CN202433376U

CN202433376U - Micropore plate capable of performing multi-index detection

Info

Publication number: CN202433376U
Application number: CN201120541592XU
Authority: CN
Inventors: 刘兴旺; 程晓雷; 叶兴旺
Original assignee: HOB BIOTECH GROUP CO Ltd
Current assignee: HOB BIOTECH GROUP CO Ltd
Priority date: 2011-12-22
Filing date: 2011-12-22
Publication date: 2012-09-12
Anticipated expiration: 2021-12-22

Abstract

The utility model discloses a micropore plate capable of performing multi-index detection. Various antigens or antibodies are coated inside micropores of the micropore plate, and only one kind of antigens or antibodies is coated inside each micropore. According to the micropore plate, a plurality of indexes are coated in one micropore plate and can be detected simultaneously, the operation method is the same with a single index detection method and is simple and practicable, and defects of the single index detection are overcome without increase of equipment or operating steps.

Description

A kind of microwell plate that carries out many indexs detections

Technical field

The utility model relates to enzyme linked immunological absorption detection range, particularly, relates to enzyme linked immunological absorption detection and uses microwell plate, relates in particular to a kind of microwell plate that many indexs detect that carries out.

Background technology

(Enzyme-linked immunosorbent assay is that a kind of susceptibility is high ELISA), high specificity, the laboratory diagnosis method of good reproducibility in enzyme linked immunological absorption detection.Because its stable reagent, the easy preservation, easy and simple to handle, the result judges factors such as more objective, has been widely used in each field of immunological test.

Existing ELISA detection method majority all is the detection of single index; The detection method of single index will be carried out many indexs and detected (as: tumor markers, TORCH, infectious disease, food allergen, autoantibody etc.); A sample just needs the plurality of reagents box to detect simultaneously, operates complicatedly, and cost is expensive; Time-consuming effort again is difficult to satisfy clinical needs.

Along with the development of in-vitro diagnosis industry, to the raising of the comprehensive requirement of diagnostic result, the above-mentioned detection method of utilizing single index is carried out many indexs and is detected and can not satisfy corresponding demand.

The utility model content

The purpose of the utility model is to overcome the shortcoming and defect of above-mentioned prior art, and a kind of microwell plate that many indexs detect that can satisfy again simple to operate, with low cost is provided.

To achieve these goals, the utility model adopts following technical scheme:

A kind of microwell plate that carries out many indexs detections, the micropore endoperidium of said microwell plate has multiple different antigens or antibody, and only encapsulates a kind of antigen or antibody in each micropore.

Further, remove the described micropore that is coated with multiple different antigens or antibody, also have part micropore endoperidium to be useful on the standard antigen or the antibody of the variable concentrations of production standard curve on the said microwell plate.

Further, said microwell plate is 96 hole microwell plates, and the number cells of said standard antigen that is coated with the variable concentrations that is used for the production standard curve or antibody is 4-12, and the said number cells that is coated with multiple different antigens or antibody is 2-90.

Compared with prior art, the utlity model has following beneficial effect:

1, the microwell plate of the utility model can carry out the detection of multiple index simultaneously, and is the same with single index detection method, need not special devices; Also need not to increase operation steps, general laboratory all can be carried out, and is simple to operate, with low cost; The disposable acquisition of several data has improved detection efficiency, has reduced the detection error, technical also simple possible; Good reproducibility, reliability is high, and the product processes that produces is simple, realizes extensive, stable production easily.

2, adopt typical curve can realize quantitative measurment.

3, the microwell plate of the utility model can carry out the multiple index detection about 90 kinds at most simultaneously, and time saving and energy saving, detection efficiency improves greatly.

Through accompanying drawing and embodiment, the technical scheme of the utility model is done further detailed description below.

Description of drawings

Fig. 1 is the structural representation that the utility model can carry out the microwell plate of many indexs detections.

Embodiment

Describe below in conjunction with the preferred embodiment of accompanying drawing, should be appreciated that preferred embodiment described herein only is used for explanation and explains the utility model, and be not used in qualification the utility model the utility model.

The utility model has multiple different antigens or antibody at the micropore endoperidium of microwell plate, and only encapsulates a kind of antigen or antibody in each micropore, can realize detecting simultaneously multiple index.

