CN202837183U - Capillary electrophoresis detection device - Google Patents
Capillary electrophoresis detection device Download PDFInfo
- Publication number
- CN202837183U CN202837183U CN 201220466657 CN201220466657U CN202837183U CN 202837183 U CN202837183 U CN 202837183U CN 201220466657 CN201220466657 CN 201220466657 CN 201220466657 U CN201220466657 U CN 201220466657U CN 202837183 U CN202837183 U CN 202837183U
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- capillary electrophoresis
- light path
- checkout equipment
- light
- equipment according
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Abstract
The utility model relates to a capillary electrophoresis detection device which is provided with a capillary electrophoresis system used for being detected and a cassette. The cassette is provided with a hole channel penetrated through by a capillary. A laser generator and a microscope photographic device are fixedly installed on the cassette. A light emitting path of the laser generator is intersected with a light receiving path of the microscope photographic device to form a light path plane. An axis of the hole channel is perpendicular to the light path plane and a perpendicular foot is located on a point of intersection of the light emitting path and the light receiving path. A data output end of the microscope photographic device is connected with a computer with a display function. The microscope photographic device comprises a microscope lens and an industrial camera. A data output end of the industrial camera adopts a universal serial bus (USB) port and is electrically connected with the computer through the USB port. The capillary electrophoresis detection device has the advantages of being exquisite in structure, low in construction cost, capable of being used in experiments with no need for light path correction, convenient to operate, capable of directly generating image output and visual in observation.
Description
Technical field
The utility model relates to a kind of novel detection device of simplifying the Capillary Electrophoresis data acquisition, is specifically related to a kind of Capillary Electrophoresis checkout equipment.
Background technology
Capillary Electrophoresis (CE), be a class take kapillary as split tunnel, take high-voltage dc as driving force, the liquid phase differential take the multifrequency nature of sample as basis is the few a kind of isolation technics of sample and reagent consumption from technology.
For Capillary Electrophoresis, many detection methods are arranged.Mainly contain laser-induced fluorescence (LIF) (LIF) detection method, can reach the Single Molecule Detection level.
Existing Capillary Electrophoresis data collector-laser-induced fluorescence (LIF) (LIF) detecting device mainly contains laser instrument, detection cell and electrooptical device etc. and partly forms.Wherein, electrooptical device mainly contains photomultiplier (PMT) and CCD (charge-coupled image sensor) etc.Existing Capillary Electrophoresis data collector mainly has the following disadvantages:
1. before each experiment, all need carry out the calibration of laser optical path, elapsed time;
2. photomultiplier can not produce image, observes not directly perceived;
3. need special darkroom, not convenient;
4. need the auxiliary equipment such as vibration isolators;
5. general structure is huge;
6.CCD (charge-coupled image sensor) has imaging function, but expensive, needs low-temperature operation;
7. the device overall cost is high.
8. can't carry.
The utility model content
In order to overcome the defects under the prior art, the purpose of this utility model is to provide a kind of Capillary Electrophoresis pick-up unit, make its have system's exquisiteness, easy to operate, observe the plurality of advantages such as directly perceived, that cost is lower.
The technical solution of the utility model is: a kind of Capillary Electrophoresis checkout equipment, be provided with the capillary electrophoresis system of detected usefulness, be provided with magazine, described magazine is provided with the duct of passing for kapillary, be installed with optical generator and photoelectric conversion device on the described magazine, the reception light light path of the utilizing emitted light light path of described optical generator and described photoelectric conversion device is handed over mutually and is consisted of a light path plane, and the axis in described duct is crossed the utilizing emitted light light path and received the intersection point of light light path.
The axis in described duct is positioned at intersection point, the axis in described duct and the dead in line capillaceous of passing described duct of utilizing emitted light light path and reception light light path perpendicular to described light path plane and intersection point.
The data output end of described photoelectric conversion device can be connected with data processor.
Described optical generator preferably adopts laser generator.
Described photoelectric conversion device adopts micro-photographing apparatus.
Can be provided with the optical filter of filtering clutter on the reception light light path of described micro-photographing apparatus, described optical filter is installed between the axis of described micro-photographing apparatus and described through hole.
