CN202083699U - Kit used for predicating premature delivery pregnant woman - Google Patents
Kit used for predicating premature delivery pregnant woman Download PDFInfo
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- CN202083699U CN202083699U CN2011201671560U CN201120167156U CN202083699U CN 202083699 U CN202083699 U CN 202083699U CN 2011201671560 U CN2011201671560 U CN 2011201671560U CN 201120167156 U CN201120167156 U CN 201120167156U CN 202083699 U CN202083699 U CN 202083699U
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- pad
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- nitrocellulose membrane
- monoclonal antibody
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Abstract
The utility model discloses a kit used for predicating premature delivery of a pregnant woman, which comprises a card box formed by overlapping a kit cover with a kit body; moreover, the kit used for predicating premature delivery of pregnant woman also comprises a reagent strip arranged in the kit body through an extruding piece playing the role of fixation; the bottom layer of the reagent strip is a plastic pad; one end of the plastic pad is stuck with a section of sample pad; a section of goldlabelled pad partially embedded into the sample pad, stuck on the plastic pad and coated with a mouse anti-human fFN monoclonal antibody is close to the sample pad; a section of nitrocellulose membrane partially embedded in the goldlabelled pad and stuck on the plastic pad is close to the goldlabelled pad; a T line of a detection belt containing the mouse anti-human fFN monoclonal antibody and a C line of a quality control belt containing goat anti-mouse IgG are coated on the nitrocellulose membrane; and the nitrocellulose membrane is also partially mebedded into an absorbent pad close to the nitrocellulose membrane and stuck on the plastic pad. A pair of monoclonal antibodies is used for forming a detection kit. Compared with current like products employing monoclonal antibody and polyclonal antibody combination, the production cost can be reduced and the production efficiency can be improved.
Description
Technical field:
The utility model relates to a kind of kit, particularly a kind of kit that is used for the premature labor in pregnant women prediction that adopts the double antibody sandwich method of a pair of monoclonal antibody formation.
Background technology:
As everyone knows, (Fetal Fibronectin fFN) is a kind of glycoprotein to fetal fibronectin, rises by the decidua secretion and to chorion and decidua to be connected and adhesive attraction.It can be used in the prediction of 22-35 week pregnant woman's threatened premature labor.There are premature labor symptom pregnant woman to check in asymptomatic pregnant woman's of pregnant week of 22-35 conventional examination and pregnant week of 24-35.
At present, only there is the product of U.S. ADEZA company to be used for clinical detection in the world.The product of ADEZA company adopts monoclonal antibody and collaurum solid-phase immunity chromatography, the end product of many anti-combinations to come fetal fibronectin content in the qualitative determination uterine neck vaginal secretion with the form of test strips.That is: with collaurum as the cue mark thing crosslinked anti--the fFN monoclonal antibody, bag is by on sample pad, simultaneously on nitrocellulose membrane with the anti-fFN polyclonal antibody of immobilization rabbit as detection line, and bag by sheep anti-mouse igg as nature controlling line.
But, above-mentioned this test strips adopts monoclonal antibody and many anti-sandwich methods that make up, the how anti-detection line that is used for wherein.Because polyclonal antibody is a mixture, by all can discern the B emiocytosis of this antigen in the animal body.Can discern a plurality of different epitopes on this antigen.Polyclonal antibody heterogeneity extremely in view of chemical constitution is highly heterogeneous.When preparing antibody, all need highly purified antigen-immunized animal, to obtain corresponding antibodies at every turn.Simultaneously, the antibody that obtains not only needs complicated processing obtaining to meet the antibody of quality, and quality is also non-constant, and difference between batch is very big.In addition, adopt during production polyclonal antibody not only to waste time and energy but also can not guarantee the constancy of quality fully.Make that production efficiency is low, and cost is also very high in the preparation.
The utility model content:
The utility model provides a kind of kit that is used for the premature labor in pregnant women prediction.This kit is formed detection kit with a pair of monoclonal antibody, the existing like product that adopts the sandwich method of monoclonal antibody and many anti-combinations of comparing, and kit described in the utility model can reduce production costs, enhance productivity.
