CN203350254U

CN203350254U - Immunohistochemical kit

Info

Publication number: CN203350254U
Application number: CN 201320386805
Authority: CN
Inventors: 金昕; 黄丹; 赵应斌; 乔琳琼; 罗翠; 王新星
Original assignee: WUHAN SANYING BIOTECHNOLOGY Co Ltd
Current assignee: WUHAN SANYING BIOTECHNOLOGY Co Ltd
Priority date: 2013-07-02
Filing date: 2013-07-02
Publication date: 2013-12-18
Anticipated expiration: 2023-07-02

Abstract

The utility model relates to an immunohistochemical kit. Conventional immunohistochemical experiment reagents required by the biological or medical laboratory at present are usually self-weighed and self-made or purchased from outside. The immunohistochemical kit is provided with reagents required by the conventional immunohistochemical experiment, is convenient to use and simple to operate, saves both time and labor, and enhances the work efficiency. The kit comprises a box body, a box cover, an instruction book, a first partition, a second partition, a citric acid restoration powder, a ready-to-use peroxidase inhibitor, a ready-to-use BSA (bovine serum albumin) sealing fluid, a ready-to-use HRP (horse radish peroxidase) label secondary antibody, a first DAB diluent, a second DAN diluent, a DAB concentrated solution, a ready-to-use brazilein staining solution, a first TBS powder and a second TBS powder.

Description

The SABC kit

Technical field

The utility model relates to a kind of immunohistochemical assay kit.

Background technology

Immunohistochemistry is to utilize the specific binding of antigen-antibody, by the colour developing of label, detect with position tissue or cell in certain chemical substance.In immunohistochemical assay, need plurality of reagents to be used in conjunction with, this kit has combined required reagent in the experiment of routine immunization group.

Summary of the invention

The utility model provides a kind of agent combination comprehensive SABC kit for the experiment of routine immunization group, easy to use, simple to operate, time saving and energy saving, can increase work efficiency.

The technical solution of the utility model is: a kind of kit of routine immunization group experiment use, comprise box body and lid, 8 bottles of reagent and fixing the first dividing plate of bottle, the second partition of 2 bags of packed reagent and fixing packed reagent, a instructions, 8 bottles of reagent are respectively the 3.4g citric acid and repair pulvis, 5ml instant superoxide enzyme inhibitor, 5ml instant BSA confining liquid, 5ml instant HRP mark two is anti-, 12.5ml the one DAB dilution, 12.5ml the 2nd DAB dilution, 0.5ml the DAB concentrate, 5ml instant haematoxylin dyeing liquid.2 bags of pulvis are 22g the one TBS pulvis, 22g the 2nd TBS pulvis.Before immunohistochemical experiment, the pulvis by specification is made into to required reagent, can use.The use of enough 100 sections of reagent in kit.The utility model has the advantages that: detect agents useful for same comprehensive, without weighing preparation or purchase voluntarily separately, easy to use, simple to operate, time saving and energy saving, increase work efficiency.

The accompanying drawing explanation

Accompanying drawing is the structural representation after the utility model opens the lid.

In figure: 1. box body, 2. lid, 3a. the first dividing plate, the 3b. second partition,

4. citric acid is repaired pulvis, 5. instant superoxide enzyme inhibitor, 6. instant BSA confining liquid, 7. instant HRP mark two is anti-, 8a. the one DAB dilution, 8b. the 2nd DAB dilution, 9.DAB concentrate, 10. instant haematoxylin dyeing liquid, 11a. the one TBS pulvis, 11b. the 2nd TBS pulvis.

Embodiment

Below in conjunction with drawings and Examples, the utility model is described in detail.

Referring to accompanying drawing: box body (1) is the cube carton box, the first dividing plate (3a) is the cardboard of white with 8 recessed bottle positions, and second partition (3b) is white atresia cardboard, and the first dividing plate (3a) is on box body top, second partition (3b) is in the box body bottom, and two dividing plates are all detachable.3.4g it is the white PE plastic bottle of white cap that citric acid is repaired pulvis (4), 5ml instant superoxide enzyme inhibitor (5) is the black PE plastic bottle of red cap, 5ml instant BSA confining liquid (6) is the black PE plastic bottle of blue cap, 5ml instant HRP mark two anti-(7) is the white PE plastic bottle of yellow cap, 12.5ml a DAB dilution (8a) is the white PE plastic bottle of black caps, 12.5ml the 2nd DAB dilution (8b) is the white PE plastic bottle of black caps, 0.5mlDAB concentrate (9) is the black PE plastic bottle of black caps, 5ml instant haematoxylin dyeing liquid (10) is the white PE plastic bottle of green cap, 22g the one TBS pulvis (11a) is transparent PE plastics valve bag, 22g the 2nd TBS pulvis (11b) is transparent PE plastics valve bag.The first dividing plate (3a) has 8 recessed bottle positions, placement location is successively: the 3.4g citric acid is repaired pulvis in bottle position (4), 5ml instant superoxide enzyme inhibitor is in bottle position (5), 5ml instant BSA confining liquid is in bottle position (6), 5ml instant HRP mark two resists in bottle position (7), and 12.5ml the one DAB dilution is at bottle position (8a), and 12.5ml the 2nd DAB dilution is at bottle position (8b), 0.5mlDAB concentrate is in bottle position (9), 5ml instant haematoxylin dyeing liquid is in bottle position (10).22g the one TBS pulvis (11a) and 22g the 2nd TBS pulvis (11b) are placed in room under second partition (3b).

