CN203053870U - Device for carrying out multichannel dry-type detection on glutamic-pyruvic transaminase and haemoglobin - Google Patents
Device for carrying out multichannel dry-type detection on glutamic-pyruvic transaminase and haemoglobin Download PDFInfo
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- CN203053870U CN203053870U CN 201220736328 CN201220736328U CN203053870U CN 203053870 U CN203053870 U CN 203053870U CN 201220736328 CN201220736328 CN 201220736328 CN 201220736328 U CN201220736328 U CN 201220736328U CN 203053870 U CN203053870 U CN 203053870U
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Abstract
The utility model relates to a device for detecting the biochemical index of a human body, and particularly relates to a device for carrying out multichannel dry-type detection on glutamic-pyruvic transaminase and haemoglobin. The device disclosed by the utility model can carry out multi-sample simultaneous detection on two items of the glutamic-pyruvic transaminase and the haemoglobin and has the advantages of high detection speed, high efficiency, easiness and convenience for operation, easiness for manufacture and lower relative cost. The device comprises a bottom lining card, wherein the bottom lining card is provided with a sampling loading filter region, a sample flow mat, lug bosses, a sampling loading region and a secondary reaction region; the sampling loading filter region contacts with the sample flow mat; the lug bosses are all provided with first reaction regions; the first reaction regions all comprise at least one group of reaction films; one ends of the reaction films of the first reaction regions are fixed on the lug bosses, and the other ends of the reaction films of the first reaction regions are positioned above the sample flow mat; the sampling loading region is communicated with the secondary reaction region and is provided with an air port in the secondary reaction region; and the secondary reaction region is internally provided with a reaction reagent and a lysis reagent.
Description
Technical field
The utility model relates to a kind of for the device that the human body biochemical indicator is detected, and particularly relates to a kind of glutamic-pyruvic transaminase and haemoglobin being carried out the device that the hyperchannel dry type detects.
Background technology
As everyone knows, glutamic-pyruvic transaminase and haemoglobin hyperchannel dry type pick-up unit are the devices that a kind of glutamic-pyruvic transaminase to human body and haemoglobin detect, haemoglobin is a kind of protein of being responsible for delivery oxygen in the human body, constituted by four subunits, each subunit contains a heme group, haemoglobin is as the important substance of finishing the exchange of oxygen and carbon dioxide in the blood, it is the pacing items of the activity of earning a bare living, therefore the haemoglobin detection is the most important index of diagnosing severity of anemia clinically, also is the hemopathic important indicators of auxiliary diagnosis other types simultaneously; Glutamic-pyruvic transaminase (has another name called glutamate transaminase, be called for short GPT, ALT), whether the liver of glutamic-pyruvic transaminase key reaction human body is normal, and liver is the detoxifcation organ of human body maximum, and whether normal these internal organs are, is very important concerning human body, it is the important indicator that liver function goes wrong that liver GPT raises, in common factor, all kinds of hepatitis can cause that GPT raises, so whether whether the numerical value of glutamic-pyruvic transaminase normally directly can be used for diagnosing hepatic normal.
Existing detection for glutamic-pyruvic transaminase and haemoglobin all is separate detection, and its program is very complicated, and detection technique and its defective to haemoglobin under the prior art condition are as follows:
1, cyanmethemoglobin method (HiCN method), this law technology route is: use the surfactant triton x-100 as molten born of the same parents' agent, splitting erythrocyte discharges haemoglobin.Haemoglobin is oxidized to methemoglobin through high-potassium ferricyanide, and methemoglobin generates cyanmethemoglobin with the potassium cyanide reaction again, has specific absorption at the 540nm place, calculates the content of haemoglobin through colourimetry; This method accuracy, precision are the highest, but operation is comparatively loaded down with trivial details, need to use toxic articles such as potassium cyanide in the checkout procedure, and human body and environment are all damaged, and go up and should not large tracts of land promote the use of so use clinically.
2, routine clinical detection method, this law technology route is: use molten born of the same parents' agent (as triton x-100) splitting erythrocyte, discharge haemoglobin.Haemoglobin and lauryl sodium sulfate (SLS) reaction generates lauryl sodium sulfate haemoglobin (SLS-Hb) brown compound, has specific absorption at the 540nm place, calculates the content of haemoglobin through colourimetry; This method has automaticity height, accuracy precision height, pollution is little, toxicity is low, detect advantages such as with low cost, but also exist operation need professional training, matched automation equipment not portable, be unfavorable for fast detecting and bedside diagnosis (POCT), wait shortcoming more slowly with respect to dry chemical method detection speed.
