CN202232701U - Device for infusing and fixing lungs of experimental animals - Google Patents
Device for infusing and fixing lungs of experimental animals Download PDFInfo
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- CN202232701U CN202232701U CN 201120334412 CN201120334412U CN202232701U CN 202232701 U CN202232701 U CN 202232701U CN 201120334412 CN201120334412 CN 201120334412 CN 201120334412 U CN201120334412 U CN 201120334412U CN 202232701 U CN202232701 U CN 202232701U
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- perfusion
- support bar
- container
- lungs
- animal lungs
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Abstract
The utility model discloses a device for infusing and fixing lungs of experimental animals, comprising a worktable for placing animal lungs, a perfusion container for placing fixing liquid, a perfusion tube and a fixing clip for fixing the perfusion container, wherein the worktable is provided with a support bar; the fixing clip is fixedly connected with the vertical support rod; the perfusion container is fixed on the fixing clip and is vertically located above the worktable; one end of the perfusion tube is connected with the bottom of the perfusion container and is communicated with the perfusion container, and the other end of the perfusion tube is used as a liquid outflow end; and the perfusion tube is provided with a valve for controlling the fixing liquid to flow out. The device for infusing and fixing the lungs of the experimental animals, provided by the utility model, has the advantages of simplicity in structure, convenience for operation and capability of effectively realizing the infusion and fixation of the lungs of the experimental animals.
Description
Technical field
The utility model relates to a kind of laboratory animal lungs perfusion fixture.
Background technology
Zoopery is one of most important the most frequently used scientific research methods in the current scientific research, is multi-disciplinary basic research means such as pharmacology, physiology, Pathological Physiology.Respiratory disease (like COPD, pulmonary vascular disease, lung cancer etc.) is the healthy major disease of harm humans; Therefore the research of respiratory disorder is the important research contents of many medical investigators; The animal model of respiratory disorder has been widely used in the research of respiratory disease pathogenesis and new drug development, and wherein the pathologic data of laboratory animal is the important indicator whether judgment models successfully reaches scientific research.The standardization pathology of laboratory animal lungs is handled the accuracy of pathological examination very important.Lungs have bigger volume compliance, air-breathing when exhaling volume differ bigger, its volume changes because of the weight of animals in addition, desirable fixing means volume of lung when air-breathing is fixed naturally, helps to preserve the natural form of lung tissue like this.But the pathology of lung is fixed not effective perfusion fixture at present.Many employing formalin or 4% paraformaldehyde directly soak fixing, and this fixing means makes the rapid atrophy of lung volume, has oppressed alveolar and vessel lumen, influence the accurate judgement that tip bronchi and vascular pathological change.Also have in addition the researcher with syringe with fixer in tracheae is expelled to the animal lung, but this method can be destroyed the original tissue morphology of animal lung, or because lungs are inhomogeneous full, influence experimental result usually because exert pressure excessively.
Summary of the invention
The purpose of the utility model is to provide a kind of laboratory animal lungs perfusion fixture, and this laboratory animal lungs perfusion fixture simple in structure, easy to operate can realize effectively that the perfusion of laboratory animal lungs fixes.
The purpose of the utility model adopts following technical scheme to realize: a kind of laboratory animal lungs perfusion fixture; Comprise the workbench that is used to place the animal lungs, the perfusion container that is used to hold fixer, perfusion pipe and be used for fixing the geometrical clamp of perfusion container; Described workbench is provided with vertical support bar, and described geometrical clamp is fixedly connected on the support bar, and described perfusion container is fixed on the geometrical clamp; And vertically be positioned at the top of workbench; One end of described perfusion pipe is connected in the bottom of perfusion container, is communicated with the perfusion container, and the other end is as the liquid outflow end; Be used for fixer is imported the animal lungs through tracheae, on described perfusion pipe, be provided with the valve that the control fixer flows out.In when work, the animal lungs are positioned on the workbench, and be positioned at the perfusion container under; In the perfusion container, add fixer; It is poor to form certain height like this between fixer liquid level in the perfusion container and the animal lungs, and the perfusion pipe inserts the interior also ligation of animal lungs tracheae for one section and fixes, and opens valve; Fixer flows into animal lungs, fixedly lung tissue under the effect of gravity.It is fixing fast cmpletely that the utility model both can let the animal lungs obtain, and can guarantee the original natural form of organizing of animal lungs again, and the standardization that is beneficial between the different experiments animal is fixed, and can obtain experimental result accurately.
