CN203048942U - Device for monitoring color of liquid - Google Patents
Device for monitoring color of liquid Download PDFInfo
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- CN203048942U CN203048942U CN 201220530901 CN201220530901U CN203048942U CN 203048942 U CN203048942 U CN 203048942U CN 201220530901 CN201220530901 CN 201220530901 CN 201220530901 U CN201220530901 U CN 201220530901U CN 203048942 U CN203048942 U CN 203048942U
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- test container
- transparent test
- monitoring device
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Abstract
The utility model discloses a device for monitoring the color of the liquid. The device comprises a transparent test container, a sensor arranged close to the transparent test container, and a processing module electrically connected with the sensor, wherein the sensor comprises a camera shooting module with the pick-up lens facing towards the transparent test container, and a power supply arranged close to the transparent test container; and the power supply and the camera shooting module are oppositely arranged at two sides of the transparent test container, and a diverging lens is arranged at one side of the power supply close to the transparent test container. All the parts coordinate together, so that the color of the liquid can be accurately monitored without destroying the sealing environment in which the liquid is located.
Description
Technical field
The utility model relates to a kind of monitoring device, particularly relates to a kind of liquid color monitoring device.
Background technology
The T cell is important white cell, assists the immune response of antagonism cancer and virus infection.They produce in thymus gland, and long-term immunological memory is arranged, and need in order to quick response in the immune response.The T cell is in case by antigen activates, can quick copy, produce antigen-reactive rapidly.The T cell that handle is lived from people's peripheral blood separately is possible with other hemocytes.These T cells can be cultivated in external hollow fiber conduit, at external rapid amplifying.Hollow fiber conduit is the part of the cell culture system of automatization.By contact specific antigens and the normal activated immune cell factor, these T cells can be activated external, become the instrument of more effective strike disease.This process is called as amplification and the activation of T cell.In case the T cell is amplified and activates external, they can be transfused in the body of getting back to former patient.One of key feature of automatic cell culture system is a kind of mechanism that can exchange nutrient solution automatically.Nutrient solution is the culturing cell requisite a kind of nutrition " soup " of growing.Along with the growth of cell, oxygen and nutritive substance in the nutrient solution are consumed, and refuse and carbonic acid gas that cellular metabolism produces increase in nutrient solution.If the nutriment undersupply, the growth of cell will be affected, even stops growing, and continues to be exposed in the nutrient solution of normal body temperature (37 ℃) and scarce nutriment, will cause necrocytosis.
Generally, cell cultures needs the technician to monitor glucose concn and liquid color in the nutrient solution, to determine the freshness of nutrient solution.When the nutrient in the nutrient solution is reduced to a certain degree, must manually change nutrient solution.These operations must be carried out in gnotobasis, and the laminar flow hood of high efficiency particle air filter need be arranged, the vest of barrier and uv irradiating light source.Each artificial nutrient solution of changing all might be introduced bacterium, causes the risk of loss cell.Generally all add phenol red dyestuff as the pH indicator of solution in the nutrient solution commonly used.The transmission spectrophotometer is used in the assay determination of solution liquid color, reads phenol red dyestuff at the absorbance value of red and yellow wavelengths.The high definition quartz colorimetric utensil is commonly used to carry out this analysis.The sealed environment that this method requires to break cell cultures is taken a sample, and can bring bacterium is incorporated into risk in the cell culture environment thereupon.
Summary of the invention
Technical problem to be solved in the utility model is, provide a kind of can be under the prerequisite of the sealed environment of not breaking the liquid place device of precise monitoring liquid color.
For solving above technical problem, the technical solution of the utility model is: a kind of liquid color monitoring device, comprise transparent test container, its key is: also comprise being arranged near the sensor of described transparent test container and the processing module that electrically is connected with described sensor.
As improvement one of the present utility model, described transparent test container and described sensor are installed in the lucifuge case.
As improvement two of the present utility model, described transparent test container is transparent platinum solidification of silicon sebific duct.
Further improve as the utility model, the external diameter of described transparent platinum solidification of silicon sebific duct is more than or equal to 3 millimeters, and the thickness of described transparent platinum solidification of silicon sebific duct tube wall is no more than 25% of described transparent platinum solidification of silicon sebific duct external diameter diameter.