Described micro-photographing apparatus comprises microlens and industrial camera, and the data output end of described industrial camera adopts USB interface.
The imageing sensor of described industrial camera preferably adopts large face battle array cmos device.
Described data processor can be for having the computing machine of Presentation Function, and described computing machine is electrically connected with described USB interface.
The beneficial effects of the utility model are:
1. because described laser generator accurately has been installed on the magazine, before the experiment, need not carry out the calibration of laser optical path, save the time;
2. owing on the described magazine described micro-photographing apparatus is installed, has overcome photomultiplier and can not produce image, observe intuitively deficiency;
3. because described magazine has replaced the darkroom, do not need to arrange again special darkroom;
4. because each device firmly fixes and is installed on the magazine, do not need the auxiliary equipment such as vibration isolators;
5. the magazine structure is small and exquisite, has overcome the huge deficiency of general structure;
6. lower owing to described micro-photographing apparatus cost, and can use at normal temperatures, overcome the deficiency that CCD (charge-coupled image sensor) is expensive, need low-temperature operation;
7. compare with existing apparatus, overall cost is lower, is beneficial to popularizing of technology;
8. Capillary Electrophoresis checkout equipment volume is little, and is easy to carry.
Description of drawings
Fig. 1 is the structural representation of a kind of embodiment of the present utility model.
Fig. 2 is the detection light path synoptic diagram of present embodiment.
Embodiment
Referring to Fig. 1 and Fig. 2, a kind of Capillary Electrophoresis checkout equipment, be provided with the capillary electrophoresis system of detected usefulness, be provided with magazine 1, described magazine 1 is provided with the duct 3 of passing for kapillary, described magazine 1 is installed with optical generator 2 and photoelectric conversion device 5, and the reception light light path of the utilizing emitted light light path of described optical generator 2 and photoelectric conversion device 5 is handed over mutually and consisted of a light path plane, and the axis in described duct 3 is crossed the utilizing emitted light light path and received the intersection point of light light path.Preferably, the axis in described duct 3 is positioned at the intersection point of utilizing emitted light light path and reception light light path perpendicular to described light path plane and intersection point.The axis in described duct 3 and the dead in line capillaceous of passing described duct 3.Described magazine 1 has replaced the darkroom, does not need to arrange special darkroom again.Because each device firmly fixes and is installed on the magazine 1, does not need the auxiliary equipment such as vibration isolators.Magazine 1 structure is small and exquisite, has overcome the huge deficiency of general structure.
The data output end of described photoelectric conversion device 5 is connected with data processor 8, can be used as the instrument that data are processed, data show and store.
Described optical generator 2 adopts laser generator, and the described laser generator of accurately installing and fixing need not each data acquisition and all carries out the position adjustment, has saved the time.
Described photoelectric conversion device 5 adopts micro-photographing apparatus, has overcome photomultiplier and can not produce image, observe intuitively deficiency.Because described micro-photographing apparatus cost is lower, and can use at normal temperatures, it is expensive to have overcome CCD (charge-coupled image sensor), needs the deficiency of low-temperature operation.
The reception light light path of described micro-photographing apparatus 5 is provided with the optical filter 4 of filtering clutter, and described optical filter 4 is installed between the axis of described micro-photographing apparatus 5 and described through hole 3, and its central optical axis is perpendicular to described reception light light path.Under Ear Mucosa Treated by He Ne Laser Irradiation was induced, sample sent fluorescence, and the described optical filter of process is received by described micro-photographing apparatus after filtering harmful clutter.
Described micro-photographing apparatus 5 comprises microlens 6 and industrial camera 7, and the data output end of described industrial camera 7 adopts USB interface.
The imageing sensor of described industrial camera 7 adopts large face battle array cmos device, and resolution is high, compares with traditional CCD, and cost is lower, and sharpness is more guaranteed.
Described data processor 8 is for having the computing machine of Presentation Function, can also be the equipment that registering instrument or integraph etc. can record and process collection of illustrative plates.Described computing machine 8 is electrically connected with described USB interface.After described micro-photographing apparatus 5 changes light signal into electric signal, export to described computing machine 8, for described computing machine 8 process in real time, demonstration and storage data.