The size of animal lungs there are differences; In experiment, need to regulate the height of perfusion container; The hydrostatic of fixer generation to satisfy in the infusion containers can evenly be filled into whole lungs with fixer; Do not destroy the structure of lungs blood vessel or tracheae, thereby wherein a kind of embodiment of the utility model is: described geometrical clamp is fixed on the support bar through regulating detent mechanism, can regulate the height of perfusion container according to the experiment needs.
An embodiment as the utility model is: described geometrical clamp comprises stiff end and bare terminal end; Stiff end has shape and the suitable through hole of support bar shape of cross section; Described stiff end slidably is socketed on the support bar through through hole; Radially have the screw of perforation on the sidewall of described through hole; Described adjusting detent mechanism is a screw, this screw one end pass screw and with the sidewall of support bar on inconsistent, the gravity that the frictional force between screw and through-hole wall and support bar sidewall overcomes geometrical clamp and perfusion container is fixed on the support bar geometrical clamp; And described bare terminal end grips the perfusion container, and fixedly the perfusion container is then perpendicular to the horizontal plane of workbench.
The utility model can be done following improvement: described support bar is provided with the longitudinally guiding chute; The end that described screw passes screw is positioned at this guide chute;, this end of screw can move up and down when separating with support bar along chute; Can the quick adjustment geometrical clamp and the height of perfusion container, nor squinted in the position of perfusion container, the operation of convenient experiment.
The utility model can also adopt following embodiment: described support bar is the expansion link of two-part interlocking, regulates the height of perfusion container through the height of regulating expansion link.
The described infusion containers of the utility model is the body that the bottom is provided with liquid outlet, and described liquid outlet is communicated with an end of perfusion pipe.
Be provided with in the side wall upper part of described body and be used for fixer is added liquid inlet in the tube chamber, connect the syringe that quantitative fixer is provided at this liquid inlet place in the perfusion container.In the middle of the use of reality, described syringe can be controlled by automatic control device, at regular time and quantity fixer is added in the perfusion container with realization, makes each perfusion hydraulic pressure identical, has guaranteed the homogeneity of experiment.
The described valve of the utility model adopts unscrewing type valve, plug formula valve or other can realize the purpose valve.
The present invention compared with prior art has following beneficial effect:
(1) the perfusion container of the utility model is positioned at the top of workbench; It is poor to form certain height between the liquid level of the fixer in the perfusion container and the animal lungs on the workbench; Fixer flows in the animal lungs under the effect of self gravitation, and lung tissue is fixed.
(2) fixator of the utility model is to be fixed on slidably on the support bar; Can adjust the height of perfusion container; Make between the animal lungs on fixer liquid level and the workbench difference in height produced hydrostaticly can satisfy fixer and be delivered to whole animal lungs through tracheae, reach the fixedly purpose of lungs tissue, can keep the structural intergrity of tracheae again; Can not cause breaking of tracheae, cause the failure of testing because of pressure excessive.
(3) sidewall of the perfusion container of the utility model is provided with and is used for fixer is added liquid inlet in the tube chamber, connects the syringe that quantitative fixer is provided in the perfusion container at this liquid inlet place.In the middle of the use of reality, described syringe can be controlled by automatic control device, at regular time and quantity fixer is added in the perfusion container with realization, makes each perfusion hydraulic pressure identical, has guaranteed the homogeneity of experiment.