As improvement three of the present utility model, described sensor comprises that pick-up lens is towards the photographing module of described transparent test container.
Further improve as the utility model, described sensor also comprises near the light source that is arranged on the described transparent test container.
Further improve as the utility model, described light source and described photographing module are oppositely arranged on the both sides of described transparent test container.
Further improve again as the utility model, also be provided with the diverging lens utility model at described light source near a side of described transparent test container.
Preferably, the camera lens of described photographing module is high-resolution CMOS camera.
By implement the utility model desirable following beneficial effect:
A kind of liquid color monitoring device comprises transparent test container, also comprises being arranged near the sensor of described transparent test container and the processing module that electrically is connected with described sensor.The tube wall that described sensor sees through described transparent test container obtains the color of solution and this colouring information is passed to processing module, and processing module is judged signal by the outside output of comparison.This structure guaranteed under the prerequisite of the sealed environment of not breaking the liquid place can precise monitoring liquid color, this monitoring device is applied in the cell auto culturing system, not only allow this operation of monitoring liquid color easy handling that becomes, but also guaranteed that cell cultures can not be affected owing to introducing extraneous bacterium.Described sensor comprises pick-up lens over against the photographing module of described transparent test container, and described sensor also comprises near the light source that is arranged on the described transparent test container, and described photographing module and light source are oppositely arranged on the both sides of described transparent test container.Light source illuminates to overcome the interference of surround lighting with described transparent test container, thus guarantee solution in the transparent test container can be in the camera lens of described photographing module accurately image, make that the monitoring accuracy of monitoring device is higher.Described test container and described sensor are installed in the lucifuge case, and the lucifuge case has been stopped extraneous light to the influence of monitoring treating processes.Side at the close described transparent test container of described light source also is provided with diverging lens, and the light that diverging lens sends by light source is radiated on the described transparent test container more equably, has avoided owing to the even imaging problem of dtmf distortion DTMF that causes of uneven illumination.The camera lens of described photographing module is high-resolution CMOS camera, adopts the CMOS camera to greatly reduce the manufacturing cost of monitoring device, thereby makes this device to be widely used.Owing to disposed corresponding light source to photographing module, when assurance is cheap for manufacturing cost, guaranteed the image quality of photographing module again simultaneously.
Described transparent test container is transparent platinum solidification of silicon sebific duct, usually the cultivation pipeline in the cell auto culturing system can adopt transparent platinum solidification of silicon sebific duct, if transparent test container adopts transparent platinum solidification of silicon sebific duct, just can directly transparent test container be incorporated in the cell auto culturing system, be made of one structure with the cultivation pipeline in the cell auto culturing system, directly the cultivation pipeline with the cell auto culturing system substitutes transparent test container.When the nutrient solution in the cell auto culturing system is monitored, just need not in addition transparent test container be linked in the described auto culturing system like this, directly the pipeline of auto culturing system be monitored, allow the monitoring operation more simply be convenient to operation.The external diameter of described transparent platinum solidification of silicon sebific duct is more than or equal to 3 millimeters, the thickness of described transparent platinum solidification of silicon sebific duct tube wall is no more than 25% of described transparent platinum solidification of silicon sebific duct external diameter diameter, can avoid like this causing into image distortion because of refraction and/or reflection, make monitoring result more accurate.
Description of drawings
The utility model is described in more detail below in conjunction with Figure of description:
Fig. 1 is cell cultures plumbing installation structural representation;
Fig. 2 is cell automatization culture systems structural representation;
Fig. 3 is liquid color monitoring device structural representation;
Fig. 4 is one of culture tube transporter structural representation;
Fig. 5 is two of culture tube transporter structural representation.
