CN202595145U - Collector for viable sperms of testicular tissues - Google Patents

Collector for viable sperms of testicular tissues Download PDF

Info

Publication number
CN202595145U
CN202595145U CN 201220219131 CN201220219131U CN202595145U CN 202595145 U CN202595145 U CN 202595145U CN 201220219131 CN201220219131 CN 201220219131 CN 201220219131 U CN201220219131 U CN 201220219131U CN 202595145 U CN202595145 U CN 202595145U
Authority
CN
China
Prior art keywords
card base
tubular edge
collector
plane
scoop
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CN 201220219131
Other languages
Chinese (zh)
Inventor
陈子江
李�城
吴克良
马金龙
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shandong University
Original Assignee
Shandong University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shandong University filed Critical Shandong University
Priority to CN 201220219131 priority Critical patent/CN202595145U/en
Application granted granted Critical
Publication of CN202595145U publication Critical patent/CN202595145U/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Landscapes

  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model relates to a collector for viable sperms of testicular tissues. The collector comprises a cylindrical edge of which the inside is fixedly provided with a grinding screen, and outer clamping legs and inner clamping legs which are arranged on the lower part of the cylindrical edge, wherein clamping grooves are formed between the outer clamping legs and the inner clamping legs. The collector for the viable sperms of the testicular tissues has the advantages that the collector is reasonably designed, and can be matched with most of bearing vessels for experiments and inspection; the collector is easy to operate and master, so that time required by operation is shortened; the collector is short in grinding time, so that mechanical stress has small influences on samples; and the collector is low in cost, and one collector can be considered to be used at a time, namely the collector can be discarded after being used for one time, so that cross pollution of the samples can be prevented.

