CN202748305U

CN202748305U - Confocal optical scanner

Info

Publication number: CN202748305U
Application number: CN 201220235234
Authority: CN
Inventors: 赖博
Original assignee: Individual
Current assignee: Individual
Priority date: 2012-05-24
Filing date: 2012-05-24
Publication date: 2013-02-20
Anticipated expiration: 2022-05-24

Abstract

The utility model relates to a confocal optical scanner, in particular to a confocal optical scanner which uses array detectors such as a CCD (Charge Coupled Device), an EMCCD (Electron Multiplying Charge Coupled Devices), a CMOS (Complementary Metal-Oxide-Semiconductor Transistor), and an sCOMS (Service Customizable Overlay Multicast System) as a detecting unit and is applied to biological-fluorescence microscopic imaging. The confocal optical scanner is mainly characterized in that a lighting pinhole array slide block and an imaging pinhole array slide block are arranged in a light path, and pinholes arranged on the two array slide blocks are in one-to-one correspondence; the imaging pinhole array slide block is positioned at the imaging focal plane of a microscope objective, only fluorescence emitted by a sample positioned on the focal plan of the microscope objective is transmitted, and light emitted by a light source is not reflected; and a motor drives the lighting pinhole array slide block and the imaging pinhole array slide block to slide in a reciprocating manner at a set speed so as to realize scanning imaging to the sample. Thus, the confocal optical scanner has the advantages of high scanning speed, zero stray light and background noise, uniform lighting intensity and the like. Meanwhile, the scanning of the confocal optical scanner and the exposure of the detector are easy to realize synchronous control.

Description

A kind of confocal optical scanner

Technical field

The utility model relates to a kind of confocal optical scanner, particularly uses the planar array detectors such as CCD, EMCCD and CMOS as the burnt optical scanner of the copolymerization of detecting device.

Background technology

At present, the existing planar array detectors such as CCD, EMCCD and CMOS that use are as the burnt optical scanner of the copolymerization of detecting device, the main rotation of passing through single rotating disk of a plurality of printing opacity pin holes of arrangement, or arrange a plurality of printing opacity pin holes single sliding block reciprocatingly slide to realize scanning imagery to sample, the light direct irradiation of light source emission is on rotating disk or slide block, there is the light above 96% to be reflected by rotating disk or slide block, causes the high problem of parasitic light noise.By between light source and rotating disk or slide block, placing microlens array, can reduce rotating disk or slide block to the reflection of light source, but the microlens array manufacturing cost too high, be difficult to make and the problems such as size restrictions make it to be difficult to promote.

Summary of the invention

The utility model is for solving the high problem of parasitic light noise, the technical scheme that adopts is: placed illumination pinhole array slide block and imaging pinhole array slide block in the light path, and these two pinhole array have identical pin hole arrangement mode, and all pin holes are one-to-one relationship.The lightproof part of illumination pinhole array slide block, the light reflection that does not see through pin hole with the light source emission makes it not enter imaging optical path, so avoided the high problem of parasitic light noise.

The utility model comprises as shown in Figure 1: light source 1; Exciter filter 2; Collimation lens set 3; Illumination pinhole array slide block 4 has been arranged the pin hole 5 of a plurality of printing opacities on it; Slider bracket 6; Motor 7; Dichroic beam splitter 8; Imaging pinhole array slide block 9 is being arranged a plurality of printing opacity pin holes 10 on it; Imaging lens group 11; Emission color filter 12; Controller 13.

The purpose of this utility model is achieved through the following technical solutions (such as Fig. 1):

Described illumination pinhole array slide block 4 and described imaging pinhole array slide block 9 have been placed, make the described pin hole 5 on the described illumination pinhole array of the light transmission slide block 4 of described light source 1 emission, illumination is positioned at the sample 15 of object lens 14 focal planes of microscope 19, and described illumination pinhole array slide block 4 and described imaging pinhole array slide block 9 have been arranged one to one, the

pin hole

5 and 10 of printing opacity; The fluorescence of described sample 15 emissions sees through the described pin hole 10 of described imaging pinhole array slide block 9, and quilt cover battle array detecting device 16 is recorded as picture; Described illumination pinhole array slide block 4 and described imaging pinhole array slide block 9 are driven by described motor 7, reciprocatingly slide with the speed of setting simultaneously, realize the confocal scanning imaging to described sample 15.

Described

pin hole

5 and 10 be shaped as circle or polygon, such as triangle, square, rectangle, quadrilateral, pentagon and hexagon etc.