Embodiment
As shown in Figure 1, a kind of cell cultures plumbing installation that the utility model can be applied thereon, comprise the main circulator 20, gaseous interchange pipe 21, culture tube 23 and the nutrient solution master library 19 that connect successively by thrust-augmenting nozzle, described nutrient solution master library 19 is provided with a micropore diameter venting hole 191 that is connected with atmosphere, thereby can realize that described nutrient solution master library 19 and external atmosphere pressure keep balance, guarantee the smoothly circulation cycle of nutrient solution in the cell cultures plumbing installation.This simple structure has been formed a complete cell culture apparatus, and main circulator 20 drives nutrient solution and flows in whole loop.Because described gaseous interchange pipe 21 is equivalent to artificial lung, there is the minim gap hole on it, gas can diffuse in the described gaseous interchange pipe 21, but other materials such as moisture can't pass through, therefore nutrient solution can carry out gaseous interchange to guarantee the required gas of cell growth with the external world in gaseous interchange pipe 21, and cell is grown in the culture tube 23 that the proper growth condition is arranged.This apparatus structure is simple, can carry out cell cultures efficiently.Described gaseous interchange pipe 21 is the silicon sulfide sebific duct, the silicon sulfide sebific duct has guaranteed that not only nutrient solution and ambient atmos can carry out enough exchanges, itself also has simultaneously good bending property, like this can curved arrangement gaseous interchange pipe 21 under the very long situation of gaseous interchange pipe 21 length, thus make that the volume of whole device is comparatively compact.Described main circulator 20 is the finger peristaltic pump, adopts the finger peristaltic pump can stop to introduce the risk of introduced contaminants so that nutrient solution discord recycle pump directly contacts as main circulator 20; Finger peristaltic pump valveless and sealing member, easy to maintenance.Described culture tube 23 is the tubular fibre culture tube, the fiber of big bundle is loaded into formation tubular fibre culture tube in the columniform shell, cell is placed in the fiber outgrowth, and cell culture fluid can constantly circulate to provide the required nutrition of cell and gas in fiber.Can provide best growing environment for specific cell by the aperture of selecting fiber.The tubular fibre culture tube can allow in less space cell to be carried out highdensity cultivation, makes the small and exquisite practicality of cell cultures plumbing installation like this.
Described thrust-augmenting nozzle comprises first thrust-augmenting nozzle 11 that connects described main circulator 20 and described gaseous interchange pipe 21, is connected second thrust-augmenting nozzle 12 of described gaseous interchange pipe 21 and described culture tube 23, the 4th thrust-augmenting nozzle 14 that is connected the 3rd thrust-augmenting nozzle 13 of described culture tube 23 and described nutrient solution master library 19 and is connected described nutrient solution master library 19 and described main circulator 20, and transparent test container 22 is arranged on the 3rd thrust-augmenting nozzle 13 in the present embodiment.Certainly, described transparent test container 22 also can be arranged on described first thrust-augmenting nozzle 11, second thrust-augmenting nozzle 12 and/or the 4th thrust-augmenting nozzle 14, install the potential of hydrogen situation that transparent test container 22 can be observed nutrient solution intuitively in whole nutrient solution loop additional, the nutrient solution that is not suitable for too greatly in affirmation nutrient solution acidity in time more renewing under the situation of cell growth is grown sustainedly and stably to guarantee cell.
