CN203354686U - Mouse bile collection device - Google Patents

Mouse bile collection device Download PDF

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Publication number
CN203354686U
CN203354686U CN 201320478342 CN201320478342U CN203354686U CN 203354686 U CN203354686 U CN 203354686U CN 201320478342 CN201320478342 CN 201320478342 CN 201320478342 U CN201320478342 U CN 201320478342U CN 203354686 U CN203354686 U CN 203354686U
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China
Prior art keywords
bile
mice
gallbladder
gathering
mouse
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Expired - Fee Related
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CN 201320478342
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Chinese (zh)
Inventor
赵进顺
邹宝波
林夏露
刘魁
岳霞
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Ningbo University
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Ningbo University
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Priority to CN 201320478342 priority Critical patent/CN203354686U/en
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Publication of CN203354686U publication Critical patent/CN203354686U/en
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Abstract

The utility model discloses a mouse bile collection device which comprises a fixing bracket. The mouse bile collection device is characterized in that at least one bile collection unit is connected with the fixing bracket; each bile collection unit comprises a mouse feeding cage and a bile collection device; the bile collection device comprises a liquid storage cup for storing bile and a gall bladder intubation tube of which one end is connected with the liquid storage cup and the other end is implanted into the mouse gall bladder; the gall bladder intubation tube is positioned at one end of the mouse gall bladder and is expanded to form an expanded end; the gall bladder intubation tube is bonded on a positioning iron wire which vertically drops down; the upper end of the positioning iron wire is movably connected with the fixing bracket; and the lower end of the positioning iron wire is fixed on the neck of a mouse. The mouse bile collection device is capable of collecting the mouse bile for a long period without affecting the ordinary activity of the mouse, is simple in structure, convenient to operate, safe and reliable and capable of collecting the mouse bile for a long period.