Description

A kind of testis tissue sperm collector alive
Technical field
The utility model relates to biological technical field, is specifically related to a kind of testis tissue sperm collector alive.
Background technology
At present, in education experiment and medical inspection, for the live collection of sperm of micro-testis tissue, because of the micro-characteristic of its sample (for example, general sampling amount for less than 0.5cm length) has determined its collection method to be not easy to use grinding machinery to carry out mechanical mill.Current methods mainly is that the testis tissue convoluted seminiferous tubule is peeled off and pulverized to manual pin type, carries out centrifugal collection then.Peel manually from pulverising step in, exist a lot of deficiencies: 1, peel manually from and crushing process in, poor stability is very easily overturned sample.2, manipulation in vitro overlong time, cooling obviously influence the activity of testicular sperm in the sample.3, the easy crossed contamination of sample.4, operation steps and front and back phase clean loaded down with trivial details.
Therefore, existence is to the demand of testis tissue sperm collector alive.
The utility model content
To the deficiency of prior art, the utility model provides a kind of testis tissue convenient, that cost is low safe in utilization sperm collector alive.
The testis tissue that the utility model provides sperm collector alive comprises that internal fixation has tubular edge that grinds net and outer card base and the interior card base that is arranged on said tubular edge lower part, forms draw-in groove between said outer card base and the said interior card base.
The vertical outside of perhaps pointing to said tubular edge of said outer card base with the plane at said tubular edge; The vertical inboard of perhaps pointing to said tubular edge of card base in said with the plane at said tubular edge.
Preferably, said outer card base is vertical with the plane at said tubular edge, and the angle between the plane at said interior card base and said tubular edge is 45 degree ~ 90 degree.
More preferably, said outer card base is vertical with the plane at said tubular edge, and said interior card base is also vertical with the plane at said tubular edge.
The length of said outer card base and said interior card base is respectively 5 ~ 10mm.
The aperture of the mesh of said grinding net is 5 ~ 300 μ m.
Said grinding net be shaped as concave surface or planeform.
Said tubular edge, said outer card base, said interior card base and said grinding net are processed by the material that is selected from plastics and metal respectively.
Said tubular edge and said outer card base, said in card base one-body molded.
Said scoop further comprises the grinding rod that is used with said grinding net; Said grinding rod is processed by the material that is selected from glass and metal.
Said scoop further comprises the carrying container with raised edges, and the raised edges of said carrying container is contained in the said draw-in groove.
The testis tissue of the utility model sperm collector alive is compared with prior art, has following outstanding beneficial effect: 1, reasonable in design, can mate most experiments and check with the carrying vessel.2, simple to operate, easy master has reduced the needed time of operation.3, milling time is short, and mechanical stress is less to the influence of sample.4, cost is lower, can consider disposable use, and instant throwing can not cause the sample crossed contamination.
Description of drawings
Fig. 1 is the vertical view of a kind of testis tissue sperm collector alive of the utility model.
Fig. 2 is the upward view of the testis tissue sperm collector alive of Fig. 1.
Fig. 3 is the center section plan of the testis tissue sperm collector alive of Fig. 1.
Fig. 4 is the vertical view of the another kind of testis tissue sperm collector alive of the utility model.
Fig. 5 is the center section plan of the testis tissue sperm collector alive of Fig. 4.
Fig. 6 for the testis tissue of the utility model grinding rod that the grinding net of sperm collector is used of living.
Fig. 7 is the center section plan that is connected with carrying container under a kind of testis tissue of the utility model sperm collector user mode alive.
Reference numeral:
1, tubular edge
2, grind net
3, outer card base
4, interior card base
5, draw-in groove
6, grinding rod
7, carrying container
Embodiment
Below in conjunction with accompanying drawing and embodiment the utility model is further described.Following embodiment only is used to explain the utility model, and unrestricted the utility model.
Can find out by Fig. 1 ~ 6; The testis tissue that the utility model provides sperm collector alive; Comprise outer card base 3 and interior card base 4 that internal fixation has the tubular edge 1 that grinds net 2 and is arranged on 1 bottom, said tubular edge, form draw-in groove 5 between said outer card base 3 and the said interior card base 4.
The height at said tubular edge 1 especially the restriction, as long as it not yielding and in use sample do not run off.It will be understood by those skilled in the art that meeting under the prerequisite of above-mentioned requirements that in order to practice thrift cost, the height at said tubular edge 1 is more little good more.
The thickness at said tubular edge 1 is restriction especially, if it not yielding and can the bottom be provided with said outer card base 3 and said in card base 4.It will be understood by those skilled in the art that meeting under the prerequisite of above-mentioned requirements that in order to practice thrift cost, the thickness at said tubular edge 1 is more little good more.
