CN203025054U - Cell shearing strength measuring device - Google Patents
Cell shearing strength measuring device Download PDFInfo
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- CN203025054U CN203025054U CN 201320027812 CN201320027812U CN203025054U CN 203025054 U CN203025054 U CN 203025054U CN 201320027812 CN201320027812 CN 201320027812 CN 201320027812 U CN201320027812 U CN 201320027812U CN 203025054 U CN203025054 U CN 203025054U
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- cell
- shearing strength
- micrometric displacement
- negative pressure
- cell shearing
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Abstract
The utility model relates to a cell shearing strength measuring device which solves the problem that at present, few cell shearing strength measuring methods are provided, the cost is high, the measurement is difficult and the technology is limited. The cell shearing strength measuring device comprises a spiral micro-displacement driving element, a micro-displacement push plate, a fixed support, a cell shearing strength measuring unit, a negative pressure connecting pipe, a negative pressure adjusting switch, a negative pressure container, a transmission guide rod and a micro-displacement guide rail. Inflowing cell suspensions are arranged into a single row through a vacuum aspiration manner and are fixed at a measuring part, and the cell shearing stress at the tail end of the single-row cell suspension is measured by changing the displacement generated by a cantilever beam. The cell shearing strength measuring device is used for determining the shearing strength of detected cells by adopting an engineering science method and is provided with corresponding apparatuses; and the cell shearing strength measuring device is simple, effective, easy to operate and low in cost, reduces the threshold of cell biomechanics research and is convenient for measuring the cell shearing strength.
Description
Technical field
The utility model belongs to biomedical engineering field, relates to a kind of cell shearing strength meter.
Background technology
Along with developing rapidly of cell biomechanics, structure biology, the Quantitative study of cell microcosmic function and characteristic strengthens day by day, the measuring method of cell shearing stress is also constantly developing and is enriching, but on the whole, present measuring method is fewer, and exist costly, measure the problems such as difficulty and technology limitation.Means of micropipette aspiration technique is applied to mechanocytobiology research in early days, but be confined to flux low, at every turn can only realize single to cell detection, and single longer to test period owing to being subjected to, the external restriction that is difficult to keep for a long time the cell function state is difficult to obtain the statistics of large sample; Atomic force microscopy develops on the technique of scan tunnel microscope basis, possesses more wide application space, but be difficult to record (＜101pN) the interaction, and protein purification is relative loaded down with trivial details with the process that functionalization characterizes of intermolecular low bond strength; It is to complete manipulation function with a kind of on-mechanical way of contact that the light tweezer is controlled technology, but it is not suitable for the large molecular system of action intensity, and to responsive to temperature, is subject to the impact of carrier Brownian movement; Parallel-flow chamber technology is a kind of up-to-date experimental technique near physiological conditions, and intermolecular two-dimentional reaction power theory and model are not perfect under shear flow, is difficult to quantize all sidedly the information of intermolecular combination and Dissociation.
Summary of the invention
The purpose of this utility model is to provide a kind of simple and feasible cell shearing intensity detecting device, this device can be to the cell suspension that flows into this device the mode by vacuum suction be arranged in it single-row and be fixed on the position of measurement, the displacement that produces by changing semi-girder, cell shearing stress to single-row cell suspension end is measured, wherein can regulate accordingly the size of shear stress, carry out the measurement of cell shearing stress under the Parameter Conditions of the best, draw optimum result.This device has been under totally enclosed environment, can keep the good culture environment of cell, avoids ambient temperature, humidity, potential of hydrogen and bacterium factors on the impact of measurement data.
The utility model comprises spiral micrometric displacement actuated element, micrometric displacement push pedal, fixed support, cell shearing ionization meter unit, negative pressure connecting pipe, negative pressure by-pass cock, negative-pressure container, transmission guide rod and micrometric displacement guide rail.
Be provided with the micrometric displacement guide rail in fixed support, micrometric displacement push pedal and micrometric displacement guide rail are slidably matched, and a support bracket fastened end also is provided with spiral micrometric displacement actuated element, and by adjustable screw micrometric displacement actuated element, the micrometric displacement push pedal produces displacement.
The support bracket fastened other end is fixedly installed cell shearing ionization meter unit, and described cell shearing ionization meter unit comprises cell shearing ionization meter unit baseboard, clamping signaling plate, transmission guide rod fixed mount and semi-girder microfilament.