Description

A kind of mice bile gathering-device
Technical field
This utility model relates to the animal body fluid gathering-device, especially mice bile gathering-device.
Background technology
It is in giving medicine or nutrient that animal gallbladder intubate is collected the bile model, studies its absorption through liver, decomposition, metabolism model more accurately, in the animal experiment studies such as toxicology, pharmacology and threpsology, critical role is arranged.Thereby the current liver metabolism study general animal via cystic duct cannula relatively large with Canis familiaris L., rabbit and rat etc. collected the model structure of bile and obtained experimental data, only with relatively large animal, as experimental subject, can make experimental result have one-sidedness and inaccuracy; And when choosing meiofauna as experimental subject, tend to select mice, but the mice common bile duct is tiny, the intubate of existing experimental model is very easily damaged gallbladder and the common bile duct of mice, is unfavorable for long-term observation and in the situation that the free movable bile of collecting of mice.Can not affect the normal work and rest of mice if can utility model a kind of and can continue to collect again the gathering-device of mice bile, can effectively collect enough mice bile for experiment.Simultaneously, comparatively complicated during this device practical operation, should before the live body operation, carry out the operation of more time on mouse model.
Summary of the invention
Technical problem to be solved in the utility model is for above-mentioned prior art present situation, and provide a kind of mice bile gathering-device and the operational approach on mouse model thereof, this mice bile gathering-device can be implanted the interior bile of collection activity Mice Body in the mice gallbladder, it is convenient that this apparatus structure is simple, bile is collected, and can collect for a long time mice bile, this utility model disclosed by this application of installation the operational approach on mouse model, there is the effect that can effectively promote the proficiency of user.
This utility model solves the problems of the technologies described above adopted technical scheme:
A kind of mice bile gathering-device, include fixed support, wherein, fixed support is connected with at least one bile collector unit, each bile collector unit includes a mice rearging cage and a bile gathering-device, liquid storing cup and an end that the bile gathering-device includes for storing bile are connected with liquid storing cup, the other end is implanted the gallbladder intubate of mice gallbladder, the end that the gallbladder intubate is positioned at the mice gallbladder expands to be formed with and expands end, the gallbladder intubate is bundled on a vertical sagging location iron wire, iron wire upper end, location and fixed support are flexibly connected, its lower end is fixed on the mice cervical region.
Above-mentioned liquid storing cup also is fixedly connected with the location iron wire.
Above-mentioned fixed support consists of a suspension rod and spaced montant, each bile collector unit all with corresponding montant secure fit.
Be arranged at intervals with rotating shaft on above-mentioned suspension rod, rotating shaft is connected with the location iron wire.
Above-mentioned gallbladder intubate is crooked sagging and be placed in liquid storing cup in the lower end of rotating shaft.
Between above-mentioned liquid storing cup and described location iron wire, by lashing wire, be connected and fixed.
Be provided with the drinking-water cylinder in above-mentioned mice rearging cage, the lower end of drinking-water cylinder is provided with drinking tube.
A kind of operational approach of using mice bile gathering-device on mouse model comprises the following steps:
Step 1, preparation mouse model, be provided with the gallbladder model in proportion in the mouse model abdomen;
Step 2, prepare the gallbladder intubate, straight type polyurethane conduit is elongated in the water of 80 ℃ and make long S type structure, then the polyurethane catheter of S type one end is melted on the electric cautery of 100 ℃ to formation and expand end, make the gallbladder intubate;
Step 3, mouse model is lain on the back on operating board, expose the gallbladder model in the mouse model abdomen, fix a fast knot in gallbladder model middle and lower part, subsequently the end that expands of gallbladder intubate is implanted in the gallbladder model, and immediately by suture loop around fixing, confirm expanding after end has been positioned at the gallbladder model of gallbladder intubate, mix up the position of gallbladder intubate, close the mouse model abdominal cavity, and the gallbladder intubate is drawn from the mouse model cervical region;
Step 4, the gallbladder intubate of drawing and location iron wire are connected and fixed;
Step 5, will complete the mouse model that the gallbladder intubate implants and put into the mice rearging cage, the location iron wire will be connected with the rotating shaft on fixed support, and then liquid storing cup will be fixed on and locate on iron wire, then the outlet of gallbladder intubate upper end is placed in liquid storing cup.
The length of above-mentioned straight type polyurethane conduit is 15cm, and internal diameter is≤1mm.
In above-mentioned step 3, adopt suture that a fast knot is fixed in gallbladder model middle and lower part, and suture is No. 6 sutures.
Compared with prior art, a kind of mice bile gathering-device of the present utility model, include fixed support, wherein, fixed support is connected with at least one bile collector unit, each bile collector unit includes a mice rearging cage and a bile gathering-device, liquid storing cup and an end that the bile gathering-device includes for storing bile are connected with liquid storing cup, the other end is implanted the gallbladder intubate of mice gallbladder, the end that the gallbladder intubate is positioned at the mice gallbladder expands to be formed with and expands end, be connected with a vertical sagging location iron wire on the gallbladder intubate, rotating shaft on location iron wire one end and fixed support is flexibly connected, the other end is fixed on the nape section of mice.This device can be implanted the mice gallbladder by the gallbladder intubate, and mice is put into to the mice rearging cage and let alone free activity, in the situation that do not affect the mice activity, complete the collection of mice bile, can, for the collection of long-term mice bile, can also carry out the bile collection to many mices simultaneously.Simple in structure with the mouse model that this device is complementary, can allow the user of this device just can obtain enough use proficiencys before live test, improve the probability of successful surgery.
The accompanying drawing explanation
Fig. 1 is the structural representation of this utility model embodiment;
Fig. 2 is the structural representation of bile collector unit in Fig. 1;
Fig. 3 is I section enlarged drawing in Fig. 2.
The specific embodiment
Below in conjunction with accompanying drawing, embodiment is described in further detail this utility model.
Be structural representation of the present utility model as shown in Figure 1 to Figure 3,
Reference numeral wherein is: fixed support 1, suspension rod 11, montant 12, rotating shaft 13, connection tube 14, mice rearging cage 2, location iron wire 3, gallbladder intubate 4, expand end 41, liquid storing cup 5, lashing wire 6, drinking-water cylinder 7, drinking tube 71.
As shown in Figure 1 to Figure 3,
A kind of mice bile gathering-device, include fixed support 1, wherein, fixed support 1 is connected with several bile collector units, each bile collector unit includes a mice rearging cage 2 and a bile gathering-device, liquid storing cup 5 and an end that the bile gathering-device includes for storing bile are connected with liquid storing cup 5, the other end is implanted the gallbladder intubate 4 of mice gallbladder, the end that gallbladder intubate 4 is positioned at the mice gallbladder expands to be formed with and expands end 41, gallbladder intubate 4 is bundled on a vertical sagging location iron wire 3, location iron wire 3 one ends and fixed support 1 are flexibly connected, lower end is fixed on the mice cervical region.
