CN202329577U - In-vivo measurement device for leaf area of broad-leaved plant - Google Patents
In-vivo measurement device for leaf area of broad-leaved plant Download PDFInfo
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- CN202329577U CN202329577U CN2011203836093U CN201120383609U CN202329577U CN 202329577 U CN202329577 U CN 202329577U CN 2011203836093 U CN2011203836093 U CN 2011203836093U CN 201120383609 U CN201120383609 U CN 201120383609U CN 202329577 U CN202329577 U CN 202329577U
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- blade
- area
- leaf
- leaf area
- cover plate
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Abstract
An in-vivo measurement device for the leaf area of broad-leaved plant is disclosed. The device comprises a bottom plate and a cover plate, wherein one edge of each of the bottom plate and the cover plate is connected with the other to form an area with a hinge-type structure and a single colour; the colour of the area has a contrast with the colour of the to-be-measured leaf; and the cover plate is made from a transparent material. The problems of damage on the measured plant leaf, too expensive measurement instrument, too cockamamie measurement method, limited measurement site and the like existing in the present measurement technology can be efficiently overcome by utilizing the device.
Description
Technical field
The utility model relates to the botany test apparatus, particularly relates to a kind of broad leaf plant leaf area living body measurement apparatus.
Background technology
Leaf is the nutrition organs of higher plant; It contains chloroplast; It is the major organs that green plants carries out photosynthesis and transpiration; Blade is the main part of leaf, is generally a very thin bluff body, helps that light penetrates the tissue of leaf and absorbing light, the carbon dioxide of maximum area carries out photosynthesis.Growth and the leaf area of plant are closely related; The size of leaf area has influenced the photosensitizing substance accumulation of plant on the one hand; On the other hand the growth of plant again the variation through leaf area obtain embodying, the leaf area of therefore measuring plant has great importance for growth physiology, photosynthetic physiology and the stress physiology of plant.
The mensuration of plant blade area has many methods and instrument, and leaf area assay method at present both at home and abroad commonly used has several square methods, paper-cut method, scanning method, leaf area appearance method, coefficient determination method and the camera method etc. of taking pictures.
The number square method only needs the standard square paper, and method simple, intuitive, expense are very low, but must drop into a large amount of manual works, on counting, receives artificial factor bigger, and very low for measuring blade precision in irregular shape, can't measure even; The paper-cut method is also comparatively simple and convenient, but also can't measure the very irregular and less blade of area of shape, in addition because the influence of the factors such as homogeneity of the artificial subjectivity and the paper that adopts also can't guarantee its degree of accuracy of measuring the result that draws; Scanning method is to use a kind of digital image processing techniques comparatively general and that efficient is higher at present, but needs computer, scanner and image processing software, and expense is higher relatively; And operated site limitation; Need measure in the laboratory, pluck blade to measure during this period of time during, blade is difficult for preserving and also keeps original state; And when leaf water content is big, is difficult for paving and very easily damages scanner; Leaf area appearance method can be measured leaf area fast and accurately; Simple to operate, can carry out analyses such as plant leaf blade damaged, variable color or morbid state, but when measuring; The blade sweep velocity is desirable not to the utmost; And instrument be external import mostly, and price is costliness too, is unwell to common scientific research Application Research; The coefficient determination method can be used for the living body measurement of blade, but need carry out the mensuration of coefficient respectively for plant leaf blades not of the same race, different leaf ages, and determination efficiency is very low; The camera method of taking pictures is that the cardboard with a blade and a fixed-area is placed on same plane and takes pictures, and washes out the papery photo then, through cutting blade-section and cardboard part; And weigh and then try to achieve blade area through balance, this method can be measured the live body blade, and is little to blade injury; But too loaded down with trivial details, when cutting blade photo, be difficult to handle for edge or the too complicated blade of shape; If to same blade replication, the manual work of input is also more, it is also accurate not to the utmost to measure the result.
