CN202631476U - Three-electrode device used for enzyme-injection-type glucose biosensor - Google Patents
Three-electrode device used for enzyme-injection-type glucose biosensor Download PDFInfo
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- CN202631476U CN202631476U CN 201220071992 CN201220071992U CN202631476U CN 202631476 U CN202631476 U CN 202631476U CN 201220071992 CN201220071992 CN 201220071992 CN 201220071992 U CN201220071992 U CN 201220071992U CN 202631476 U CN202631476 U CN 202631476U
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Abstract
The utility model discloses a three-electrode device used for an enzyme-injection-type glucose biosensor, and belongs to an electrochemical measurement technology. According to the device, a working electrode, a counter electrode and a reference electrode are fixed in an electrode fixing body, and form a three-electrode system in the electrochemical measurement technology together; a buffer solution and enzyme solution inlet, a buffer solution and solution to be measured inlet and a liquid outlet are formed in the electrode fixing body; the buffer solution and enzyme solution inlet is connected with an enzyme solution quantitative injection device and a buffer solution bottle; the buffer solution and solution to be measured inlet is connected with a solution to be measured quantitative injection device and the buffer solution bottle; and the liquid outlet is connected with a waste liquor bottle. The device is applied to the enzyme-injection-type glucose biosensor; materials used in the device have the advantages of high temperature resistance and corrosion resistance; and the device has a simple structure, and is convenient to process and easy to produce in batches.
Description
Technical field
The utility model relates to electrochemical measuring technology, is a kind of three electrode assemblies that are used to detect concentration of glucose, specifically, is a kind of three electrode assemblies that are applicable to enzyme injecting type glucose biological sensor.
Background technology
Glucose is reactant and intermediate product common in the fermentation process, and the control to concentration of glucose in the sweat can be directly connected to product productive rate and quality.
At present, generally detect concentration of glucose with the glucose enzyme electrode biology sensor.The principle of glucose enzyme electrode biology sensor is an electrochemical reaction process; Electrochemical reaction takes place in glucose oxidase and glucose in reaction tank in this process; The electronics that generates forms electric current; Obtain current signal or voltage signal by testing circuit through electrode, thereby realize the detection of glucose.
With common glucose analysis method---the fixed sugared method of Fehling (Fehling), glucose oxidase-peroxidase-colourimetry are compared, and it is strong that the glucose enzyme electrode biology sensor has selectivity, is suitable for online; Speed is fast; But continued operation is easy and simple to handle, and visual result shows and the data record stores but measure; Environment and human factor influence plurality of advantages such as less to it, so the glucose enzyme electrode biology sensor obtains extensive studies and application.
At present, the glucose enzyme electrode biology sensor mainly contains two types: one type is the immobilised enzymes mode, is about to glucose oxidase and is fixed on the electrode; Another kind of is the enzyme injection system; Great majority research concentrates on the former, promptly on the enzyme immobilization technology.Traditional approach with respect to the glucose enzyme electrode biology sensor---enzyme immobilization mode, enzyme injecting type were proposed by Kriz in 1996, and with its commercialization.
The common ground of enzyme immobilization mode and enzyme injecting type glucose enzyme electrode biology sensor is: all adopt the enzyme electrode principle of electrochemical reaction.Difference is:
(1) enzyme immobilization mode:
The enzyme require immobilization technology is fixed on semi-permeable diaphragm or the electrode, and immobilization technology is complicated; The activity of enzyme is influenced by test solution, and enzyme membrane reduces with reaction activity gradually, needs constantly calibration; Need bubble to rise before enzyme membrane uses, about 45 minutes times spent, be difficult to online use; A kind of sensor of enzyme membrane can only detect a kind of material; Enzyme membrane costs an arm and a leg, and homemade about 100 yuan one, one in import hundreds of unit.
(2) enzyme injecting type:
Enzyme exists with the mode of enzyme liquid, is about to enzyme and adjuvant and is dissolved in buffering agent and processes, and is simple to operate; Enzyme exists with the enzyme liquid mode of " fresh ", and is independent with test solution, do not receive ectocine, do not need frequent calibration; Change different enzyme liquid, can detect different material, promptly a sensor can detect multiple material; Single detects, about 0.01 yuan of enzyme liquid cost.