In embodiment, liquid storing cup 5 is fixedly connected with location iron wire 3.
In embodiment, fixed support 1 consists of a suspension rod 11 and spaced montant 12, each bile collector unit all with corresponding montant 12 secure fit.The bile collector unit is arranged side by side, compartment of terrain is arranged on described fixed support 1; A plurality of bile collector units are set up in parallel, and can carry out the collection of bile to a plurality of mices simultaneously, to increase the collection of experimental data, increase the comparative between experimental data, thereby improve the accuracy of experiment.
In embodiment, be arranged at intervals with rotating shaft 13 on suspension rod 11, the lower end of rotating shaft 13 is provided with connection tube 14, and the end that location iron wire 3 is flexibly connected with fixed support 1 is through this connection tube 14 and be connected cooperation with it.
In embodiment, gallbladder intubate 4 is crooked sagging and be placed in liquid storing cup 5 in the lower end of rotating shaft 13.
In embodiment, between liquid storing cup 5 and described location iron wire 3, by lashing wire 6, be connected and fixed.
In embodiment, be provided with drinking-water cylinder 7 in mice rearging cage 2, the lower end of drinking-water cylinder 7 is provided with drinking tube 71.
Comparatively complicated during device practical operation of the present utility model, should before the live body operation, carry out the operation of more time on mouse model, therefore, the utility model discloses a kind of operational approach of using mice bile gathering-device on mouse model, comprise the following steps:
Step 1, preparation mouse model, be provided with the gallbladder model in proportion in the mouse model abdomen;
Step 2, preparation gallbladder intubate 4 are elongated straight type polyurethane conduit and are made long S type structure in the water of 80 ℃, then the polyurethane catheter of S type one end are melted on the electric cautery of 100 ℃ to form to expand and hold 41, make gallbladder intubate 4;
Step 3, mouse model is lain on the back on operating board, expose the gallbladder model in the mouse model abdomen, fix a fast knot in gallbladder model middle and lower part, subsequently the end 41 that expands of gallbladder intubate 4 is implanted in the gallbladder model, and use immediately suture loop around fixing, after confirming that expanding of gallbladder intubate 4 holds 41 to be positioned at the gallbladder model, mix up the position of gallbladder intubate 4, close the mouse model abdominal cavity, and gallbladder intubate 4 is drawn from the mouse model cervical region;
Step 4, gallbladder intubate 4 and the location iron wire 3 of drawing are connected and fixed;
Step 5, will complete the mouse model that the gallbladder intubate implants and put into the mice rearging cage, the location iron wire will be connected with the rotating shaft on fixed support, and then liquid storing cup will be fixed on and locate on iron wire, then the outlet of gallbladder intubate upper end is placed in liquid storing cup.The activity of simulation mice, adjust the location iron wire until the mouse model activity is not hindered.
In embodiment, the length of straight type polyurethane conduit is 15cm, and internal diameter is≤1mm.
In embodiment, in step 3, adopt suture that a fast knot is fixed in gallbladder model middle and lower part, and suture is No. 6 sutures.
First, at the enterprising line operate of mouse model, can effectively promote the proficiency of user, improve the success rate of live body operation.
While on intravital mouse, doing experiment, method is different again, and concrete grammar comprises the following steps:
The making of step 1, gallbladder intubate 4: be 15cm by length, the gallbladder intubate 4 of making long S type structure is elongated at the two ends of the straight type polyurethane conduit that internal diameter is 1mm in the water of 80 ℃ with a conduit made of iron, then the one end is melted on the electric cautery of 100 ℃ one expand the end 41;
Step 2, the implantation of gallbladder intubate 4: be that 1.5% to 5% isoflurane carries out inhalation anesthesia to mice by concentration expressed in percentage by weight, mice is lain on the back on the operation thermostatic platform, smear the hair of mouse web portion with suds, cut off this part hair with razor again and carry out preserved skin, after the skin of sterilizing with medical alcohol, do longitudinal cut with scalpel, expose the liver of mice and the gallbladder of its below, after the ligation common bile duct, fully expose gallbladder, and thereunder pad a gauze, fix a fast knot with No. 6 sutures in the gallbladder middle and lower part, subsequently the end 41 that expands of gallbladder intubate 4 is implanted in the gallbladder of mice, and use immediately suture loop around fixing, after confirming that bile flows into gallbladder intubate 4 smoothly, mix up the position of gallbladder intubate 4, close abdominal cavity, and gallbladder intubate 4 is drawn from the mice cervical region through subcutaneous,
Fixing of step 3, gallbladder intubate 4: gallbladder intubate 4 and the location iron wire 3 of drawing are connected and fixed;
The collection of step 4, bile: the mice that will complete 4 implantation of gallbladder intubate is put into mice rearging cage 2, then the outlet of gallbladder intubate 4 upper ends is placed in liquid storing cup 5.Adjust the position of location iron wire 3, appoint mice freely movable, allow the bile of mice gallbladder secretion flow into liquid storing cup 5 through gallbladder intubate 4, complete the collection of bile.
Fixed support 1 consists of a suspension rod 11 and the spaced montant 12 of Duo Gen, on every montant 12, can connect a mice rearging cage 2, the interior setting of mice rearging cage 2 at a draft water drum 7 for to mice, providing drinking-water; In the bile collection process, be sprinkled into food in mice rearging cage 2, and at the interior injection drinking-water of drinking-water cylinder 7.The mice that completes 4 implant surgeries of gallbladder intubate is raised in this mice rearging cage 2, gallbladder intubate 4 is connected cooperation with sagging location iron wire 3, the 4 outlet bendings of gallbladder intubate are placed in liquid storing cup 5, the upper end of location iron wire 3 is connected cooperation through the connection tube 14 of rotating shaft 13 lower ends settings with the suspension rod 11 of fixed support 1, just due to the setting of rotating shaft 13, and 30 minutes softnesses of location iron wire, mice is in the mice rearging cage, location iron wire 3 and gallbladder intubate 4 all can not affect the activity of mice, both guaranteed the normal collection of the bile of mice, do not affect again the normal drinking-water of mice, search for food and activity.
The setting that end 41 is expanded in gallbladder intubate 4 ends, effectively prevent that mice is when activity, and gallbladder intubate 4 can not extracted from gallbladder, guarantees normally carrying out of bile collection; And gallbladder intubate 4 is after iron wire 3 is connected with location, and gallbladder intubate 4 and liquid storing cup 5 be the top in mice rearging cage 2 all, touches while avoiding the mice activity and affect the collection of bile.
Use mice bile gathering-device of the present utility model to carry out the collection of mice bile, can be in the situation that do not affect the mice activity, carrying out long-term bile collects, when collecting bile, can also carry out real-time dynamic observation to mice, thereby obtain experimental data accurately, for the carrying out of research.
Most preferred embodiment of the present utility model is illustrated, and the various variations of being made by those of ordinary skills or remodeling can not break away from scope of the present utility model.

