CN202492482U - Ethanol dropping device for low temperature ethanol protein separation device - Google Patents
Ethanol dropping device for low temperature ethanol protein separation device Download PDFInfo
- Publication number
- CN202492482U CN202492482U CN2012200418625U CN201220041862U CN202492482U CN 202492482 U CN202492482 U CN 202492482U CN 2012200418625 U CN2012200418625 U CN 2012200418625U CN 201220041862 U CN201220041862 U CN 201220041862U CN 202492482 U CN202492482 U CN 202492482U
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- CN
- China
- Prior art keywords
- ethanol
- shower nozzle
- spray nozzle
- dropping feeder
- spray material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 119
- 108090000623 proteins and genes Proteins 0.000 title abstract description 18
- 102000004169 proteins and genes Human genes 0.000 title abstract description 18
- 238000000926 separation method Methods 0.000 title abstract description 6
- 239000007921 spray Substances 0.000 claims abstract description 29
- 239000000463 material Substances 0.000 claims abstract description 28
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 239000012528 membrane Substances 0.000 claims description 3
- 230000036425 denaturation Effects 0.000 abstract description 3
- 238000004925 denaturation Methods 0.000 abstract description 3
- 238000005507 spraying Methods 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 2
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 description 10
- 210000002381 plasma Anatomy 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 5
- 229910001220 stainless steel Inorganic materials 0.000 description 4
- 239000010935 stainless steel Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 108010017384 Blood Proteins Proteins 0.000 description 3
- 102000004506 Blood Proteins Human genes 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 230000001476 alcoholic effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000012460 protein solution Substances 0.000 description 3
- 230000008021 deposition Effects 0.000 description 2
- 238000004880 explosion Methods 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 101000886418 Drosophila melanogaster GATA-binding factor C Proteins 0.000 description 1
- 101000651439 Homo sapiens Prothrombin Proteins 0.000 description 1
- 108090000190 Thrombin Proteins 0.000 description 1
- 238000005411 Van der Waals force Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003398 denaturant Substances 0.000 description 1
- 208000002925 dental caries Diseases 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 229940039715 human prothrombin Drugs 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000000247 postprecipitation Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 229960004072 thrombin Drugs 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
Images
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- Peptides Or Proteins (AREA)
Abstract
The utility model relates to an ethanol dropping device for a low temperature ethanol protein seperation device. The ethanol dropping device comprises a spray nozzle, a piston pump and a connecting pipe which is connected between the spray nozzle and the piston pump; the spray nozzle is a cylindrical vertical tube; the upper end of the vertical tube is an opening, and the lower end of the vertical tube is sealed; the opening end is connected with the connecting pipe; and material spraying ports are arranged on the pipe wall at one side of the spray nozzle in an arraying manner. The spray nozzle is arranged under a material liquid level of the separation device, and the material spraying ports on the spray nozzle face towards the inner wall of the separation device. The ethanol dropping device provided by the utility model can enable ethanol to be simultaneously sprayed toward a tank wall in a three-dimensional manner under the material liquid level on multiple horizontal surfaces; and the ethanol is slowly and uniformly added to a protein aqueous solution along with liquid flow with higher linear velocity, thereby avoiding the protein denaturation caused by the overhigh concentration of local ethanol and achieving the requirement of a productive technology.
Description
Technical field
The utility model relates to a kind of ethanol Dropping feeder, particularly relates to a kind of ethanol Dropping feeder that is used for cold ethanol albumen sepn method.
Background technology
It is to utilize the physico-chemical property of different protein ingredients to distinguish that plasma proteins separates, like molecular size, density height, surface tissue, state-of-charge etc., that it is separated from each other.Physical method comprises ultra centrifugal, ultrafiltration, absorption, gel-filtration, electrophoresis isoelectrofocusing and selectivity denaturing treatment etc.; Chemical process mainly is the interaction that utilizes between protein molecular and protein molecular, protein molecular and water molecules and the various ion, handles or changes its solvability, makes some protein molecular deposition, other dissolvings, thus they are separated.
The Cohn cold ethanol method is the albumen sepn method that domestic and international most producer adopts.Its principle is in protein solution, to add water-miscible organic solvent ethanol; To reduce the activity of water molecules, reduce the specific inductivity of solution, around protein molecular, repel water molecules; Make between the protein molecular through the interaction of polar group, under the Van der Waals force effect, condense.
Since ethanol have specific inductivity low, with water be prone to miscible, can in low temperature, use, under home and working conditions, not have explosion hazard, lower molecular weight, relative inertness chemically, low toxicity, inexpensive, be easy to get and advantage such as bacteriostatic action, the cold ethanol partition method is still adopted in extensive so far plasma proteins separation basically.
