CN203224497U - Colloidal gold detection test paper for bacteria - Google Patents
Colloidal gold detection test paper for bacteria Download PDFInfo
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- CN203224497U CN203224497U CN 201320224322 CN201320224322U CN203224497U CN 203224497 U CN203224497 U CN 203224497U CN 201320224322 CN201320224322 CN 201320224322 CN 201320224322 U CN201320224322 U CN 201320224322U CN 203224497 U CN203224497 U CN 203224497U
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- monoclonal antibody
- colloidal gold
- bacterium
- bacteria
- test paper
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Abstract
The utility model discloses colloidal gold detection test paper for bacteria. The colloidal gold detection test paper comprises a polyvinyl chloride (PVC) soleplate and a nitric acid cellulose membrane, wherein the nitric acid cellulose membrane is arranged on the PVC soleplate, and the colloidal gold detection test paper also comprises a sample cushion, a colloidal gold cushion, a detection area, a quality control area and a water absorption cushion, which are sequentially arranged on the nitric acid cellulose membrane. The colloidal gold detection test paper for bacteria is simple to operate, convenient to use and fast in detection, can be used for detecting bacteria such as streptococcus agalactiae, streptococcus iniae, madtoms edward bacteria, aeromonas sobria, flavobacterium columnare and vibrio fischeri, and has good economical benefit.
Description
Technical field
The utility model relates to a kind of colloidal gold test, particularly the colloidal gold test of a kind of bacterium.
Background technology
All the year round, China aquaculture production operator is many to be target to pursue production capacity with recent economy, and cultivation density is too high, adds that protection breeding environment consciousness is very thin, cultures disease and is the gesture that increases the weight of year by year.According to statistics, nearly 126 kinds of the disease species that China's culture fishery took place, wherein the bacterial disease most species is 61 kinds, account for total disease number near half.
In these bacterial diseases, Streptococcusagalactiae and Streptococcus iniae cause that the streptococcosis Channel-catfish tarda of Tilapia mossambica causes the septicemia of catfish, the fish fin rot that the column Flavobacterium causes and cylindricality disease, the putrid skin disease that Aeromonas sobria causes and tail, and the bacterial vibriosis of vibrio, always be the serious fish disease of puzzlement culture fishery, particularly in recent years in Guangdong, the streptococcosis of Tilapia mossambica main producing region large-scale outbreak such as Hainan, Fujian, the incidence of disease has brought enormous economic loss near 40% to the raiser.
For the check of above-mentioned bacterium, main method has conventional bacterium to separate identification method and PCR method etc. at present, and the sense cycle that bacterium separates identification method is long, does not reach the requirement to the sample fast detecting; Target is subjected to the restriction of factors such as special instrument and equipment and cost costliness in the genomic PCR detection method of thalline, is unfavorable for promoting the use of in the basic unit cultivation base.
The utility model content
In order to address the above problem, the utility model provides the colloidal gold test of a kind of bacterium.
The colloidal gold test of the utility model bacterium, it comprises PVC base plate and nitrocellulose filter, nitrocellulose filter is arranged on the PVC base plate, also comprises the sample pad, collaurum pad, detection zone, Quality Control district and the adsorptive pads that are successively set on the nitrocellulose filter.
Wherein, described collaurum pad is provided with the bacterium monoclonal antibody layer of one deck colloid gold label.
Wherein, the bacterium monoclonal antibody layer of described colloid gold label is that Streptococcusagalactiae monoclonal antibody, Streptococcus iniae monoclonal antibody, Channel-catfish tarda monoclonal antibody, Aeromonas sobria monoclonal antibody, column Flavobacterium monoclonal antibody or vibrio bacterium Monoclonal Antibody by colloid gold label forms.
Wherein, described detection zone is provided with one deck bacterium monoclonal antibody layer.
Wherein, described bacterium monoclonal antibody layer is to be formed by Streptococcusagalactiae monoclonal antibody, Streptococcus iniae monoclonal antibody, Channel-catfish tarda monoclonal antibody, Aeromonas sobria monoclonal antibody, column Flavobacterium monoclonal antibody or vibrio bacterium Monoclonal Antibody.
Wherein, described Quality Control district is provided with the polyclonal antibody floor of the anti-mouse of one deck rabbit.