The diameter at said tubular edge 1 is restriction especially not.Be generally 2 ~ 10cm, be preferably 3 ~ 6cm.
Said fixing can adopt any way known in the art to realize.For example one-body molded or overlap joint is fixing etc.
The vertical outside of perhaps pointing to said tubular edge 1 of said outer card base 3 with the plane at said tubular edge 1; The vertical inboard of perhaps pointing to said tubular edge 1 of card base 4 in said with the plane at said tubular edge 1.
The outside at said tubular edge 1 with inboard refer to respectively beyond the edge of tubular described in the vertical view 1 the zone and with interior zone.
Preferably, said outer card base 3 is vertical with the plane at said tubular edge 1, said in angle between the plane at card base 4 and said tubular edge 1 be 45 degree ~ 90 degree (promptly pointing to the inboard perhaps vertical of said tubular edge 1) with the plane at said tubular edge 1.At this moment, said carrying container 7 is placed the carrying container of convenient and suitable more sizes.
More preferably, said outer card base 3 is vertical with the plane at said tubular edge 1, and said interior card base 4 is also vertical with the plane at said tubular edge 1, and promptly said outer card base 3 is a parallel relation with said interior card base 4.At this moment, said carrying container 7 is placed convenient and is contacted tightr with the projecting edge of carrying container 7.At this moment, the width of said draw-in groove 5 is generally 2 ~ 5mm.
It will be appreciated by those skilled in the art that; Card base 3 is vertical with the plane at said tubular edge 1 outside said; When card base 4 is also vertical with the plane at said tubular edge 1 in said (this moment, the cross section of said draw-in groove 5 was to be the orthogonal zone basically), the raised edges (circle) of the carrying container 7 of use must be complementary with said draw-in groove 5.At this moment, the raised edges of said carrying container 7 and said draw-in groove 5 are for the most closely contacting.
Those skilled in the art also are appreciated that; Card base 3 is vertical with the plane at said tubular edge 1 outside said; When the angle in said between the plane at card base 4 and said tubular edge 1 is spent less than 90 (this moment, the cross section of said draw-in groove 5 was to be the zone of right-angle triangle basically), the raised edges of said carrying container 7 can closely contact with said interior card base 4.
The plane at said tubular edge 1 refers to and vertical of the axle center at said tubular edge 1.
The cross-sectional shape of said outer card base 3 and said interior card base 4 has no particular limits.For example, the cross-sectional shape of said outer card base 3 and said interior card base 4 can be respectively rectangle or trilateral or wedge shape.
The length of said outer card base 3 and said interior card base 4 is respectively 5 ~ 10mm.
Among the application, said grinding net 2 refers to the parts that are fixed on 1 inner also part, said tubular edge or all have mesh.
The area in the zone with mesh of said grinding net 2 is restriction especially not, and it can occupy the whole of 1 inside, said tubular edge, also can occupy the part of 1 inside, said tubular edge.It will be understood by those skilled in the art that the area in the zone with mesh of said grinding net 2 is the bigger the better in order to accelerate mill efficiency.
The position in the zone with mesh of said grinding net 2 is restriction especially not, and it can be positioned at the central position at said tubular edge 1, also can be positioned at the non-central location at said tubular edge 1.It will be understood by those skilled in the art that for easy to use the optimum seeking site in the zone with mesh of said grinding net 2 is positioned at the central position at said tubular edge 1.
The aperture of the mesh of said grinding net 2 is 5 ~ 300 μ m, is preferably 70 ~ 150 μ m.
Said grinding net 2 be shaped as concave surface or planeform.Preferably, for ease, said grinding net 2 be shaped as concave.
Said tubular edge 1, said outer card base 3, said in card base 4 and said grinding net 2 process by the material that is selected from plastics and metal respectively.
Said plastic material includes but not limited to: Vilaterm (PE), Vestolen PP 7052 (PP), SE (PVC), PS (PS), acrylonitrile-butadiene-styrene (ABS) (ABS), polymethylmethacrylate (PMMA), polyamide (PA).
Said metallic substance includes but not limited to: copper, iron, stainless steel, aluminum magnesium alloy, aluminium, tin.
Preferably, said tubular edge 1 and said outer card base 3, said interior card base 4 are one-body molded.At this moment, easily manufactured and have good intensity.
Said scoop further comprises the grinding rod 6 that is used with said grinding net 2; Said grinding rod 6 is processed by the material that is selected from glass and metal.
Said scoop further comprises the carrying container 7 with raised edges, and the raised edges of said carrying container 7 can be contained in the said draw-in groove 5.During use, fall in the said carrying container 7, be convenient to follow-up processing through the sample of said grinding net 2.
The testis tissue that the it will be understood by those skilled in the art that the utility model sperm collector of living also is applicable to the processing of other microcomponent's sample.
Embodiment 1