This shows, at present more general leaf area assay method, all exist defective.For the leaf area assay method that exsomatizes; All need picking blade so just can't be carried out continuous observation to same leaf growth situation of change, also can affect the growth of plant; Pluck measure this during, the preservation of blade also exists certain problem.And for the leaf area assay method of live body, exist instrument and equipment to cost an arm and a leg, be difficult to popularization and application for common scientific research, or method is too loaded down with trivial details, need to drop into more manual work and the not high problem of degree of accuracy.
The utility model content
The utility model proposes a kind of broad leaf plant leaf area living body measurement apparatus for addressing the above problem.
The technical scheme of the utility model is: a kind of broad leaf plant leaf area living body measurement apparatus; Comprise base plate and cover plate; One of them edge of base plate and cover plate is joined together to form hinge typed structure; It is characterized in that: monochromatic areas, its color and blade color to be measured have contrast, and described cover plate is that transparent material is processed.
Described monochromatic areas is a rectangle, and its color is a white.
The edge of described base plate and cover plate is through the adhesive plaster bonding.
The blade size that can will measure according to actual institute, the ratio and the size of each parts of adjustment instrument, the instrument of making different size is to adapt to the needs in the practical application.
The beneficial effect of the utility model:
1. overcome the restriction of measuring the place, can directly measure in the open air.
2. can simple and effective mensuration blade area, and need not to pluck blade, plant strain growth is not had influence, and can carry out long-term observation in the growth change in difference growth season same blade.
3. cost is low, and speed is fast, needs equipment few, and is simple to operate, is directed to the blade that varies in size, and can make and adopt the blade measuring device of different size, guarantees the degree of accuracy of data.
4. too can fast measuring for complex-shaped blade such as out-of-shape and edge sawtooth, and in mensuration, can avoid interference from human factor as much as possible.
Therefore take all factors into consideration, the utility model has very practical value for scientific research, for the scientific research of subjects such as farming, woods, botany, ecology provides a kind of new basic data collection scheme, is suitable for promoting the use of.
Description of drawings
Fig. 1 is the overall diagram of broad leaf plant leaf area living body measurement apparatus after opening;
Among the figure: 1, cover plate; 2, base plate; 3, monochromatic areas; 4, adhesive plaster.
Embodiment
Embodiment: referring to Fig. 1; A kind of broad leaf plant leaf area living body measurement apparatus among the figure; Comprise base plate and cover plate, one of them edge of base plate and cover plate is joined together to form hinge typed structure, it is characterized in that: monochromatic areas; Its color and blade color to be measured have contrast, and described cover plate is that transparent material is processed.Described monochromatic areas is a rectangle, and its color is a white.The edge of described base plate and cover plate is through the adhesive plaster bonding.The blade size that can will measure according to actual institute, the ratio and the size of each parts of adjustment instrument, the instrument of making different size is to adapt to the needs in the practical application.
Utilize said apparatus to carry out the method for broad leaf plant leaf area living body measurement,
1.. the base plate of described broad leaf plant leaf area living body measurement apparatus is positioned under the live body blade that needs to measure, and guarantees that blade all is positioned at its monochromatic areas.
2.. the transparent cover plate that fixed blade is used covers to clamp blade, guarantees leveling blade corrugationless, folding.
3.. use digital camera and take pictures for the leaf area determinator,, can repeat to take pictures repeatedly for guaranteeing degree of accuracy.
4.. utilize data line to import in the computer in the photo that photographed.
5.. use the Photoshop software of Adobe company, the logarithmic code photo carries out pre-service, eliminates the deformation of taking pictures.
6.. the Photoshop software logarithmic code photo of using Adobe company is analyzed; The applied magnetic lasso tool obtains the pixel number N1 that blade-section contains; Use magic wand menu and obtain being covered the remaining monochromatic areas pixel number N2 in back by blade, then (N1+N2) is the pixel number that the colour prime white rectangular area of leaf area determinator is contained.
7.. the area S (unit: cm of the white portion that sets during by initial making leaf area determinator
2), can obtain area A=S * N1/ (N1+N2) (unit: cm of the blade that will measure
2).