Concrete technical scheme of the present utility model is as follows:
A kind of kit that is used for premature labor in pregnant women prediction is characterized in that, comprises a cartridge that is overlapped into by lid and box body, and one is placed in reagent strip in the box body by fixing with protrusion; The bottom of described reagent strip is a plastic gasket, and an end of described plastic gasket is pasted with one section sample pad, and next-door neighbour's sample is lined with one section gold mark that is coated with mouse-anti people fFN monoclonal antibody that is attached on the plastic gasket that is partially submerged into sample pad and fills up; Next-door neighbour gold mark is lined with one section nitrocellulose membrane on the plastic gasket of being attached to that is partially submerged into gold mark pad; Be coated with another detection band T line that contains mouse-anti people fFN monoclonal antibody and the quality control band C line that contains sheep anti-mouse igg on the described nitrocellulose membrane; Described nitrocellulose membrane also be partially submerged into the next-door neighbour it be attached to adsorptive pads on the plastic gasket; The position of the interior reagent strip sample pad of corresponding box body has a well that is used for adding sample on the described lid; The detection band T line and the quality control band C line position of the interior reagent strip of corresponding box body have a display window that is used for showing test results on the described lid.
It is pointed out that the sample liquid that above-mentioned this kit is mainly used to detect is cervical secretions.
Kit described in the utility model adopts a pair of monoclonal antibody to form double antibody sandwich method.Because the advantage of monoclonal antibody is: monoclonal antibody is secreted by same strain B cell clone, the gene order of this antibody of encoding is in full accord, only discerns single epitope, its structural similarity and homogeneous, high degree of specificity is arranged, and it is anti-better than Duoing to say monoclonal antibody on the specificity.Though Monoclonal Antibody technical sophistication, time-consuming taking a lot of work during initial preparation, but but in case after obtaining the hybridoma of secrete monoclonal antibody, hybridoma can be survived " forever " and goes down to posterity external, only otherwise the gene mutation of cell line takes place, just can constantly produce the antibody of high specific, high homogeneity.When kit is produced, can utilize the characteristic of monoclonal antibody to obtain the antibody of a large amount of special and constant mass like this, avoid polyclonal antibody to waste time and energy and can not guarantee the constant shortcoming of quality fully.Thereby in a disguised form reduced production cost, enhanced productivity.
In addition, kit of the present utility model only needs to drip sample liquid in use in well and gets final product, need not as the available reagent bar, the whole piece reagent strip be put into the test tube that fills sample liquid and be detected, the utility model is simple more, convenient on the use-pattern like this.
Description of drawings:
Further specify the utility model below in conjunction with the drawings and specific embodiments.
Fig. 1 is the front appearance figure of kit described in the utility model.
Fig. 2 is the structural drawing of the cartridge lid of kit described in the utility model.
Fig. 3 is the structural drawing of the cartridge box body inside of kit described in the utility model.
Fig. 4 is the structural drawing of the reagent strip of kit described in the utility model.
Embodiment:
For technological means, creation characteristic that the utility model is realized, reach purpose and effect is easy to understand, below in conjunction with concrete diagram, further set forth the utility model.
Referring to Fig. 1-Fig. 4, the kit that is used for premature labor in pregnant women prediction described in the utility model comprises a cartridge 100 that is overlapped into by lid 110 and box body 120, and one is placed in the interior reagent strip 200 of box body 120 by fixing with protrusion 300.
Referring to Fig. 2 and Fig. 3, the position of reagent strip sample pad has a well 111 that is used for adding sample in the corresponding box body 120 on lid 110; The detection band T line 204 and quality control band C line 205 positions of corresponding box body 120 interior reagent strips have a display window that is used for showing test results 112 on the described lid 110.
