CN202383143U - Antibody collaurum detection kit for helicobacter pylori - Google Patents
Antibody collaurum detection kit for helicobacter pylori Download PDFInfo
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- CN202383143U CN202383143U CN2011205044597U CN201120504459U CN202383143U CN 202383143 U CN202383143 U CN 202383143U CN 2011205044597 U CN2011205044597 U CN 2011205044597U CN 201120504459 U CN201120504459 U CN 201120504459U CN 202383143 U CN202383143 U CN 202383143U
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Abstract
The utility model discloses an antibody collaurum detection kit for helicobacter pylori, which comprises a kit cover, a kit base and a reagent strip. The kit cover is provided with a sample adding hole and a viewing port, the reagent strip is fixed onto the kit base, and the kit base is provided with clamping grooves or projections corresponding to the kit cover and can be fastened with the kit cover. The reagent strip comprises a plastic sheet, filtering sample paper, a glass fiber spacer, a nitrocellulose membrane and absorbent paper, wherein the filtering sample paper, the glass fiber spacer, the nitrocellulose membrane and the absorbent paper are fixed onto the plastic sheet and are sequentially connected, the glass fiber spacer is wrapped by collaurum labeled HP urase antigen, and the nitrocellulose membrane is provided with a detection line wrapped by HP urase antigen and HP cytotoxin protein antigen and a quality control line wrapped by an HP urase monoclonal antibody and an HP cytotoxin protein monoclonal antibody. The antibody collaurum detection kit for helicobacter pylori has the advantages of capability of realizing sensitive, peculiar and quick detection of the helicobacter pylori.
Description
Technical field
The utility model relates to a kind of detection and uses kit, is specifically related to a kind of stomach Helicobacter pylori antibody colloidal gold detection kit.
Background technology
Stomach Helicobacter pylori Helicobacter Pylori (being called for short HP) is a kind of spiral fashion gramnegative bacterium in the human stomach mucous membrane.This bacterium is the arch-criminal who causes chronic gastritis, gastric ulcer and duodenal ulcer even cancer of the stomach.
It is generally acknowledged that the CC that stomach Helicobacter pylori infects is such: the stomach Helicobacter pylori per os is settled down infection after arriving gastric mucosa; Cause chronic, superficial gastritis through several weeks or several months; Develop into duodenal ulcer, gastric ulcer, lymphocytic hyperplasia property gastric lymphoma, atrophic gastritis etc. behind several years or the many decades, and the latter is the factor that causes cancer of the stomach the most dangerous.Brainstrust thinks that stomach Helicobacter pylori infects and makes the danger that gets a cancer of the stomach increase 2.7-12 doubly, if there is not Helicobacter pylori infection, has at least the cancer of the stomach of 35%-89% can not take place.
Stomach Helicobacter pylori all has infection in the crowd of the different races in the world, different regions, can be described as chronic bacillary infection the most widely among the adult.Infection rate increases with the age and rises, and developing country is about 80%, and developed country is about 40%.20 years old-40 years old infection rate of China is 45.4%-63.6%, and the infection rate more than 70 years old is up to 78.9%.Therefore, whether exist HP antibody to infect in the detection human blood and have the very clinical meaning of reality for diagnosis HP.
The method that detects HP at present mainly contains:
(1) EUSA and western blot test: confirm the existence of HP in the human body through detecting HP antibody in the serum, this kind method specificity is high, and can carry out quantitative test, but can not differentiate previous infection and eqpidemic disease people at present.
(2) urea breath test and PCR detect test: the metabolin through measuring the urease that HP produces in vivo or the method for gene are confirmed the existence of HP; This kind method is highly sensitive; But complicated operation, apparatus expensive are inappropriate for basic unit's testing, have promoted the use of limitation.
(3) microbe growth and other pathology detection methods etc., these methods also generally have advantage highly sensitive, high specificity, but also exist to cultivate and the testing conditions of pathology method is strict, complicated operation, the limitation that needs the professional to operate.
So; Be badly in need of at present a kind of quick, accurate, highly sensitive, the method or reagent that just can detect HP without any need for main equipment; This kind method or reagent help stomach Helicobacter pylori and detect in the popularizing and applying of basic unit, and chronic gastritis, gastric ulcer and duodenal ulcer even Prevention and Treatment of Stomach Cancer that stomach Helicobacter pylori is caused have great clinical meaning.
The utility model content
The purpose of the utility model is to overcome the defective of prior art, and a kind of stomach Helicobacter pylori antibody colloidal gold detection kit is provided, and realizes the sensitivity of stomach Helicobacter pylori (HP), special, detection fast.
The technical scheme of the utility model is: a kind of stomach Helicobacter pylori antibody colloidal gold detection kit; Comprise lid, cassette holder and reagent strip; Said lid is provided with well and observation panel; Be fixed with said test strips on the said cassette holder; Said cassette holder is provided with draw-in groove corresponding with lid or projection, and cassette holder can fasten with lid, and said reagent strip comprises plastic tab and is fixed in filter appearance paper, spun glass pad, nitrocellulose filter and the thieving paper that is connected successively end to end on the said plastic tab; Be coated with the HP urease antigen of colloid gold label on the said spun glass pad; Said nitrocellulose filter is provided with the detection line that is coated with HP urease antigen and HP cytotoxin proteantigen and is coated with anti-HP urease monoclonal antibody and the nature controlling line of anti-HP cytotoxin protein monoclonal antibody.