As shown in Figure 2, a kind of cell automatization culture systems that the utility model can be applied thereon, comprise near the sensor 30 that is arranged on the described transparent test container 22, also comprise the liquid-adding device 40 that is connected with described first thrust-augmenting nozzle 11 by the 5th thrust-augmenting nozzle 15 and pass through the waste liquid recovery apparatus 50 that the 6th thrust-augmenting nozzle 16 is connected with described the 3rd thrust-augmenting nozzle 13.This structure can be monitored the potential of hydrogen of the nutrient solution in the cell cultures plumbing installation by sensor 30 in real time, when the acidity that monitors nutrient solution is not suitable for the cell growth too greatly, can start waste liquid recovery apparatus 50 and reclaim nutrient solution, start liquid-adding device 40 after this new nutrient solution is joined in the cell cultures pipeline, thereby growth provides required various nutrients to cell constantly.Described liquid-adding device 40 comprises to be treated liquid feeding thesaurus 41, treats liquid feeding weight measurement instrument 42 and fluid filling pump 43, fluid filling pump 43 startups pump into nutrient solution in the nutrient solution master library 19 from treat liquid feeding thesaurus 41 after receiving the liquid feeding signal, treat whether liquid feeding weight measurement instrument 42 can be measured whole liquid feeding process in real time normal, when liquid feeding is undesired, can report to the police so that the staff can check and remove barriers immediately, guarantee the cell normal growth thereby finish the liquid feeding process.Described waste liquid recovery apparatus 50 comprises recovery liquid thesaurus 51, reclaims liquid weight measurement instrument 52 and liquid collecting pump 53, and whether the weight that reclaims 51 li waste liquids of the first weighing recovery liquid thesaurus of liquid weight measurement instrument 52 meetings before effluent discharge has enough spaces to hold with affirmation is treated effluent discharge.If enough spaces are arranged, waste liquid is discharged to and reclaims in the liquid thesaurus 51, if there are not enough spaces, system can report to the police, treat manually to vacate effluent discharge again behind enough spaces, this has guaranteed that total system can move constantly automatically, has improved the culture efficiency of cell.Described the 6th thrust-augmenting nozzle 16 is connected with the B end of described culture tube 23 by the 7th thrust-augmenting nozzle 17, described the 5th thrust-augmenting nozzle 15 is connected with the A end of described culture tube 23 by the 8th thrust-augmenting nozzle 18, be provided with opening-closing valve at described second thrust-augmenting nozzle 12 near a side of described culture tubes 23, also be provided with opening-closing valve at described the 3rd thrust-augmenting nozzle 13 near a side of described culture tubes 23.This structure can be cleaned culture tube 23 more for a long time automatically in the unfavorable chemical substance that culture tube 23 produces, first opening-closing valve and second switch valve are closed simultaneously during cleaning, reclaim liquid thesaurus 51 from treating that nutrient solution in the liquid feeding thesaurus 41 washed to enter into behind the culture tube 23, thereby finish the cleaning to culture tube 23.Culture tube 23 is carried out in good time cleaning can guarantee that cell has comparatively suitable growing environment, guarantees the cell normal growth.Described sensor 30 comprises photographing module 31 and the light source 32 that places described transparent test container 22 both sides, detrimentally affect when light source 32 and photographing module 31 opposed structures have avoided ambient light to photographing module 31 captures, the potential of hydrogen that makes sensor 30 can react nutrient solution in the transparent test container 22 more truly changes, the signal that control center obtains according to sensor 30 controls whether change nutrient solution, the sensor 30 of this structure can take full advantage of nutrient solution, when nutrient solution can't provide nutrient for cell, change, can not waste nutrient solution.
Described second thrust-augmenting nozzle 12, the 8th thrust-augmenting nozzle 18, the 7th thrust-augmenting nozzle 17 and the 3rd thrust-augmenting nozzle 13 are provided with stopple coupon, stopple coupon can only be arranged on any thrust-augmenting nozzle in four thrust-augmenting nozzles, also can be arranged in four thrust-augmenting nozzles on any two thrust-augmenting nozzles, on any three thrust-augmenting nozzles and on whole four thrust-augmenting nozzles.Stopple coupon handled easily personnel carry out real-time analysis and monitoring to the nutrient solution in the thrust-augmenting nozzle, can grow in the growing environment of the best to guarantee cell.Described main circulator 20, sensor 30, liquid-adding device 40, waste liquid recovery apparatus 50 and opening-closing valve all electrically connect with the master control computer.The master control computer expert crosses control software each parts is controlled, and to realize the automatic operation of described cell automatization culture systems, has reduced human input, has improved culture efficiency.
As shown in Figure 3, a kind of liquid color monitoring device comprises transparent test container 22, also comprises near be arranged on the described transparent test container 22 sensor 30 and and the processing module that electrically connects of described sensor 30.Described sensor 30 comprises that pick-up lens is towards the photographing module 31 of described transparent test container 22, described sensor 30 also comprises near the light source 32 that is arranged on the described transparent test container 22, and described light source 32 and described photographing module 31 are oppositely arranged on the both sides of described transparent test container 22.Side at described light source 32 close described transparent test containers 22 also is provided with diverging lens 321, and the camera lens of described photographing module 31 is high-resolution CMOS camera.Described transparent test container 22 is transparent platinum solidification of silicon sebific duct.The external diameter of described transparent platinum solidification of silicon sebific duct is more than or equal to 3 millimeters, and the thickness of described transparent platinum solidification of silicon sebific duct tube wall is no more than 25% of described transparent platinum solidification of silicon sebific duct external diameter diameter.Described transparent test container 22 and described sensor 30 are installed in the lucifuge case 33.
