CN202305981U - Enhanced slide-block type confocal optical scanning instrument with micro-lenses - Google Patents

Enhanced slide-block type confocal optical scanning instrument with micro-lenses Download PDF

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Publication number
CN202305981U
CN202305981U CN2011202928365U CN201120292836U CN202305981U CN 202305981 U CN202305981 U CN 202305981U CN 2011202928365 U CN2011202928365 U CN 2011202928365U CN 201120292836 U CN201120292836 U CN 201120292836U CN 202305981 U CN202305981 U CN 202305981U
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slide block
light
micro
microlens array
pin hole
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CN2011202928365U
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Chinese (zh)
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赖博
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Abstract

The utility model relates to an enhanced slide-block type confocal optical scanning instrument with micro-lenses, in particular to a slide-block type confocal optical scanning instrument which utilizes a CCD (Charge Coupled Device), an EMCCD ((Electron Multiplying Charge Coupled Device), a CMOS (Complementary Metal Oxide Semiconductor), and other area-array detectors to serve as detectors. The optical scanning instrument is mainly characterized in that a micro-lens array and a light-proof slide block with a plurality of light-transmitting needle holes are driven to slide to scan samples in a reciprocating manner at a constant speed; the light-proof slide block with the plurality of light-transmitting needle holes is positioned on the focal plane of the micro-lens array; the micro-lenses on the micro-lens array and the needle holes on the slide block are in one-to-one correspondence; and the focus point of each micro-lens is superposed with the center of the corresponding needle hole. The optical scanning instrument has the advantages of high illumination efficiency, low stray light background noise, easiness in synchronization with camera exposure control, uniform lighting strength, and the like.