3, dry chemical method, dry chemical method are to detect haemoglobin method comparatively quickly and easily, and the detection principle of this method has all adopted azide methemoglobin method relative theory, and its clinical practice is limited to health check-up examination and bedside diagnosis fields such as (POCT) substantially.Be characterized in detection speed fast (about 15 seconds/person-portion), simple to operate, portable, but each test strips (analoids) can only detect a sample at every turn, and detection efficiency is on the low side relatively.
And it is also roughly identical with its defective with detection technique to haemoglobin to the detection technique of glutamic-pyruvic transaminase and its defective under the prior art.
The utility model content
For solving the problems of the technologies described above, the utility model provides a kind of can be simultaneously to carry out multisample to glutamic-pyruvic transaminase and two projects of haemoglobin and detects simultaneously, and detection speed is fast, efficient is high, easy and simple to handle, make simple and lower glutamic-pyruvic transaminase and the haemoglobin hyperchannel dry type pick-up unit of relative cost.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit, comprise the end liner card, described end liner card is provided with a plurality of application of sample filtrating areas, a plurality of sample flow pad, a plurality of projection, a plurality of sample application zone and a plurality of second reaction zone, and the quantity of described application of sample filtrating area, sample flow pad and projection is identical, and described sample application zone is identical with the quantity of second reaction zone; Each application of sample filtrating area in described a plurality of application of sample filtrating area all contacts with a sample flow pad, be provided with first reaction zone in described a plurality of projection on each projection, and each first reaction zone includes at least one group reaction film, one end of the reaction film of each first reaction zone is fixed on the projection, and the other end of the reaction film of each first reaction zone is positioned at the top of a sample flow pad; Each sample application zone in described a plurality of sample application zone all is communicated with second reaction zone, and is provided with blow vent at each second reaction zone, is provided with reaction reagent and molten born of the same parents' reagent in each second reaction zone.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit, described application of sample filtrating area comprises diffusion layer, sample pad and hemofiltration film, and described diffusion layer is positioned at the top of sample pad, and described sample pad is positioned at the top of hemofiltration film; Described diffusion layer, sample pad and hemofiltration film are bonded together by bonding piece, and described diffusion layer, sample pad and hemofiltration film be bonded on the end liner card by bonding piece, and described sample flow pad is positioned at the below of described hemofiltration film.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit also comprise cover plate, and described cover plate is fixedly mounted on the top of described end liner card, and described cover plate is provided with the hole of arranging in pairs or groups mutually with application of sample filtrating area and reaction zone.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit, described cover plate fastens together by buckle and end liner.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit, described first reaction zone comprises many group reactions film, and the stack of many group reactions film is fixed together.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit, described sample application zone and described second reaction zone pass through channel connection.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit, described second reaction zone comprises reaction groove and reaction cover plate, described reaction cover plate lid is contained on the described reaction groove, and described reaction reagent or molten born of the same parents' reagent are positioned at described reaction groove, the open rearward end of described reaction groove, and by described opening and channel connection, described blow vent is positioned on the front end face of described reaction groove.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit, the degree of depth of described reaction groove is 0.2mm to 0.8mm, described reaction cover plate is fixed together by the ultrasonic soldering mode with the reaction groove.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit, described sample application zone comprise appearance sample groove, and described appearance sample groove is communicated with second reaction zone, and appearance sample groove increases gradually from going into the degree of depth of sample end to sample outlet end.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit, described end liner card is made by the high rigidity hydrophobic polymeric material, and described high rigidity hydrophobic polymeric material is tygon or Polyvinylchloride or polypropylene or polycarbonate or organic glass.