It is simple to operate, easy to use that the utility model detects test paper, detects fast, with low cost, overcome the defective of existing detection method.
Description of drawings
The colloidal gold test structural representation of Fig. 1 the utility model bacterium.
The colloidal gold test vertical view of Fig. 2 bacterium shown in Figure 1.
Wherein, 1 is the PVC base plate, and 2 is nitrocellulose filter, and 3 is sample pad, 4 is the collaurum pad, and 5 is adsorptive pads, and 6 is detection zone, and 7 are the Quality Control district, 8 is the bacterium monoclonal antibody layer of colloid gold label, and 9 is bacterium monoclonal antibody layer, and 10 is the polyclonal antibody layer of the anti-mouse of rabbit.
Embodiment
As shown in Figure 1, it comprises PVC base plate 1 and nitrocellulose filter 2 colloidal gold test of the utility model bacterium, nitrocellulose filter 2 is arranged on the PVC base plate 1, also comprises the sample pad 3, collaurum pad 4, detection zone 6, Quality Control district 7 and the adsorptive pads 5 that are successively set on the nitrocellulose filter 2.
Wherein, described collaurum pad 4 is provided with the bacterium monoclonal antibody layer 8 of one deck colloid gold label.
Wherein, the bacterium monoclonal antibody layer 8 of described colloid gold label is that Streptococcusagalactiae monoclonal antibody, Streptococcus iniae monoclonal antibody, Channel-catfish tarda monoclonal antibody, Aeromonas sobria monoclonal antibody, column Flavobacterium monoclonal antibody or vibrio bacterium Monoclonal Antibody by colloid gold label forms.
Wherein, described detection zone 6 is provided with one deck bacterium monoclonal antibody layer 9.
Wherein, described bacterium monoclonal antibody layer 9 is to be formed by Streptococcusagalactiae monoclonal antibody, Streptococcus iniae monoclonal antibody, Channel-catfish tarda monoclonal antibody, Aeromonas sobria monoclonal antibody, column Flavobacterium monoclonal antibody or vibrio bacterium Monoclonal Antibody.
Wherein, described Quality Control district 7 is provided with the polyclonal antibody floor 10 of the anti-mouse of one deck rabbit.
Detect sample to be checked whether during bacterial infection with the utility model, the antibody layer that the bacterium monoclonal antibody layer 8 of colloid gold label and bacterium monoclonal antibody layer 9 should adopt the Antibody Preparation of bacterium to be checked to form.Such as, when detected object was Streptococcusagalactiae, the bacterium monoclonal antibody layer 8 of colloid gold label was the Streptococcusagalactiae monoclonal antibody layer of colloid gold label, bacterium monoclonal antibody layer 9 is Streptococcusagalactiae monoclonal antibody layer.
During detection, soak into sample solution to be checked with sample pad, stoichiometric number minute, if red stripes all occurs on the detection zone of test paper and the Quality Control zone position, then sample to be checked is positive for detecting; If only red stripes occurs in the Quality Control zone position, and redfree band on the detection zone position, then sample to be checked is negative for detecting; If red stripes do not occur on the Quality Control zone position of test strips, no matter on the detection line position whether red stripes appears, and testing result is all invalid.
The colloidal gold test of the utility model bacterium detects fast, and simple to operate, with low cost, application prospect is good.
Claims (6)
1. the colloidal gold test of a bacterium, it is characterized in that: it comprises PVC base plate (1) and nitrocellulose filter (2), nitrocellulose filter (2) is arranged on the PVC base plate (1), also comprises the sample pad (3), collaurum pad (4), detection zone (6), Quality Control district (7) and the adsorptive pads (5) that are successively set on the nitrocellulose filter (2).
2. colloidal gold test according to claim 1, it is characterized in that: described collaurum pad (4) is provided with the bacterium monoclonal antibody layer (8) of one deck colloid gold label.
3. colloidal gold test according to claim 2, it is characterized in that: the bacterium monoclonal antibody layer (8) of described colloid gold label is to be formed by the Streptococcusagalactiae monoclonal antibody of colloid gold label, Streptococcus iniae monoclonal antibody, Channel-catfish tarda monoclonal antibody, Aeromonas sobria monoclonal antibody, column Flavobacterium monoclonal antibody or vibrio bacterium Monoclonal Antibody.
