CN203320013U - Plant homogenizing device - Google Patents
Plant homogenizing device Download PDFInfo
- Publication number
- CN203320013U CN203320013U CN201320314748XU CN201320314748U CN203320013U CN 203320013 U CN203320013 U CN 203320013U CN 201320314748X U CN201320314748X U CN 201320314748XU CN 201320314748 U CN201320314748 U CN 201320314748U CN 203320013 U CN203320013 U CN 203320013U
- Authority
- CN
- China
- Prior art keywords
- homogenate
- plant
- base
- homogenizing
- homogenizer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Abstract
The utility model relates to equipment for grinding a plant sample, and in particular relates to a plant homogenizing device. The plant homogenizing device comprises a base, a homogenizing pipe and a homogenizing bar, wherein a cylindrical hole in which the homogenizing pipe is arranged is formed in the base; the homogenizing bar is inserted into the homogenizing pipe; threads are formed at the front end of the homogenizing bar according to the design; the homogenizing bar is rotated to grind plant cell tissues in the homogenizing pipe. The plant homogenizing device has the characteristics of being made of a stainless steel material, wide in a temperature resistance range and capable of quenching; the plant cell tissues are grinded after the base, the homogenizing pipe and the homogenizing bar are pre-cooled in an refrigerator at -20 DEG C/-70 DEG C, and thus the degradation rate of nucleic acid can be reduced.
Description
Technical field
The utility model relates to the equipment that grinds plant sample, and specifically a kind of plant homogenizer, be applicable to the grinding of plant cell tissue, especially is applicable to the plant sample of needs cryogrinding.
Background technology
The grinding of plant tissue is first step of extracting plant nucleic acid, and the height of Grinding Quality directly has influence on concentration and the quality of nucleic acid extraction.Existing abrasive tool mainly is divided into mortar and the large class of homogenizer two, and mortar is generally used for the method for liquid nitrogen grinding and grinds plant cell tissue, and homogenizer is generally used for grinding the cell tissue of animal.Plant cell wall is difficult for broken, cell tissue hardening after liquid nitrogen freezing, and fragility increases, and is easy to grind; But the temperature of liquid nitrogen is-196 ℃, and temperature is extremely low, the improper frostbite phenomenon that just there will be is slightly arranged in operating process, there is certain danger coefficient.At present, laboratory adopts the homogenizer of glass material to grind zooblast tissue and cell walls and more breakable plant cell tissue usually, because glass is stressed little, temperature resistant range is-20 ℃-100 ℃, suddenly cold and hot is no more than 30 ℃, in process of lapping, the slightly large or homogenizer precooling in the refrigerator of-20 ℃/-70 ℃ of dynamics, all can make homogenizer cracked in process of lapping.
Summary of the invention
For the problems referred to above, the purpose of this utility model is to provide a kind of plant homogenizer.This plant homogenizer has that temperature resistant range is wide, can suddenly cold and hot in safety temperature, the stressed advantage such as strong.
To achieve these goals, the utility model is by the following technical solutions:
One kind of plant homogenizer, comprise base, homogenate tube and homogenate rod, wherein base is provided with cylindrical hole, and described homogenate tube is arranged in the cylindrical hole on base, described homogenate rod is inserted in homogenate tube, by rotating the homogenate rod, the plant cell tissue be positioned in homogenate tube is ground.
The leading section of described homogenate tube is provided with the sample deposition district, radially is provided with the epitaxy part of the lapping liquid overflowed for the splendid attire process of lapping near end.Described sample deposition district is that diameter is the semicircle of 1cm, and described homogenate tube is that diameter is the cylindrical of 1cm, and described epitaxy part is that diameter is the cylindrical of 2cm.
The front end of described homogenate rod is provided with threaded area, and end is provided with handle.The diameter of described homogenate rod is 0.95cm, and described threaded area is arranged at the front end 0.5-3cm place of homogenate rod, the spacing of screw thread be 0.5mm, width be 0.5mm, highly for 0.4mm.
Described base is that the length of side is the square of 6cm, and described cylindrical hole is arranged at the center of base along short transverse.The diameter of described cylindrical hole is that 1.2cm, the degree of depth are 5cm.
Described base, homogenate tube and homogenate rod all adopt stainless steel.Described plant homogenizer needs precooling before using.The precooling temperature of described plant homogenizer is-20 ℃/-70 ℃.
Advantage of the present utility model and beneficial effect are:
1. the utility model can omit the step of liquid nitrogen grinding, and the homogenizer after precooling can reduce the degradation rate of DNA in process of lapping.
2. the Design of Screw Thread of the utility model homogenate nose can increase frictional force, improves grinding rate.
3. base of the present utility model is for firm homogenate tube, and simultaneously, the base of precooling can keep the low temperature environment in homogenate tube.