The tubular edge 1 of said scoop, outer card base 3, interior card base 4 and grinding net 2 are one-body molded, and material is Vestolen PP 7052 (PP) plastics.Said outer card base 3 is vertical with the plane at said tubular edge 1, and said interior card base 4 is also vertical with the plane at said tubular edge 1, and forming the cross section between said outer card base 3 and the said interior card base 4 is orthogonal draw-in groove 5 basically.The width of said draw-in groove 5 is 2mm.The length of said outer card base 3 and said interior card base 4 is respectively 5mm.Said grinding net 2 is a concave, and the mesh zone on it is positioned at the center at said tubular edge 1, and the aperture of mesh is 75 μ m.Select to use raised edges (vertically) can with the said draw-in groove 5 tight carrying containers 7 that contact.
During use, in above-mentioned carrying container 7, add experiment or the needed small quantity of reagent of checked operation or other solution (for example, 2mL contains the G-MOPS of 5%HSA) earlier, then above-mentioned scoop is snapped on the said carrying container 7.Because the raised edges and the said draw-in groove 5 of said carrying container 7 are complementary, therefore the raised edges of said carrying container 7 is included in the said draw-in groove 5 fully.To be placed on the zone with mesh of said grinding net 2 through the testis tissue sample (0.5cm amount) that subcutaneous testicular biopsy obtains; The grinding rod 6 that uses glass material to process grinds; Can with small quantity of reagent or other liquid sample be washed while grinding, be ground into organize fragment or the individual cells that need until sample and fall in the reagent or other solution in the said carrying container 7 of below.Then said carrying container 7 and said scoop are separated,, will organize fragment or free cell to cultivate and collect said scoop jettisoning.
Embodiment 2
The tubular edge 1 of said scoop, outer card base 3, interior card base 4 and grinding net 2 are one-body molded, and material is an aluminum magnesium alloy.Said outer card base 3 is vertical with the plane at said tubular edge 1, and the angle between the plane at said interior card base 4 and said tubular edge 1 is 45 degree, forms the draw-in groove 5 that the cross section is generally triangular between said outer card base 3 and the said interior card base 4.The length of said outer card base 3 and said interior card base 4 is respectively 5mm.Said grinding net 2 is a planeform, and the mesh zone on it is positioned at the center at said tubular edge 1, and the aperture of mesh is 75 μ m.Select to use the carrying container 7 that raised edges (vertically) can closely contact with the innermost of said draw-in groove 5 or select to use raised edges (vertically) can with the said interior card base 4 tight carrying containers 7 that contact.
During use, in above-mentioned carrying container 7, add experiment or the needed small quantity of reagent of checked operation or other solution (for example, 2mL contains the G-MOPS of 5%HSA) earlier, then above-mentioned scoop is snapped on the said carrying container 7.To be placed on the zone with mesh of said grinding net 2 through the testis tissue sample (0.5cm amount) that subcutaneous testicular biopsy obtains; The grinding rod 6 that uses the stainless steel metal material to process grinds; Can with small quantity of reagent or other liquid sample be washed while grinding, be ground into organize fragment or the individual cells that need until sample and fall in the reagent or other solution in the said carrying container 7 of below.Then said carrying container 7 and said scoop are separated, said scoop is cleaned the back use, will organize fragment or free cell to cultivate and collect.
Embodiment 3
The tubular edge 1 of said scoop, outer card base 3 and interior card base 4 are one-body molded, and material is Vilaterm (PE), and the material of said grinding net 2 is a magnalium, and fixing with said tubular edge 1 overlap joint.Angle between the plane at said outer card base 3 and said tubular edge 1 is 100 degree (pointing to the outside at said tubular edge 1); Angle in said between the plane at card base 4 and said tubular edge 1 is 80 degree (pointing to the inboard at said tubular edge 1), forms the draw-in groove 5 that the cross section is generally triangular between said outer card base 3 and the said interior card base 4.The length of said outer card base 3 and said interior card base 4 is respectively 5mm.Said grinding net 2 is a concave, and the mesh zone on it is positioned at the center at said tubular edge 1, and the aperture of mesh is 75 μ m.Select to use the carrying container 7 that raised edges (vertically) can closely contact with the innermost of said draw-in groove 5 or select to use raised edges (vertically) can with the said interior card base 4 tight carrying containers 7 that contact.
During use, in above-mentioned carrying container 7, add experiment or the needed small quantity of reagent of checked operation or other solution (for example, 2mL contains the G-MOPS of 5%HSA) earlier, then above-mentioned scoop is snapped on the said carrying container 7.To be placed on the zone with mesh of said grinding net 2 through the testis tissue sample (0.5cm amount) that subcutaneous testicular biopsy obtains; The grinding rod 6 that uses glass material to process grinds; Can with small quantity of reagent or other liquid sample be washed while grinding, be ground into organize fragment or the individual cells that need until sample and fall in the reagent or other solution in the said carrying container 7 of below.Then said carrying container 7 and said scoop are separated,, will organize fragment or free cell to cultivate and collect said scoop jettisoning.
It will be understood by those skilled in the art that under the instruction of this specification sheets, can make some modifications or variation the utility model.These modifications and variation also should be within the utility model claim institute restricted portions.