8.. repeat 5. above-mentioned～7. repeatedly, the photo that obtains that repeats to take pictures is measured one by one, averaging is the leaf area of this blade.
The detailed method of operation of above-mentioned steps is following:
1. the blade wiped clean that will measure, the surface dust dust and other impurities above removing, the each several part explanation of consulting accompanying drawing 1.Leaf area determinator base plate is positioned under the live body blade that needs to measure, and guarantees that blade-section all is positioned at device white rectangle zone.
2. the thin transparent plastic cover plate of fixed blade being used covers to be used for clamping blade, guarantees that the blade corrugationless is overlapping.
3. open the macro mode of digital camera; Transfer to photo resolution the highest; 15cm-45cm adjusts focal length in the place directly over the transparent plastic cover plate; Guarantee that all white portions are positioned at camera lens central authorities as far as possible and are included in photo, blade is taken, and noted photo numbering and its corresponding blade code.When taking, needing to note ambient light sufficient, avoid the direct projection of sunlight as far as possible, close flashlamp, shade and inverted image occur, influence the treatment progress in later stage to prevent photo.When taking pictures, camera body need be tried one's best and installed parallelly, avoids photo excessively distortion to occur.This process can repeat repeatedly, measures degree of accuracy to improve.If (leaf position is lower, can base plate be placed the blade below, holds digital camera and takes from the top; Higher like leaf position, then can take vacuum side of blade with digital camera from the below with base plate as for the blade top, can measure blade area equally).
4. utilize data line that the photo that photographs is imported computer.
5. open the Photoshop software of Adobe company, what adopt in this example is Photoshop CS5 version.Click " file (F) " → " opening (O) " → " photo that selection will be handled " → " opening (O) " successively, be about to the photo that will measure in Photoshop, open.
5. menu bar is clicked " filter (T) " → " lens correction (R) " up, perhaps directly presses shortcut " Ctrl+Shiff+R ", accesses the lens correction window.Menu bar is selected " making by oneself " Shipping Options Page on the right side, at " angle (A) " numerical value the photo angle is aligned; Pulling " vertical perspective (R) " then, " Hrizontal perspective (O) " down the adjusting button or directly import relevant numerical thereafter with the elimination distortion that the camera lens out of plumb brings of taking pictures; Adjusting button under the pulling " removing distortion (V) " or direct input value are to eliminate the spherical deformation that digital camera lens brings; Click at last and confirm to obtain gratifying photo pretreating effect.
6. toolbar is clicked " lasso tool " by right key and is launched menu selection " Magnetic Lasso Tool " in the program left side; Arbitrary position at blade edge is clicked, and to build initial point, rolling mouse moves along blade edge then; Lasso trick can be delineated out leaf area automatically; Finally get back to starting point, this moment, a circle appearred in the Magnetic Lasso Tool icon lower right corner, and click can obtain the leaf area of complete closure; Click the top menu and " analyze (A) " → " record is measured (M) ", the area value that the pop-up window label " measures 1 " is the pixel number N1 of the rectangular area area of choosing.Can find out the N1=1846589.0 of accompanying drawing 2.
7. in the white portion click right, select " cancellation is selected " menu; The toolbar in the left side is clicked " quick selection tool " by right key, selects " magic wand menu ", and left button is clicked in the white portion optional position; Click the top menu and " analyze (A) " → " record is measured (M) "; The area value that the pop-up window label " measures 2 " is the pixel number N2=4821139.0 of the white portion of choosing, and then the total pixel number in the white rectangle of blade area determinator zone is N1+N2=6667728.0.
8. area S=La * Lb (unit: cm of the white portion that sets during by initial making leaf area determinator
2), can obtain the area A=((unit: cm of La * Lb * N1)/(N1+N2) of the blade that will measure
2).In this example, leaf area A=(15.0 * 15.0 * 1846589.0)/6667728.0=62.31cm
2In practical application, the leaf area (this step can be calculated all data recording to EXCLE) that can be this blade with averaging behind each replication.
Through revision test repeatedly, the error of the leaf area that the apparatus and method of application the utility model are surveyed can remain on below 1%, explains that the utility model is practical reliable, can apply.
