CN202066790U - Electrochemical luminescence imaging device - Google Patents
Electrochemical luminescence imaging device Download PDFInfo
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- CN202066790U CN202066790U CN2011201737873U CN201120173787U CN202066790U CN 202066790 U CN202066790 U CN 202066790U CN 2011201737873 U CN2011201737873 U CN 2011201737873U CN 201120173787 U CN201120173787 U CN 201120173787U CN 202066790 U CN202066790 U CN 202066790U
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- electrolytic cell
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- electron multiplication
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Abstract
Provided is an electrochemical luminescence imaging device. A platform is arranged at the bottom inside a hidden box and provided with an electrolytic cell and a support. A lifting sleeve is arranged on the support and provided with a fine-adjustment bolt and a fixing board. The fixing board is provided with an electron multiplying charge-coupled device, a lower end surface of the electron multiplying charge-coupled device is provided with at least one extension ring, and the lower end of the extension ring is provided with a standard lens. An electrochemical work station and a computer are arranged outside the hidden box. The electrochemical work station is connected with the electrolytic cell through a wire and the computer is connected with the electrochemical work station and the electron multiplying charge-coupled device respectively through a data line. The electrochemical luminescence imaging device has the advantages of being reasonable in design, simple in structure, high in imaging sensitivity and the like and can serve as an electrochemical luminescence imaging experimental apparatus.
Description
Technical field
The utility model belongs to electrochemiluminescence checkout equipment technical field, is specifically related to utilize the electron multiplication charge-coupled image sensor to carry out the instrument of electrochemiluminescence imaging.
Background technology
In life science, clinical diagnosis detection and pathological study, because the complicacy of clinical diagnosis and pathological study, more and more pay attention to " how information-based " and the technology of " visual " and the development of pertinent instruments, more and more pay attention to biochip technology and various medical imaging diagnosis technology.For example, the early diagnosis of cancer and making a definite diagnosis promptly needs the biochemical analysis method of high sensitivity fast detecting kinds of tumors marker, the image analysing computer of the biopsy that also needs to cut into slices.And the foundation of these methods development depends on the development of related science instrument.In recent years, various optical imagery analytical instrument, as fluorescence imaging instrument, chemiluminescence imager with its high sensitivity resolution and visual imaging, forward position and focus for the chemical analysis technology area research, but there are the shortcomings such as photobleaching, the spectra overlapping between the dye molecule and matrix interference of fluorescence probe in the fluorescent microscopic imaging technology, and chemical luminous chip is subjected to the restriction that application of sample control reaction starts, though highly sensitive, but restive, its application is subjected to certain restriction.
Electrochemiluminescence is to apply certain voltage to make between the electrode reaction product or carry out chemical reaction between certain component in electrode reaction product and the solution and a kind of optical radiation of producing on electrode.The two-fold advantage of electrochemiluminescence method combining electrochemical and chemoluminescence method, advantage such as the range of linearity, the instrument and equipment with highly sensitive, lower detectability, broad is simple, easy to operate, easily be automated; With respect to fluorescence method, no photobleaching does not need light source and beam splitting system; With respect to chemiluminescence, has the strong advantage of controllability; With respect to Electrochemical Detection, it is low and be subjected to electrode fouling to influence little advantage to have a detectability.Therefore, development electrochemiluminescence Image-forming instrument is a research topic with important scientific meaning and actual application value.
Summary of the invention
Technical problem to be solved in the utility model is to overcome the shortcoming of above-mentioned fluorescence imaging instrument, chemiluminescence imager, and a kind of reasonable in design, simple in structure, electrochemiluminescence imaging device that imaging is highly sensitive is provided.
Solving the problems of the technologies described above the technical scheme that is adopted is: the bottom in camera bellows is provided with platform, platform is provided with electrolytic cell and support, support is provided with the lifting cover, the lifting cover is provided with trimming bolt and fixed head, fixed head is provided with the electron multiplication charge-coupled image sensor, electron multiplication charge-coupled image sensor lower surface is provided with 1 extension ring at least, the lower end of extension ring is provided with standard lens, camera bellows is outside equipped with electrochemical workstation and computing machine, electrochemical workstation links to each other with electrolytic cell by lead, and computing machine links to each other with electrochemical workstation by data line, link to each other with the electron multiplication charge-coupled device by data line.