And according to patent " enzyme injecting type the glucose biological sensor " (patent No.: 201010616224.7) can know that enzyme injecting type glucose biological sensor advantage is a lot, more and more receive people's favor.
Three electrode assemblies are important component parts of glucose biological sensor, are the critical components that detects concentration of glucose, directly have influence on the sensitivity of glucose biological sensor, degree of accuracy etc.And for commercialization enzyme electrode glucose biological sensor; Be two electrode assemblies of applying immobilized zymotechnic; Be three electrode assemblies of applying immobilized zymotechnic, so need a kind of three electrode assemblies that are applicable to enzyme injecting type glucose biological sensor.
Summary of the invention
The utility model provides a kind of three electrode assemblies that are applicable to enzyme injecting type glucose biological sensor, can well be applied to enzyme injecting type glucose biological sensor, for succeeding in developing of enzyme injecting type glucose biological sensor provides possibility.
The utility model adopts following technical scheme:
Three electrode assemblies that are used for enzyme injecting type glucose biological sensor, it comprises working electrode 1, to electrode 2, contrast electrode 3, damping fluid and enzyme liquid inlet 4, damping fluid and liquid to be measured inlet 5, liquid outlet 6, electrode fixed body 7, concrete annexation is:
Said working electrode 1, electrode 2 and contrast electrode 3 are fixed in the electrode fixed body 7, have constituted the electrochemical measurement three-electrode system jointly; Have damping fluid and enzyme liquid inlet 4, damping fluid and liquid to be measured inlet 5, liquid outlet 6 on the electrode fixed body 7; Said damping fluid and enzyme liquid inlet 4 is connected with the damping fluid bottle with enzyme liquid calibrated shot device; Said damping fluid and liquid to be measured inlet 5 are connected with the damping fluid bottle with liquid calibrated shot device to be measured; Said liquid outlet 6 is connected with waste liquid bottle;
Said working electrode 1, electrode 2 and contrast electrode 3 are mutually hexagonal angle between any two are fixed in the electrode fixed body 7.
Said working electrode 1 is shaped as cylindrical or annular, is processed by metal material or conductive material; Described metal material or conductive material comprise platinum, gold, silver, graphite.
Saidly be shaped as cylindrical or annular, process by metal material or conductive material to electrode 2; Described metal material or conductive material comprise platinum, gold, silver, graphite.
Said contrast electrode 3 is shaped as cylindrical or annular, is made up of Ag/AgCl.
Electrode fixed body 7 is shaped as cylindrical or square, is processed by high temperature resistant nonmetallic materials; Said high temperature resistant nonmetallic materials comprise polyetheretherketone PEEK, polysulfones.
It also can be used for the enzyme injecting type biology sensor of lactic acid or glutamic acid.
The utility model has the advantages that:
1, is applicable to enzyme injecting type glucose biological sensor;
2, material therefor is high temperature resistant, and is corrosion-resistant;
3, the utility model, simple in structure, easy to process, be easy to mass production;
Description of drawings
Fig. 1 is the section of structure of the utility model.
Among the figure: 1, working electrode, 2, to electrode, 3, contrast electrode, 4, damping fluid and enzyme liquid inlet, 5, damping fluid and liquid to be measured inlet, 6, liquid outlet, 7, the electrode fixed body.
Embodiment
As shown in Figure 1, the utility model structure is: working electrode 1, to electrode 2, contrast electrode 3, damping fluid and enzyme liquid inlet 4, damping fluid and liquid to be measured inlet 5, liquid outlet 6, electrode fixed body 7.
