CN203065483U - Equipment for collecting microorganisms from fluid - Google Patents
Equipment for collecting microorganisms from fluid Download PDFInfo
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- CN203065483U CN203065483U CN 201220526504 CN201220526504U CN203065483U CN 203065483 U CN203065483 U CN 203065483U CN 201220526504 CN201220526504 CN 201220526504 CN 201220526504 U CN201220526504 U CN 201220526504U CN 203065483 U CN203065483 U CN 203065483U
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Abstract
The utility model provides equipment for collecting microorganisms from a fluid. The equipment comprises a body, a negative pressure generating component, and a filtering and collecting component, wherein a negative pressure chamber is limited in the body, the negative pressure generating component is arranged inside the negative pressure chamber and the filtering and collecting component is arranged on at least one side of the negative pressure chamber. By adopting the equipment, the microorganisms can be effectively collected from the fluid.
Description
Technical field
The utility model relates to environmental microbiology, and particularly, the utility model relates to the equipment of collecting microorganism in the fluid.
Background technology
Air is the environment that the mankind depend on for existence, also be microorganism diffusion and pathophorous medium, microorganism mainly comprises multiple microorganisms such as bacterium, fungi, virus and actinomycetes in the air, this is the air-polluting chief component, and generation and the mankind's activity of these microorganisms have close relationship.Microorganism mainly derives from natural water body, soil, animals and plants and the mankind in the air, rural activity, animal rearing, industrial production, sewage disposal, fermenting process and foodstuff production factory etc., how much microorganism is one of major criterion of Air quality in the air.Understanding airborne microorganism just must be with microbial enrichment, in order to observe and analyze, this just needs the air microorganism sampler of particular design.
The air microbe sampling method mainly is divided into natural sedimentation and machine sampling method.Natural sedimentation is the action of gravity that utilizes the air microbe particle, in the regular hour, allows microbe granular in the air in zone of living in progressively be deposited to a kind of method of sampling in the plate that has developing medium.Because this method is passive sampling, sampling condition is difficult to control, and the drift of microorganism particle, diffusion and sedimentation meeting are subjected to the interference of various power such as wind-force, electric power, magnetic force, heating power, buoyancy and diffusive force, and therefore this method of sampling can cause very big error.The machine sampling method is to sample with sampling thief, and according to sampling principle, sampling thief can be divided into decanter type, collision type, centrifugal collision type, filtering type, electrostatic, Cyclonic etc.In addition, also have temperature difference forced landing class and biological species.The development of various sampling thiefs and use have promoted the development of aerobiology greatly, are out of this world but still there is not a kind of sampling thief.
The utility model content
The utility model one of is intended to solve the problems of the technologies described above at least to a certain extent or provides a kind of useful commerce to select at least.For this reason, a purpose of the present utility model is to propose a kind of equipment of collection micro-biological samples from fluid effectively.
For this reason, the utility model proposes the equipment of collecting microorganism the fluid from a kind of, comprising: body is limited with negative pressure cavity in the described body; The negative pressure generating assembly, described negative pressure generating assembly is arranged in the described negative pressure cavity; And the filtration collection assembly, described filtration collection assembly is arranged at least one side of described negative pressure cavity.Utilize this equipment, can from fluid, collect microorganism effectively.
Adopted the enrichment mode of non-natural subsidence according to the equipment that is used for collection fluid microorganism of the utility model embodiment, can overcome passive sampling, prevent microorganism drift, spread and be subjected to the interference in various such as wind-force, electric power, magnetic force, heating power, buoyancy and diffusive force.Especially, by adopting active direct suction to filter collection method, can realize whole microorganisms are comprised the disposable synchronous collection of bacterium, fungi, virus etc., this has overcome the defective of the substratum method of sampling of prior art, thereby collects micro-biological samples in the fluid effectively.In the existing substratum method of sampling, since nutritional condition and training method difference, the limitation that can cause air microbe to collect.In addition, can be used for collecting the air sample of various different open environments, not only broad covered area but also easy and simple to handle according to the equipment of the utility model embodiment.The method of the extraction air sample nucleic acid that provides in the utility model in addition, is very effective.
Additional aspect of the present utility model and advantage part in the following description provide, and part will become obviously from the following description, or recognize by practice of the present utility model.