Electrolytic cell of the present utility model is: contrast electrode that the end is connected with electrode contact, working electrode are set, to electrode on substrate, also are provided with the electrolytic cell groove on the substrate.
The utility model adopts electrochemical workstation to apply different or identical voltage to electrode, in the electrolytic cell or the chemical illuminating reagent generation chemiluminescence reaction of electrode surface, produce light signal, through optical lens, gathered by the inductive coupling device, and convert the picture signal of electrode surface to electric signal input computing machine and carry out data read and Flame Image Process, obtain the details of electrode microcell electrochemiluminescence, expose adjustment, automatic focusing, post-processed processing is adjusted, and obtains the high image of sharpness.The utlity model has advantages such as reasonable in design, simple in structure, imaging sensitivity height, can be used as electrochemiluminescence imaging experiment instrument.
Description of drawings
Fig. 1 is the structural representation of an embodiment of the utility model.
Fig. 2 is the structural representation of electrolytic cell 8 among Fig. 1.
Embodiment
Below in conjunction with drawings and Examples the utility model is further described, but the utility model is not limited to these embodiment.
Embodiment 1
In Fig. 1, the electric electrochemiluminescence imaging device of present embodiment is made of camera bellows 1, support 2, fixed head 3, electron multiplication charge-coupled device 4, computing machine 5, electrochemical workstation 6, platform 7, electrolytic cell 8, standard lens 9, extension ring 10, trimming bolt 11, lifting cover set bolt 12,13 connections of lifting cover.
Bottom in camera bellows 1 is with the screw threads for fastening connector platform 7 that has been fixedly connected, on the platform 7 with the screw threads for fastening connector electrolytic cell 8 that has been fixedly connected, by thread connection support 2 is installed on the platform 7, be processed with tooth bar on the support 2, be installed with lifting cover 13 with lifting cover set bolt 12 on the support 2, in the lifting cover 13 gear is installed, the wheel and rack engagement constitutes pinion and rack conventional in the Machine Design, on the lifting cover 13 trimming bolt 11 is installed, trimming bolt 11 is fixedly connected with gear, rotary fine adjustment bolt 11, the lifting cover can move up and down along support 2.On the lifting cover 13 with the screw threads for fastening connector fixed head 3 that has been fixedly connected, on the fixed head 3 with the screw threads for fastening connector electron multiplication charge-coupled image sensor 4 that has been fixedly connected, the commodity of electron multiplication charge-coupled image sensor 4 for selling on the market, model is ixon+897, sell by company of Britain Andor company, the center connection of electron multiplication charge-coupled image sensor 4 lower surfaces is equipped with 1 extension ring 10, the quantity of extension ring 10 can increase and decrease as required, the lower end of extension ring 10 is equipped with standard lens 9, extension ring 10 has shortened the object distance of standard lens 9, reduced light loss, the distance between electron multiplication charge-coupled device 4 and the electrolytic cell 8 is regulated by adjusting trimming bolt 11.Camera bellows 1 is outside equipped with electrochemical workstation 6 and computing machine 5, the commodity of electrochemical workstation 6 for selling on the market, model is CHI660, sell by Shanghai occasion China instrument company, electrochemical workstation 6 links to each other with electrolytic cell 8 by lead, and computing machine 5 links to each other with electrochemical workstation 6, links to each other with electron multiplication charge-coupled device 4 by lead by data line.
In Fig. 2, the electrolytic cell 8 of present embodiment connects by electrode contact 8-1, electrolytic cell groove 8-2, contrast electrode 8-3, working electrode 8-4, to electrode 8-5, substrate 8-6 and constitutes.On substrate 8-6, be coated be brushed with carbon slurry be made into contrast electrode 8-3, working electrode 8-4, to electrode 8-5, contrast electrode 8-3, working electrode 8-4, to the end of the electrode 8-5 electrode contact 8-1 that all has been connected as a single entity.Stick with glue on the substrate 8-6 and be connected to electrolytic cell groove 8-2, electrolytic cell groove 8-2 is used to adorn chemical reagent and the chemical illuminating reagent that detects usefulness.
In the present embodiment, in the connection of the center of electron multiplication charge-coupled image sensor 4 lower surfaces 4 extension rings 10 are installed, the lower end of extension ring 10 is equipped with standard lens 9.Other parts and; The annexation of parts is identical with embodiment 1.
