CN202849380U - Probiotics liquid fermentation device - Google Patents

Probiotics liquid fermentation device Download PDF

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Publication number
CN202849380U
CN202849380U CN 201220516975 CN201220516975U CN202849380U CN 202849380 U CN202849380 U CN 202849380U CN 201220516975 CN201220516975 CN 201220516975 CN 201220516975 U CN201220516975 U CN 201220516975U CN 202849380 U CN202849380 U CN 202849380U
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China
Prior art keywords
probiotic bacterium
bacterium liquid
distillation apparatus
plastic tank
utility
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Expired - Fee Related
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CN 201220516975
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Chinese (zh)
Inventor
徐春厚
刘颖
谢为天
李淑芳
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Guangdong Ocean University
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Guangdong Ocean University
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Priority to CN 201220516975 priority Critical patent/CN202849380U/en
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Publication of CN202849380U publication Critical patent/CN202849380U/en
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Abstract

The utility model discloses a probiotics liquid fermentation device. The fermentation device comprises a plastic barrel (1) with a cover, and a water distiller (2), wherein distilled water generated by the water distiller (2) flows into the plastic barrel (1) in which a constant temperature heater (3) and a stirrer (4) are arranged. The fermentation device is simple in structure, easy to operate, low in cost, less in investment and suitable for being used by small-sized prebiotics production enterprises, livestock farms and farmers.