4. the utility model adopts the homogenizer of stainless steel, it has, and temperature resistant range is wide, can suddenly cold and hot in safety temperature, the stressed advantage such as strong.
5. the utility model has solved frangible, the defect that the grinding dynamics is little of glass homogenizer, has omitted the link of liquid nitrogen simultaneously, and the effect of grinding plant cell tissue is fine.
The accompanying drawing explanation
The structural representation that Fig. 1 is base in the utility model;
The structural representation that Fig. 2 is homogenate tube in the utility model;
The structural representation that Fig. 3 is homogenate rod in the utility model.
Wherein: 1 is base, and 2 is cylindrical hole, and 3 is homogenate tube, and 4 is the sample deposition district, and 5 is the homogenate rod, and 6 is handle, and 7 is threaded area.
Embodiment
Below in conjunction with accompanying drawing, the utility model is further described.
As Figure 1-3, the utility model comprises base 1, homogenate tube 3 and homogenate rod 5, and wherein base 1 is the square of 6cm for the length of side, and material is stainless steel, the center of base 1 is provided with cylindrical hole 2 along short transverse, and the diameter of cylindrical hole 2 is that 1.2cm, the degree of depth are 5cm.Homogenate tube 3 is cylindrical, and adopts stainless steel, and the diameter of homogenate tube 3 is that 1cm, length are 10cm, and the leading section of homogenate tube 3 is provided with sample deposition district 4, and sample deposition district 4 is the semicircle of 1cm for diameter.Radially be provided with the epitaxy part of the lapping liquid overflowed for the splendid attire process of lapping near homogenate tube 3 ends, described epitaxy part is that diameter is that 2cm, length are the cylindrical of 3cm, the upper surface of epitaxy part is 2cm to the distance of the terminal part of homogenate tube 3, the hand-held homogenate tube 3 of convenient operation personnel.Homogenate rod 5 adopts stainless steels, and diameter is 0.95cm, and the front end 0.5-3cm place of homogenate rod 5 is provided with threaded area 7, and the spacing of screw thread is that 0.5mm, width are 0.5mm, highly are 0.4mm, the frictional force that threaded area 7 increases in process of lapping, the speed of raising grinding.The end of homogenate rod 5 is provided with handle 6.The front end of homogenate tube 3 is inserted in the cylindrical hole 2 on base 1, for firm homogenate tube 3.The front end of homogenate rod 5 is inserted in homogenate tube 3, by rotating 5 pairs of plant cell tissues that are positioned in homogenate tube 3 of homogenate rod, is ground.
Working process of the present utility model is:
At first, by the precooling 20 minutes in-20 ℃/-70 ℃ refrigerators of homogenate tube 3, homogenate rod 5 and base 1.The homogenate tube 3 freezed is placed in the cylindrical hole 2 on base 3, and base 3 is placed on the levels operation platform, the base 3 of precooling contributes to keep the low temperature environment in homogenate tube.Be cut into segment by being kept at-70 ℃ of plant samples in refrigerator with scissors, put into homogenate tube 3; Add the lapping liquid that is less than 5ml in homogenate tube 3, left-right rotation homogenate rod 5, produce to grind and cut effect, and plant cell tissue is pulverized, and plant nucleic acid dissolves in lapping liquid; The concrete time of homogenate is depending on floristics, and after probably needing time of 1-2 minute, the lapping liquid that will contain the DNA of plants template is poured in the centrifuge tube of 1.5ml, carries out follow-up test.The utility model is a kind of employing stainless steel, and its temperature resistant range is-196 ℃-800 ℃, can suddenly cold and hot in safe warm area, there is mechanical characteristics.
Claims (8)
1. a kind of plant homogenizer, it is characterized in that: comprise base (1), homogenate tube (3) and homogenate rod (5), wherein base (1) is provided with cylindrical hole (2), described homogenate tube (3) is arranged in the cylindrical hole (2) on base (1), and described homogenate rod (5) is inserted in homogenate tube (3).
2. by plant homogenizer claimed in claim 1, it is characterized in that: the leading section of described homogenate tube (3) is provided with sample deposition district (4), radially is provided with the epitaxy part of the lapping liquid overflowed for the splendid attire process of lapping near end.
3. by plant homogenizer claimed in claim 2, it is characterized in that: described sample deposition district (4) is the semicircle of 1cm for diameter, and described homogenate tube (3) is the cylindrical of 1cm for diameter, and described epitaxy part is that diameter is the cylindrical of 2cm.
4. by plant homogenizer claimed in claim 1, it is characterized in that: the front end of described homogenate rod (5) is provided with threaded area (7), and end is provided with handle (6).