Description

A kind of micro-lens enhancement type slide block type confocal optical scanner
Technical field
The utility model relates to a kind of micro-lens enhancement type slide block type confocal optical scanner, specifically is to use the slide block type confocal optical scanner of planar array detectors such as CCD, EMCCD and CMOS as detecting device.
Background technology
At present, existing slide block type confocal scanning appearance mainly is made up of with lens combination the light tight single sliding block of arranging a plurality of printing opacity pin holes or double-slider, during work through motor-driven slide block to-and-fro movement realization scanning imagery.For fear of the interference between the adjacent pin hole, improve the image quality of scanner, pin hole in some way and distance be arranged on the slide block.Because the area of pin hole has only 2% of whole slide block area, causes the utilization ratio of light source also to have only 2%.Therefore, cause the not enough and high problem of slide block reflect stray light noise of illumination intensity of scanner.
Summary of the invention
The utility model solves the technical scheme that its technical matters adopted: between light source and slide block; Place a microlens array; Lenticule in the microlens array is corresponding one by one with pin hole; Make the light of light emitted pass through each pin hole on the slide block, be radiated on the sample then through beam splitting behind the microlens array, focusing.Because lenticule is to the focussing force of light, the luminous flux that passes pin hole is improved, and has therefore improved the utilization ratio of light source; And reduced the parasitic light that slide block reflects simultaneously, reduce the parasitic light noise of scanner.
The utility model is as shown in Figure 1 to be comprised: light source 1; Illumination color filter 2; Illuminating lens group 3; Microlens array 4 has been arranged a plurality of lenticules 5 on it; Slider bracket 6; Drive motor 7; Dichroic beam splitter 8; Lighttight slide block 9 is being arranged a plurality of printing opacity pin holes 10 on it; Imaging lens group 11; Imaging color filter 12; Controller 13.
The purpose of the utility model realizes through following technical scheme: as shown in Figure 2, between illuminating lens 3 and light tight slide block 9, place microlens array 4 perpendicular to light path; The light of light source 1 emission arrives microlens array 4 by beam splitting behind over-illumination color filter 2 and illuminating lens 3, focused on respectively through the printing opacity pin hole 10 on the light tight slide block 9 by the light of beam splitting; Light tight slide block 9 is positioned on the focal plane of microlens array 4; Printing opacity pin hole 10 on the light tight slide block 9 is corresponding one by one with the lenticule 5 of microlens array 4, and the focus of the center of pin hole 10 and lenticule 5 overlaps.
The beneficial effect of the utility model is:
1, improves the utilization ratio of light source, strengthened the illumination level of sample.
2, reduce the parasitic light of slide block reflection, reduced ground unrest.
Description of drawings
Fig. 1: structural representation of the present invention
Fig. 2: lenticule of the present invention and pin hole synoptic diagram
Fig. 3: the synoptic diagram of first kind of embodiment of the present invention
Fig. 4: the synoptic diagram of second kind of embodiment of the present invention
Fig. 5: the synoptic diagram of the third embodiment of the present invention
The drawing explanation:
Light source 1; Illumination color filter 2; Illuminating lens group 3; Microlens array 4 has been arranged a plurality of lenticules 5 on it; Slider bracket 6; Drive motor 7; Dichroic beam splitter 8; Lighttight slide block 9 is being arranged a plurality of printing opacity pin holes 10 on it; Imaging lens group 11; Imaging color filter 12; Controller 13; Micro objective 14; Sample 15; Monitor 16; Computing machine 17; Display 18.
Specific embodiment
Further describe the utility model below in conjunction with accompanying drawing and embodiment.
Embodiment 1
Fig. 3 is first kind of micro-lens enhancement type slide block type confocal optical scanner synoptic diagram relevant with the utility model:
In the present embodiment; The illumination light of light source 1 emission is passed pin hole 10 and the micro objective 14 on lenticule 5 on the illumination color filter 2 placed successively in the light path, illuminating lens group 3, the microlens array 4, dichroic beam splitter 8, the light tight slide block 9; Impinge upon on the sample 15 of micro objective 14 imaging focal plane, wherein have only the illumination light of specific wavelength can pass illumination color filter 2.Illuminated sample 15 emitted fluorescence are returned the pin hole 10 that passes on micro objective 14 and the light tight slide block 9; Passed imaging lens group 11 by dichroic beam splitter 8 reflections and arrive detecting devices 16, and the illumination light of sample reflection can not see through imaging color filter 12 arrival detecting devices 16 with the color filter 12 that forms images.Counter 17 (not shown)s that are connected with detecting device 16 are stored related data or are passed through display 18 (not shown) display images.
Embodiment 2
Fig. 4 is second kind of micro-lens enhancement type slide block type confocal optical scanner synoptic diagram relevant with the utility model:
In the present embodiment; The illumination light of light source 1 emission is passed lenticule 5, the pin hole 10 on the light tight slide block 9 and the micro objective 14 on the illumination color filter 2 placed successively in the light path, illuminating lens group 3, dichroic beam splitter 8, the microlens array 4; Impinge upon on the sample 15 of micro objective 14 imaging focal plane, wherein have only the illumination light of specific wavelength can pass illumination color filter 2.Illuminated sample 15 emitted fluorescence are returned the pin hole 10 that passes on micro objective 14, the light tight slide block 9 and the lenticule 5 on the microlens array 4; Passed imaging lens group 11 by dichroic beam splitter 8 reflections and arrive detecting devices 16, and the illumination light of sample reflection can not see through imaging color filter 12 arrival detecting devices 16 with the color filter 12 that forms images.Counter 17 (not shown)s that are connected with detecting device 16 are stored related data or are passed through display 18 (not shown) display images.
Embodiment 3
Fig. 5 is the third micro-lens enhancement type slide block type confocal optical scanner synoptic diagram relevant with the utility model:
In the present embodiment; The illumination light of light source 1 emission is passed the lenticule 5 on the illumination color filter 2 placed successively in the light path, illuminating lens group 3, the microlens array 4; Passed the pin hole 10 and micro objective 14 on the light tight slide block 9 again after dichroic beam splitter 8 reflections; Impinge upon on the sample 15 of micro objective 14 imaging focal plane, wherein have only the illumination light of specific wavelength can pass illumination color filter 2.Illuminated sample 15 emitted fluorescence return the pin hole 10, the dichroic beam splitter 8 that pass on micro objective 14, the light tight slide block 9, pass imaging lens group 11 and imaging color filter 12 arrives detecting devices 16, and the illumination light of sample reflection can not see through imaging color filter 12 and arrives detecting devices 16.Counter 17 (not shown)s that are connected with detecting device 16 are stored related data or are passed through display 18 (not shown) display images.