Description

Probiotic bacterium liquid fermenting device
Technical field
The utility model belongs to the technical field of biotechnology, specifically refers to a kind of probiotic bacterium liquid fermenting device.
Background technology
At present, along with the development of aquaculture and feed industry, the application of probiotics is more and more universal, has become the ideal substitute of feeding antibiotic.The at present production of probiotics uses the stainless steel fermentor tank to carry out liquid fermenting more, this method technique is complicated, and need to buy 2-3 fermentor tank, the price of the energy temperature control of a 200L and the fermentor tank of stirring is about 6000 ~ 8000 yuan, cost is higher, investment is larger, for small-sized probiotics manufacturing enterprise, plant and raiser, adopt existing probiotic bacterium liquid fermentation process and device to produce probiotics and not too gear to actual circumstances, also make production and the use cost increase of probiotics.
Above-mentioned discussion content purpose is to introduce to the reader all respects of technology that may be relevant with all respects of the present utility model that below will be described and/or advocate, believe that this discussion content helps to provide background information for the reader, to be conducive to understanding better all respects of the present utility model, therefore, should be appreciated that it is to read these discussions with this angle, rather than admit prior art.
The utility model content
The purpose of this utility model is to avoid deficiency of the prior art and a kind of simple and easy to do probiotic bacterium liquid fermenting device is provided, to solve probiotics production and to buy the problem that the fermentation unit cost is high and investment is large.
The purpose of this utility model is achieved through the following technical solutions:
A kind of probiotic bacterium liquid fermenting device is provided, comprises plastic tank with cover, water distillation apparatus, the distilled water that described water distillation apparatus produces flows in the described plastic tank, is provided with constent temperature heater and agitator in the described plastic tank.
Wherein, the capacity of described plastic tank is 75 ~ 100L.
Wherein, described water distillation apparatus is the stainless steel water distillation apparatus, aquifer yield 5 ~ 10L/h, power 4.5 ~ 7.5KW.
Wherein, described constent temperature heater is the stainless steel Shui nationality heating rod with temperature controller, power 300 ~ 500W, 20 ℃ ~ 34 ℃ of temperature control scopes.
Wherein, described agitator is that fish jar is made unrestrained pump, power 7 ~ 10W.
The beneficial effects of the utility model: the utility model is simple in structure, easy to operate, cost is low, less investment, is suitable for small-sized probiotics manufacturing enterprise, plant and raiser and uses.
Description of drawings
The utility model is described in further detail to utilize accompanying drawing, but the embodiment in the accompanying drawing does not consist of any restriction of the present utility model, for those of ordinary skill in the art, under the prerequisite of not paying creative work, can also obtain according to the following drawings other accompanying drawing.
Fig. 1 is the structural representation of probiotic bacterium liquid fermenting device.
Embodiment
In order to make those skilled in the art understand better the technical solution of the utility model, below in conjunction with the drawings and specific embodiments the utility model is described in further detail, need to prove, in the situation that do not conflict, the application's embodiment and the feature among the embodiment can make up mutually.
Core of the present utility model is to provide a kind of probiotic bacterium liquid fermenting device, to solve probiotics production and to buy the problem that the fermentation unit cost is high and investment is large.
As shown in Figure 1, probiotic bacterium liquid fermenting device described in the utility model comprises plastic tank with cover 1, water distillation apparatus 2.The capacity of described plastic tank 1 is 75 ~ 100L.Described water distillation apparatus 2 is the stainless steel water distillation apparatus, aquifer yield 5 ~ 10L/h, and power 4.5 ~ 7.5KW, the distilled water that described water distillation apparatus 2 produces flows in the described plastic tank 1, is provided with constent temperature heater 3 and agitator 4 in the described plastic tank 1.Described constent temperature heater 3 is the stainless steel Shui nationality heating rod with temperature controller, power 300 ~ 500W, 20 ℃ ~ 34 ℃ of temperature control scopes.Described agitator 4 is made unrestrained pump for fish jar, power 7 ~ 10W.
The below is described in detail the concrete application of adopting aforesaid probiotic bacterium liquid fermenting device for different probiotic bacteriums.
The preparation of subtilis probiotic bacterium liquid, step is as follows:
(1) prepare fermentation unit: place the 7W fish jar in the bottom of 75L plastic tank and make unrestrained pump, place 300W stainless steel Shui nationality heating rods (temperature transfers to 34 ℃) at the sidewall of bucket, inject the distilled water 65L that is flowed out by water distillation apparatus in bucket, water temperature is 81 ℃;
(2) preparation liquid fermentation medium: with peptone, yeast extract paste, glucose, NaCl, K 2HPO 4And MgSO 47H 20 grade joins in the Stainless Steel Kettle that 6L distilled water is housed in proportion, and electromagnetic oven heating is boiled approximately 25min, pours in the barreled distilled water of being down to room temperature after each composition dissolves fully, is liquid fermentation medium;
(3) inoculation probiotic bacterium: carry out activation culture with subtilis as probiotic strain, subtilis activation culture thing 4L is inoculated in the liquid fermentation medium, vaccination ways can be single strain inoculation or the combined inoculation of many bacterial strains;
(4) probiotics fermention: connect the power supply that fish jar is made unrestrained pump and stainless steel Shui nationality heating rod, can see the ripple glaze of media surface, cover tightly plastic drum lid, continuing fermentation 48h under 34 ℃ temperature obtains 75L subtilis probiotic bacterium liquid.
Production cost calculates: calculate by every barreled 75L liquid fermentation medium and inoculum fermented 48h, production cost is: distilled water power consumption processed is 1.5 degree, electromagnetic oven dissolving substratum power consumption is 1 degree, the heating rod power consumption is 11 degree, making unrestrained pump power consumption is 0.4 degree, adding up to power consumption is 14 degree, and the electricity charge are about 14 yuan; The cost of 75L liquid fermentation medium is about 110 yuan; Be 1 month, produce probiotics fermention liquid 5000L and 3000 yuan of calculating of personnel's wage per month by the fermentation unit usage period, the depreciable cost of every liter of probiotics and personnel's wage are about 0.9 yuan; The production cost of subtilis probiotic bacterium liquid is about 2.6 yuan/liter.
The preparation of milk-acid bacteria, genus bacillus and yeast compound probiotic liquid, step is as follows:
(1) prepares fermentation unit: prepare 8 75L plastic tanks, 8 10W fish jars are made unrestrained pump and 8 500W stainless steel Shui nationality heating rods, place fish jar in the bottom of 75L plastic tank and make unrestrained pump, sidewall at bucket is placed stainless steel Shui nationality heating rods (temperature transfers to 34 ℃), inject the distilled water 65L that is flowed out by water distillation apparatus in bucket, water temperature is 80 ℃;
(2) preparation liquid fermentation medium: with peptone, yeast extract paste, glucose, NaCl, K 2HPO 4And MgSO 47H 20 grade joins in the Stainless Steel Kettle that 6L distilled water is housed in proportion, and electromagnetic oven heating is boiled approximately 25min, pours in the barreled distilled water of being down to room temperature after each composition dissolves fully, is liquid fermentation medium;
(3) inoculation probiotic bacterium: carry out activation culture as probiotic strain with enterococcus faecalis, subtilis and cereuisiae fermentum, enterococcus faecalis activation culture thing 1.5L, subtilis activation culture thing 3L and cereuisiae fermentum activation culture thing 1L are inoculated in the liquid fermentation medium, and vaccination ways can be single strain inoculation or the combined inoculation of many bacterial strains;
(4) probiotics fermention: connect the power supply that fish jar is made unrestrained pump and stainless steel Shui nationality heating rod, can see the ripple glaze of media surface, cover tightly plastic drum lid, continuing fermentation 48h under 34 ℃ temperature obtains 600L compound probiotic liquid.
Production cost calculates: press 600L liquid fermentation medium and inoculum fermented 48h and calculate, production cost is: distilled water power consumption processed is 12 degree, electromagnetic oven dissolving substratum power consumption is 10 degree, the heating rod power consumption is 128 degree, make unrestrained pump power consumption and be about 4 degree, add up to power consumption to be about 154 degree, the electricity charge are about 154 yuan; The cost of 600L liquid fermentation medium is about 880 yuan; Be 1 month, produce probiotics fermention liquid 5000L and 3000 yuan of calculating of personnel's wage per month by the fermentation unit usage period, the depreciable cost of every liter of probiotics and personnel's wage are about 0.9 yuan; The production cost of compound probiotic liquid is about 2.7 yuan/liter.
In conjunction with top concrete application, the beneficial effects of the utility model are:
The method selects electromagnetic oven to heat, and the medium component dissolution time is short, and has reached the purpose of sterilization; Directly inject the distilled water that water distillation apparatus flows out in the plastic tank, water temperature can reach more than 78 ℃, has guaranteed the sterilization of plastic tank and preparation substratum water;
Because the capacity of plastic tank is little, it is short that distilled water is down to the room temperature time in the bucket, can pour into after medium component dissolves fully in the barreled distilled water of being down to room temperature, the liquid fermentation medium temperature of this moment is no more than 37 ℃, can inoculate immediately probiotic bacterium and carry out fermentation culture;
The fermentation unit of the method is simple, only needs to make unrestrained pump and water distillation apparatus from market purchase plastic tank, stainless steel Shui nationality heating rod, fish jar, fits together, and can satisfy the condition of the needed constant temperature of probiotic's culture and ventilation, and can guarantee sterile state;
Buy the fermentation unit less investment, 60 ~ 80 yuan of 75L plastic tanks, 40 ~ 50 yuan of stainless steel Shui nationality heating rods, fish jar are made 30 ~ 40 yuan of unrestrained pumps, 350 ~ 500 yuan of water distillation apparatuss; By 8 plastic tanks, heating rods with make unrestrained pump and 1 water distillation apparatus is one group, invest altogether approximately 1400 ~ 1700 yuan, can produce the probiotics fermention liquid of 600L, and fermentation unit can be repeatedly used at every turn;
Set forth a lot of details so that fully understand the utility model in the top description, still, the utility model can also adopt other to be different from other modes described here and implement, and therefore, can not be interpreted as the restriction to the utility model protection domain.
In a word; although the utility model has exemplified above-mentioned preferred implementation; but should illustrate; although those skilled in the art can carry out various variations and remodeling; unless such variation and remodeling have departed from scope of the present utility model, otherwise all should be included in the protection domain of the present utility model.

