [3], adsorptive pads [4], viscosity end liner [5], several parts of Max line indicating sticker [6], consists of.Wherein:
Sample pad [1] is the urea removal pad of processing through damping fluid, surfactant, closed protein, inorganic salts;
In pad [2], be fixed with UCP bond potpourri [7], UCP bond potpourri [7] comprising: detect bond and Quality Control bond, detecting bond is UCP-crystal methamphetamine monoclonal antibody bond [8], and Quality Control bond is UCP-sheep IgG bond [9];
On analyzing film [3], be provided with and detect band T[10], quality control band C[11], two bands are parallel to each other in the longitudinal direction and have certain intervals; Detect band T[10] be coated with the crosslinked antigen of crystal methamphetamine-bovine serum albumin(BSA), quality control band C[11] be coated with the anti-sheep IgG of rabbit;
Sample pad [1], pad [2], analyzing film [3] and adsorptive pads [4] are pasted on viscosity end liner [5] successively, wherein analyzing film [3] is affixed on the centre of viscosity end liner [5], adsorptive pads [4] is affixed on a side of analyzing film [3], both intersection form the first land, the first land length L 1 is the 3/10-2/5 of adsorptive pads [4] length, preferably 7/20; Pad [2] is affixed on the opposite side of analyzing film [3], and both intersection form the second land, and the second land length L 2 is the 3/10-2/5 of pad [2] length, and preferably 7/20; Sample pad [1] is affixed on the opposite side of pad [2], and both intersection form the 3rd lands, and the 3rd land length L 3 is the 3/10-2/5 of sample pad [1] length, and preferably 7/20; Max line indicating sticker [6] is pasted on pad [2] and sample pad [1] and covers pad [2], and wherein, with the overlapping side of pad [2], edge is concordant with pad [2]; With the overlapping side of sample pad [1], edge is slightly shorter than print pad [1], and slightly short partial-length L4 is the 3/10-2/5 of sample pad [1] length, and preferably 7/20.
On this, the preparation method of forwarding light immunochromatography (UPT-LF) test paper is:
A. the preparation of sample pad [1]: 4ml sample pad treating fluid (pH7.2 phosphate buffer, containing 0.01%SDS, 0.5% skimmed milk power, 2%NaCl) is added drop-wise on the urea removal pad of 20cm * 1.5cm, at 37 ℃, dries 2h, obtain sample pad [1], standby;
B. the preparation of pad [2]: will detect bond (UCP-crystal methamphetamine monoclonal antibody bond [8]) and Quality Control bond (UCP-sheep IgG bond [9]) and mix, obtain UCP bond potpourri [7], with bond dilution (pH7.2 phosphate buffer, containing 1% skimmed milk power) UCP bond potpourri [7] is diluted to final concentration is 2mg/ml, UCP bond potpourri [7] is added on glass fibre, polyester film or nonwoven fabrics, at 45 ℃, dry 1.5h, obtain pad [2], standby;
C. the preparation of analyzing film [3]: the crosslinked antigen of 2mg/ml crystal methamphetamine-bovine serum albumin(BSA) and the anti-sheep IgG of 2mg/ml rabbit specking are with to T[10 as detecting respectively on nitrocellulose filter or nylon membrane] and quality control band C[11], at 37 ℃, dry 1h, obtain analyzing film [3], standby;
D. sample pad [1], pad [2], analyzing film [3] and adsorptive pads [4] are pasted on viscosity end liner [5] (can select the water proof materials such as PVC plate) successively, Max line indicating sticker [6] (can select the water proof materials such as PVC film) is pasted on to sample pad [1], pad [2] above, guarantees overlapping relation (as shown in Figure 3) each other; Test paper is cut into the wide available finished product separately of 4mm, obtains crystal methamphetamine concentration quantitative Test paper in the utility model urine; The test paper of moulding can directly be used or insert in plastic casing and use.
Upper forwarding light immunochromatography (UPT-LF) test paper that uses the utility model to carry out quantitatively detecting to crystal methamphetamine concentration in urine quantitatively detects the method for crystal methamphetamine:
A. add sample: testing sample (urine) 100 μ l/ bars are dropped in the sample pad [1] of above-mentioned the utility model crystal methamphetamine quantitative testing test paper;
B. chromatography reaction: treat that chromatography has reacted for standing several minutes;
C. result interpretation: with commercially available upper forwarding light immunity analysis instrument, test paper is scanned, will detect band T[10] and quality control band C[11] signal intensity (being peak area) successively assignment in T and C, the detected value that T/C is crystal methamphetamine;
D. result is quantitative: p-Methylamphetamine series concentration standard items are measured and obtained the T/C value corresponding with each concentration successively, using T/C value as X, using standard items concentration as Y, drawing standard concentration working curve fitting quantitative computing formula, the quantitative computing formula of T/C value substitution that each sample detection is obtained can obtain the wherein accurate concentration of contained crystal methamphetamine.
