CN203385735U - Kit for conveniently sucking sample and detecting IgM - Google Patents
Kit for conveniently sucking sample and detecting IgM Download PDFInfo
- Publication number
- CN203385735U CN203385735U CN201320626797.7U CN201320626797U CN203385735U CN 203385735 U CN203385735 U CN 203385735U CN 201320626797 U CN201320626797 U CN 201320626797U CN 203385735 U CN203385735 U CN 203385735U
- Authority
- CN
- China
- Prior art keywords
- sample
- pad
- tube body
- igg
- reaction film
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model discloses a kit for conveniently sucking a sample and detecting IgM. The kit comprises a pasteur pipet and a cap matched with the pasteur pipet, wherein a colloidal gold test paper strip is arranged in the pasteur pipet and comprises a sample pad, a gold-labeled pad, a reaction film, a water absorption pad and an IgG filter film enveloped with an anti-human IgG antibody; a detection line enveloped with an antigen and a quality control line enveloped with anti-rat IgG are arranged on the reaction film; one end of the gold-labeled pad is overlapped on the upper surface of one end of the reaction film; the IgG filter film is overlapped on the upper surface of the other end of the gold-labeled pad; the upper surface of one end, which is away from the gold-labeled pad, of the IgG filter film is overlapped with the sample pad; the upper surface of the other end of the reaction film is overlapped with the water absorption pad; two ends of the upper surfaces of the water absorption pad and the sample pad are adhered to the inner wall of a sharp glass pipe; the sample pad of the colloidal gold test paper strip is close to a sharp of the sharp glass pipe. The kit can suck the sample conveniently and is difficult to cause pollution; the influence of IgG on IgM can be eliminated; the accuracy of IgM antibody detection is improved.
Description
Technical field
The utility model relates to the immunology detection device, particularly a kind of convenient kit of drawing the detection IgM of sample.
Background technology
At present, the kit that IgM detects comprises the test strips be arranged in box body, test strips comprises backer board, reaction film, is coated with gold mark pad, sample pad, the adsorptive pads of the anti-human IgM monoclonal antibody of colloid gold label, described reaction film sticks to the backer board upper surface, described reaction film is provided with the detection line of envelope antigen and the nature controlling line of coated anti-mouse IgG, one end of described gold mark pad rides over the upper surface of reaction film one end, described sample pad rides over the upper surface of the other end of described gold mark pad, and an end of adsorptive pads rides over the upper surface of the other end of reaction film.The test strips of this spline structure, the pathogen specific IgG antibody of high-titer can cause false negative with IgM antibody competition antigen binding site; Because the IgM class RF existed in serum can be combined with IgG antibody, then be attached on the antigen detection line, easily cause false positive.In order to eliminate the impact of IgG, the mode of taking at present is to add anti-human IgG antibody in sample buffer, like this, keeps at normal temperatures easily causing antibody to lose efficacy.
During detection, sample need to be added drop-wise on sample pad with dropper, operation inconvenience, and, outside test strips is exposed to, sample easily outflow causes the pollution to environment, and to the pollution of sample itself.
The operations such as pasteur pipet is the absorption that is widely used at present a small amount of liquid such as cell experiment, clinical testing, clonogenic assay, shift or carry.There is a hollow capsule upper end of the body of pasteur pipet, the translucent brilliant white of body, and tube wall liquid fluidity ideal, easily the property controlled is strong.The very thin softness of pipe shaft, flexible, the convenient micro-special container of coming in and going out, there is long sharp mouth the body lower end, can guarantee the repeatability of the amount of dripping.
The utility model content
Technical problem to be solved in the utility model is to provide a kind of convenient kit of drawing the detection IgM of sample of avoiding polluting, eliminating the IgG impact.
