CN2031929U - Electrophoretic tank capable of generating equal-strength alternating current field - Google Patents

Abstract

Disclosed is an electrophoretic tank capable of generating equal-strength alternating current field, provides an electrophoretic tank capable of generating alternating current field applying to the work of biochemistry electrophoretic analysis, particularly the work of electrophoretic analysis to long chain macromolecules like DNA, and is composed of a buffer solution tank and a plurality of small mutually independent electrodes. The utility model can generate approximate equal-strength altering current field so as to make the experiment condition of each sample identical, and make the strips of electropherogram parallel; the analysis of the electropherogram by using densitometer is convenient.

Description

Electrophoretic tank capable of generating equal-strength alternating current field

The utility model is a kind of electrophoresis tank that produces even strong exchange current field, is the instrument that is used for the biological chemistry electrophoretic analysis.

Electrophoresis is a kind of of electrokinetic phenomena, is meant electrically charged particle in dispersed system, the phenomenon of moving towards respective electrode under the electric field force effect.Biological chemistry electrophoretic analysis technology is widely used in fundamental research, clinical examination and agriculture, forestry, animal husbandry and fishery production.The utensil that electrophoresis carries out therein is called electrophoresis tank.The current field at sample to be analyzed place should be even strong in the electrophoresis tank, and each sample to be analyzed is compared under identical experiment condition.Non-even electricity field flow field also can make each band in the electrophoresis pattern not parallel, is difficult to come electrophoresis pattern is analyzed with the autoscan opacimeter.

If the current field direction at sample to be analyzed place is unfixing, but alternately changes, then can greatly improve DNA (deoxyribonucleic acid) to DNA(on two different directions) the macromolecular resolution characteristic of this class long-chain shape.Existing exchange current field electrophoresis tank, for example the Sweden LKB company Pulsephor(trade name of producing), be that electrode is housed is square flat horizontal strip electrophoresis groove on four limits.Fig. 1 is the schematic top plan view of existing this class exchange current field electrophoresis tank.Among Fig. 1, four limits of buffer pool (2) are designated as east, south, west, the north respectively, respectively put an electrode (1) on west, the north, respectively put three electrodes (1) on east, south.Each electrode is all very little, all can move along the limit, and each electrode is with a crystal diode and other electrode isolation.Circuit during electrophoresis such as Fig. 2, each limit electrode of existing exchange current field electrophoresis tank (3) connects with the corresponding output terminal of divider (4), and the input end of divider (4) connects with the positive-negative output end of electrophoresis apparatus (5).During electrophoresis, add a certain amount of damping fluid in the buffer pool, form a foursquare uniform liquid thin layer, the north and south of electrophoresis tank (3) and thing two arrays of electrodes wheel flow-thru electrode (energising is meant that the DC voltage that electrophoresis apparatus (5) provides puts on the electrode on this limit via divider (4)), that is: period 1 north and south group energising (negative north, south just), thing group suspension (east, electrode on arbitrary limit, west and south, electrode on the north does not all have the connection of the electricity outside the electrophoresis tank), in damping fluid, produce the current field that direction is roughly to the south thus; East, west group energising second round (negative west, east is just), south, north group suspension, direction of generation eastwards current field roughly in damping fluid; Move in circles like this, all odd cycle all produce the current field that direction is roughly to the south in damping fluid, and all even cycle all produce roughly eastwards current field of a direction.So the current field in the damping fluid is an alternation, its period of change is determined by divider (4).Actual divider has the complicated electronic circuit of a cover to finish regularly with the work of Zhuan Change, is drawn as the switch of a double-pole dibit among Fig. 2, only is its principle of work of explanation.

The current field that the shortcoming of aforementioned existing exchange current field electrophoresis tank is wherein and is produced is very inhomogeneous.This is owing to respectively having only a very little electrode on west, the north.Uneven current field can make the experiment condition of each sample to be analyzed inconsistent as previously mentioned, can not compare accurately, and band is not parallel in the electrophoresis pattern, is difficult to analyze with the autoscan opacimeter.

The purpose of this utility model provides a kind of horizontal strip electrophoresis groove, and its structure makes it can produce approximate even strong exchange current field.

The purpose of this utility model realizes by following structure: a plurality of small electrodes are installed on each limit on square or rectangular horizontal buffer pool four limits, and electrode can be fixed in pool wall, can be isolating construction with buffer pool also.Said small electrode is meant that the electrode dimension is less than 1/5th of interelectrode distance from overlooking direction.Number of electrodes is many more, and current field is even more, so every limit number of electrodes should not be less than 6, but then, when every limit number of electrodes surpassed 40, the increase of number of electrodes was little to the inhomogeneity improvement of current field.Every limit number of poles that powers on should also can all equate about equally.With between each electrode on one side when the no power of this limit mutual insulating, and when the energising of this limit, be linked together into equipotential with mechanical switch or electronic switch.This structure makes arbitrary limit be similar to the boundary condition of the equipotentiality that satisfies the requirement of plane current place, rectangular area owing to number of electrodes when energising, and very little and mutual insulating is similar to the boundary condition that satisfies the insulation that plane current place, rectangular area requires owing to each electrode when no power, thereby the current field that is produced is approximate even strong.And during two groups of opposite side wheel flow-thru electrodes, current field is exactly an alternation and approximate even strong.The degree of irregularity of this current field is relevant with interelectrode distance, concerning electrophoretic analysis, electrophoretic analysis work is not had influence to each back gauge greater than the degree of irregularity of the each point current field of two times of electrode separations in the damping fluid in the electrophoresis tank.