Crystal methamphetamine concentration in urine is carried out to upper forwarding light immunochromatography (UPT-LF) the detection paper crystal methamphetamine standard items susceptibility and the range of linearity that quantitatively detect:
By the crystal methamphetamine standard items purchased from Material Evidence Identification Center, Ministry of Public Security, with after standard urinary dilution, 100 μ l/ bars are added on the utility model crystal methamphetamine quantitative testing test paper, scanning after chromatography number minute, and typical curve is shown in Fig. 4, the experimental result drawing is as table 1:
Table 1: the utility model crystal methamphetamine quantitative testing test paper detects crystal methamphetamine standard items susceptibility
Conclusion: the susceptibility of this crystal methamphetamine quantitative testing test paper is 100ng/ml, can accurate quantification in the scope of 100-5000ng/ml.Its quantitative computing formula is as listed in Fig. 4.
Claims (7)
1. one kind is carried out the quantitatively upper forwarding light immune chromatography test paper of detection to crystal methamphetamine concentration in urine, it is characterized in that, the composition structure of this test paper comprises: wide ribbon sample pad [1], pad [2], analyzing film [3], adsorptive pads [4], viscosity end liner [5], Max line indicating sticker [6], wherein, sample pad [1], pad [2], analyzing film [3] and adsorptive pads [4] are pasted on viscosity end liner [5] successively, wherein analyzing film [3] is affixed on the centre of viscosity end liner [5], adsorptive pads [4] is affixed on a side of analyzing film [3] and forms the first land with analyzing film [3], pad [2] is affixed on the opposite side of analyzing film [3] and forms the second land with analyzing film [3], sample pad [1] is affixed on the opposite side of pad [2] and forms the 3rd land with pad [2], Max line indicating sticker [6] is pasted on pad [2] and sample pad [1] is upper and covering pad [2], described the first land length is the 3/10-2/5 of adsorptive pads [4] length, and described the second land length is the 3/10-2/5 of pad [2] length, described the 3rd land length is the 3/10-2/5 of sample pad [1] length.
2. upper forwarding light immune chromatography test paper as claimed in claim 1, it is characterized in that, Max line indicating sticker [6] is concordant with pad [2] with the edge of the overlapping side of pad [2], slightly shorter than print pad [1] with the edge of the overlapping side of sample pad [1], slightly short partial-length is the 3/10-2/5 of sample pad [1] length.
3. upper forwarding light immune chromatography test paper as claimed in claim 1 or 2, is characterized in that, described the first land length is 7/20 of adsorptive pads [4] length; Described the second land length is 7/20 of pad [2] length; Described the 3rd land length is 7/20 of sample pad [1] length.
4. upper forwarding light immune chromatography test paper as claimed in claim 3, is characterized in that, described Max line indicating sticker [6] is made by water proof material.
5. upper forwarding light immune chromatography test paper as claimed in claim 4, it is characterized in that, analyzing film [3] is upper is provided with parallel detection band T[10 in length direction spacing] and quality control band C[11], detect band T[10] in be coated with the crosslinked antigen of crystal methamphetamine-bovine serum albumin(BSA), quality control band C[11] in be coated with the anti-sheep IgG of rabbit.
6. upper forwarding light immune chromatography test paper as claimed in claim 5, it is characterized in that, in pad [2], be fixed with UCP bond potpourri [7], UCP bond potpourri [7] comprising: detect bond and Quality Control bond, detecting bond is UCP-crystal methamphetamine monoclonal antibody bond [8], and Quality Control bond is UCP-sheep IgG bond [9];
7. upper forwarding light immune chromatography test paper as claimed in claim 6, is characterized in that, sample pad [1] is the urea removal pad of processing through damping fluid, surfactant, closed protein, inorganic salts.
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| CN201320550667.XU CN203414467U (en) | 2013-09-05 | 2013-09-05 | Up-converting phosphor immunochromatographic test paper for quantitative determination of methylamphetamine concentration in urine |
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| CN201320550667.XU CN203414467U (en) | 2013-09-05 | 2013-09-05 | Up-converting phosphor immunochromatographic test paper for quantitative determination of methylamphetamine concentration in urine |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110726835A (en) * | 2014-12-29 | 2020-01-24 | 张红 | Immune lateral chromatography detection method and device for externally driving biomarker |
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2013
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110726835A (en) * | 2014-12-29 | 2020-01-24 | 张红 | Immune lateral chromatography detection method and device for externally driving biomarker |
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Address after: 100071 Fengtai District East Street, No. 20, Beijing Patentee after: INSTITUTE OF MICROBIOLOGY AND EPIDEMIOLOGY, ACADEMY OF MILITARY MEDICAL SCIENCES, PR CHINA Patentee after: China Intellectech (Beijing) Corp. Patentee after: BEIJING HOTGEN BIOTECH Co.,Ltd. Address before: 100071 Fengtai District East Street, No. 20, Beijing Patentee before: Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, PR China Patentee before: China Intellectech (Beijing) Corp. Patentee before: BEIJING HOTGEN BIOTECH Co.,Ltd. |
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| CX01 | Expiry of patent term |
Granted publication date: 20140129 |
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