The technical solution of the utility model is as follows: a kind of convenient kit of drawing the detection IgM of sample, it is characterized in that: comprise pasteur pipet (1) and be enclosed within the cap (3) on the sharp mouth of pasteur pipet (1), in described pasteur pipet (1), colloidal gold strip is housed, described colloidal gold strip comprises sample pad (2a), be coated with the gold mark pad (2b) of the anti-human IgM monoclonal antibody of colloid gold label, reaction film (2c), adsorptive pads (2d), be coated with IgG filtration membrane (2e) and the backer board (2f) of anti-human IgG antibody, described reaction film (2a) sticks on the upper surface of backer board (2f), described reaction film (2c) is provided with the detection line (T) that is coated with antigen and the nature controlling line (C) that is coated with anti-mouse IgG, one end of described gold mark pad (2b) is overlapped on the upper surface of reaction film (2c) end, the upper surface of the other end of described gold mark pad (2b) is overlapped with IgG filtration membrane (2e), described IgG filtration membrane (2e) is overlapped with sample pad (2a) away from the upper surface of an end of gold mark pad (2b), the upper surface of described reaction film (2c) other end is overlapped with adsorptive pads (2d), the adsorptive pads of wherein said colloidal gold strip (2d) is near the hollow capsule (1c) of the upper end of pasteur pipet (1), the sample pad of described colloidal gold strip (2a) is near the sharp mouth of pasteur pipet (1) lower end.
Adopt technique scheme, colloidal gold strip is contained in pasteur pipet, during detection, the hollow capsule of extruding pasteur pipet upper end, then be immersed in the pipette tip mouth under the sample liquid level, loosen hollow capsule and draw sample, after sucking a certain amount of sample, suction pipe is taken out from sample, continued to loosen allowing sample enter sample pad, then cover cap, timing, observations.Saved the plastic casing of kit, saved the process of drawing sample and dripping sample, operated easier, avoided drawing and drip in the sample process pollution to sample, increase cap, outside sample pad is not exposed to, avoided the pollution on the environment that spills of sample.Pasteur pipet bottom point mouth is thinner, can control the speed of drawing sample, and when sample size is less, thinner sharp mouth can be drawn sample as far as possible.
In addition: anti-human IgG antibody is coated on the IgG filtration membrane, and anti-human IgG antibody is difficult for losing efficacy, and more easily preserves than being added in sample buffer, and IgG can be eliminated or IgG concentration is reduced to the level can't detect, and has improved the accuracy rate of IgM antibody test.
In technique scheme: described pasteur pipet (1) is comprised of upper tube body (1a) and lower tube body (1b), the upper end of described upper tube body (1) is provided with hollow capsule (1c), the bottom of described upper tube body (1a) is provided with external thread, the top of described lower tube body (1b) is provided with internal thread, the tube wall of described lower tube body (1b) is provided with maximum scale line (1d), the bottom of described lower tube body (1b) is sharp mouth, described upper tube body (1a) and lower tube body (1b) are threaded, the sample pad of described colloidal gold strip (2a) is positioned at the top of maximum scale line (1d).Like this upper tube body and lower tube body employing are threaded, can pack into easily and take out test strips.
In technique scheme: described reaction film (2c), IgG filtration membrane (2e) are made by nitrocellulose membrane.
In technique scheme: described adsorptive pads (2d) is made by filter paper.
Beneficial effect: the utility model is reasonable in design, simple in structure, enforcement is easy, have easy, quick, highly sensitive and specificity is high, economical and practical, the convenient sample of drawing, be difficult for causing the pollution to environment of sample contamination and sample, can eliminate the impact of IgG on IgM, improve the accuracy rate of IgM antibody test.
The accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
The explosive view that Fig. 2 is pasteur pipet;
The structural representation that Fig. 3 is colloidal gold strip.