Have the cell suspension runner on cell shearing ionization meter unit baseboard, outlet one side of cell suspension runner is provided with semi-girder microfilament and clamping signaling plate, described semi-girder microfilament is parallel with the cell suspension runner, and the one end is fixed on the transmission guide rod fixed mount; The transmission guide rod fixed mount is connected with the micrometric displacement push pedal by transmission guide rod.
Also have the cell suspension liquid outlet on cell shearing ionization meter unit baseboard, the cell suspension liquid outlet is connected with an end of negative pressure connecting pipe, and the other end of negative pressure connecting pipe connects negative-pressure container, also is provided with the negative pressure by-pass cock on negative-pressure container.
The utility model compared with prior art, the beneficial effect that has is: according to the semi-girder formula of theoretical mechanics, seek the transformation relation of shear stress and displacement by conversion, adopt the method for engineering science determine to detect the method for cell shearing intensity and design corresponding apparatus, effectively simple, easy operating has reduced the threshold that cell biomechanics is studied, and has facilitated the measurement of cell shearing stress.
Description of drawings
Fig. 1 is one-piece construction figure of the present utility model;
Fig. 2 is cell shearing ionization meter cellular construction schematic diagram in Fig. 1;
Fig. 3 is the partial enlarged drawing of cell shearing ionization meter unit in Fig. 2;
Fig. 4 is that semi-girder calculates schematic diagram.
Embodiment
The utility model is described in further detail below in conjunction with accompanying drawing.
As shown in Figure 1, the present embodiment comprises that mainly vacuum suction mechanism, cell suspension four parts such as mechanism, cell shearing stress detection mechanism, displacement propulsive mechanism that flow form.
Vacuum suction mechanism comprises: cell suspension liquid outlet 16; Negative pressure connecting pipe 6; Negative pressure by-pass cock 7; Negative-pressure container 8.In the situation that negative pressure by-pass cock 7 is opened, cell suspension is flowed into by cell suspension liquid outlet 16, flows into negative-pressure container 8 through negative pressure connecting pipe 6, negative pressure by-pass cock 7.
The mobile mechanism of cell suspension comprises: cell suspension inlet cover 4; Cell shearing ionization meter unit 5; Cell suspension runner 11.Wherein cell suspension by cell suspension inlet cover 4 inflow cell shearing ionization meter unit, places 5, enters cell suspension runner 11.
As shown in Figures 2 and 3, the cell shearing stress detection mechanism comprises: clamping signaling plate 12; Transmission guide rod fixed mount 13; Semi-girder microfilament 14; Cell shearing ionization meter unit baseboard 15.Cell shearing ionization meter unit baseboard 15 is the substrate of measuring unit, play the effect of fixing each device, clamping signaling plate 12, transmission guide rod fixed mount 13 and semi-girder microfilament 14 are fixed together, can move under the effect of displacement propulsion plant, when running into cell and stopping, the transmission guide rod fixed mount separates with the semi-girder microfilament, measures corresponding displacement.
The displacement propulsive mechanism comprises: spiral micrometric displacement actuated element 1; Micrometric displacement push pedal 2; Fixed support 3; Transmission guide rod 9; Micrometric displacement guide rail 10.Fixed support 3 be mainly used to fix other all parts and and are connected part connection, micrometric displacement push pedal 2 is placed on micrometric displacement guide rail 10, by adjustable screw micrometric displacement actuated element 1, micrometric displacement push pedal 2 will produce displacement, promote transmission guide rod 9 thereupon and produce displacement, promote the motion of cell shearing stress detection mechanism, carry out corresponding detection.