It is that 1mm is the hole of 2cm deeply that three diameters are arranged in the described electrode fixed body 7; Can be with AB glue with working electrode 1, electrode 2, contrast electrode 3 be bonded at respectively in three holes; Constituted the three-electrode system that is used for electrochemical measurement jointly; Wherein the effect of contrast electrode 3 is that a stable electrode potential is provided in measuring process, working electrode with electrode is formed current return, be used to measure the electric current that reaction generates.Also having three diameters in the electrode fixed body 7 in addition is that 2mm is the hole of 2cm deeply, is respectively damping fluid and enzyme liquid inlet 4, damping fluid and liquid to be measured inlet 5, liquid outlet 6.Said damping fluid and enzyme liquid inlet 4 is connected with the damping fluid bottle with enzyme liquid calibrated shot device; Said damping fluid and liquid to be measured inlet 5 are connected with the damping fluid bottle with liquid calibrated shot device to be measured; Said liquid outlet 6 is connected with waste liquid bottle.
Wherein, working electrode 1 is shaped as cylindrical or annular, can be processed by metal material or conductive material (such as platinum, gold, silver, graphite etc.).
Be shaped as cylindrical or annular to electrode 2, can process by metal material or conductive material (such as platinum, gold, silver, graphite etc.).
Electrode fixed body 7 is shaped as cylindrical or square, can be processed by high temperature resistant nonmetallic materials (such as polyetheretherketone (PEEK), polysulfones etc.).
Said working electrode 1, electrode 2 and contrast electrode 3 are mutually hexagonal angle between any two are fixed in the electrode fixed body 7.
The course of work of said apparatus is following:
Under the effect of pumping system; Glucose liquid calibrated shot to be measured device is sent into a certain amount of glucose liquid to be measured in the enzyme injecting type glucose biological sensor reaction tank through damping fluid and liquid to be measured inlet 5; Then; Enzyme liquid calibrated shot device through enzyme liquid and damping fluid inlet 4 with in the quantitative enzyme injecting type glucose biological sensor reaction tank, under working electrode 1, three-electrode system effect to electrode 2, contrast electrode 3 compositions, glucose and glucose oxidase generation enzymatic reaction; Produce and the proportional low current signal of concentration of glucose, this current signal is handled, is gathered by signal conditioning circuit and data acquisition circuit.
Claims (2)
1. be used for three electrode assemblies of enzyme injecting type glucose biological sensor, it is characterized in that: it comprises: working electrode (1), to electrode (2), contrast electrode (3), damping fluid and enzyme liquid inlet (4), damping fluid and liquid to be measured inlet (5), liquid outlet (6), electrode fixed body (7);
Said working electrode (1), electrode (2) and contrast electrode (3) are fixed in the electrode fixed body (7), have constituted the electrochemical measurement three-electrode system jointly; Have damping fluid and enzyme liquid inlet (4), damping fluid and liquid to be measured inlet (5), liquid outlet (6) on the electrode fixed body (7); Said damping fluid is connected with the damping fluid bottle with enzyme liquid calibrated shot device with enzyme liquid inlet (4); Said damping fluid and liquid to be measured enter the mouth (5) be connected with the damping fluid bottle with liquid calibrated shot device to be measured; Said liquid outlet (6) is connected with waste liquid bottle.
2. three electrode assemblies that are used for enzyme injecting type glucose biological sensor according to claim 1 is characterized in that: said working electrode (1), electrode (2) and contrast electrode (3) are mutually hexagonal angle between any two are fixed in the electrode fixed body (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220071992 CN202631476U (en) | 2012-02-29 | 2012-02-29 | Three-electrode device used for enzyme-injection-type glucose biosensor |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201220071992 CN202631476U (en) | 2012-02-29 | 2012-02-29 | Three-electrode device used for enzyme-injection-type glucose biosensor |
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| CN202631476U true CN202631476U (en) | 2012-12-26 |
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| CN 201220071992 Expired - Fee Related CN202631476U (en) | 2012-02-29 | 2012-02-29 | Three-electrode device used for enzyme-injection-type glucose biosensor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117969629A (en) * | 2024-04-02 | 2024-05-03 | 四川省疾病预防控制中心(四川省预防医学科学研究院四川省卫生监测检验中心) | System and method for detecting physiological index |
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2012
- 2012-02-29 CN CN 201220071992 patent/CN202631476U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117969629A (en) * | 2024-04-02 | 2024-05-03 | 四川省疾病预防控制中心(四川省预防医学科学研究院四川省卫生监测检验中心) | System and method for detecting physiological index |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121226 Termination date: 20140229 |