CN203048945U - Disc type superspeed real-time fluorescence quantification polymerase chain reaction (PCR) instrument - Google Patents
Disc type superspeed real-time fluorescence quantification polymerase chain reaction (PCR) instrument Download PDFInfo
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- CN203048945U CN203048945U CN 201320086198 CN201320086198U CN203048945U CN 203048945 U CN203048945 U CN 203048945U CN 201320086198 CN201320086198 CN 201320086198 CN 201320086198 U CN201320086198 U CN 201320086198U CN 203048945 U CN203048945 U CN 203048945U
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- instrument
- disc type
- pcr
- time fluorescence
- detection
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Abstract
The utility model discloses a disc type superspeed real-time fluorescence quantification polymerase chain reaction (PCR) instrument. The instrument comprises an analysis meter body, wherein a sample loading tray, a heating disc and a sealing device are arranged in the analysis meter body, and the heating disc is connected with a fluorescence detector. The instrument has the beneficial effects that the instrument is of a constant temperature heating type, the reaction speed is greatly increased, the whole testing time is shortened, and the instrument can be conveniently applied to clinical detection and particularly sudden epidemical detection. The disc type sample loading tray and a sealing tray are used, so that reaction and detection processes are leakproof, and PCR contamination is effectively avoided. The PCR circulation is realized through high speed rotating discs, and the instrument is simpler and more effective than a vertical or left-right shifting mode; and a mechanical structure and a control circuit are simplified, so that mechanical faults are reduced, and the detection cost is also lowered.
Description
Technical field
The utility model relates to a kind of PCR instrument, particularly a kind of disc type ultra-high speed real-time fluorescence quantitative PCR instrument.
Background technology
Fa Ming polymerase chain reaction makes that people can be at the specific nucleic acid fragment of external autotelic unlimited amplification before, and its principle is similar in the body of DNA and copies, and external synthetic a kind of conditions suitable that provides of DNA just is provided in centrifuge tube.The appearance of PCR instrument then makes this technology realize automatization, and it is also more extensive to make the technology of PCR use, but the technology of PCR also is faced with many challenges, particularly in the nucleic acid real time fluorescent quantitative context of detection of clinical disease substance.Because all there is heating and cooling process in existing P CR instrument, whole proliferation time is long, also more complicated of amplification procedure, and the result is not easy repetition, and especially the context of detection in sudden transmissible disease is restricted.
The utility model content
Technical problem to be solved in the utility model is at the deficiencies in the prior art, and a kind of disc type ultra-high speed real-time fluorescence quantitative PCR instrument is provided.
To achieve these goals, the measure taked of the utility model:
A kind of disc type ultra-high speed real-time fluorescence quantitative PCR instrument comprises the analyser body, is provided with an application of sample dish, a heating plate and a tightness system in the described analyser body, and heating plate links to each other with fluorimetric detector.
Be provided with reaction of degeneration district, extension district and annealing reaction district in the described heating plate.
Described application of sample dish and tightness system are provided with several wells and plate fixed orifices.
Described application of sample dish, heating plate and tightness system are supporting onesize disk.
The beneficial effects of the utility model: the utility model is fixed temperature formula heating type, and speed of response improves greatly, has shortened whole detection time, can be applied to the detection of clinical detection, particularly sudden transmissible disease more easily.The application of sample dish of disc type, the use of sealing pad has also realized the stopping property in reaction and the testing process, has effectively prevented the pollution of PCR.Realize the circulation of PCR by the high-speed rotation of disk, compared with up and down or about move mode simpler effectively, physical construction and control circuit are more simplified, and have so just reduced mechanical fault, have also reduced the detection cost.
Description of drawings
Fig. 1, structural representation of the present utility model.
Embodiment
A kind of disc type ultra-high speed real-time fluorescence quantitative PCR instrument comprises analyser body 1, is provided with an application of sample dish 2, a heating plate 3 and a tightness system 5 in the described analyser body 1, and heating plate 3 links to each other with fluorimetric detector 4.
Be provided with reaction of degeneration district 6, extension district 7 and annealing reaction district 8 in the described heating plate 3.
Described application of sample dish 2 and tightness system 5 are provided with several wells 9 and plate fixed orifices 10.
Described application of sample dish 2, heating plate 3 and tightness system 5 are supporting onesize disk.