The specific implementation process of the present embodiment:

1, the dewaxing treatment of paraffin section

1) at dimethylbenzene (I) with (II), respectively soak 15 minutes.

2) at absolute ethyl alcohol, 95% ethanol, 80% ethanol and 60% ethanol, respectively soak successively 5 minutes.

3) in single steaming water, soak 5 minutes.

2, antigen retrieval

The 3.4g citric acid is repaired to pulvis (4) and with single water that steams, be mixed with 1L and repair liquid (1X), get in right amount in a high-temperature resistant container, section is put into wherein, then container is put into to micro-wave oven, moderate heat heats 5-15 minute.Complete, be placed on the cooling 30-40 minute of room temperature.

3, immunohistochemical staining

1) 44gTBS pulvis (11a, 11b) is mixed with to 4LTBS solution (1X) with single water that steams, regulates pH value to 7.6.

2) cooling section is taken out, rinse 4-5 time with TBS, each 30sec, dry, and cleans, and adds appropriate instant superoxide enzyme inhibitor (5), and in the wet box of room temperature, sealing is 10 minutes.

3) TBS rinses 4-5 time, and each 30sec, dry, and cleans, and drips appropriate instant BSA confining liquid (6), the wet box sealing of room temperature 1 hour.

4) suck unnecessary liquid with thieving paper, drip appropriate primary antibodie, the wet box of room temperature is hatched 1 hour or 4 ℃ of wet box overnight incubation.

5) TBS rinses 4-5 time, and each 30sec drips appropriate instant HRP mark two anti-(7), and the wet box of room temperature is hatched 30 minutes.

6) preparation DAB nitrite ion, every 5ulDAB concentrate (9) adds DAB dilution (8a, 8b) 250ul.TBS rinses 4-5 time, and each 30sec, drip the DAB configured in right amount, and within 2-5 minute, post-flush is clean.

7) drip 1 instant haematoxylin dyeing liquid (10), redye 2-3 minute, TBS rinses well, soaks 5-10 minute in tap water.

8) at 60% ethanol, 80% ethanol, 95% ethanol and absolute ethyl alcohol, soak 5 minutes successively, at dimethylbenzene (III) with (IV), respectively soaks 5 minutes.

9) neutral gum mounting, observations under microscope.

Claims

1. a SABC kit, comprise box body (1) and lid (2), 8 bottles of reagent and fixing first dividing plate (3a) of bottle, the second partition (3b) of 2 bags of packed reagent and fixing packed reagent, a instructions, 8 bottles of reagent are respectively citric acid and repair pulvis (4), instant superoxide enzyme inhibitor (5), instant BSA confining liquid (6), instant HRP mark two anti-(7), the one DAB dilution (8a) and the 2nd DAB dilution (8b), DAB concentrate (9), instant haematoxylin dyeing liquid (10), two bags of pulvis are a TBS pulvis (11a) and the 2nd TBS pulvis (11b).

2. SABC kit according to claim 1, it is characterized in that: box body (1) is the cube carton box, the first dividing plate (3a) is the cardboard of white with 8 recessed bottle positions, second partition (3b) is white atresia cardboard, it is the white PE plastic bottle of white cap that citric acid is repaired pulvis (4), instant superoxide enzyme inhibitor (5) is the black PE plastic bottle of red cap, instant BSA confining liquid (6) is the black PE plastic bottle of blue cap, instant HRP mark two anti-(7) is the white PE plastic bottle of yellow cap, the one DAB dilution (8a) is the white PE plastic bottle of black caps, the 2nd DAB dilution (8b) is the white PE plastic bottle of black caps, DAB concentrate (9) is the black PE plastic bottle of black caps, instant haematoxylin dyeing liquid (10) is the white PE plastic bottle of green cap, the one TBS pulvis (11a) is transparent PE plastics valve bag, the 2nd TBS pulvis (11b) is transparent PE plastics valve bag.

3. SABC kit according to claim 1 is characterized in that: 8 bottles of reagent are that to be inserted in the first dividing plate (3a) upper, and 2 bags of TBS pulvis are to be pressed under second partition (3b), and citric acid is repaired 1 bottle, pulvis, 3.4g; 1 bottle of instant superoxide enzyme inhibitor, 5ml; 1 bottle of instant BSA confining liquid, 5ml; Instant HRP mark two resists 1 bottle, 5ml; The one 1 bottle of DAB dilution (8a), 12.5ml; The 2nd 1 bottle of DAB dilution (8b), 12.5ml; 1 bottle of DAB concentrate, 0.5ml; 1 bottle of instant haematoxylin dyeing liquid, 5ml; The one 1 bag, TBS pulvis (11a), 22g; The 2nd 1 bag, TBS pulvis (11b), 22g.