More excellent, said detection line and nature controlling line are arranged in parallel, and said detection line is near the spun glass pad, and said nature controlling line is near thieving paper.
More excellent, said well is circular, said observation panel is oval; More excellent, said well and observation panel are choana, choana suitable for reading big, following young.
More excellent, the corresponding well of said filter appearance paper, the observation panel on the corresponding lid of said nitrocellulose filter, and the detection line on the said nitrocellulose filter is with nature controlling line is exposed to or partly be exposed to said observation panel.The mark " T " of corresponding observation panel one side of said detection line, the mark " C " of corresponding observation panel one side of nature controlling line.
More excellent, the live width of said detection line and said nature controlling line is 0.7mm.
During use, drip 1-2 in sample application zone and drip (about 50-80 μ 1) whole blood or serum, drip 2-3 again and drip damping fluid, sentence read result after 10-15 minute after dripping sample.If contain the antibody of stomach Helicobacter pylori in the blood; It will combine to form HP urease antibody-HP urease antigen-colloidal gold composite and HP cytotoxin enzyme antibody-HP cytotoxin enzyme antigen-colloidal gold composite with HP urease antigen-colloidal gold composite and the HP cytotoxin enzyme antigen-colloidal gold composite on being fixed on the plain film of spun glass; Through the capillary action chromatography to nitrocellulose filter; Combine with antigen on the detection line on the nitrocellulose filter and the antiantibody on the nature controlling line; Capture colloid gold particle and present red ribbon, positive result.If there is not HP antigen in the urine sample; Then the HP urease antigen-colloidal gold composite on the plain film of spun glass and HP cytotoxin enzyme antigen-colloidal gold composite only with nature controlling line on anti-HP urease monoclonal antibody and anti-HP cytotoxin protein monoclonal antibody combine; Nature controlling line captures colloid gold particle and presents red ribbon, negative result.
Negative: as a colour band to occur in the watch window as a result.Be that red lines appear in nature controlling line (C line) position.No stomach Helicobacter pylori antibody exists in the expression sample.
Positive: as two colour bands to occur in the watch window as a result.Be that red lines respectively appear in detection zone (T line) and nature controlling line (C line) position, have stomach Helicobacter pylori antibody to exist in the expression sample.
Invalid: nature controlling line (C line) does not occur.In any case, the C line all should form, and the expression application of sample is correct with operation.The C line does not occur showing that test result is uncertain, should reform.
The stomach Helicobacter pylori antibody colloidal gold detection kit susceptibility height of the utility model, high specificity, easy and simple to handle saving time very are fit to on-the-spot the use, and HP is detected.
Description of drawings
Fig. 1: stomach Helicobacter pylori antibody assay kit (1. cassette holder 2. lids 3. reagent strips 4. wells 5. observation panels)
Fig. 2: the stomach Helicobacter pylori antibody test agent box (1. cassette holder 3. reagent strips 6. filter appearance paper 7. spun glass pads 8. nitrocellulose filters 9. thieving papers 10. detection lines 11. nature controlling lines 13. draw-in grooves) that removes lid
Fig. 3: the reagent strip schematic side view of stomach Helicobacter pylori antibody assay kit (6. filter appearance paper 7. spun glass pads 8. nitrocellulose filters 9. thieving papers 12. plastic tabs)
Embodiment
Stomach Helicobacter pylori antibody colloidal gold detection kit shown in Fig. 1-3 comprises lid 2, cassette holder 1 and reagent strip 3; Lid 2 is provided with well 4 and observation panel 5, is fixed with test strips 3 on the cassette holder 1, and cassette holder 1 is provided with draw-in groove 13, the projection at corresponding lid 2 back sides, and cassette holder 1 can fasten with lid 2; Reagent strip 3 comprises plastic tab 12 and is fixed in filter appearance paper 6, spun glass pad 7, nitrocellulose filter 8 and the thieving paper 9 that is connected successively end to end on the plastic tab 12; Be coated with the HP urease antigen of colloid gold label on the spun glass pad 7; Nitrocellulose filter 8 is provided with the detection line 10 that is coated with HP urease antigen and HP cytotoxin proteantigen and is coated with anti-HP urease monoclonal antibody and the nature controlling line 11 of anti-HP cytotoxin protein monoclonal antibody.Well 4 on the lid 2 is circular, is positioned at lid 2 one ends, is used for adding testing sample, and circular well is choana, and is suitable for reading big, following young, can prevent effectively that sample from overflowing.The middle part of lid 2 has oval observation panel 5, and oval observation panel 5 also is a choana, and is suitable for reading big, following young.