5. by plant homogenizer claimed in claim 4, it is characterized in that: the diameter of described homogenate rod (5) is 0.95cm, described threaded area (7) is arranged at the front end 0.5-3cm place of homogenate rod (5), the spacing of screw thread be 0.5mm, width be 0.5mm, highly for 0.4mm.
6. by plant homogenizer claimed in claim 1, it is characterized in that: described base (1) is the square of 6cm for the length of side, and described cylindrical hole (2) is arranged at the center of base (1) along short transverse.
7. by plant homogenizer claimed in claim 6, it is characterized in that: the diameter of described cylindrical hole (2) is that 1.2cm, the degree of depth are 5cm.
8. by plant homogenizer claimed in claim 1, it is characterized in that: described base (1), homogenate tube (3) and homogenate rod (5) all adopt stainless steel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320314748XU CN203320013U (en) | 2013-06-03 | 2013-06-03 | Plant homogenizing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320314748XU CN203320013U (en) | 2013-06-03 | 2013-06-03 | Plant homogenizing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203320013U true CN203320013U (en) | 2013-12-04 |
Family
ID=49659382
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320314748XU Expired - Fee Related CN203320013U (en) | 2013-06-03 | 2013-06-03 | Plant homogenizing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203320013U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498350A (en) * | 2014-12-16 | 2015-04-08 | 何向锋 | Homogenizer for separating subcellular fractions |
| CN105758692A (en) * | 2016-04-29 | 2016-07-13 | 中国科学院西北高原生物研究所 | Macro fungus pulverizing method suitable for laboratory use |
| CN107400655A (en) * | 2016-05-20 | 2017-11-28 | 台湾粒线体应用技术股份有限公司 | From the method for cell extraction born of the same parents' device |
| CN108982189A (en) * | 2018-09-19 | 2018-12-11 | 遵义医学院 | A kind of low-temperature homogenate device |
-
2013
- 2013-06-03 CN CN201320314748XU patent/CN203320013U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104498350A (en) * | 2014-12-16 | 2015-04-08 | 何向锋 | Homogenizer for separating subcellular fractions |
| CN105758692A (en) * | 2016-04-29 | 2016-07-13 | 中国科学院西北高原生物研究所 | Macro fungus pulverizing method suitable for laboratory use |
| CN107400655A (en) * | 2016-05-20 | 2017-11-28 | 台湾粒线体应用技术股份有限公司 | From the method for cell extraction born of the same parents' device |
| CN108982189A (en) * | 2018-09-19 | 2018-12-11 | 遵义医学院 | A kind of low-temperature homogenate device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203320013U (en) | Plant homogenizing device | |
| CN103525997B (en) | High-speed steel cryogenic treatment process | |
| Liu et al. | Experimental study on the unconfined compression of a thawing soil | |
| CN103160497A (en) | Method for rapidly extracting total RNA (ribonucleic acid) from fish | |
| CN204286891U (en) | One grows tobacco hard tissues cryogrinding device | |
| CN204027890U (en) | A kind of sample preparation device for the preparation of indoor three axle soil samples | |
| CN102896033A (en) | Application method of high-flux plant leaf crusher | |
| CN204525365U (en) | A kind of Broken bolt withdrawing device | |
| CN103275866B (en) | Device and method for smashing plant sample | |
| CN203061253U (en) | Grinding device with liquid nitrogen refrigeration function | |
| CN204320427U (en) | The device of a kind of quick tissue abrasion sample | |
| CN203513686U (en) | Plant sample crushing device | |
| CN103196699A (en) | Static cutting table for frozen earth | |
| CN203990858U (en) | Mortar fixator | |
| WO2012162546A3 (en) | Lance device and associated methods for delivering a biological material into a cell | |
| CN203455196U (en) | Ore pulp chute down-flow automatic sampler | |
| Xiao-dong et al. | Creep deformation and heterogeneous characteristics for frozen soils with thermal gradient | |
| CN103487280B (en) | Botany Experiment is with getting leaf blade | |
| CN203782146U (en) | Continuous low-temperature nucleotide RNA (ribonucleic acid) mortar box | |
| CN102896034A (en) | High-flux plant leaf crusher | |
| Schwander et al. | Electromechanical drilling of a 300 m core in a dry hole at Summit, Greenland | |
| CN201945490U (en) | High-carbon steel specimen cooling device | |
| 邓罡华 | FT-IR and ultrafast IR spectroscopic monitoring of potassium thiocyanate aqueous solution with fast freezing | |
| Kaczmarek et al. | Extreme variability of deformation mechanisms at tiny scale in natural peridotite: the effect of phase distribution and mineral reactions | |
| Skemer et al. | Low Temperature Plasticity of Olivine Determined by Nano-indentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131204 Termination date: 20160603 |