Described slider bracket 6 is used for installing, fixing described illumination pinhole array slide block 4 and described imaging pinhole array slide block 9.

Described imaging pinhole array slide block 9 is positioned at the imaging of described object lens 14 of described microscope 19 as the plane, does not reflect the light of described light source 1 emission.

Described exciter filter 2, be positioned at described light 1 source after, its role is to: see through the light of the part wavelength of described light source 1 emission, and stop the light of other wavelength.

Described collimation lens set 3 between described light source 1 and described illumination pinhole array slide block 4, makes the light of described light source 1 emission form parallel beam; The optical axis of described collimation lens set 3 is perpendicular to described illumination pinhole array slide block 4.

Described dichroic beam splitter 8 its role is to the light of described light source 1 emission and the fluorescence of described sample 15 emissions are split up into two light paths.

Described emission color filter 12 is positioned at before described the battle array detecting device 16, its role is to the fluorescence of only launching through described sample 15, the light that reflects other wavelength, makes described battle array detecting device 16 can only detect the fluorescence of described sample 15 emissions.

Described controller 13 has signal and communication with described battle array detecting device 16, control described motor 7 and drive described illumination pinhole array slide block 4 and the simultaneously speed slip to set of described imaging pinhole array slide block 9, make the time shutter of described battle array detecting device 16 equal the integral multiple of the sliding time of described illumination pinhole array slide block 4 and described imaging pinhole array slide block 9, and to make the startup of described motor 7 and the startup of described battle array detecting device 16 exposures be synchronization; And control described light source 1, make 1 of described light source luminous when described battle array detector exposure.

The beneficial effects of the utility model are:

1, sweep velocity is fast, can realize quick co-focusing imaging.

2, avoid the parasitic light of slide block reflection, reduced ground unrest.

Description of drawings

Fig. 1: structural representation of the present utility model

Fig. 2: pinhole array schematic diagram of the present utility model

Fig. 3: the schematic diagram of the first embodiment of the present utility model

Fig. 4: the schematic diagram of the second embodiment of the present utility model

Fig. 5: the schematic diagram of the third embodiment of the present utility model

The drawing explanation:

Light source 1; Exciter filter 2; Collimation lens set 3; Illumination pinhole array slide block 4 has been arranged the pin hole 5 of a plurality of printing opacities on it; Slider bracket 6; Motor 7; Dichroic beam splitter 8; Imaging pinhole array slide block 9 has been arranged the pin hole 10 of a plurality of printing opacities on it; Imaging lens group 11; Emission color filter 12; Controller 13; Micro objective 14; Sample 15; Face battle array detecting device 16; Computing machine 17; Display 18; Microscope 19.

Embodiment

Further describe the present invention below in conjunction with drawings and Examples.

Embodiment 1

Fig. 3 is the first confocal optical scanner schematic diagram relevant with the utility model, and Fig. 2 is the pinhole array schematic diagram of the present embodiment, and pin hole 4a or pin hole 6a are shaped as circle.In addition, pin hole 4a or pin hole 6a also can be polygons, such as triangle, square, rectangle, quadrilateral, pentagon and hexagon etc.

In the present embodiment, when battle array detecting device 16 is unexposed face to face, controller 13 (not shown)s control motor 7 transfixions,

pin hole

5 and 10 does not enter light path, and it is not luminous to control simultaneously light source 1.

Battle array detecting device 16 begins exposure face to face, controller 13 (not shown)s control light source 1 emissive lighting light, and illumination light sees through exciter filter 2 and forms exciting light, becomes parallel exciting light through collimation lens set 3 afterwards; Controller 13 (not shown)s are controlled simultaneously motor 7 and are started, and

pin hole

5 and 10 enters light path, makes the pin hole 5 of parallel exciting light on over-illumination pinhole array slide block 4 be divided into the parallel excitation beam of a plurality of sons; After the parallel excitation beam of son sees through dichroic beam splitter 8, see through the pin hole 10 on the imaging pinhole array slide block 9 fully.Because sub parallel excitation beam sees through the pin hole 10 on the imaging pinhole array slide block 9 fully, so avoided the problem of the lightproof part reflect stray light of imaging pinhole array slide block 9.

The parallel excitation beam of son is through the object lens 14 of microscope 19 (not shown)s, forms one to one lighting point of a plurality of and pin hole 10 at the sample 15 that is positioned at object lens 14 focal planes.The fluorescence of illuminated sample 15 emissions converges through pin hole 10 through object lens 14, is then reflected by dichroic beam splitter 8, sees through imaging lens group 11 and emission color filter 12 and forms and pin hole 10 imaging point one to one at face battle array detecting device 16.