On the nitrocellulose filter 8, detection line 10 is arranged in parallel with nature controlling line 11, and detection line 10 is near spun glass pad 7, and nature controlling line 11 is near thieving paper 9.Well 4 on the filter appearance paper 6 corresponding lids 2 on the reagent strip 3; Observation panel 5 on the nitrocellulose filter 8 corresponding lids 2; And the detection line on the nitrocellulose filter 8 10 is exposed to observation panel 5 with nature controlling line 11 parts, and " T " of observation panel 5 one sides on the detection line 10 corresponding lids 2, on the nature controlling line 11 corresponding lids 2 " C " of observation panel 5 one sides; The live width of detection line and nature controlling line is 0.7mm, and the reagent on detection line and the nature controlling line all adopts the specking mode to be fixed on above the reagent strip.
Claims (6)
1. stomach Helicobacter pylori antibody colloidal gold detection kit; Comprise lid, cassette holder and reagent strip, said lid is provided with well and observation panel, is fixed with said test strips on the said cassette holder; Said cassette holder is provided with draw-in groove corresponding with lid or projection; Cassette holder can fasten with lid, it is characterized in that, said reagent strip comprises plastic tab and is fixed in filter appearance paper, spun glass pad, nitrocellulose filter and the thieving paper that is connected successively end to end on the said plastic tab; Be coated with the HP urease antigen of colloid gold label on the said spun glass pad; Said nitrocellulose filter is provided with the detection line that is coated with HP urease antigen and HP cytotoxin proteantigen and is coated with anti-HP urease monoclonal antibody and the nature controlling line of anti-HP cytotoxin protein monoclonal antibody.
2. kit as claimed in claim 1 is characterized in that said detection line and nature controlling line are arranged in parallel, and said detection line is near the spun glass pad, and said nature controlling line is near thieving paper.
3. kit as claimed in claim 1 is characterized in that, said well is circular, and said observation panel is oval.
4. kit as claimed in claim 1 is characterized in that, said well and observation panel are choana, choana suitable for reading big, following young.
5. kit as claimed in claim 1; It is characterized in that; Well on the corresponding lid of said filter appearance paper, the observation panel on the corresponding lid of said nitrocellulose filter, and the detection line on the said nitrocellulose filter is with nature controlling line is exposed to or partly be exposed to said observation panel.
6. kit as claimed in claim 1 is characterized in that, the live width of said detection line and said nature controlling line is 0.7mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011205044597U CN202383143U (en) | 2011-12-07 | 2011-12-07 | Antibody collaurum detection kit for helicobacter pylori |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011205044597U CN202383143U (en) | 2011-12-07 | 2011-12-07 | Antibody collaurum detection kit for helicobacter pylori |
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| Publication Number | Publication Date |
|---|---|
| CN202383143U true CN202383143U (en) | 2012-08-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011205044597U Expired - Lifetime CN202383143U (en) | 2011-12-07 | 2011-12-07 | Antibody collaurum detection kit for helicobacter pylori |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202383143U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111426824A (en) * | 2020-03-31 | 2020-07-17 | 广东药科大学附属第一医院 | Colloidal gold test paper and preparation method and application thereof |
| CN111983229A (en) * | 2020-09-07 | 2020-11-24 | 三门县人民医院 | Reagent strip for quantitatively detecting helicobacter pylori antibody by colloidal gold and detection method |
| CN112816696A (en) * | 2019-11-15 | 2021-05-18 | 北京康美天鸿生物科技有限公司 | Kit for detecting human urine helicobacter pylori antibody by colloidal gold method and preparation method thereof |
| CN116068170A (en) * | 2022-09-28 | 2023-05-05 | 北京金沃夫生物工程科技有限公司 | Test paper and kit for detecting helicobacter pylori |
-
2011
- 2011-12-07 CN CN2011205044597U patent/CN202383143U/en not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112816696A (en) * | 2019-11-15 | 2021-05-18 | 北京康美天鸿生物科技有限公司 | Kit for detecting human urine helicobacter pylori antibody by colloidal gold method and preparation method thereof |
| CN111426824A (en) * | 2020-03-31 | 2020-07-17 | 广东药科大学附属第一医院 | Colloidal gold test paper and preparation method and application thereof |
| CN111983229A (en) * | 2020-09-07 | 2020-11-24 | 三门县人民医院 | Reagent strip for quantitatively detecting helicobacter pylori antibody by colloidal gold and detection method |
| WO2022048693A1 (en) * | 2020-09-07 | 2022-03-10 | 三门县人民医院 | Reagent strip for quantitatively detecting helicobacter pylori antibody by means of colloidal gold and detection method |
| CN116068170A (en) * | 2022-09-28 | 2023-05-05 | 北京金沃夫生物工程科技有限公司 | Test paper and kit for detecting helicobacter pylori |
| CN116068170B (en) * | 2022-09-28 | 2023-09-15 | 北京金沃夫生物工程科技有限公司 | Test paper and kit for detecting Helicobacter pylori |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20120815 |
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| CX01 | Expiry of patent term |