Compared with prior art, the utility model can produce than prior art even many current fields, so can make the experiment condition of each sample to be analyzed identical, and the electrophoresis pattern band is parallel, is easy to optical density and scores and analyse, and has improved the order of accuarcy of electrophoretic analysis.When the structure of the previous embodiment that will describe in detail below taking, constant with the connecting mode of divider, existing divider needn't be changed and can use.

Be further described below with reference to embodiment.

Fig. 3 is the concrete structure (partly cut open and face and vertical view) of a kind of embodiment of the utility model, and the square buffer pool (6) that the length of side that this electrophoresis tank is made of plastics is 150 millimeters to 500 millimeters, four screws (8) of regulating buffer pool (6) one-tenth level and 15 on every limit amount to 60 electrodes (7) and form.A foursquare recess (9) was arranged on the end of buffer pool (6), this recess (9) is used to put into the glass plate of carrying gel, the degree of depth of recess (9) sheet thickness therewith equates, become a plane at the bottom of making glass plate put into the back damping fluid, the damping fluid degree of depth is identical everywhere, in order to avoid owing to putting into of glass plate caused the different homogeneitys that influence current field of the damping fluid degree of depth everywhere.Fig. 4 is the structure (just analysing and observe and left and right view) of an electrode, electrode (7) is made by the platinum filament of the about 0.3mm of diameter, platinum filament from inside to outside passes two apertures in the same vertical plane on buffer pool (6) wall, and is stranded pool wall outside, and the stranded part while is also as the exit of electrode.Platinum filament stranded fixing after, with rubber-like or polyurethanes sealed binder the one-component silicone rubber bonding agent of handy cold curing () inject aperture with the hole sealing in case damping fluid spill by aperture.Said two apertures, the aperture of below should be higher than at the bottom of the pond 1 millimeter, and the aperture of top should be higher than the buffering liquid level.Each electrode and a crystal diode link, and as shown in Figure 5, each electrode links with the corresponding diode cathode on this limit on west, the north, and each electrode links with the corresponding diode cathode on this limit on east, the south.Each diode is not linked together with the joining end of electrode on arbitrary limit, links with the corresponding lead-out terminal of divider (4) again.Because conducting when all diodes are switched on this limit on arbitrary limit, its voltage drop is very little, so the electromotive force of each electrode equates on this limit; And arbitrary limit is connected to one positive one anti-two diode in series between any two electrodes on this limit when the no power of this limit, so there is not electric current on each electrode, satisfy the requirement of each electrode mutually insulated.For preventing that the user from getting an electric shock, the cover that all bare metal parts should be made with insulating material after assembling finishes covers; For preventing the damping fluid evaporation, can add a cover on the buffer pool.

Method of operating when the electrophoresis tank of this structure uses and general horizontal strip electrophoresis groove are identical.

Another embodiment, the structure of its buffer pool and electrode and previous embodiment are identical, but do not use electronic switch and use mechanical switch.As shown in Figure 6, each electrode and a switch link, each switch is not connected to the positive output end of electrophoresis apparatus (5) on west, the north with the joining end of electrode, and each switch is not connected to the negative output terminal of electrophoresis apparatus (5) on east, the south with the joining end of electrode.All each switches all link; When each switch became path on south, the north, each switch became to open circuit on east, the west; And south, when each switch becomes to open circuit on the north, each switch becomes path on east, the west.For reducing volume and being convenient to control alternating cycles, all switches all use tongue tube, south, the shared coil drive of each tongue tube on the shared coil of each tongue tube and east, the west on the north, this two coil wheels circulation excess current and when making each tongue tube action, two groups of opposite side of electrophoresis tank are promptly taken turns flow-thru electrode, thereby produce alternation and approximate even strong current field.

Claims (2)

1, a kind of exchange current field electrophoresis tank, constitute by four groups every group a plurality of electrodes on square or rectangle buffer pool and four limits, it is characterized in that: each electrode on the arbitrary limit of buffer pool is mutual insulating when the no power of this limit, and is linked together into equipotential with electronic switch or mechanical switch when the energising of this limit.

2, by the described electrophoresis tank of claim 1, it is characterized in that: each electrode on the arbitrary limit of buffer pool is by connecting mutually or mutual insulating with the joining crystal diode of each electrode.

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