As shown in Figure 4, a kind of and well-behaved utility model cooperating and can be applied in the cell cultures plumbing installation jointly or cell automatization culture systems on a kind of culture tube transporter embodiment 1: comprise the transshipment acitivity body 61 that is coated on the culture tube 23, also comprise the temperature control module 62 and the occluding device 63 that are arranged on the described transshipment acitivity body, described culture tube 23 is provided with entrance and exit, described entrance is connected with second thrust-augmenting nozzle 12, and described outlet is connected with the 3rd thrust-augmenting nozzle 13.Temperature control module 62 is started working before this culture tube 23 of transhipment, and the temperature of culture tube 23 is reduced in certain temperature range.Cell in the culture tube 23 can be not frozen in this temperature range, and the metabolic activities of these cells reduces greatly simultaneously.So just can guarantee in the regular hour scope, under the situation that nutrition and oxygen are not provided to the cell in these culture tubes 23, can be transported to medical institutions to them safely.Occluding device 63 is in order to culture tube 23 with cultivate piping system and separate rapidly, consuming time when having saved separation and Culture pipe 23 and having cultivated piping system.
Described occluding device 63 comprises the hermetically-sealed connector 70 that is arranged on described second thrust-augmenting nozzle 12 and described the 3rd thrust-augmenting nozzle 13, and described hermetically-sealed connector 70 comprises the twistlock piston.The hermetically-sealed connector 70 that has the twistlock piston can guarantee the stopping property of whole cultivation piping system when being connected with the cultivation piping system, make the nutrient solution of piping system inside can not be subjected to the influence of external environment, thereby the cell in the assurance culture tube 23 can be grown normally.When separation and Culture pipe 23 and cultivation piping system, can promptly both be separated again, and it is isolated to guarantee also to be in nutrient solution and the external environment of cultivating in the piping system, at this moment culture tube to be cultivated 23 can be coupled together by hermetically-sealed connector and cultivation piping system, so just can utilize the nutrient solution of cultivating piping system and cultivating in the piping system to carry out cell cultures again, stop to cultivate the waste of device and nutrient solution.
Described temperature control module 62 comprises water cooler, well heater, temperature sensor and temperature regulator.After configuring temperature range, each device in the temperature control module 62 will remain in the range of set temperature to guarantee temperature by cooperating, thereby guarantee that cell has lower metabolic activity in transport process, make cell to be transported to medical institutions safely.Described transshipment acitivity body 61 comprises mechanism body C portion 611 and the mechanism body D portion 612 that can be fastened togather, and a side of described mechanism body C portion 611 and mechanism body D portion 612 is connected through the hinge and is in the same place.This structure makes transshipment acitivity smaller and more exquisite, convenient carrying back and forth when transshipment acitivity is reused.