Description of drawings
Above-mentioned and/or additional aspect of the present utility model and advantage are from obviously and easily understanding becoming the description of embodiment in conjunction with following accompanying drawing, wherein:
Fig. 1 has shown the device structure synoptic diagram according to microorganism in the collection fluid of the utility model embodiment, and wherein A, B and C have shown the device structure synoptic diagram according to microorganism in the collection fluid of the utility model embodiment respectively;
Fig. 2 has shown the perspective exploded view according to the filtration collection assembly of the equipment of microorganism in the collection fluid of the utility model embodiment;
Fig. 3 has shown the figure as a result with 2100 chip detection of extracting behind the RNA according to the utility model embodiment from air sample; And
Fig. 4 has shown that the N,O-Diacetylmuramidase that only adopts according to the utility model embodiment extracts the back figure as a result with 2100 chip detection of air sample 1RNA extraction.
Embodiment
Describe embodiment of the present utility model below in detail, the example of described embodiment is shown in the drawings, and wherein identical or similar label is represented identical or similar elements or the element with identical or similar functions from start to finish.Be exemplary below by the embodiment that is described with reference to the drawings, be intended to for explaining the utility model, and can not be interpreted as restriction of the present utility model.
In first aspect of the present utility model, the utility model proposes a kind of for the equipment of collecting microorganism from fluid especially air.According to embodiment of the present utility model, with reference to Figure 1A～C, the embodiment of these equipment 1000 utility models, the equipment 1000 of collecting microorganism in the fluid comprises: body 100, filtration collection assembly 200 and negative pressure generating assembly 300.
According to embodiment of the present utility model, be limited with negative pressure cavity 110 in the body 100, negative pressure generating assembly 300 is arranged in the negative pressure cavity 110, be used in negative pressure cavity 110, forming negative pressure, filter collection assembly 200 and be arranged at least one side of negative pressure cavity 110, in order to make fluid to enter negative pressure cavity 110 by filtering collection assembly 200.
Adopted the enrichment mode of non-natural subsidence according to the equipment that is used for collection fluid microorganism of the utility model embodiment, can overcome passive sampling, prevent microorganism drift, spread and be subjected to the interference in various such as wind-force, electric power, magnetic force, heating power, buoyancy and diffusive force.Especially, by adopting active direct suction to filter collection method, can realize whole microorganisms are comprised the disposable synchronous collection of bacterium, fungi, virus etc., this has overcome the defective of the substratum method of sampling of prior art, thereby collects micro-biological samples in the fluid effectively.In the existing substratum method of sampling, since nutritional condition and training method difference, the limitation that can cause air microbe to collect.
Need to prove, though given equipment is vertical in the accompanying drawings, it will be appreciated by persons skilled in the art that also and can adopt other forms, for example horizontal.
According to embodiment of the present utility model, the type of negative pressure generating assembly 300 also is not particularly limited, and for example can be vacuum extractor.In addition, with reference to Figure 1B and C, negative pressure generating assembly 300 further comprises blade 320; Drive unit 310, described drive unit 320 links to each other by union lever 330 with described blade 310, is used for driving described blades turning.Thereby by the rotation of blade 310, drive flowing of negative pressure cavity 110 interior air, can in negative pressure cavity, form local decompression, thereby actuating fluid enters negative pressure cavity 110 by filtering collection assembly 200.According to example of the present utility model, be formed with through hole 120 at the sidewall relative with described filtration collection assembly.Thus, can in the rotation process of blade 310, the gas in the negative pressure cavity 110 be discharged negative pressure cavity 110, thereby can in negative pressure cavity 110, form continual and steady negative pressure, thereby improve the especially efficient of micro-biological samples of collection of biological sample effectively.
According to examples more of the present utility model, described blade 310 is oppositely arranged with filtering collection assembly 200, and the filtration collection assembly removably links to each other with described housing.Thus, can further drive air flowing by the blade rotation effectively, and from through hole 120 air be discharged, thereby form negative pressure for 110 kinds at negative pressure cavity, form negative pressure simply and easily.
According to embodiment of the present utility model, the equipment 1000 of collecting microorganism in the fluid further comprises: limiting component 400, described limiting component 400 are arranged on the described body 110, are used for carrying out spacing to described filtration collection assembly 200.According to examples more of the present utility model, limiting component 400 is arranged on the described body 110 movably.Thus, can the position of filtering collection assembly 200 be limited.