Compared with prior art the beneficial effects of the utility model are: be provided with the end liner card, and the end liner card is provided with a plurality of application of sample filtrating areas, a plurality of sample flow pad, a plurality of projection, a plurality of sample application zone and a plurality of second reaction zone, and the quantity of application of sample filtrating area, sample flow pad and projection is identical, and sample application zone is identical with the quantity of second reaction zone; Each application of sample filtrating area in a plurality of application of sample filtrating areas all contacts with a sample flow pad, be provided with first reaction zone in a plurality of projections on each projection, and each first reaction zone includes at least one group reaction film, one end of the reaction film of each first reaction zone is fixed on the projection, and the other end of the reaction film of each first reaction zone is positioned at the top of a sample flow pad; Each sample application zone in a plurality of sample application zone all is communicated with second reaction zone, and is provided with blow vent at each second reaction zone, is provided with reaction reagent and molten born of the same parents' reagent in each second reaction zone; Like this, the detection mode of glutamic-pyruvic transaminase is as follows: carry out the filtration of blood sample by the application of sample filtrating area, whole blood at the application of sample filtrating area after filtering, in the serum (or blood plasma) measured object with serum (or blood plasma) below the sample flow pad is passed to each first reaction zone, control through the particular detection instrument, press down the reaction film of first reaction zone, reaction film contacts with serum (or blood plasma) on the sample flow pad, measured object in the serum (or blood plasma) with chromogenic reaction can take place after the chemical reagent on the reaction film contacts, the reaction film variable color, again by the optical detection elements (light source on the particular detection instrument, sensor etc.), change color on the detection reaction film, and then calculate the concentration of measured object; The detection mode of haemoglobin is as follows: after blood sample adds sample application zone, just can suck the second reaction zone inside by capillary action, red blood cell in the blood sample in second reaction zone through the cracking of molten born of the same parents' reagent, discharge haemoglobin, generate specific color with the reaction reagent reaction more afterwards, change color through particular detection instrument detection reaction district, and then calculate the concentration of measured object haemoglobin; Above-mentioned testing process can be simultaneously carried out multisample to glutamic-pyruvic transaminase and two projects of haemoglobin and is detected simultaneously, and detection speed is fast, efficient is high, easy and simple to handle, make simple and relative cost lower.
Description of drawings
Fig. 1 is plan structure synoptic diagram of the present utility model;
Fig. 2 is the structural representation of the utility model application of sample filtrating area, sample flow pad and projection annexation;
Fig. 3 is the structural representation of the utility model sample application zone and second reaction zone.
Embodiment
Below in conjunction with drawings and Examples, embodiment of the present utility model is described in further detail.Following examples are used for explanation the utility model, but are not used for limiting scope of the present utility model.
As shown in Figure 1 to Figure 3, glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit, comprise end liner card 1, the end liner card is provided with a plurality of application of sample filtrating areas 2, a plurality of sample flow pad 3, a plurality of projection 4, a plurality of sample application zone 6 and a plurality of second reaction zone 7, and the quantity of application of sample filtrating area, sample flow pad and projection is identical, and sample application zone is identical with the quantity of second reaction zone; Each application of sample filtrating area in a plurality of application of sample filtrating areas all contacts with a sample flow pad, be provided with first reaction zone 5 in a plurality of projections on each projection, and each first reaction zone includes at least one group reaction film, one end of the reaction film of each first reaction zone is fixed on the projection, and the other end of the reaction film of each first reaction zone is positioned at the top of a sample flow pad; Each sample application zone in a plurality of sample application zone all is communicated with second reaction zone, and is provided with blow vent 8 at each second reaction zone, is provided with reaction reagent and molten born of the same parents' reagent in each second reaction zone; Like this, the detection mode of glutamic-pyruvic transaminase is as follows: carry out the filtration of blood sample by the application of sample filtrating area, whole blood at the application of sample filtrating area after filtering, in the serum (or blood plasma) measured object with serum (or blood plasma) below the sample flow pad is passed to each first reaction zone, control through the particular detection instrument, press down the reaction film of first reaction zone, reaction film contacts with serum (or blood plasma) on the sample flow pad, measured object in the serum (or blood plasma) with chromogenic reaction can take place after the chemical reagent on the reaction film contacts, the reaction film variable color, again by the optical detection elements (light source on the particular detection instrument, sensor etc.), change color on the detection reaction film, and then calculate the concentration of measured object; The detection mode of haemoglobin is as follows: after blood sample adds sample application zone, just can suck the second reaction zone inside by capillary action, red blood cell in the blood sample in second reaction zone through the cracking of molten born of the same parents' reagent, discharge haemoglobin, generate specific color with the reaction reagent reaction more afterwards, change color through particular detection instrument detection reaction district, and then calculate the concentration of measured object haemoglobin; Above-mentioned testing process can be simultaneously carried out multisample to glutamic-pyruvic transaminase and two projects of haemoglobin and is detected simultaneously, and detection speed is fast, efficient is high, easy and simple to handle, make simple and relative cost lower.