Claims (8)

1. micro-lens enhancement type slide block type confocal optical scanner; Reciprocatingly slide come scanning samples with the light tight slide block of having arranged a plurality of printing opacity pin holes with the constant speed of setting through driving microlens array simultaneously, it is characterized in that the lenticule of said microlens array is corresponding one by one with pin hole on the said light tight slide block.
2. micro-lens enhancement type slide block type confocal optical scanner according to claim 1 is characterized in that, the said light tight slide block of having arranged a plurality of printing opacity pin holes is positioned on the focal plane of said microlens array.
3. micro-lens enhancement type slide block type confocal optical scanner according to claim 1 and 2 is characterized in that, the center of the pin hole on the lenticular focus of said microlens array and the said light tight slide block overlaps one by one.
4. micro-lens enhancement type slide block type confocal optical scanner according to claim 1 and 2 is characterized in that, said microlens array is made up of in Fresnel lens, secondary curved reflector, the miniature lenticular lens type lenticule one or more.
5. one kind reciprocatingly slides scanning samples to reciprocatingly slide the micro-lens enhancement type slide block type confocal optical scanner of scanning samples with the constant speed of setting through driving microlens array and the light tight slide block of having arranged a plurality of printing opacity pin holes simultaneously with the constant speed of setting, by forming with the lower part:
Light source (1);
Arranged on microlens array (4) and its of lenticule (5) and arranged the lighttight slide block of a plurality of printing opacity pin holes (10) remainder (9);
Driving said microlens array (4) and said light tight slide block (9) slides with the constant speed of setting; The light that makes said light source (1) emission is through said lenticule (5) and pin hole (10), intactly, slider bracket (6), drive motor (7) and the controller (13) of uniform scanning samples;
Form by dichroic beam splitter (8), imaging lens group (11) and imaging color filter (12), make light that illuminated sample reflects or emitted fluorescence pass collateral branch's light path of arrival face battle array monitor behind the said pin hole on the said imaging slide block.
6. a kind of micro-lens enhancement type slide block type confocal optical scanner according to claim 1 and 2, also by forming with the lower part:
Light source (1);
Driving said microlens array (4) and said light tight slide block (9) slides with the constant speed of setting; The light that makes said light source (1) emission is through said lenticule (5) and pin hole (10), intactly, slider bracket (6), drive motor (7) and the controller (13) of uniform scanning samples;
Form by dichroic beam splitter (8), imaging lens group (11) and imaging color filter (12), make light that illuminated sample reflects or emitted fluorescence pass collateral branch's light path of arrival face battle array monitor behind the said pin hole on the said imaging slide block.
7. micro-lens enhancement type slide block type confocal optical scanner according to claim 3, also by forming with the lower part:
Light source (1);
Driving said microlens array (4) and said light tight slide block (9) slides with the constant speed of setting; The light that makes said light source (1) emission is through said lenticule (5) and pin hole (10), intactly, slider bracket (6), drive motor (7) and the controller (13) of uniform scanning samples;
Form by dichroic beam splitter (8), imaging lens group (11) and imaging color filter (12), make light that illuminated sample reflects or emitted fluorescence pass collateral branch's light path of arrival face battle array monitor behind the said pin hole on the said imaging slide block.
8. micro-lens enhancement type slide block type confocal optical scanner according to claim 4, also by forming with the lower part:
Light source (1);
Driving said microlens array (4) and said light tight slide block (9) slides with the constant speed of setting; The light that makes said light source (1) emission is through said lenticule (5) and pin hole (10), intactly, slider bracket (6), drive motor (7) and the controller (13) of uniform scanning samples;
Form by dichroic beam splitter (8), imaging lens group (11) and imaging color filter (12), make light that illuminated sample reflects or emitted fluorescence pass collateral branch's light path of arrival face battle array monitor behind the said pin hole on the said imaging slide block.
CN2011202928365U 2011-08-12 2011-08-12 Enhanced slide-block type confocal optical scanning instrument with micro-lenses Expired - Lifetime CN202305981U (en)

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CN2011202928365U CN202305981U (en) 2011-08-12 2011-08-12 Enhanced slide-block type confocal optical scanning instrument with micro-lenses

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Application Number Priority Date Filing Date Title
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102928969A (en) * 2011-08-12 2013-02-13 赖博 Microlens-enhanced slide-type confocal optical scanner
CN107101979A (en) * 2016-02-22 2017-08-29 豪夫迈·罗氏有限公司 Optics for analyzing micropore
CN111239047A (en) * 2020-03-09 2020-06-05 深圳中科飞测科技有限公司 Optical equipment and method for realizing automatic focusing

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102928969A (en) * 2011-08-12 2013-02-13 赖博 Microlens-enhanced slide-type confocal optical scanner
CN107101979A (en) * 2016-02-22 2017-08-29 豪夫迈·罗氏有限公司 Optics for analyzing micropore
CN111239047A (en) * 2020-03-09 2020-06-05 深圳中科飞测科技有限公司 Optical equipment and method for realizing automatic focusing
CN111239047B (en) * 2020-03-09 2023-10-27 深圳中科飞测科技股份有限公司 Optical device and method for realizing automatic focusing

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