When albumen sepn is operated, generally be protein concentration, pH value and the ionic strength that earlier blood plasma (or supernatant of certain component) is transferred to regulation, add ethanol then.Along with the increase of alcohol concn, the specific inductivity of protein soln reduces gradually, and albumen solubility sharply descends, and albumen is pressed bulk of molecule order elder generation postprecipitation.
In plasma proteins cold ethanol partition method, alcoholic acid adding mode is one of key.Because ethanol is protein precipitant still not; Also be protein denaturant simultaneously, so must slowly evenly add while stirring when adding ethanol; Do not make the ethanol partial concn too high as far as possible; To prevent that the too high ethanol of partial concn from causing that natural inhibitor is inactivated in protein denaturation and the blood plasma, keep proteinic natural bioactive and validity, avoid the thrombin activation that in the operation of separation of human Prothrombin Complex Concent-(PCC), possibly run into; More be prone to simultaneously form bigger protein grain, be easy to centrifugal or the press filtration separation.
In the albumen sepn process, adopt shower nozzle on liquid level, directly to spray the alcoholic acid method because the fluid surface alcohol concn is high, and the result makes protein denaturation, separating effect is undesirable.And adopt the cruciform frame that has aperture under liquid level, to spray ethanol, and also carry out owing to being sprayed at a horizontal plane, still can not solve the too high technical barrier of ethanol partial concn fully, cause the protein product yield low.
Summary of the invention
The purpose of the utility model is the deficiency to prior art, and a kind of ethanol Dropping feeder that is used on the cold ethanol albumen sepn equipment is provided, and this device adopts three-dimensional mode of spraying, and ethanol is slowly dropped in the protein solution equably.
The technical scheme that the utility model adopts is:
A kind of ethanol Dropping feeder that is used on the cold ethanol albumen sepn equipment; Comprise a shower nozzle, a piston pump, and be connected the pipe connecting between shower nozzle and the piston pump, it is characterized in that described shower nozzle is a columniform vertical tube; The open upper end of vertical tube; Lower end closed, opening end links to each other with pipe connecting, on the tube wall of shower nozzle one side, is provided with the spray material port of arrayed.
Wherein, described spray material port is set to funnel-shaped hole, spray material port aperture 1mm.
Further, the tube wall of the utility model shower nozzle one side is provided with 2～4 row, 8～18 row spray material ports, and the angle of adjacent two row spray material port medullary rays is 45 °, line-spacing 50～100mm between spray material port.
The utility model places above-mentioned shower nozzle under the material liquid surface of separating device, and the spray material port on the shower nozzle is towards the inwall of separating device.
The utility model also is equipped with flow director and strainer on the pipe connecting of said ethanol Dropping feeder, the filter membrane aperture in the strainer is 0.22 μ m or 0.45 μ m.
The shower nozzle of the utility model adopts stainless steel, and the jet length under material liquid surface is 0.2～0.5m, can satisfy the needs of different diameter tank body, realizes the uniformity coefficient that ethanol drips to greatest extent.The flow control meter can be implemented in line monitoring alcoholic acid temperature, density, real-time traffic and integrated flow, can set parameters such as real-time flow rate, final ethanol dosage simultaneously, is used for the stroke and the operation of piston pump.Piston pump adopts the stainless steel piston cavity, and clean compressed air is a power, satisfies the requirement of explosion proof that ethanol drips.
Adopt the ethanol Dropping feeder of the utility model; Ethanol sprays to tank skin simultaneously on a plurality of horizontal planes under material liquid surface; Can join in the protein solution slowly, equably along with the bigger flow of LV; Avoid the protein-denatured generation that causes because of local alcohol concn is too high, reached manufacturing technique requirent.Because the dropping process is in closed pipeline, to carry out, the control of albumen sepn parameter is accurate, and facility investment reduces; Form the mother liquor carried secretly in the deposition still less, precipitated solid content is 25～30%, and production process is easy to control; Can reduce production process and pollute, improve the quality of products and product yield, simultaneously; Can also improve production environment, shorten the running time, reduce production costs.
After in cold ethanol albumen sepn method, using the ethanol Dropping feeder of the utility model; The BSA productive rate is brought up to 28.5～30.5g/L blood plasma from 25.7～27.5g/L blood plasma, and the Tegeline productive rate is brought up to 0.45～0.55g/L blood plasma from 0.37～0.42g/L blood plasma.