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit, the application of sample filtrating area comprises diffusion layer 21, sample pad 22 and hemofiltration film 23, and diffusion layer is positioned at the top of sample pad, and sample pad is positioned at the top of hemofiltration film; Diffusion layer, sample pad and hemofiltration film are bonded together by bonding piece, and diffusion layer, sample pad and hemofiltration film are bonded on the end liner card by bonding piece 24, the sample flow pad is positioned at the below of hemofiltration film, the application of sample filtrating area is in order to carry out filtration treatment to whole blood sample herein, separate and obtain serum (or blood plasma), can select commercially available sample loading material as required, the whole blood filtering material, as the glass fiber material in different apertures etc., diffusion layer in the application of sample filtrating area, sample pad and hemofiltration film can be according to detecting needs, and curing or bag are by the chemical reagent relevant with test reaction or auxiliary reagent; Like this, can reach the purpose of optimizing reaction system or promotion serum sample flowability.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit also comprise cover plate, and cover plate is fixedly mounted on the top of end liner card, and on the cover board are provided with the hole of arranging in pairs or groups mutually with application of sample filtrating area and reaction zone; Cover plate prevents from coming off in order to fastening application of sample filtrating area, sample flow pad, reaction zone, all designs the hole so that application of sample operation and instrument detect at application of sample filtrating area and each reaction zone center.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit, cover plate fastens together by buckle and end liner, and like this, the chucking of being more convenient for is fixing.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit, first reaction zone comprises many group reactions film, and the stack of many group reactions film is fixed together.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit, in the design of first reaction zone and reaction film, the quantity of reaction film is carried out design alternative according to the test item needs, and first reaction zone is set all on each projection, like this, each first reaction zone that forms in a plurality of first reaction zones detects a physiochemical indice, so a pick-up unit can be bled to one simultaneously and be carried out the many index detection, the reaction film of mentioning in the utility model is a kind of macromolecule member material, in order to the required main chemical reagent of fixing bearing test reaction.Its material can but be not limited to commercially available nitrocellulose filter, polysulfone membrane, polyester film, polycarbonate membrane, all-glass paper etc.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit, diffusion layer, sample pad and hemofiltration film have used the glass fiber material of different apertures and thickness respectively, wherein wrap in advance by auxiliary reagents such as surfactants on the sample pad, the sample flow pad is a kind of very thin glass fiber material in the present embodiment, be cut into tee T, one end contacts with the sample application zone stack, the serum (or blood plasma) that obtains after filtering of absorption whole blood sample, earlier after vertical transmission again lateral transport to each reaction zone.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit, sample application zone is communicated with by passage 9 with second reaction zone.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit, second reaction zone comprises reaction groove and reaction cover plate 10 (the reaction cover plate is a very thin transparent or semitransparent film in the utility model), reaction cover plate lid is contained on the reaction groove, and reaction reagent or molten born of the same parents' reagent are positioned at the reaction groove, the open rearward end of reaction groove, and by opening and channel connection, blow vent is positioned on the front end face of reaction groove, like this, can be more convenient for sucking the second reaction zone inside by capillary action after blood sample adds sample application zone.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit, the degree of depth of reaction groove is 0.2mm to 0.8mm, the reaction cover plate is fixed together by the ultrasonic soldering mode with the reaction groove.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit, sample application zone comprises holds the sample groove, holding the sample groove is communicated with second reaction zone, and holding the sample groove increases gradually from going into the degree of depth of sample end to sample outlet end, like this, form a natural grade, can make blood sample enter to second reaction zone from sample application zone by the gravity effect.
Glutamic-pyruvic transaminase of the present utility model and haemoglobin hyperchannel dry type pick-up unit, the end liner card is made by the high rigidity hydrophobic polymeric material, and the high rigidity hydrophobic polymeric material be tygon or Polyvinylchloride or polypropylene or polycarbonate or organic glass, in embodiment of the present utility model, then mainly adopt polycarbonate as making material; Certainly the above-mentioned material design that includes but not limited to the end liner card, above-mentioned cover plate and reaction cover plate also can be made by above-mentioned material.
The above only is preferred implementation of the present utility model; should be understood that; for those skilled in the art; under the prerequisite that does not break away from the utility model know-why; can also make some improvement and modification, these improve and modification also should be considered as protection domain of the present utility model.