As shown in Figure 4, the bottom of reagent strip 200 is a plastic gasket 207, one end of described plastic gasket 207 is pasted with one section sample pad 202, and next-door neighbour's sample pad 202 has one section gold mark that is coated with mouse-anti people fFN monoclonal antibody that is attached on the plastic gasket 207 that is partially submerged into sample pad 202 to fill up 206; Next-door neighbour gold mark pad 206 has one section nitrocellulose membrane 201 on the plastic gasket 207 of being attached to that is partially submerged into gold mark pad 206; Be coated with another detection band T line that contains mouse-anti people fFN monoclonal antibody 204 and the quality control band C line 205 that contains sheep anti-mouse igg on the described nitrocellulose membrane 201; Described nitrocellulose membrane 201 also be partially submerged into the next-door neighbour it be attached to adsorptive pads 203 on the plastic gasket 207;
The mentioned reagent box in use, as long as sample liquid (cervical secretions) is splashed in the well 111 of kit, like this after the fFN in the sample liquid enters the sample application zone of test strips, the mouse-anti people fFN monoclonal antibody of gold mark pad 206 (colloid gold labels) combines with the fFN antigen in the sample, formed Ag-Ab colloidal gold composite moves at capillary effect lower edge nitrocellulose membrane 201, combines with another mouse-anti of immobilization people fFN monoclonal antibody at detection band T line 204 places and forms a red detection line; The monoclonal antibody of unnecessary colloid gold label then continues to move to quality control band C line 205 places, forms another red nature controlling line with sheep anti-mouse igg.As no fFN antigen in the sample, then can not form red detection line, only can be observed a red nature controlling line.
More than show and described ultimate principle of the present utility model and principal character and advantage of the present utility model.The technician of the industry should understand; the utility model is not restricted to the described embodiments; that describes in the foregoing description and the instructions just illustrates principle of the present utility model; under the prerequisite that does not break away from the utility model spirit and scope; the utility model also has various changes and modifications, and these changes and improvements all fall in claimed the utility model scope.The claimed scope of the utility model is defined by appending claims and equivalent thereof.
Claims (1)
1. a kit that is used for premature labor in pregnant women prediction is characterized in that, comprises a cartridge that is overlapped into by lid and box body, and one is placed in reagent strip in the box body by fixing with protrusion; The bottom of described reagent strip is a plastic gasket, and an end of described plastic gasket is pasted with one section sample pad, and next-door neighbour's sample is lined with one section gold mark that is coated with mouse-anti people fFN monoclonal antibody that is attached on the plastic gasket that is partially submerged into sample pad and fills up; Next-door neighbour gold mark is lined with one section nitrocellulose membrane on the plastic gasket of being attached to that is partially submerged into gold mark pad; Be coated with another detection band T line that contains mouse-anti people fFN monoclonal antibody and the quality control band C line that contains sheep anti-mouse igg on the described nitrocellulose membrane; Described nitrocellulose membrane also be partially submerged into the next-door neighbour it be attached to adsorptive pads on the plastic gasket; The position of the interior reagent strip sample pad of corresponding box body has a well that is used for adding sample on the described lid; The detection band T line and the quality control band C line position of the interior reagent strip of corresponding box body have a display window that is used for showing test results on the described lid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011201671560U CN202083699U (en) | 2011-05-13 | 2011-05-13 | Kit used for predicating premature delivery pregnant woman |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011201671560U CN202083699U (en) | 2011-05-13 | 2011-05-13 | Kit used for predicating premature delivery pregnant woman |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202083699U true CN202083699U (en) | 2011-12-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011201671560U Expired - Fee Related CN202083699U (en) | 2011-05-13 | 2011-05-13 | Kit used for predicating premature delivery pregnant woman |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202083699U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108700587A (en) * | 2016-02-24 | 2018-10-23 | 积水医疗株式会社 | The method for detecting cancer embryo fibronectin using immunochromatography |
-
2011
- 2011-05-13 CN CN2011201671560U patent/CN202083699U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108700587A (en) * | 2016-02-24 | 2018-10-23 | 积水医疗株式会社 | The method for detecting cancer embryo fibronectin using immunochromatography |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20111221 Termination date: 20200513 |