Claims (7)

1. a mice bile gathering-device, include fixed support (1), it is characterized in that: described fixed support (1) is connected with at least one bile collector unit, each described bile collector unit includes a mice rearging cage (2) and a bile gathering-device, liquid storing cup (5) and an end that described bile gathering-device includes for storing bile are connected with liquid storing cup (5), the other end is implanted the gallbladder intubate (4) of mice gallbladder, the end that described gallbladder intubate (4) is positioned at the mice gallbladder expands to be formed with and expands end (41), described gallbladder intubate (4) is bundled on a vertical sagging location iron wire (3), iron wire (3) upper end, described location and fixed support (1) are flexibly connected, its lower end is fixed on the mice cervical region.
2. a kind of mice bile gathering-device according to claim 1 is characterized in that: described liquid storing cup (5) is fixedly connected with location iron wire (3).
3. a kind of mice bile gathering-device according to claim 2, it is characterized in that: described fixed support (1) consists of a suspension rod (11) and spaced montant (12), each described bile collector unit all with montant (12) secure fit accordingly.
4. a kind of mice bile gathering-device according to claim 3, it is characterized in that: be arranged at intervals with rotating shaft (13) on described suspension rod (11), described rotating shaft is connected with described location iron wire.
5. a kind of mice bile gathering-device according to claim 4 is characterized in that: described gallbladder intubate (4) is crooked sagging and be placed in liquid storing cup (5) in the lower end of rotating shaft (13).
6. a kind of mice bile gathering-device according to claim 5, is characterized in that: between described liquid storing cup (5) and described location iron wire (3), by lashing wire (6), be connected and fixed.
7. a kind of mice bile gathering-device according to claim 6 is characterized in that: be provided with drinking-water cylinder (7) in described mice rearging cage (2), the lower end of described drinking-water cylinder (7) is provided with drinking tube (71).
CN 201320478342 2013-08-07 2013-08-07 Mouse bile collection device Expired - Fee Related CN203354686U (en)

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CN 201320478342 CN203354686U (en) 2013-08-07 2013-08-07 Mouse bile collection device

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Application Number Priority Date Filing Date Title
CN 201320478342 CN203354686U (en) 2013-08-07 2013-08-07 Mouse bile collection device

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112082474A (en) * 2019-06-12 2020-12-15 哈里森家禽有限公司 System and method for automatic extraction
CN112971864A (en) * 2021-05-14 2021-06-18 中国医学科学院医学实验动物研究所 Animal brain microdialysis recording device for waking and freely moving

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112082474A (en) * 2019-06-12 2020-12-15 哈里森家禽有限公司 System and method for automatic extraction
CN112971864A (en) * 2021-05-14 2021-06-18 中国医学科学院医学实验动物研究所 Animal brain microdialysis recording device for waking and freely moving

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CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20131225

Termination date: 20160807