4. colloidal gold test according to claim 1, it is characterized in that: described detection zone (6) is provided with one deck bacterium monoclonal antibody layer (9).
5. colloidal gold test according to claim 4, it is characterized in that: described bacterium monoclonal antibody layer (9) is to be formed by Streptococcusagalactiae monoclonal antibody, Streptococcus iniae monoclonal antibody, Channel-catfish tarda monoclonal antibody, Aeromonas sobria monoclonal antibody, column Flavobacterium monoclonal antibody or vibrio bacterium Monoclonal Antibody.
6. colloidal gold test according to claim 1, it is characterized in that: described Quality Control district (7) is provided with the polyclonal antibody floor (10) of the anti-mouse of one deck rabbit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320224322 CN203224497U (en) | 2013-04-27 | 2013-04-27 | Colloidal gold detection test paper for bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320224322 CN203224497U (en) | 2013-04-27 | 2013-04-27 | Colloidal gold detection test paper for bacteria |
Publications (1)
| Publication Number | Publication Date |
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| CN203224497U true CN203224497U (en) | 2013-10-02 |
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| CN 201320224322 Expired - Lifetime CN203224497U (en) | 2013-04-27 | 2013-04-27 | Colloidal gold detection test paper for bacteria |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103901204A (en) * | 2014-04-11 | 2014-07-02 | 深圳市儿童医院 | Immunoturbidimetry kit for detecting streptococcus agalactiae and detection method of immunoturbidimetry kit |
| CN105334324A (en) * | 2015-11-06 | 2016-02-17 | 通威股份有限公司 | Streptococcus agalactiae monoclonal antibody and preparation method and application thereof |
| CN105372422A (en) * | 2015-11-06 | 2016-03-02 | 通威股份有限公司 | Colloidal-gold rapid detection test paper for streptococcus agalactiae |
| CN106442997A (en) * | 2015-12-14 | 2017-02-22 | 广西大学 | Double-antibody sandwich enzyme-linked immunosorbent assay kit for detection of Streptococcus agalactiae |
| CN107462717A (en) * | 2017-08-06 | 2017-12-12 | 潘荣兰 | A kind of fresh water fish bacterial disease pathogen quick diagnosis reagent kit |
| CN107942060A (en) * | 2017-11-16 | 2018-04-20 | 广西大学 | A kind of method using immune colloid gold percolation detection Tilapia mossambica Streptococcusagalactiae antibody |
-
2013
- 2013-04-27 CN CN 201320224322 patent/CN203224497U/en not_active Expired - Lifetime
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103901204A (en) * | 2014-04-11 | 2014-07-02 | 深圳市儿童医院 | Immunoturbidimetry kit for detecting streptococcus agalactiae and detection method of immunoturbidimetry kit |
| CN103901204B (en) * | 2014-04-11 | 2016-01-27 | 深圳市儿童医院 | A kind of immunoturbidimetry kit and detection method thereof detecting Streptococcusagalactiae |
| CN105334324A (en) * | 2015-11-06 | 2016-02-17 | 通威股份有限公司 | Streptococcus agalactiae monoclonal antibody and preparation method and application thereof |
| CN105372422A (en) * | 2015-11-06 | 2016-03-02 | 通威股份有限公司 | Colloidal-gold rapid detection test paper for streptococcus agalactiae |
| WO2017075937A1 (en) * | 2015-11-06 | 2017-05-11 | 通威股份有限公司 | Colloidal-gold rapid detection test paper for streptococcus agalactiae |
| CN105334324B (en) * | 2015-11-06 | 2017-06-27 | 通威股份有限公司 | Streptococcusagalactiae monoclonal antibody and its production and use |
| CN106442997A (en) * | 2015-12-14 | 2017-02-22 | 广西大学 | Double-antibody sandwich enzyme-linked immunosorbent assay kit for detection of Streptococcus agalactiae |
| CN107462717A (en) * | 2017-08-06 | 2017-12-12 | 潘荣兰 | A kind of fresh water fish bacterial disease pathogen quick diagnosis reagent kit |
| CN107942060A (en) * | 2017-11-16 | 2018-04-20 | 广西大学 | A kind of method using immune colloid gold percolation detection Tilapia mossambica Streptococcusagalactiae antibody |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20131002 |