Motor 7 drives illumination pinhole array slide block 4 and imaging pinhole array slide block 9 slides with the speed of setting simultaneously, and lighting point is mobile at sample 15 one to one with pin hole 10, realizes the scanning illumination to sample 15; Imaging point is mobile at face battle array detecting device 16 one to one with pin hole 10 simultaneously.

Face to face during battle array detecting device 16 end exposure, controller 13 (not shown)s control motor is 7 slack, pin hole and 10 shifts out light path, closes simultaneously light source 1, and face battle array detecting device 16 obtains the fluorescence co-focusing image of complete sample 15, and send computing machine 17 to, shown by display 18.

Embodiment 2

Fig. 4 is the structural representation of the second confocal optical scanner related to the present invention, and the difference of embodiment 1 is specific as follows: illumination pinhole array slide block 4 and angle between the imaging pinhole array slide block 9 are 90 to spend; The sub parallel excitation beam emission of dichroic beam splitter 8 reflection sources 1, that form through the pin hole 5 on exciter filter 2, collimation lens set 3 and the illumination pinhole array slide block 4, and see through the fluorescence of sample 16 emissions.

Embodiment 3

Fig. 5 is the structural representation of the third confocal optical scanner relevant with the utility model, and is specific as follows with the difference of embodiment 2: the pin hole 10 of sub parallel excitation beam light source 1 emission, that form through the pin hole 5 on exciter filter 2, collimation lens set 3 and the illumination pinhole array slide block 4 on the imaging pinhole array slide block 9.

Claims

1. confocal optical scanner, illumination pinhole array slide block and imaging pinhole array slide block have been placed, make the described pin hole on the described illumination pinhole array of the light transmission slide block of light source emission, illumination is positioned at the sample of microscopical focal plane of lens, and described illumination pinhole array slide block and described imaging pinhole array slide block have been arranged one to one, the pin hole of printing opacity; The fluorescence of described sample emission sees through the described pin hole of described imaging pinhole array slide block, the imaging of quilt cover battle array detector recording; Described illumination pinhole array slide block and described imaging pinhole array slide block are reciprocatingly slided with the speed of setting simultaneously by motor-driven, realize the confocal scanning imaging to described sample.

2. a kind of confocal optical scanner according to claim 1 is characterized in that, described pin hole be shaped as circle or polygon, such as triangle, square, rectangle, quadrilateral, pentagon and hexagon etc.

3. a kind of confocal optical scanner according to claim 1 also comprises a slider bracket, is used for installing, fixing described illumination pinhole array slide block and described imaging pinhole array slide block.

4. a kind of confocal optical scanner according to claim 1, the imaging that described imaging pinhole array slide block is positioned at described microscopical described object lens do not reflect the light of described light source emission as the plane.

5. a kind of confocal optical scanner according to claim 1 also comprises a slice exciter filter, be positioned at described light source after, its role is to: see through the light of the part wavelength of described light source emission, and stop the light of other wavelength.

6. a kind of confocal optical scanner according to claim 1 also comprises one group of collimation lens set, between described light source and described illumination pinhole array slide block, makes the light of described light source emission form parallel beam; The optical axis of described collimation lens set is perpendicular to described illumination pinhole array slide block.

7. a kind of confocal optical scanner according to claim 1 also comprises a slice dichroic beam splitter, its role is to the light of described light source emission and the fluorescence of described sample emission are split up into two light paths.

8. a kind of confocal optical scanner according to claim 1, also comprise a slice emission color filter, be positioned at before described the battle array detecting device, its role is to the fluorescence of only launching through described sample, the light that reflects other wavelength, make described battle array detecting device can only detect the fluorescence of described sample emission.

9. a kind of confocal optical scanner according to claim 1, comprise that also one has the controller of signal and communication with described battle array detecting device, control described motor-driven described illumination pinhole array slide block and the simultaneously speed slip to set of described imaging pinhole array slide block by described controller, make the time shutter of described battle array detecting device equal the integral multiple of the sliding time of described illumination pinhole array slide block and described imaging pinhole array slide block, and to make the startup of described motor and the startup of described battle array detector exposure be synchronization.

10. a kind of confocal optical scanner according to claim 9, described controller is controlled described light source, makes described light source only luminous when described battle array detector exposure.