(4) the utility model can be used for lungs fixing that various laboratory animal comprise different experiments animals such as dog, rabbit, rat, mouse; And make simply, easy to operate, cost is low; Normalizable control; Be fit to advantages such as large-scale industrial production, filled up the blank of China laboratory animal lungs standardization body fixator, the experiment utensil of reliable and stable is provided for the fixing standardization of tissues of experimental animals.
Description of drawings
Fig. 1 is the structural representation of the utility model embodiment.
Embodiment
Laboratory animal lungs shown in Figure 1 perfusion fixture is an embodiment of the utility model, and the valve 6 that comprise the workbench 1, the geometrical clamp 3 that is used for fixing the perfusion container that are used to place the animal lungs, be used to hold the perfusion container 4 of fixer, in perfusion container 4, provides syringe 5, perfusion pipe 7 and the control fixer of quantitative fixer to flow out constitutes.Workbench 1 is provided with vertical support bar 2, and the sidewall of support bar 2 is provided with guide chute and scale longitudinally.Geometrical clamp 3 is slidably mounted on the support bar 2 by regulating detent mechanism, and it comprises stiff end 31 and bare terminal end 32, and bare terminal end 32 clamping perfusion containers 4 make it to be vertically mounted on the top of workbench 1.The stiff end 31 of geometrical clamp 3 has the through hole that shape and support bar 2 shape of cross sections match; Radially have the screw of perforation at this through hole corresponding to a side sidewall of the guide chute of support bar 2, this through hole that passes through of geometrical clamp 3 slidably is socketed on the support bar 2, and the adjusting detent mechanism is a screw; This screw one end passes screw and is positioned at guide chute; When tighten the screws makes it with runner wall when inconsistent, the gravity that the frictional force between screw and through-hole wall and support bar 2 sidewalls overcomes geometrical clamp 3 and perfusion container 4 is fixed on the support bar 2 geometrical clamp 3 and perfusion container 4, and when unscrewing screw; Screw separates with support bar 2 with support bar 2 contacted ends with through-hole wall; 3 of geometrical clamps can move up and down along guide chute, can the quick adjustment geometrical clamp 3 with the height of perfusion container 4, and because the existence of guide chute; Can guarantee that when regulating height the position of perfusion container can not squint the operation of convenient experiment.
Infusion containers 4 is provided with the body of liquid outlet for the bottom, is provided for fixer input pipe intracavity liquid input port in the side wall upper part of body, connects the syringe 5 that quantitative fixer is provided in perfusion container 4 at this liquid inlet place.In the middle of the use of reality, described syringe 5 can be controlled by automatic control device, at regular time and quantity fixer is added in the perfusion container 4 with realization, makes each perfusion hydraulic pressure identical, has guaranteed the homogeneity of experiment.The liquid flowing outlet of infusion containers 4 is communicated with an end of perfusion pipe 7, and the other end of perfusion pipe 7 is as the liquid outflow end, is used for fixer through tracheae input animal lungs, and valve 6 is arranged on and flows out with the control fixer on the perfusion pipe 7.Valve 6 can be realized the purpose valve for unscrewing type valve, plug formula valve or other.
When work; The animal lungs are positioned on the workbench; And be positioned at perfusion container 4 under, in perfusion container 4, add the fixer of fixed volume with syringe 5, the formation certain height is poor between the fixer liquid level in the perfusion container 4 and the animal lungs like this; Opening valve 6 fixers can flow in the animal lungs under the effect of gravity, reaches lungs and fixes.The utility model both can obtain fixing fast cmpletely by the animal lungs, can guarantee the original tissue morphology of animal lungs again, and the standardization that is beneficial between the different experiments animal is fixed, and can obtain experimental result accurately.
The embodiment of the utility model is not limited thereto; Foregoing according to the utility model; According to the ordinary skill knowledge and the customary means of this area, do not breaking away under the above-mentioned basic fundamental thought of the utility model prerequisite, each apolegamy of forming combination also can be adopted existing other embodiment of identical function in the utility model; Therefore the utility model can also be made modification, replacement or the change of other various ways, all drops within the utility model rights protection scope.