As shown in Figure 5, a kind of and well-behaved utility model cooperating and can be applied in the cell cultures plumbing installation jointly or cell automatization culture systems on a kind of culture tube transporter embodiment 2: described culture tube 23 is provided with the entrance and exit that is connected respectively with thrust-augmenting nozzle, in the arranged outside of described thrust-augmenting nozzle heating unit 631 is arranged.Described heating unit 631 comprises the heat block 632 that is arranged on described thrust-augmenting nozzle outside surface both sides.Described heat block 632 can move towards the direction near the axis of described thrust-augmenting nozzle.Namely when in second thrust-augmenting nozzle, 12 outside surface both sides heat block 632 being set, described heat block 632 can move towards the direction near second thrust-augmenting nozzle, 12 axis; When in the 3rd thrust-augmenting nozzle 13 outside surface both sides heat block 632 being set, described heat block 632 can move towards the direction near the 3rd thrust-augmenting nozzle 13 axis; When simultaneously when second thrust-augmenting nozzle, 12 outside surface both sides and the 3rd thrust-augmenting nozzle 13 outside surface both sides arrange heat block 632, the heat block 632 that is arranged on second thrust-augmenting nozzle, 12 outside surface both sides can move towards the direction near second thrust-augmenting nozzle, 12 axis, and the heat block 632 that is arranged on the 3rd thrust-augmenting nozzle 13 outside surface both sides can move towards the direction near the 3rd thrust-augmenting nozzle 13 axis.When the cell of transhipment in the culture tube 23, heat block 632 clamps thrust-augmenting nozzles, and thrust-augmenting nozzle is because being heated and the effect of clamping force is sealed, thereby culture tube 23 and the nutrient solution cultivated in the piping system are kept apart, thereby prepares for transporting culture tube 23.This occluding device 63 is simple in structure, with low cost.Further, described occluding device 63 can also comprise the clamp mechanism that is arranged on described thrust-augmenting nozzle outside surface both sides.So just can with stepping up mechanism conduit be clamped earlier, with the nutrient solution blocking-up in culture tube 23 and the cultivation piping system, heating unit 631 seals pipeline then.Can avoid so directly causing culture-liquid temp to rise to the pipeline heated sealant, temperature control module 62 that need to have stopped to trigger culture tube 23 is lowered the temperature and the energy dissipation that causes.
Must be pointed out that some indefinitenesses that above-described embodiment is just made the utility model illustrate.But person of skill in the art will appreciate that, do not departing under aim of the present utility model and the scope that can make modification to the utility model, replace and change, these modifications, replacement and change still belong to protection domain of the present utility model.
Claims (10)
1. a liquid color monitoring device comprises transparent test container (22), it is characterized in that: also comprise the processing module that is arranged near the sensor (30) of described transparent test container (22) and electrically connects with described sensor (30).
2. a kind of liquid color monitoring device according to claim 1, it is characterized in that: described sensor (30) comprises that pick-up lens is towards the photographing module (31) of described transparent test container (22).
3. a kind of liquid color monitoring device according to claim 2 is characterized in that: described sensor (30) also comprises near the light source (32) that is arranged on the described transparent test container (22).
4. a kind of liquid color monitoring device according to claim 3, it is characterized in that: described light source (32) and described photographing module (31) are oppositely arranged on the both sides of described transparent test container (22).
5. a kind of liquid color monitoring device according to claim 4 is characterized in that: also be provided with diverging lens (321) at described light source (32) near a side of described transparent test container (22).
6. a kind of liquid color monitoring device according to claim 5, it is characterized in that: the camera lens of described photographing module (31) is high-resolution CMOS camera.
7. according to the described a kind of liquid color monitoring device of any one claim in the claim 1 to 6, it is characterized in that: described transparent test container (22) is transparent platinum solidification of silicon sebific duct.
8. a kind of liquid color monitoring device according to claim 7, it is characterized in that: the external diameter of described transparent platinum solidification of silicon sebific duct is more than or equal to 3 millimeters.
9. a kind of liquid color monitoring device according to claim 7, it is characterized in that: the thickness of described transparent platinum solidification of silicon sebific duct tube wall is no more than 25% of described transparent platinum solidification of silicon sebific duct external diameter diameter.
10. according to the described a kind of liquid color monitoring device of any one claim in the claim 1 to 6, it is characterized in that: described transparent test container (22) and described sensor (30) are installed in the lucifuge case (33).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220530901 CN203048942U (en) | 2012-10-17 | 2012-10-17 | Device for monitoring color of liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220530901 CN203048942U (en) | 2012-10-17 | 2012-10-17 | Device for monitoring color of liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203048942U true CN203048942U (en) | 2013-07-10 |
Family
ID=48731919
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220530901 Expired - Lifetime CN203048942U (en) | 2012-10-17 | 2012-10-17 | Device for monitoring color of liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203048942U (en) |
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2012
- 2012-10-17 CN CN 201220530901 patent/CN203048942U/en not_active Expired - Lifetime
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| C14 | Grant of patent or utility model | ||
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| CX01 | Expiry of patent term |
Granted publication date: 20130710 |