Claims (10)
1. a glutamic-pyruvic transaminase and haemoglobin hyperchannel dry type pick-up unit, it is characterized in that, comprise the end liner card, described end liner card is provided with a plurality of application of sample filtrating areas, a plurality of sample flow pad, a plurality of projection, a plurality of sample application zone and a plurality of second reaction zone, and the quantity of described application of sample filtrating area, sample flow pad and projection is identical, and described sample application zone is identical with the quantity of second reaction zone; Each application of sample filtrating area in described a plurality of application of sample filtrating area all contacts with a sample flow pad, be provided with first reaction zone in described a plurality of projection on each projection, and each first reaction zone includes at least one group reaction film, one end of the reaction film of each first reaction zone is fixed on the projection, and the other end of the reaction film of each first reaction zone is positioned at the top of a sample flow pad; Each sample application zone in described a plurality of sample application zone all is communicated with second reaction zone, and is provided with blow vent at each second reaction zone, is provided with reaction reagent and molten born of the same parents' reagent in each second reaction zone.
2. glutamic-pyruvic transaminase as claimed in claim 1 and haemoglobin hyperchannel dry type pick-up unit, it is characterized in that, described application of sample filtrating area comprises diffusion layer, sample pad and hemofiltration film, and described diffusion layer is positioned at the top of sample pad, and described sample pad is positioned at the top of hemofiltration film; Described diffusion layer, sample pad and hemofiltration film are bonded together by bonding piece, and described diffusion layer, sample pad and hemofiltration film be bonded on the end liner card by bonding piece, and described sample flow pad is positioned at the below of described hemofiltration film.
3. glutamic-pyruvic transaminase as claimed in claim 2 and haemoglobin hyperchannel dry type pick-up unit, it is characterized in that, also comprise cover plate, described cover plate is fixedly mounted on the top of described end liner card, and described cover plate is provided with the hole of arranging in pairs or groups mutually with application of sample filtrating area and reaction zone.
4. glutamic-pyruvic transaminase as claimed in claim 3 and haemoglobin hyperchannel dry type pick-up unit is characterized in that described cover plate fastens together by buckle and end liner.
5. glutamic-pyruvic transaminase as claimed in claim 4 and haemoglobin hyperchannel dry type pick-up unit is characterized in that described first reaction zone comprises many group reactions film, and the stack of many group reactions film is fixed together.
6. glutamic-pyruvic transaminase as claimed in claim 1 and haemoglobin hyperchannel dry type pick-up unit is characterized in that, described sample application zone and described second reaction zone pass through channel connection.
7. glutamic-pyruvic transaminase as claimed in claim 6 and haemoglobin hyperchannel dry type pick-up unit, it is characterized in that, described second reaction zone comprises reaction groove and reaction cover plate, described reaction cover plate lid is contained on the described reaction groove, and described reaction reagent or molten born of the same parents' reagent are positioned at described reaction groove, the open rearward end of described reaction groove, and by described opening and channel connection, described blow vent is positioned on the front end face of described reaction groove.
8. glutamic-pyruvic transaminase as claimed in claim 7 and haemoglobin hyperchannel dry type pick-up unit is characterized in that the degree of depth of described reaction groove is 0.2mm to 0.8mm, and described reaction cover plate is fixed together by the ultrasonic soldering mode with the reaction groove.
9. glutamic-pyruvic transaminase as claimed in claim 8 and haemoglobin hyperchannel dry type pick-up unit, it is characterized in that, described sample application zone comprises holds the sample groove, and described appearance sample groove is communicated with second reaction zone, and appearance sample groove increases gradually from going into the degree of depth of sample end to sample outlet end.
10. as each described glutamic-pyruvic transaminase of claim 1 to 9 and haemoglobin hyperchannel dry type pick-up unit, it is characterized in that, described end liner card is made by the high rigidity hydrophobic polymeric material, and described high rigidity hydrophobic polymeric material is tygon or Polyvinylchloride or polypropylene or polycarbonate or organic glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220736328 CN203053870U (en) | 2012-12-28 | 2012-12-28 | Device for carrying out multichannel dry-type detection on glutamic-pyruvic transaminase and haemoglobin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220736328 CN203053870U (en) | 2012-12-28 | 2012-12-28 | Device for carrying out multichannel dry-type detection on glutamic-pyruvic transaminase and haemoglobin |
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| CN203053870U true CN203053870U (en) | 2013-07-10 |
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| CN 201220736328 Expired - Fee Related CN203053870U (en) | 2012-12-28 | 2012-12-28 | Device for carrying out multichannel dry-type detection on glutamic-pyruvic transaminase and haemoglobin |
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| CN (1) | CN203053870U (en) |
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- 2012-12-28 CN CN 201220736328 patent/CN203053870U/en not_active Expired - Fee Related
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