Claims (10)

1. a testis tissue sperm collector alive is characterized in that, said scoop comprises that internal fixation has tubular edge that grinds net and outer card base and the interior card base that is arranged on said tubular edge lower part, forms draw-in groove between said outer card base and the said interior card base.
2. scoop according to claim 1 is characterized in that, the vertical outside of perhaps pointing to said tubular edge with the plane at said tubular edge of said outer card base; The vertical inboard of perhaps pointing to said tubular edge of card base in said with the plane at said tubular edge.
3. scoop according to claim 2 is characterized in that, said outer card base is vertical with the plane at said tubular edge, and the angle between the plane at said interior card base and said tubular edge is 45 degree ~ 90 degree.
4. scoop according to claim 3 is characterized in that, said outer card base is vertical with the plane at said tubular edge, and said interior card base is also vertical with the plane at said tubular edge.
5. according to claim 1 or 4 described scoops, it is characterized in that the length of said outer card base and said interior card base is respectively 5 ~ 10mm; The aperture of the mesh of said grinding net is 5 ~ 300 μ m.
6. scoop according to claim 5 is characterized in that, said grinding net be shaped as concave surface or planeform.
7. scoop according to claim 6 is characterized in that, said tubular edge, said outer card base, said interior card base and said grinding net are processed by the material that is selected from plastics and metal respectively.
8. scoop according to claim 7 is characterized in that, said tubular edge and said outer card base, said in card base one-body molded.
9. scoop according to claim 8 is characterized in that, said scoop further comprises the grinding rod that is used with said grinding net; Said grinding rod is processed by the material that is selected from glass and metal.
10. scoop according to claim 9 is characterized in that said scoop further comprises the carrying container with raised edges, and the raised edges of said carrying container is contained in the said draw-in groove.
CN 201220219131 2012-05-15 2012-05-15 Collector for viable sperms of testicular tissues Expired - Lifetime CN202595145U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201220219131 CN202595145U (en) 2012-05-15 2012-05-15 Collector for viable sperms of testicular tissues

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201220219131 CN202595145U (en) 2012-05-15 2012-05-15 Collector for viable sperms of testicular tissues

Publications (1)

Publication Number Publication Date
CN202595145U true CN202595145U (en) 2012-12-12

Family

ID=47312700

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 201220219131 Expired - Lifetime CN202595145U (en) 2012-05-15 2012-05-15 Collector for viable sperms of testicular tissues

Country Status (1)

Country Link
CN (1) CN202595145U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102660455A (en) * 2012-05-15 2012-09-12 陈子江 Testis live sperm collector

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102660455A (en) * 2012-05-15 2012-09-12 陈子江 Testis live sperm collector

Similar Documents

Publication Publication Date Title
CN202595145U (en) Collector for viable sperms of testicular tissues
CN206289239U (en) DNA of plants rapid extraction corollary apparatus
CN203997442U (en) A kind of liquid nitrogen container cryopreservation tube holding rack
CN102660455A (en) Testis live sperm collector
CN202124622U (en) Automatic cell separating device for medical use
CN105170280B (en) A kind of liquid nitrogen grinding system
CN103376310A (en) Portable parazoon examination box
CN214844071U (en) Sampling box
CN205562942U (en) Glass carrier sheet
CN202896622U (en) Sampling cart for objects to be inspected
CN204799312U (en) Sample collector is taken out in inspection and quarantine
CN103305401A (en) Stem cell extraction device
CN204356337U (en) Placenta material extraction element
CN203154320U (en) Multifunctional device for extracting, separating and refining traditional Chinese medicine
CN202519250U (en) Multipurpose microorganism testing slide
CN202485960U (en) Disposable tissue grinding tube
CN203870323U (en) Multipurpose glass slide
CN201157237Y (en) Separator for removing core of apple
CN202822126U (en) Heater turning arm quick-open mechanism
CN2693227Y (en) Simple separate placenta cornmill
CN202530087U (en) Sampling device for cell culture
CN202415528U (en) Treatment vessel for manually chopping physical tissues
CN203388149U (en) Freezing tube lifting basket for liquid nitrogen container
CN214991538U (en) Novel biological extractor
CN209264316U (en) A kind of composite and multi-purpose sampler

Legal Events

Date Code Title Description
C14 Grant of patent or utility model
GR01 Patent grant
EE01 Entry into force of recordation of patent licensing contract

Assignee: Shandong Shanda Affiliated Reproduction Hospital Co.,Ltd.

Assignor: Chen Zijiang|Shandong University

Contract record no.: 2013370000232

Denomination of utility model: Testis live sperm collector

Granted publication date: 20121212

License type: Exclusive License

Record date: 20131108

LICC Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model
CX01 Expiry of patent term
CX01 Expiry of patent term

Granted publication date: 20121212