The course of work of the present utility model is as follows:
During experiment, open camera bellows 1 earlier, in electrolytic cell 8, add electrochemiluminescence reagent, as ruthenium dipyridine-tripropyl amine (TPA), luminol-hydrogen peroxide, by the distance between rotation and lifting cover set bolt 12 and trimming bolt 11 adjustment electron multiplication charge-coupled devices 4 and the electrolytic cell 8, make the electrode in the electrolytic cell 8 on computing machine 5, present distinct image, close camera bellows 1, according to the program of prior setting electrochemical workstation 6 is sent signal to the electrode application voltage signal in the electrolytic cell 8 by computing machine 5, electrochemiluminescence reagent in the electrolytic cell 8 produces the electrochemiluminescence reaction, electron multiplication charge-coupled device 4 receives light signal that electrochemiluminescence reagent produces the electrochemiluminescence reaction and converts electric signal to and output to computing machine 5, presents electrochemiluminescence image clearly on computing machine 5.If the light signal of electrochemiluminescence reaction is very weak,, also can on computing machine 5, present electrochemiluminescence image clearly by adjusting the time shutter and the gain of electron multiplication charge-coupled device 4.
Claims (2)
1. electrochemiluminescence imaging device, it is characterized in that: the bottom in camera bellows (1) is provided with platform (7), platform (7) is provided with electrolytic cell (8) and support (2), support (2) is provided with lifting cover (13), lifting cover (13) is provided with trimming bolt (11) and fixed head (3), fixed head (3) is provided with electron multiplication charge-coupled image sensor (4), electron multiplication charge-coupled image sensor (4) lower surface is provided with 1 extension ring (10) at least, the lower end of extension ring (10) is provided with standard lens (9), camera bellows (1) is outside equipped with electrochemical workstation (6) and computing machine (5), electrochemical workstation (6) links to each other with electrolytic cell (8) by lead, and computing machine (5) links to each other with electrochemical workstation (6) by data line, link to each other with electron multiplication charge-coupled device (4) by data line.
2. according to the described electrochemiluminescence imaging device of claim 1, it is characterized in that described electrolytic cell (8) is: contrast electrode (8-3) that the end is connected with electrode contact (8-1), working electrode (8-4) are set, to electrode (8-5) on substrate (8-6), also are provided with electrolytic cell groove (8-2) on the substrate (8-6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011201737873U CN202066790U (en) | 2011-05-26 | 2011-05-26 | Electrochemical luminescence imaging device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011201737873U CN202066790U (en) | 2011-05-26 | 2011-05-26 | Electrochemical luminescence imaging device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202066790U true CN202066790U (en) | 2011-12-07 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011201737873U Expired - Fee Related CN202066790U (en) | 2011-05-26 | 2011-05-26 | Electrochemical luminescence imaging device |
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| CN (1) | CN202066790U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102621080A (en) * | 2012-04-10 | 2012-08-01 | 中国科学技术大学 | Field electrochemical microspectroscopic imaging analysis method and system |
| CN102689853A (en) * | 2012-05-31 | 2012-09-26 | 福州展旭电子有限公司 | Externally controllable built-in lift platform |
| CN102755166A (en) * | 2012-06-20 | 2012-10-31 | 浙江大学 | Method for directly showing latent fingerprints based on multiple electrochemistry lighting systems |
-
2011
- 2011-05-26 CN CN2011201737873U patent/CN202066790U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102621080A (en) * | 2012-04-10 | 2012-08-01 | 中国科学技术大学 | Field electrochemical microspectroscopic imaging analysis method and system |
| CN102621080B (en) * | 2012-04-10 | 2014-03-19 | 中国科学技术大学 | Field electrochemical microspectroscopic imaging analysis method and system |
| CN102689853A (en) * | 2012-05-31 | 2012-09-26 | 福州展旭电子有限公司 | Externally controllable built-in lift platform |
| CN102689853B (en) * | 2012-05-31 | 2015-05-20 | 福州展旭电子有限公司 | Externally controllable built-in lift platform |
| CN102755166A (en) * | 2012-06-20 | 2012-10-31 | 浙江大学 | Method for directly showing latent fingerprints based on multiple electrochemistry lighting systems |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20111207 Termination date: 20140526 |