Claims (5)

1. probiotic bacterium liquid fermenting device, it is characterized in that, comprise plastic tank with cover (1), water distillation apparatus (2), the distilled water that described water distillation apparatus (2) produces flows in the described plastic tank (1), is provided with constent temperature heater (3) and agitator (4) in the described plastic tank (1).
2. probiotic bacterium liquid fermenting device according to claim 1, it is characterized in that: the capacity of described plastic tank (1) is 75 ~ 100L.
3. probiotic bacterium liquid fermenting device according to claim 2, it is characterized in that: described water distillation apparatus (2) is the stainless steel water distillation apparatus, aquifer yield 5 ~ 10L/h, power 4.5 ~ 7.5KW.
4. probiotic bacterium liquid fermenting device according to claim 3 is characterized in that: described constent temperature heater (3) is the stainless steel Shui nationality heating rod with temperature controller, power 300 ~ 500W, 20 ℃ ~ 34 ℃ of temperature control scopes.
5. according to claim 1 and 2 or 3 described probiotic bacterium liquid fermenting devices, it is characterized in that: described agitator (4) is made unrestrained pump for fish jar, power 7 ~ 10W.
CN 201220516975 2012-10-10 2012-10-10 Probiotics liquid fermentation device Expired - Fee Related CN202849380U (en)

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CN 201220516975 CN202849380U (en) 2012-10-10 2012-10-10 Probiotics liquid fermentation device

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CN 201220516975 CN202849380U (en) 2012-10-10 2012-10-10 Probiotics liquid fermentation device

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CN202849380U true CN202849380U (en) 2013-04-03

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105331532A (en) * 2015-10-30 2016-02-17 四川蒙特新能源科技有限公司 Anaerobic tank treatment system having wave generating function

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105331532A (en) * 2015-10-30 2016-02-17 四川蒙特新能源科技有限公司 Anaerobic tank treatment system having wave generating function

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Granted publication date: 20130403

Termination date: 20151010

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