, filter collection assembly 200 and comprise: filter membrane 22 according to example of the present utility model with reference to figure 2; Collecting net cell 21 before the film; With net formula liner 23 behind the film, wherein, described filter membrane 22 is arranged on before the described film behind the collecting net cell 21 and described film between the net formula liner 23.According to embodiment of the present utility model, the pore size of filter membrane 22 also is not particularly limited, and according to embodiment of the present utility model, can be 0.1～10 micron.The kind of described filter membrane also is not particularly limited, according to embodiment of the present utility model, can for be selected from nylon membrane filter membrane, polyester nucleopore membranes, polypropylene screen, nylon membrane, PVDF membrane and cellulose acetate film one of at least.According to an example of the present utility model, described filter membrane is preferably cellulose acetate film.
According to embodiment of the present utility model, filtering collection assembly 200 further comprises: seal frame 24, described seal frame 24 is arranged at before the film behind the collecting net cell 21 and film between the net formula liner 23, is used for net formula liner 22 behind collecting net cell 24 and the film is sealed.According to embodiment of the present utility model, the material of sealing framework 24 also is not particularly limited, and according to example of the present utility model, described framework can be the rubber frame frame.Thus, can further guarantee before the film being tightly connected of net formula liner 22 behind the collecting net cell 24 and film effectively.
Understand for convenience, below to extracting the method for nucleic acid from biological specimen, the especially suitable biological specimen that extracts from the equipment that the utilizes the utility model embodiment especially method of micro-biological samples extraction nucleic acid is described.
In second aspect of the present utility model, the utility model provides a kind of method of extracting nucleic acid from biological specimen.According to embodiment of the present utility model, the method for this extraction nucleic acid may further comprise the steps: the described biological specimen of cracking, in order to obtain containing the split product of nucleic acid; And from described split product, separate described nucleic acid.According to embodiment of the present utility model, the described biological specimen of cracking further comprises: utilize the mixture that contains N,O-Diacetylmuramidase, helicase and lywallzyme, described biological specimen is digested; Digestion product is centrifugal, collecting precipitation; And utilize lysate, described precipitation is carried out cracking, in order to obtain the described split product that contains nucleic acid.According to an example of the present utility model, preferably, the ratio of described N,O-Diacetylmuramidase, helicase and lywallzyme is 1:1:1.The contriver is surprised to find, by adopting the combination of N,O-Diacetylmuramidase, helicase and lywallzyme, can digest biological specimen effectively, thus can be being significantly higher than the efficient of prior art, especially from air, extract nucleic acid in the collected microorganism from biological specimen.For this reason, the method from biological specimen extraction nucleic acid according to the utility model embodiment, can from biological specimen, extract nucleic acid effectively, especially the effect to the microorganism broken wall is obvious, can extract DNA and RNA simultaneously, particularly the integrity of RNA and purity are all very high, and simultaneously this method also is applicable to for example extraction of the nucleic acid of microorganism in the air of various environmental samples.
According to embodiment of the present utility model, can be used for the type of lysate of the present utility model and be not particularly limited according to embodiment more of the present utility model, described lysate can contain 200mmol/LNaCl, 100mmol/L Tris-HCl, pH8.0,2.0%SDS, 50mmol/L EDTA, 0.5%Triton X-100.According to an example of the present utility model, can further contain Proteinase K in the described lysate.According to embodiment more of the present utility model, described cracking is carried out under 50-55 degree centigrade.Thus, can further improve the cracking biological sample especially from the efficient of microbiological specimens, thereby further improve the efficient of extracting nucleic acid from biological sample.
According to embodiment of the present utility model, isolating nucleic acid further comprises from this split product: described split product is carried out centrifugal, collect first supernatant liquor; The mixture that will contain phenol, chloroform and primary isoamyl alcohol mixes according to equal-volume with described first supernatant liquor, carry out centrifugal after, collect second supernatant liquor, wherein, the volume ratio of phenol, chloroform and primary isoamyl alcohol is 25:24:1 in the described mixture that contains phenol, chloroform and primary isoamyl alcohol; The mixture that will contain chloroform and primary isoamyl alcohol mixes according to equal-volume with described second supernatant liquor, carry out centrifugal after, collect the 3rd supernatant liquor; And the mixture that will contain Virahol and sodium-acetate mixes according to the volume ratio of 0.7:1 with described the 3rd supernatant liquor, carry out centrifugal after, collect the nucleic acid precipitation.Thus, can further improve the purity of resultant nucleic acid samples significantly.