Remove the above-mentioned micropore that is coated with multiple different antigens or antibody, also have part micropore endoperidium to be useful on the standard antigen or the antibody of the variable concentrations of production standard curve on the microwell plate,, can realize detection by quantitative through typical curve.

With 96 hole microwell plates is example; Being coated with the standard antigen of the variable concentrations that is used for the production standard curve or the number cells of antibody is 4-12; Can produce suitable typical curve, other micropores can be used for being coated with multiple different antigens or antibody, can realize detecting simultaneously 90 kinds of indexs at most.

The preparation method of above-mentioned microwell plate is following:

1, different types of antigen or antibody are dissolved in carbonic acid (or phosphoric acid, the acetic acid) damping fluid mixing, preparation coating buffer by certain concentration.

2, the coating buffer for preparing is joined (every hole 50-200 μ l) in the micropore plate hole by certain arrangement sequence, every hole adds a kind of antigen or antibody, and 4 ℃ of incubated overnight (or 37 ℃ hatched 1-5 hour) encapsulate.

3, be dissolved in phosphate buffer and wash plate with tween after, in microwell plate, add confining liquid (every hole 50-200 μ l) again, 4 ℃ of incubated overnight (or room temperature, 37 ℃ hatched 1-5 hour) are sealed.

4, discard liquid in the hole, room temperature or 37 ℃ of dry microwell plates are enclosed microwell plate in the aluminium foil bag after the drying.

Be that example specifies with the microwell plate that detects 14 kinds of food allergen IgG antibody simultaneously below:

1,14 kinds of food allergen albumen (beef, chicken, cod, corn, crab, egg white/yolk, mushroom, milk, pork, rice, shrimp, soybean, tomato, wheat) and the standard antigen that is used for the production standard curve are dissolved in the carbonic acid buffer of 50mM by 10 μ g/ml; Mixing, the preparation coating buffer.

2, the coating buffer for preparing is joined in the 96 hole microwell plates (every hole 100 μ l) by Fig. 1 order (standard antigen 0, beef 1, chicken 2, cod 3, corn 4, crab 5, egg white/yolk 6, mushroom 7, milk 8, pork 9, rice 10, shrimp 11, soybean 12, tomato 13, the wheat 14 that are used for the production standard curve); 4 ℃ of incubated overnight encapsulate.

3, be dissolved in phosphate buffer and wash plate 3 times with tween after, in microwell plate, add confining liquid (phosphate buffer that contains 2%BSA) again, every hole 200 μ l, 4 ℃ of incubated overnight are sealed.

4, discard liquid in the hole, the dry microwell plate of ambient temperature overnight is enclosed microwell plate in the aluminium foil bag after the drying.

In sum; The utility model utilizes existing technology; Under the situation that does not increase equipment and operation steps; Developed a kind of microwell plate that can on a plate, carry out many indexs detections, can realize carrying out simultaneously many indexs fully and detect, for the diagnostic method of another practical is provided clinically.

What should explain at last is: the above is merely the preferred embodiment of the utility model; Be not limited to the utility model; Although the utility model has been carried out detailed explanation with reference to previous embodiment; For a person skilled in the art, it still can be made amendment to the technical scheme that previous embodiment is put down in writing, and perhaps part technical characterictic wherein is equal to replacement.All within the spirit and principle of the utility model, any modification of being done, be equal to replacement, improvement etc., all should be included within the protection domain of the utility model.

Claims

1. one kind can be carried out the microwell plate that many indexs detect, and it is characterized in that the micropore endoperidium of said microwell plate has multiple different antigens or antibody, and only encapsulates a kind of antigen or antibody in each micropore.

2. the microwell plate that carries out many indexs detections according to claim 1; It is characterized in that; Remove the described micropore that is coated with multiple different antigens or antibody, also have part micropore endoperidium to be useful on the standard antigen or the antibody of the variable concentrations of production standard curve on the said microwell plate.

3. the microwell plate that carries out many indexs detections according to claim 2; It is characterized in that; Said microwell plate is 96 hole microwell plates; The number cells of said standard antigen that is coated with the variable concentrations that is used for the production standard curve or antibody is 4-12, and the said number cells that is coated with multiple different antigens or antibody is 2-90.