The above; Be merely the preferable embodiment of the utility model; But the protection domain of the utility model is not limited thereto; Any technician who is familiar with the present technique field is in the technical scope that the utility model discloses, and the variation that can expect easily or replacement all should be encompassed within the protection domain of the utility model.
Claims (3)
1. broad leaf plant leaf area living body measurement apparatus; Comprise base plate and cover plate; One of them edge of base plate and cover plate is joined together to form hinge typed structure; It is characterized in that: described base plate is provided with the monochromatic areas that area is confirmed, its color and blade color to be measured have contrast, and described cover plate is that transparent material is processed.
2. broad leaf plant leaf area living body measurement apparatus according to claim 1 is characterized in that: described monochromatic areas is a rectangle, and its color is a white.
3. broad leaf plant leaf area living body measurement apparatus according to claim 1 is characterized in that: the edge of described base plate and cover plate is through the adhesive plaster bonding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011203836093U CN202329577U (en) | 2011-10-11 | 2011-10-11 | In-vivo measurement device for leaf area of broad-leaved plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011203836093U CN202329577U (en) | 2011-10-11 | 2011-10-11 | In-vivo measurement device for leaf area of broad-leaved plant |
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| Publication Number | Publication Date |
|---|---|
| CN202329577U true CN202329577U (en) | 2012-07-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011203836093U Expired - Fee Related CN202329577U (en) | 2011-10-11 | 2011-10-11 | In-vivo measurement device for leaf area of broad-leaved plant |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103234487A (en) * | 2013-03-29 | 2013-08-07 | 吉林大学 | Method for measuring blade area and blade surface scab area of plants |
| CN103308025A (en) * | 2013-06-05 | 2013-09-18 | 福建省农业科学院茶叶研究所 | Plant leaf area print weighing in-vivo testing method |
| WO2014100856A1 (en) * | 2012-12-24 | 2014-07-03 | University Of Technology, Sydney | An image processing based method to estimate crop requirements for nutrient fertiliser |
| CN105571532A (en) * | 2016-01-01 | 2016-05-11 | 三峡大学 | Leaf area measuring method |
| CN108120374A (en) * | 2017-11-30 | 2018-06-05 | 甘肃省治沙研究所 | Plant leaf area data acquisition blade presss from both sides and the lossless computation of plant blade area |
| CN113624165A (en) * | 2021-08-09 | 2021-11-09 | 海南青峰生物科技有限公司 | Rice leaf area measuring device and method based on 5G and image recognition |
| RU216944U1 (en) * | 2022-06-08 | 2023-03-10 | Сергей Александрович Набатников | Device for determining the leaf area of plants |
-
2011
- 2011-10-11 CN CN2011203836093U patent/CN202329577U/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014100856A1 (en) * | 2012-12-24 | 2014-07-03 | University Of Technology, Sydney | An image processing based method to estimate crop requirements for nutrient fertiliser |
| CN103234487A (en) * | 2013-03-29 | 2013-08-07 | 吉林大学 | Method for measuring blade area and blade surface scab area of plants |
| CN103308025A (en) * | 2013-06-05 | 2013-09-18 | 福建省农业科学院茶叶研究所 | Plant leaf area print weighing in-vivo testing method |
| CN105571532A (en) * | 2016-01-01 | 2016-05-11 | 三峡大学 | Leaf area measuring method |
| CN108120374A (en) * | 2017-11-30 | 2018-06-05 | 甘肃省治沙研究所 | Plant leaf area data acquisition blade presss from both sides and the lossless computation of plant blade area |
| CN113624165A (en) * | 2021-08-09 | 2021-11-09 | 海南青峰生物科技有限公司 | Rice leaf area measuring device and method based on 5G and image recognition |
| RU216944U1 (en) * | 2022-06-08 | 2023-03-10 | Сергей Александрович Набатников | Device for determining the leaf area of plants |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120711 Termination date: 20141011 |
|
| EXPY | Termination of patent right or utility model |