The utility model with under the prior art one by one independently function element (such as laser generator, optical filter, microlens, industrial camera etc.) be combined in together by the magazine structure, replaced original darkroom pattern, not only dwindled the volume of instrument, easy to carry, compare with existing apparatus, overall cost reduces, be beneficial to popularizing of technology, also because having omitted the calibration of laser optical path, improved efficient, and by directly being connected with apparatus such as computer, cooperate corresponding program to carry out real-time picture control, gather and the data processing, simplified data acquisition and the processing of Capillary Electrophoresis testing result, reach efficient, convenient, economic target.
In concrete use procedure, at first assemble Capillary Electrophoresis, kapillary with low tension groove end passes duct described in this equipment 3 afterwards, until make detected window capillaceous be in utilizing emitted light light path and the intersection point place that receives the light light path on the magazine 1 interior described light path plane, and the adjustment kapillary makes detected window aim at utilizing emitted light light path and reception light light path.Afterwards this equipment is connected with computer by USB interface, opens corresponding program, can begin experiment.
Claims (10)
1. Capillary Electrophoresis checkout equipment, be provided with the capillary electrophoresis system of detected usefulness, it is characterized in that being provided with magazine, described magazine is provided with the duct of passing for kapillary, be installed with optical generator and photoelectric conversion device on the described magazine, the reception light light path of the utilizing emitted light light path of described optical generator and described photoelectric conversion device is handed over mutually and is consisted of a light path plane, and the axis in described duct is crossed the utilizing emitted light light path and received the intersection point of light light path.
2. Capillary Electrophoresis checkout equipment according to claim 1 is characterized in that the axis in described duct is positioned at the intersection point of utilizing emitted light light path and reception light light path perpendicular to described light path plane and intersection point.
3. Capillary Electrophoresis checkout equipment according to claim 1 is characterized in that the data output end of described photoelectric conversion device is connected with data processor.
4. Capillary Electrophoresis checkout equipment according to claim 1 is characterized in that described optical generator adopts laser generator.
5. according to claim 1,2,3 or 4 described Capillary Electrophoresis checkout equipments, it is characterized in that described photoelectric conversion device adopts micro-photographing apparatus.
6. Capillary Electrophoresis checkout equipment according to claim 5 is characterized in that the reception light light path of described micro-photographing apparatus is provided with optical filter.
7. Capillary Electrophoresis checkout equipment according to claim 5 is characterized in that described micro-photographing apparatus comprises microlens and industrial camera, and the data output end of described industrial camera adopts USB interface.
8. Capillary Electrophoresis checkout equipment according to claim 7 is characterized in that the imageing sensor of described industrial camera adopts large face battle array cmos device.
9. the described Capillary Electrophoresis checkout equipment of any one claim according to claim 3-8 is characterized in that described data processor is the computing machine with Presentation Function.
10. Capillary Electrophoresis checkout equipment according to claim 9 is characterized in that described computing machine is electrically connected with described USB interface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220466657 CN202837183U (en) | 2012-09-14 | 2012-09-14 | Capillary electrophoresis detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220466657 CN202837183U (en) | 2012-09-14 | 2012-09-14 | Capillary electrophoresis detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202837183U true CN202837183U (en) | 2013-03-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220466657 Expired - Fee Related CN202837183U (en) | 2012-09-14 | 2012-09-14 | Capillary electrophoresis detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202837183U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104267059A (en) * | 2013-12-16 | 2015-01-07 | 浙江工业大学 | An apparatus and a method of measuring solubility of an inorganic salt in subcritical water by the apparatus |
| CN108388856A (en) * | 2018-02-11 | 2018-08-10 | 天津市吉赫科技有限公司 | The detection device and detection method identified for product casing character and graphic and version |
-
2012
- 2012-09-14 CN CN 201220466657 patent/CN202837183U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104267059A (en) * | 2013-12-16 | 2015-01-07 | 浙江工业大学 | An apparatus and a method of measuring solubility of an inorganic salt in subcritical water by the apparatus |
| CN108388856A (en) * | 2018-02-11 | 2018-08-10 | 天津市吉赫科技有限公司 | The detection device and detection method identified for product casing character and graphic and version |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130327 Termination date: 20140914 |
|
| EXPY | Termination of patent right or utility model |