According to embodiment of the present utility model, can utilize method of the present utility model to extract the sample source of nucleic acid and be not particularly limited.According to embodiment more of the present utility model, the biological specimen that adopts is the micro-biological samples from fluid collection.According to embodiment of the present utility model, can be by following method from the fluid collection micro-biological samples:
Under action of pressure, make the fluid that contains microorganism by filter membrane, wherein, the pressure of described filter membrane sample introduction side is higher than the pressure that described filter membrane goes out the sample side, and the aperture of described filter membrane makes described microorganism be trapped within on the described filter membrane.Thus, can from fluid, collect micro-biological samples effectively, thereby further improve the efficient of extracting nucleic acid from biological sample.According to embodiment of the present utility model, can utilize the type of the micro-biological samples that method of the present utility model handles and be not particularly limited, according to examples more of the present utility model, described microorganism can for be selected from bacterium, fungi, virus and actinomycetic one of at least.
According to embodiment of the present utility model, the fluid type that can adopt also is not particularly limited, and according to specific embodiment of the utility model, the fluid that can adopt is gas, for example can be air.Thus, utilize according to embodiment of the present utility model, can for example collect microbiological specimens the air from gas effectively
According to embodiment of the present utility model, pressure difference is to go out the directed flow that the sample side forms air and form by described, and its optimal pressure range is to be higher than 1000Pa, and is no more than 3000Pa.The contriver finds, when pressure difference is no more than 1000Pa, fluid especially air will be difficult to pass through effectively filter membrane, from fluid especially air the collection of biological sample especially the efficient of microorganism will significantly reduce, and when pressure surpasses 3000Pa, biological specimen especially micro-biological samples will be difficult to rest on the filter membrane, thus the collection of biological sample especially the efficient of microorganism also will significantly reduce.
According to an example of the present utility model, the pore size of described filter membrane also is not particularly limited, and can be 0.1～10 micron.According to example of the present utility model, the kind of described filter membrane also is not particularly limited, can for be selected from nylon membrane filter membrane, polyester nucleopore membranes, polypropylene screen, nylon membrane, PVDF membrane and cellulose acetate film one of at least.According to embodiment of the present utility model, preferred cellulose acetate film because cellulose acetate film has very high flow velocity and thermostability and low-down absorption, is fit to Sterile Filtration very much.According to embodiment of the present utility model, the aperture of described cellulose acetate film can be 0.22 micron.
Below in conjunction with embodiment scheme of the present utility model is made an explanation.It will be understood to those of skill in the art that the following examples only are used for explanation the utility model, and should not be considered as limiting scope of the present utility model.Unreceipted concrete technology or condition among the embodiment, according to the described technology of the document in this area or condition (for example with reference to works such as J. Sa nurse Brookers, " the molecular cloning experiment guide " that Huang Peitang etc. translate, the third edition, Science Press) or carry out according to product description.The unreceipted person of production firm of agents useful for same or instrument is and can for example can purchases from Illumina company by the conventional products of commercial acquisition.
Embodiment 1
1, prepares before the sample collection
According to Fig. 1 C and structure shown in Figure 2, get a new cellulose acetate membrane, will filter collection assembly and be connected with housing, be ready to collect equipment.
2, sample collection
With air enriching apparatus switch opens, under 1000Pa＜H≤3000Pa pressure, in the workshop of two distillerys, start shooting continuously respectively and bled 3 days, bleed after the end, take out filter membrane in the grid groove, in super clean bench, the filter membrane of tearing is collected thalline, collect air sample 1 and air sample 2.
3, air sample nucleic acid extraction
The thalline of collecting is placed the 50mL centrifuge tube with the top layer filter membrane that tears it down, to wherein adding 20mL TE, 200 μ L10mg/mL N,O-Diacetylmuramidases, 200 μ L10mg/mL helicases and 200 μ L10mg/mL lywallzymes, the pressure-vaccum mixing, 37 ℃ are incubated overnight, and mixing instrument mixing (digested overnight) is used in digestion simultaneously; With gained Digestive system centrifugal 10min under 4 ℃ of 9000r/min, abandoning supernatant; In precipitation, add 6mL TENS lysate (200mmol/L NaCl, 100mmol/L Tris-HCl, pH8.0,2.0%SDS, 50mmol/L EDTA, 0.5%Triton X-100) and 20mg/mL Proteinase K 300 μ L, put upside down mixing, 55 ℃ of temperature are bathed 1h, put upside down mixing once every 15min; With the centrifugal 10min under 4 ℃ of 10000r/min conditions of sample after the cracking, leave and take supernatant liquor, discard precipitation; Add isopyknic phenol in the gained supernatant liquor: chloroform: primary isoamyl alcohol (25:24:1), fully mixing, centrifugal 10min under 4 ℃ of 12000r/min then; Shift the gained supernatant liquor to new pipe, to wherein adding isopyknic chloroform: primary isoamyl alcohol (24:1), abundant mixing, centrifugal 10min under 4 ℃ of 12000r/min; The gained supernatant liquor is changed in the 1.5mL centrifuge tube, to wherein adding 0.7 times of volume Virahol and 100 μ L5M sodium-acetates, behind the precipitation 2h, centrifugal 30min under 4 ℃ of 13000rpm conditions; Discard the gained supernatant liquor, ice-cold 75% ethanol rinsing precipitation secondary, centrifugal 5min under 4 ℃ of 13000rpm conditions; Abandoning supernatant, air-dry, precipitation is with 30 μ L sterilized waters dissolvings, and as if only extracting RNA, the DNA enzyme I that can add 1 μ L does not have a RNA enzyme comes dissolution precipitation; Adopt Nanodrop instrument and 2100 chips to detect DNA and RNA concentration and the purity of the nucleic acid that extracts respectively then.