Embodiment
Below in conjunction with drawings and Examples, the utility model is described in further detail:
As Figure 1-3, the convenient kit of drawing the detection IgM of sample of the present utility model comprises pasteur pipet 1 and is enclosed within the cap 3 on the sharp mouth of pasteur pipet 1.Described cap 3 entangles the sharp mouth integral body of pasteur pipet 1.The one-piece construction of described pasteur pipet 1 is identical with existing pasteur pipet, different, and the utility model is partly therefrom upper tube body 1a and lower tube body 1b by pasteur pipet 1.The upper end of described upper tube body 1a is provided with hollow capsule 1c, and the bottom of described upper tube body 1a is provided with external thread, and wherein the caliber of external thread section is slightly less than the caliber of upper tube body 1a.That is to say, close up in the lower end of described upper tube body 1a, on the section that closes up, is provided with external thread.The top of described lower tube body 1b is provided with internal thread, and the tube wall of described lower tube body 1b is provided with maximum scale line 1d, and the bottom of described lower tube body 1b is long sharp mouth.Described upper tube body 1a and lower tube body 1b are threaded,
Described colloidal gold strip comprises sample pad 2a, be coated with the gold mark pad 2b of the anti-human IgM monoclonal antibody of colloid gold label, reaction film 2c, adsorptive pads 2d and backer board 2f, described reaction film 2c sticks on the upper surface of backer board 2f, described reaction film 2c is provided with detection line T and nature controlling line C, the end of described gold mark pad 2b is overlapped on the upper surface of the end of reaction film 2c, the other end of described gold mark pad 2b stretches out outside reaction film 2c, the upper surface of the external part of described gold mark pad 2b is overlapped with IgG filtration membrane 2e, the end of described IgG filtration membrane 2e is overlapped on gold mark pad 2b, the other end stretches out outside gold mark pad 2b, the upper surface of described IgG filtration membrane 2e external part is overlapped with sample pad 2a, the upper surface of the other end of described reaction film 2c is overlapped with adsorptive pads 2d, the end of described adsorptive pads 2d is overlapped on reaction film 2c, the other end stretches out outside reaction film 2c, described reaction film 2c, IgG filtration membrane 2e makes by nitrocellulose membrane.Described adsorptive pads 2d is made by filter paper.
The sizableness of the diameter of the width of the backer board 2f of described colloidal gold strip and pasteur pipet 1 body, the colloidal gold strip pasteur pipet 1 of packing into can not rock when interior like this.While packing into, first upper tube body 1a and lower tube body 1b are turned on, colloidal gold strip is vertically packed in pasteur pipet 1, first by the upper tube body 1a that packs into of colloidal gold strip top (adsorptive pads 2d upward), again the bottom of colloidal gold strip is inserted in lower tube body 1b, then upper tube body 1a and lower tube body 1b are tightened, that is to say that adsorptive pads 2d is near hollow capsule 1c, the sample pad 2a of described colloidal gold strip is positioned at the sharp mouth of lower end near pasteur pipet 1, and described sample pad 2a is positioned at maximum scale line 1d top.
Anti-human IgG antibody is coated on to IgG filtration membrane 2e upper, anti-human IgG antibody is difficult for losing efficacy, and more easily preserves than being added in sample buffer, and IgG can be eliminated or IgG concentration is reduced to the level can't detect, and has improved the accuracy rate of IgM antibody test.
During detection, the hollow capsule 1c of extruding pasteur pipet 1, then the sharp mouth of pasteur pipet 1 is immersed under the sample liquid level, loosen hollow capsule 1c and draw sample, when sample reaches the maximum scale line 1d of pasteur pipet 1, pasteur pipet 1 is taken out from sample, continue to loosen and allow sample enter sample pad 2a, then cover cap 3, timing, observations.Saved the plastic casing of kit, saved the process of drawing sample and dripping sample, operate easylier, avoided drawing and drip in the sample process pollution to sample, increase cap 3, avoided the pollution on the environment that spills of sample.Pasteur pipet 1 bottom point mouth is thinner, can control the speed of drawing sample, and when sample size is less, thinner sharp mouth can be drawn sample as far as possible.
Claims (4)
1. a convenient kit of drawing the detection IgM of sample, it is characterized in that: comprise pasteur pipet (1) and be enclosed within the cap (3) on the sharp mouth of pasteur pipet (1), in described pasteur pipet (1), colloidal gold strip is housed, described colloidal gold strip comprises sample pad (2a), be coated with the gold mark pad (2b) of the anti-human IgM monoclonal antibody of colloid gold label, reaction film (2c), adsorptive pads (2d), be coated with IgG filtration membrane (2e) and the backer board (2f) of anti-human IgG antibody, described reaction film (2a) sticks on the upper surface of backer board (2f), described reaction film (2c) is provided with the detection line (T) that is coated with antigen and the nature controlling line (C) that is coated with anti-mouse IgG, one end of described gold mark pad (2b) is overlapped on the upper surface of reaction film (2c) end, the upper surface of the other end of described gold mark pad (2b) is overlapped with IgG filtration membrane (2e), described IgG filtration membrane (2e) is overlapped with sample pad (2a) away from the upper surface of an end of gold mark pad (2b), the upper surface of described reaction film (2c) other end is overlapped with adsorptive pads (2d), the adsorptive pads of wherein said colloidal gold strip (2d) is near the hollow capsule (1c) of the upper end of pasteur pipet (1), and the sample pad of described colloidal gold strip (2a) is near the sharp mouth of pasteur pipet (1) lower end.