The method of cell shearing ionization meter specifically comprises the following steps:
at first guarantee clamping signaling plate 12, transmission guide rod fixed mount 13, semi-girder microfilament 14 is in the position of cell suspension runner 11 position that clamping signaling plate has blocked the cell suspension runner exit, cell shearing strength meter 5 injects with cell suspension in 4 places in the cell suspension inlet cover, and cell suspension will flow into cell suspension runner 11, and forms the row stream of cells that individual cells is arranged in runner, open negative pressure by-pass cock 7, carrying out negative pressure regulates, cell suspension in cell suspension runner 11 will flow to cell suspension liquid outlet place, but the existence due to clamping signaling plate 12, cell has been blocked in the intersection of cell suspension runner 11 with clamping signaling plate 12, this moment, adjustable screw micrometric displacement actuated element 1, micrometric displacement push pedal 2 will be moved on micrometric displacement guide rail 10, and drive the motion of drive rail 9 thereupon, and be connected with transmission guide rod fixed mount 13 due to drive rail 9, so drive rail 9 can drive the motion of transmission guide rod fixed mount 13, then drive clamping signaling plate 12 and 14 motions of semi-girder microfilament, when the stopping of cell of end of suspension trichome at cell suspension runner 11 places encountered in semi-girder microfilament 14 motion, semi-girder microfilament 14 will be distorted and produce displacement, adjusting along with spiral micrometric displacement actuated element 1, the suffered shear stress of cell constantly increases, can reach at last and bear the limit, this moment, cell was cut off, correspondence is cut off the maximum displacement of cell and is measured by the worm drive element, carry out the calculating of corresponding cell shearing stress according to the semi-girder formula.After completing measurement, this cell will flow in negative-pressure containers 8 by the cell suspension liquid outlet 16 negative pressure connecting pipe 6, negative pressure by-pass cock 7 of flowing through.Can carry out the measurement of the shear stress of next cell this moment.After testing the shear stress of a series of cells, we just can carry out corresponding data to measurement result and process and calculate.
As shown in Figure 4, shear stress Principle and method of measurement:
Classical formulas according to semi-girder:
The expression formula that gets stress and end section corner by conversion is:
In formula:
The length of expression beam;
The length travel of expression B end;
Expression B holds to get stressed size;
The elastic modulus of expression semi-girder;
The moment of inertia of expression semi-girder;
Expression B end section corner.When material and cross sectional shape one timing of beam, the B end is stressed has just formed corresponding relation with the radial degree of B end; Like this, by measuring the radial degree of B end, just can know the stressed size of B end, control stressed range by the length that changes beam.
Claims (1)
1. cell shearing strength meter, comprise spiral micrometric displacement actuated element, micrometric displacement push pedal, fixed support, cell shearing ionization meter unit, negative pressure connecting pipe, negative pressure by-pass cock, negative-pressure container, transmission guide rod and micrometric displacement guide rail, it is characterized in that:
Be provided with the micrometric displacement guide rail in fixed support, micrometric displacement push pedal and micrometric displacement guide rail are slidably matched, and a support bracket fastened end also is provided with spiral micrometric displacement actuated element, and by adjustable screw micrometric displacement actuated element, the micrometric displacement push pedal produces displacement;
The support bracket fastened other end is fixedly installed cell shearing ionization meter unit, and described cell shearing ionization meter unit comprises cell shearing ionization meter unit baseboard, clamping signaling plate, transmission guide rod fixed mount and semi-girder microfilament;
Have the cell suspension runner on cell shearing ionization meter unit baseboard, outlet one side of cell suspension runner is provided with semi-girder microfilament and clamping signaling plate, described semi-girder microfilament is parallel with the cell suspension runner, and the one end is fixed on the transmission guide rod fixed mount; The transmission guide rod fixed mount is connected with the micrometric displacement push pedal by transmission guide rod;
Also have the cell suspension liquid outlet on cell shearing ionization meter unit baseboard, the cell suspension liquid outlet is connected with an end of negative pressure connecting pipe, and the other end of negative pressure connecting pipe connects negative-pressure container, also is provided with the negative pressure by-pass cock on negative-pressure container.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320027812 CN203025054U (en) | 2013-01-17 | 2013-01-17 | Cell shearing strength measuring device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320027812 CN203025054U (en) | 2013-01-17 | 2013-01-17 | Cell shearing strength measuring device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203025054U true CN203025054U (en) | 2013-06-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320027812 Expired - Fee Related CN203025054U (en) | 2013-01-17 | 2013-01-17 | Cell shearing strength measuring device |
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| Country | Link |
|---|---|
| CN (1) | CN203025054U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103087908A (en) * | 2013-01-17 | 2013-05-08 | 杭州电子科技大学 | Method and device for measuring shear strength of cells |
-
2013
- 2013-01-17 CN CN 201320027812 patent/CN203025054U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103087908A (en) * | 2013-01-17 | 2013-05-08 | 杭州电子科技大学 | Method and device for measuring shear strength of cells |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130626 Termination date: 20150117 |
|
| EXPY | Termination of patent right or utility model |