During detection, in the well 9 in the PCR reaction solution adding application of sample dish 2 for preparing, after all wells all add well, cover good seal with tightness system 5, position by plate fixed orifices 10 is placed into above the heating plate 3 again, application of sample dish 2 and heating plate 3 are combined closely fixing, set the temperature of each heating zone, when application of sample dish 2 in the reaction of degeneration district 6 of circular distribution, when 8 three the different not stalls of humidity province in extension district 7 and annealing reaction district are moving, finish the PCR circulation, when application of sample dish 2 rotated, fluorimetric detector 4 was finished the detection of amplified production fluorescence intensity synchronously.
Those skilled in the art's simple change and replacement in this area all are within the protection domain of the present utility model.
Claims (4)
1. disc type ultra-high speed real-time fluorescence quantitative PCR instrument, comprise analyser body (1), it is characterized in that: be provided with an application of sample dish (2), a heating plate (3) and a tightness system (5) in the described analyser body (1), heating plate (3) links to each other with fluorimetric detector (4).
2. disc type ultra-high speed real-time fluorescence quantitative PCR instrument according to claim 1 is characterized in that: be provided with reaction of degeneration district (6), extension district (7) and annealing reaction district (8) in the described heating plate (3).
3. disc type ultra-high speed real-time fluorescence quantitative PCR instrument according to claim 1, it is characterized in that: described application of sample dish (2) and tightness system (5) are provided with several wells (9) and plate fixed orifices (10).
4. disc type ultra-high speed real-time fluorescence quantitative PCR instrument according to claim 1, it is characterized in that: described application of sample dish (2), heating plate (3) and tightness system (5) are supporting onesize disk.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320086198 CN203048945U (en) | 2013-01-31 | 2013-01-31 | Disc type superspeed real-time fluorescence quantification polymerase chain reaction (PCR) instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320086198 CN203048945U (en) | 2013-01-31 | 2013-01-31 | Disc type superspeed real-time fluorescence quantification polymerase chain reaction (PCR) instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203048945U true CN203048945U (en) | 2013-07-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320086198 Expired - Fee Related CN203048945U (en) | 2013-01-31 | 2013-01-31 | Disc type superspeed real-time fluorescence quantification polymerase chain reaction (PCR) instrument |
Country Status (1)
| Country | Link |
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| CN (1) | CN203048945U (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103695309A (en) * | 2013-12-19 | 2014-04-02 | 江苏金太生命科技有限公司 | Novel PCR (Polymerase Chain Reaction) instrument |
| CN103820316B (en) * | 2014-03-09 | 2015-12-30 | 北京工业大学 | Based on the real-time PCR detection system of rotary micro-fluidic chip |
| WO2018194240A1 (en) * | 2017-04-17 | 2018-10-25 | (주)레보스케치 | Digital pcr device and method using centrifugal force |
| CN111607509A (en) * | 2019-02-22 | 2020-09-01 | 西安天隆科技有限公司 | Automatic amplification and detection device for digital PCR |
| CN112159756A (en) * | 2020-09-16 | 2021-01-01 | 复旦大学 | Rapid PCR amplification and CRISPR visual detection device and detection method |
| CN114480733A (en) * | 2021-12-29 | 2022-05-13 | 广东国盛医学科技有限公司 | Virus detection method and detection system thereof |
-
2013
- 2013-01-31 CN CN 201320086198 patent/CN203048945U/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103695309A (en) * | 2013-12-19 | 2014-04-02 | 江苏金太生命科技有限公司 | Novel PCR (Polymerase Chain Reaction) instrument |
| CN103820316B (en) * | 2014-03-09 | 2015-12-30 | 北京工业大学 | Based on the real-time PCR detection system of rotary micro-fluidic chip |
| WO2018194240A1 (en) * | 2017-04-17 | 2018-10-25 | (주)레보스케치 | Digital pcr device and method using centrifugal force |
| US11260393B2 (en) | 2017-04-17 | 2022-03-01 | Revosketch Co., Ltd. | Digital PCR device and method using centrifugal force |
| CN111607509A (en) * | 2019-02-22 | 2020-09-01 | 西安天隆科技有限公司 | Automatic amplification and detection device for digital PCR |
| CN111607509B (en) * | 2019-02-22 | 2023-04-07 | 西安天隆科技有限公司 | Automatic amplification and detection device for digital PCR |
| CN112159756A (en) * | 2020-09-16 | 2021-01-01 | 复旦大学 | Rapid PCR amplification and CRISPR visual detection device and detection method |
| CN114480733A (en) * | 2021-12-29 | 2022-05-13 | 广东国盛医学科技有限公司 | Virus detection method and detection system thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| DD01 | Delivery of document by public notice |
Addressee: Hefei Yalai Bio-engineering Co., Ltd. Document name: Notification to Pay the Fees |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130710 Termination date: 20150131 |
|
| EXPY | Termination of patent right or utility model |