4, air sample extracts the result
Fig. 3 has shown from air sample the figure as a result that extracts behind the RNA with 2100 chip detection.Fig. 3 shows:
The whole result of sample 2:
RNA area: 169.7
RNA concentration: 98ng/ microlitre
τ RNA ratio (23s/16s): 1.0
RNA integrity counting (RIN): 7.6 (B.02.07)
The sheet segment table of sample 2:
In addition, repeat the above-mentioned method of from air, extracting microbial nucleic acids, just when extracting sample of nucleic acid, only adopt N,O-Diacetylmuramidase.Fig. 4 has shown that only adopting N,O-Diacetylmuramidase to extract air sample 1RNA extracts back figure as a result with 2100 chip detection.
Fig. 4 shows:
The whole result of sample 6:
RNA area: 78.1
RNA concentration: 58ng/ microlitre
τ RNA ratio (28s/18s): 0
RNA integrity counting (RIN): 2.5 (B.02.07)
The sheet segment table of sample 2:
The initial size of title (n ι) stops the per-cent of size (n ι) area total area
18s 1，696 1，891 0.4 0.5
By the result of Fig. 3 and Fig. 4 as can be known, utilize apparatus and method of the present utility model, enriched microorganism from air can also extract sample of nucleic acid from the microorganism of institute's enrichment simultaneously effectively effectively.In addition, effective broken not as three kinds of enzymes (N,O-Diacetylmuramidase+helicase+wall breaking enzyme) work of the effect that only adopts N,O-Diacetylmuramidase disruption of microorganisms sample, helicase and lywallzyme will be got well the effect of fungi and gram-positive microorganism broken wall, and three kinds of enzymes one work, can strengthen the effect of enzyme mutually, embody synergistic action effect, make enzyme performance best effect.
Embodiment 2
Place the 2mL centrifuge tube to add 500mL TE and 20 μ L10mg/mL N,O-Diacetylmuramidases and 20 μ L10mg/mL helicases, 20 μ L10mg/mL lywallzymes the bovine rumen sample 0.5g that collects, the pressure-vaccum mixing, 37 ℃ are incubated overnight, and mixing instrument mixing (digested overnight) is used in digestion simultaneously; With Digestive system centrifugal 10min under 4 ℃ of 9000r/min conditions of gained, remove supernatant liquor, leave and take precipitation; In precipitation, add 400 μ LTENS lysate (200mmol/L NaCl, 100mmol/L Tris-HCl, pH8..0,2.0%SDS, 50mmol/L EDTA, 0.5%Triton X-100) and 20mg/mL Proteinase K 300 μ L, put upside down mixing, 55 ℃ of temperature are bathed 1h, put upside down mixing once every 15min; With the centrifugal 10min under 4 ℃ of 10000r/min conditions of the sample after the cracking, leave and take supernatant, discard precipitation; Add isopyknic phenol in the supernatant liquor: chloroform: primary isoamyl alcohol (25:24:1), abundant mixing, and under 4 ℃ of 12000r/min conditions centrifugal 10min; Shift the gained supernatant liquor to new pipe, to wherein adding isopyknic chloroform: primary isoamyl alcohol (24:1), abundant mixing, centrifugal 10min under 4 ℃ of 12000r/min; The gained supernatant liquor is changed in the 1.5mL centrifuge tube, to wherein adding 0.7 times of volume Virahol and 10 μ L5M sodium-acetates, behind the precipitation 2h, centrifugal 30min under 4 ℃ of 13000rpm conditions; Discard the gained supernatant liquor, ice-cold 75% ethanol rinsing precipitation secondary, centrifugal 5min under 4 ℃ of 13000rpm conditions; Abandoning supernatant, air-dry, precipitation adds 1 μ L RNaseA with the dissolving of 30 μ L sterilized waters in the dissolution process, obtain the DNA of bovine rumen sample.