Description of drawings
Fig. 1 is used in the structural representation of the ethanol Dropping feeder on the cold ethanol albumen sepn equipment for the utility model.
Fig. 2 is the structural representation of shower nozzle 2 in the ethanol Dropping feeder.
Fig. 3 is the A-A view of Fig. 2.
Among the figure: 1. spray material port; 2. shower nozzle; 3. pipe connecting; 4. strainer; 5. flow control meter; 6. piston pump; 7. cold ethanol basin; 8. whisking appliance; 9. retort.
Embodiment
A kind of ethanol Dropping feeder that is used on the cold ethanol albumen sepn equipment, as shown in Figure 1, constitute by shower nozzle 2, pipe connecting 3, strainer 4, flow control meter 5, piston pump 6 and cold ethanol basin 7.
The structure of shower nozzle 2 such as Fig. 2, shown in Figure 3 make with the 316L stainless steel, and be cylindrical, diameter 50mm, and long 1000mm, the lower end sealing, the upper end links to each other with pipe connecting 3, arranges spray material ports 1 in the lateral longitudinal of shower nozzle 2 to being arranged with 3, laterally totally 8～18 goes.Spray material port 1 is a funnel-shaped hole, the diameter 1mm in hole, and the angle of 3 row's spray material ports, 1 medullary ray is 45 °.
As shown in Figure 1, shower nozzle 2 is installed in below the material liquid surface of retort 9, spray material port 1 is towards tank skin.Piston pump 6 is extracted cold ethanol out from cold ethanol basin 7; Through flow control meter 5 dominant discharge is 0.5～3.0L/min; Strainer 4 elimination fine impurity are got in shower nozzles 2 cavitys by pipe connecting 3, from spray material port 1 dorsad the direction of whisking appliance 8 spray to the tank skin of retort 9.
Claims (8)
1. ethanol Dropping feeder that is used on the cold ethanol albumen sepn equipment; Comprise a shower nozzle, a piston pump, and be connected the pipe connecting between shower nozzle and the piston pump, it is characterized in that described shower nozzle is a columniform vertical tube; The open upper end of vertical tube; Lower end closed, opening end links to each other with pipe connecting, on the tube wall of shower nozzle one side, is provided with the spray material port of arrayed.
2. ethanol Dropping feeder according to claim 1 is characterized in that described spray material port is a funnel-shaped hole, spray material port aperture 1mm.
3. ethanol Dropping feeder according to claim 1 and 2 is characterized in that the tube wall of shower nozzle one side is provided with 2～4 row, 8～18 row spray material ports.
4. ethanol Dropping feeder according to claim 3, the angle that it is characterized in that adjacent two row spray material port medullary rays is 45 °.
5. ethanol Dropping feeder according to claim 1 is characterized in that described shower nozzle places under the material liquid surface of separating device.
6. ethanol Dropping feeder according to claim 1 is characterized in that spray material port on the said shower nozzle is towards the inwall of separating device.
7. ethanol Dropping feeder according to claim 1 is characterized in that on described pipe connecting, flow director being installed.
8. ethanol Dropping feeder according to claim 1 is characterized in that also comprising a strainer, and said strainer is installed on the pipe connecting, and the filter membrane aperture in the strainer is 0.22 μ m or 0.45 μ m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012200418625U CN202492482U (en) | 2012-02-10 | 2012-02-10 | Ethanol dropping device for low temperature ethanol protein separation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012200418625U CN202492482U (en) | 2012-02-10 | 2012-02-10 | Ethanol dropping device for low temperature ethanol protein separation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202492482U true CN202492482U (en) | 2012-10-17 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012200418625U Expired - Fee Related CN202492482U (en) | 2012-02-10 | 2012-02-10 | Ethanol dropping device for low temperature ethanol protein separation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202492482U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112521486A (en) * | 2020-12-22 | 2021-03-19 | 博雅生物制药(广东)有限公司 | Production method for separating human serum albumin from ethanol at low temperature under real-time control of ethanol |
| CN115386002A (en) * | 2022-09-06 | 2022-11-25 | 浙江海康生物制品有限责任公司 | Immunoglobulin production device and production process |
-
2012
- 2012-02-10 CN CN2012200418625U patent/CN202492482U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112521486A (en) * | 2020-12-22 | 2021-03-19 | 博雅生物制药(广东)有限公司 | Production method for separating human serum albumin from ethanol at low temperature under real-time control of ethanol |
| CN115386002A (en) * | 2022-09-06 | 2022-11-25 | 浙江海康生物制品有限责任公司 | Immunoglobulin production device and production process |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121017 Termination date: 20210210 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |