CN202415545U - Continuous flow type microbial fermentation hydrogen generation device - Google Patents

Continuous flow type microbial fermentation hydrogen generation device Download PDF

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Publication number
CN202415545U
CN202415545U CN 201120523923 CN201120523923U CN202415545U CN 202415545 U CN202415545 U CN 202415545U CN 201120523923 CN201120523923 CN 201120523923 CN 201120523923 U CN201120523923 U CN 201120523923U CN 202415545 U CN202415545 U CN 202415545U
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fermentation
reactor
fermentation reactor
hydrogen generation
feed pump
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钱春香
王瑞兴
陈璐圆
陈春
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Southeast University
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Southeast University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/04—Bioreactors or fermenters specially adapted for specific uses for producing gas, e.g. biogas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/03—Means for pre-treatment of biological substances by control of the humidity or content of liquids; Drying
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products

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Abstract

The utility model relates to a continuous flow type microbial fermentation hydrogen generation device which consists of a raw material tank, a peristaltic feed pump, a fermentation reactor, a NaOH reactor, a gas dryer, a gas flowmeter and a gas storage device connected in series, wherein a stirrer is arranged in the fermentation reactor; and a waste liquid outlet of the fermentation reactor is connected with a waste liquid collection tank through the peristaltic feed pump. The reaction raw material continuously flows into the fermentation reactor from the raw material tank through the peristaltic feed pump to perform a biological fermentation hydrogen generation reaction; meanwhile, the fermentation waste liquid is continuously discharged out of the fermentation reactor through the peristaltic feed pump; and the feed speed is the same as the discharge speed to keep constant total quantity of liquid in the reactor. The device provided by the utility model has the characteristics of compact structure, continuous and efficient hydrogen generation, and energy saving.

Description

A kind of continuously streamed microbial fermentation device for producing hydrogen
Technical field
The utility model relates to the efficient ferment for hydrogen production device of a kind of mikrobe, and concrete is a kind of fermentation reaction apparatus of continuously streamed.
Background technology
The high speed development height of current world economy is according to anti-fossil energy, and its demand accounts for 80% of overall energy requirement, and limited fossil energy will be exhausted rapidly in the near future.Fossil energy is non-renewable, and the utilization of fossil energy can cause serious pollution to environment inevitably, and the renewable energy of development and utilization cleaning has just become the direction that various countries research institution endeavours to study since being derived from last century always.Hydrogen is regarded as the most potential clean energy in this century with its cleaning, efficient, safe, continuable characteristic.Hydrogen production process has a lot, but by contrast, biological hydrogen production has its special advantages.The bio-hydrogen production technology reaction conditions is gentle, low, the cleanliness without any pollution of energy consumption, and can be raw material with depleted biomass resource or organic waste water, can properly solve the contradiction of energy and environment, thereby becomes the hot research field that the various countries scientist focuses on.
Biological hydrogen production is a physiological action of utilizing mikrobe self, under certain envrionment conditions, obtains hydrogen through metabolism.Bio-hydrogen production technology mainly comprises optical drive process and two kinds of routes of fermentation and hydrogen production technology, and the former utilizes photosynthetic bacterium directly conversion of solar energy to be hydrogen, and its optical energy utilization efficiency is lower, the photoreactor difficult design, and cost is also higher; And the latter is the anaerobically fermenting that utilizes hydrogenogens, and its substrate conversion efficiency is high, and hydrogen-producing speed is fast, and reactor design is simple relatively, realizes using in short-term more easily with respect to the former.There are the characteristics of fermentation period than traditional written instructions fermentation, develop that a kind of to have efficient, as to continue hydrogen production potential microorganism continuous ferment for hydrogen production device be a kind of inexorable trend.
Summary of the invention
In order to solve the inefficient problem of batch fermentation that prior art exists, the utility model provides a kind of continuously streamed microbial fermentation device for producing hydrogen, this apparatus structure compact, product hydrogen continuous high-efficient.
The technical scheme of the utility model is: a kind of continuously streamed microbial fermentation device for producing hydrogen; Be composed in series by feed reservoir, wriggling fresh feed pump, fermentation reactor, NaOH reactor drum, gas flow dryer, gas meter, accumulator unit; Be provided with whisking appliance in the described fermentation reactor, the waste liquid outlet of fermentation reactor is connected with the waste collection pond through the wriggling discharging pump.The bottom of described fermentation reactor is provided with microfiltration membrane.
Beneficial effect:
(1) in continuous supplementation nutritive substance and matrix, successive discharging fermented liquid is effectively kept thalline activity and concentration, alleviates the accumulation of harmful meta-bolites, reaches the purpose that continues highly effective hydrogen yield.
(2) fermentation reactor bottom is provided with microfiltration membrane, is used to realize separating of thalline and fermented liquid, makes thalline effectively be trapped in inside reactor, has effectively improved cell concentration in the fermentation reactor, reaches the purpose that continues highly effective hydrogen yield.
(3) reaction raw materials continuously flows into from feed reservoir through the wriggling fresh feed pump and carries out the fermentative hydrogen production reaction the fermentation reactor; Fermented waste fluid is discharged fermentation reactor continuously through the wriggling discharging pump simultaneously; Input speed should be consistent with discharge velocity; Total amount of liquid is constant in the reactor drum, guarantees to continue in the reactor drum to produce hydrogen.The gas that fermentation reaction generates is discharged from reactor head, after through NaOH solution, realizes H 2And CO 2Separation, the speed and the total amount of producing hydrogen through gas meter on-line monitoring microbial fermentation.
Description of drawings
Fig. 1 is the structural representation of the utility model.
Among the figure: 1-feed reservoir, the 2-fresh feed pump of wriggling, 3-whisking appliance, 4-fermentation reactor, 5-microfiltration membrane, the 6-discharging pump of wriggling, 7-fermented liquid collecting tank, 8-NaOH reactor drum, 9-gas flow dryer, 10-gas meter, 11-accumulator unit.
Embodiment
A kind of continuously streamed microbial fermentation device for producing hydrogen; Be composed in series by feed reservoir 1, wriggling fresh feed pump 2, fermentation reactor 4, NaOH reactor drum 8, gas flow dryer 9, gas meter 10, accumulator unit 11; Be provided with whisking appliance 3 in the described fermentation reactor 4, the waste liquid outlet of fermentation reactor 4 is connected with waste collection pond 7 through wriggling discharging pump 6.
The bottom of described fermentation reactor 4 is provided with microfiltration membrane.
Mikrobe and nutritive substance, matrix are fed in the fermentation reactor 4, carry out fermentation and hydrogen production.When treating that its hydrogen-producing speed peaks; Fresh nutritive substance and matrix continuously flowed into from feed reservoir 1 through wriggling fresh feed pump 2 carry out the fermentative hydrogen production reaction the fermentation reactor 4; Fermented waste fluid is discharged fermentation reactor 4 continuously through wriggling discharging pump 6 simultaneously; Input speed should be consistent with discharge velocity, keeps fermentation reactor 4 interior total amount of liquid constant.Fermentation reactor 4 bottoms are provided with microfiltration membrane, are used to realize separating of thalline and fermented liquid, make thalline effectively be trapped in fermentation reactor 4 inside, and fermented waste fluid is expelled to waste collection pond 7.The gas that fermentation reaction generates is realized H through behind the NaOH solution 2And CO 2Separation.
Embodiment 1: the continuously streamed fermentation and hydrogen production
Use reactor volume to be the efficient ferment for hydrogen production device of the continuously streamed mikrobe of 10L in the laboratory, reactor bottom is provided with microfiltration membrane.With Clostridium baratii, clostridium butylicum, enteroaerogen ratio in 1: 1: 1, amount to 10% inoculum size, and the nutritive substance of 6L and matrix, throw in to fermentation reactor jointly.The component of nutritive substance and matrix and concentration are: peptone 10g/L, Carnis Bovis seu Bubali cream 2.4g/L, yeast extract 5.0g/L, Na 2HPO 44g/L, L-halfcystine 0.2g/L, L-cysteine hydrochloride one water 0.5g/L, resazurin (0.2%) 1mL/L, glucose 20g/L.Behind the speed stirring reaction 6h with 170rpm, begin to open wriggling fresh feed pump and wriggling discharging pump, the charging nutritive substance is as above consistent with substrate concn.Discharging and feeding rate are 5ml/min, and the hydrogen of miscarrying continuously continues 24h its hydrogen-producing speed of test and total amount.
Comparative Examples: batch fermentation produces hydrogen
Use reactor volume to carry out batch fermentation hydrogen manufacturing as the efficient ferment for hydrogen production device of the mikrobe of 10L in the laboratory, reactor bottom does not have microfiltration membrane.With Clostridium baratii, clostridium butylicum, enteroaerogen ratio in 1: 1: 1, amount to 10% inoculum size, and the nutritive substance of 6L and matrix, throw in to fermentation reactor jointly.The component of nutritive substance and matrix and concentration are: peptone 10g/L, Carnis Bovis seu Bubali cream 2.4g/L, yeast extract 5.0g/L, Na 2HPO 44g/L, L-halfcystine 0.2g/L, L-cysteine hydrochloride one water 0.5g/L, resazurin (0.2%) 1mL/L, glucose 20g/L.With the speed stirring reaction of 170rpm, the formula of criticizing is produced hydrogen, continues 24h its hydrogen-producing speed of test and total amount.
Adopt the device for producing hydrogen that originally continuously ferments to carry out the continuously streamed fermentation; Owing to behind 6h, start wriggling fresh feed pump and wriggling discharging pump, can continuous supplementation nutritive substance and matrix, and the continuous blow-down fermented waste fluid; Effectively keep thalline activity and concentration, alleviate the accumulation of harmful meta-bolites; In addition, fermentation reactor bottom is provided with microfiltration membrane, has realized separating of thalline and fermented liquid; Make thalline effectively be trapped in inside reactor; Effectively improved cell concentration in the fermentation reactor, behind 6h, still kept higher hydrogen-producing speed, attenuation trend has not taken place; Realized the purpose of lasting highly effective hydrogen yield, its hydrogen output is 4 times that batch fermentation produces hydrogen.

Claims (2)

1. continuously streamed microbial fermentation device for producing hydrogen; It is characterized in that: be composed in series by feed reservoir (1), wriggling fresh feed pump (2), fermentation reactor (4), NaOH reactor drum (8), gas flow dryer (9), gas meter (10), accumulator unit (11); Be provided with whisking appliance (3) in the described fermentation reactor (4), the waste liquid outlet of fermentation reactor (4) is connected with waste collection pond (7) through wriggling discharging pump (6).
2. according to the described continuously streamed microbial fermentation of claim 1 device for producing hydrogen, it is characterized in that: the bottom of described fermentation reactor (4) is provided with microfiltration membrane.
CN 201120523923 2011-12-15 2011-12-15 Continuous flow type microbial fermentation hydrogen generation device Expired - Fee Related CN202415545U (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103382436A (en) * 2013-06-24 2013-11-06 江苏元洲生物工程有限公司 Continuous perfusion device of fermentation tank
CN107488584A (en) * 2017-09-25 2017-12-19 南京律智诚专利技术开发有限公司 One kind is based on the intelligent feeding-system that discharges
CN114106988A (en) * 2012-10-26 2022-03-01 麻省理工学院 Humidity control in chemical reactors

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114106988A (en) * 2012-10-26 2022-03-01 麻省理工学院 Humidity control in chemical reactors
CN103382436A (en) * 2013-06-24 2013-11-06 江苏元洲生物工程有限公司 Continuous perfusion device of fermentation tank
CN107488584A (en) * 2017-09-25 2017-12-19 南京律智诚专利技术开发有限公司 One kind is based on the intelligent feeding-system that discharges

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Granted publication date: 20120905

Termination date: 20121215