2. facilitate according to claim 1 the kit of the detection IgM that draws sample, it is characterized in that: described pasteur pipet (1) is comprised of upper tube body (1a) and lower tube body (1b), the upper end of described upper tube body (1a) is provided with hollow capsule (1c), the bottom of described upper tube body (1a) is provided with external thread, the top of described lower tube body (1b) is provided with internal thread, the tube wall of described lower tube body (1b) is provided with maximum scale line (1d), the bottom of described lower tube body (1b) is sharp mouth, described upper tube body (1a) and lower tube body (1b) are threaded, the sample pad of described colloidal gold strip (2a) is positioned at the top of maximum scale line (1d).
3. according to the described convenient kit of drawing the detection IgM of sample of claim 1 or 2, it is characterized in that: described reaction film (2c), IgG filtration membrane (2e) are made by nitrocellulose membrane.
4. facilitate according to claim 1 the kit of the detection IgM that draws sample, it is characterized in that: described adsorptive pads (2d) is made by filter paper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320626797.7U CN203385735U (en) | 2013-10-11 | 2013-10-11 | Kit for conveniently sucking sample and detecting IgM |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320626797.7U CN203385735U (en) | 2013-10-11 | 2013-10-11 | Kit for conveniently sucking sample and detecting IgM |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203385735U true CN203385735U (en) | 2014-01-08 |
Family
ID=49874211
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320626797.7U Expired - Fee Related CN203385735U (en) | 2013-10-11 | 2013-10-11 | Kit for conveniently sucking sample and detecting IgM |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203385735U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106290912A (en) * | 2016-08-12 | 2017-01-04 | 泰州泽成生物技术有限公司 | A kind of IgM antibody detection adsorbent and preparation method thereof |
-
2013
- 2013-10-11 CN CN201320626797.7U patent/CN203385735U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106290912A (en) * | 2016-08-12 | 2017-01-04 | 泰州泽成生物技术有限公司 | A kind of IgM antibody detection adsorbent and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11105798B2 (en) | Device and method for capillary chemiluminescence detection | |
| CN101036056A (en) | Reaction vessel | |
| CN209167324U (en) | It is a kind of to detect the detecting device integrated of helicobacter pylori in fecal specimens | |
| CN103316883B (en) | Wash trigger waste liquid discharge device | |
| CN203385735U (en) | Kit for conveniently sucking sample and detecting IgM | |
| CN104568511A (en) | Medical examination sampling device | |
| CN204177806U (en) | A kind of immunity-chromatography test strip | |
| CN203385591U (en) | Kit for conveniently sucking sample | |
| CN207248887U (en) | Canine parvovirus time-resolved fluoroimmunoassay chromatographs detection kit | |
| CN103656764A (en) | Improved negative-pressure aspirator | |
| CN206906064U (en) | A kind of HIV mouth check reagent boxes for carrying sampler | |
| CN201251579Y (en) | Special test tube for a full automatic biochemical immunity analyzer | |
| CN209784383U (en) | Chemiluminescence immunodetection system | |
| CN104226384B (en) | Liquid-discharging means of bottle | |
| CN206725450U (en) | A kind of measure device of soil alkali-hydrolyzable nitrogen | |
| CN2789768Y (en) | Automatic filling liquid micro-burette apparatus | |
| CN208583356U (en) | The dedicated liquid getting device of reagent | |
| CN206027164U (en) | Gas -liquid separating device | |
| CN203385722U (en) | IgM (Immunoglobulin M) detection test strip capable of eliminating IgG (Immunoglobulin G) interference | |
| CN206152825U (en) | Novel acid type burette | |
| CN206146678U (en) | Liquid drug sampling pipe that conveniently carries and deposit | |
| CN203385737U (en) | Colloidal gold test strip for detecting IgM (Immunoglobulin M) | |
| CN219331724U (en) | 24-hour urine protein specimen sampling tube | |
| CN202279285U (en) | Disposable bottle for containing liquid | |
| CN209979646U (en) | Enzyme-linked immunoassay kit for detecting toxins in food |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140108 Termination date: 20171011 |