Claims (8)
1. laboratory animal lungs perfusion fixture is characterized in that, comprises the workbench that is used to place the animal lungs, the perfusion container that is used to hold fixer, perfusion pipe and is used for fixing the geometrical clamp of perfusion container; Described workbench is provided with vertical support bar; Described geometrical clamp is fixedly connected on the support bar, and described perfusion container is fixed on the geometrical clamp, and vertically is positioned at the top of workbench; One end of described perfusion pipe is connected in the bottom of perfusion container; Be communicated with the perfusion container, the other end is provided with the valve that the control fixer flows out as the liquid outflow end on described perfusion pipe.
2. laboratory animal lungs perfusion fixture according to claim 1 is characterized in that described geometrical clamp is fixed on the support bar through regulating detent mechanism.
3. laboratory animal lungs perfusion fixture according to claim 2 is characterized in that described geometrical clamp comprises stiff end and bare terminal end; Stiff end has shape and the suitable through hole of support bar shape of cross section; Described stiff end slidably is socketed on the support bar through through hole, and the sidewall of described through hole radially has the screw of perforation, and described adjusting detent mechanism is a screw; This screw one end pass screw and with the sidewall of support bar on inconsistent; Geometrical clamp is fixed on the support bar, and described bare terminal end grips the perfusion container, and fixedly the perfusion container is then perpendicular to the horizontal plane of workbench.
4. laboratory animal lungs perfusion fixture according to claim 3 is characterized in that described support bar is provided with the longitudinally guiding chute, and an end that passes screw of described screw is positioned at this guide chute as moving up and down slide block along chute.
5. laboratory animal lungs perfusion fixture according to claim 1 is characterized in that described support bar is the expansion link of two-part interlocking, regulates the height of perfusion container through the height of regulating expansion link.
6. according to the described laboratory animal lungs perfusion of the arbitrary claim of claim 1 ~ 5 fixture, it is characterized in that described infusion containers is the body that the bottom is provided with liquid outlet, described liquid outlet is communicated with an end of perfusion pipe.
7. laboratory animal lungs perfusion fixture according to claim 6; It is characterized in that; The side wall upper part of described body is provided with and is used for fixer is added liquid inlet in the tube chamber, and this liquid inlet place connects the syringe that quantitative fixer is provided in the perfusion container.
8. laboratory animal lungs perfusion fixture according to claim 7 is characterized in that described valve adopts unscrewing type valve or plug formula valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120334412 CN202232701U (en) | 2011-09-07 | 2011-09-07 | Device for infusing and fixing lungs of experimental animals |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120334412 CN202232701U (en) | 2011-09-07 | 2011-09-07 | Device for infusing and fixing lungs of experimental animals |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202232701U true CN202232701U (en) | 2012-05-30 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120334412 Expired - Fee Related CN202232701U (en) | 2011-09-07 | 2011-09-07 | Device for infusing and fixing lungs of experimental animals |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202232701U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102787095A (en) * | 2012-07-06 | 2012-11-21 | 广州医学院第一附属医院 | Method for cultivating rat primary airway epithelial cells |
| CN105325403A (en) * | 2015-12-09 | 2016-02-17 | 广东省中医院 | Rapid hanging and fixing device for isolated perfused heart |
-
2011
- 2011-09-07 CN CN 201120334412 patent/CN202232701U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102787095A (en) * | 2012-07-06 | 2012-11-21 | 广州医学院第一附属医院 | Method for cultivating rat primary airway epithelial cells |
| CN105325403A (en) * | 2015-12-09 | 2016-02-17 | 广东省中医院 | Rapid hanging and fixing device for isolated perfused heart |
| CN105325403B (en) * | 2015-12-09 | 2017-10-31 | 广东省中医院 | Isolated perfused heart quick suspension and fixing device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120530 Termination date: 20140907 |
|
| EXPY | Termination of patent right or utility model |