Embodiment 3
Adopt the plaque sample, repeat embodiment 2 and extract DNA.
In the description of this specification sheets, concrete feature, structure, material or characteristics that the description of reference term " embodiment ", " some embodiment ", " example ", " concrete example " or " some examples " etc. means in conjunction with this embodiment or example description are contained at least one embodiment of the present utility model or the example.In this manual, the schematic statement to above-mentioned term not necessarily refers to identical embodiment or example.And concrete feature, structure, material or the characteristics of description can be with the suitable manner combination in any one or more embodiment or example.
Although illustrated and described embodiment of the present utility model above, be understandable that, above-described embodiment is exemplary, can not be interpreted as restriction of the present utility model, those of ordinary skill in the art in scope of the present utility model, can change above-described embodiment under the situation that does not break away from principle of the present utility model and aim, modification, replacement and modification.
Claims (10)
1. an equipment of collecting microorganism in the fluid is characterized in that, comprising:
Body is limited with negative pressure cavity in the described body;
The negative pressure generating assembly, described negative pressure generating assembly is arranged in the described negative pressure cavity; And
Filter collection assembly, described filtration collection assembly is arranged at least one side of described negative pressure cavity.
2. the equipment of microorganism in the collection fluid according to claim 1 is characterized in that described negative pressure generating assembly comprises:
Blade;
Drive unit, described drive unit links to each other with described blade, is used for driving described blades turning.
3. the equipment of microorganism in the collection fluid according to claim 2 is characterized in that, is formed with through hole at the sidewall relative with described filtration collection assembly.
4. the equipment of microorganism in the collection fluid according to claim 2 is characterized in that, described blade and described filtration collection assembly are oppositely arranged.
5. the equipment of microorganism in the collection fluid according to claim 1 is characterized in that described filtration collection assembly removably links to each other with described housing.
6. the equipment of microorganism in the collection fluid according to claim 1 is characterized in that, further comprises:
Limiting component, described limiting component is arranged on the described body movably.
7. the equipment of microorganism in the collection fluid according to claim 6 is characterized in that described filtration collection assembly comprises:
Filter membrane;
Collecting net cell before the film; With
Net formula liner behind the film,
Wherein, described filter membrane is arranged on before the described film behind the collecting net cell and described film between the net formula liner.
8. the equipment of microorganism in the collection fluid according to claim 7, it is characterized in that, described filter membrane be selected from nylon membrane filter membrane, polyester nucleopore membranes, polypropylene screen, nylon membrane, PVDF membrane and cellulose acetate film one of at least, the aperture of described filter membrane is 0.1～10 micron.
9. the equipment of microorganism in the collection fluid according to claim 7 is characterized in that described filtration collection assembly further comprises:
Seal frame is arranged at before the described film behind the collecting net cell and described film between the net formula liner.
10. the equipment of microorganism in the collection fluid according to claim 9 is characterized in that described framework is the rubber framework.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220526504 CN203065483U (en) | 2012-10-15 | 2012-10-15 | Equipment for collecting microorganisms from fluid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220526504 CN203065483U (en) | 2012-10-15 | 2012-10-15 | Equipment for collecting microorganisms from fluid |
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| Publication Number | Publication Date |
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| CN203065483U true CN203065483U (en) | 2013-07-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201220526504 Expired - Lifetime CN203065483U (en) | 2012-10-15 | 2012-10-15 | Equipment for collecting microorganisms from fluid |
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| CN (1) | CN203065483U (en) |
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2012
- 2012-10-15 CN CN 201220526504 patent/CN203065483U/en not_active Expired - Lifetime
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Address after: 518083 11F-3, Beishan industrial complex, 146 Beishan Road, Yantian District, Shenzhen, Guangdong Patentee after: BGI SHENZHEN Co.,Ltd. Patentee after: BGI SHENZHEN Address before: 518083 11F-3, Beishan industrial complex, 146 Beishan Road, Yantian District, Shenzhen, Guangdong Patentee before: BGI SHENZHEN Co.,Ltd. Patentee before: BGI SHENZHEN |
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Granted publication date: 20130717 |