CN201974574U - Inverted digital holographic microscope - Google Patents
Inverted digital holographic microscope Download PDFInfo
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- CN201974574U CN201974574U CN2011200612122U CN201120061212U CN201974574U CN 201974574 U CN201974574 U CN 201974574U CN 2011200612122 U CN2011200612122 U CN 2011200612122U CN 201120061212 U CN201120061212 U CN 201120061212U CN 201974574 U CN201974574 U CN 201974574U
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- optical fiber
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- fiber collimator
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- collimator
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- 239000013307 optical fiber Substances 0.000 claims abstract description 40
- 239000000835 fiber Substances 0.000 claims abstract description 28
- 230000003287 optical effect Effects 0.000 claims abstract description 9
- 238000013519 translation Methods 0.000 claims abstract description 6
- 238000003384 imaging method Methods 0.000 abstract description 6
- 238000001093 holography Methods 0.000 abstract description 4
- 238000005259 measurement Methods 0.000 abstract description 3
- 239000008710 crystal-8 Substances 0.000 abstract 2
- 239000000523 sample Substances 0.000 description 18
- 210000004027 cell Anatomy 0.000 description 9
- 239000012472 biological sample Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 210000001082 somatic cell Anatomy 0.000 description 2
- 101150030337 CCD7 gene Proteins 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000009510 drug design Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000008611 intercellular interaction Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000010587 phase diagram Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 238000012876 topography Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/04—Processes or apparatus for producing holograms
- G03H1/0443—Digital holography, i.e. recording holograms with digital recording means
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/0005—Adaptation of holography to specific applications
- G03H2001/005—Adaptation of holography to specific applications in microscopy, e.g. digital holographic microscope [DHM]
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/04—Processes or apparatus for producing holograms
- G03H1/0443—Digital holography, i.e. recording holograms with digital recording means
- G03H2001/0454—Arrangement for recovering hologram complex amplitude
- G03H2001/0456—Spatial heterodyne, i.e. filtering a Fourier transform of the off-axis record
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H2210/00—Object characteristics
- G03H2210/62—Moving object
-
- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H2223/00—Optical components
- G03H2223/16—Optical waveguide, e.g. optical fibre, rod
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- Engineering & Computer Science (AREA)
- Computing Systems (AREA)
- Theoretical Computer Science (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Holo Graphy (AREA)
- Microscoopes, Condenser (AREA)
Abstract
The utility model discloses an inverted digital holographic microscope which belongs to the technical field of digital holography and can be applied to three-dimensional real-time shape measurement and biological cell imaging. A fiber coupler 4 is placed in front of a laser 5 and connected with an optical fiber splitter 3 through optical fibers; the optical fiber splitter 3 is provided with two paths of optical fibers which are connected with a fiber collimator 1 and a fiber collimator 6 respectively; a sample stage 11 used for bearing samples 13 is placed below the fiber collimator 1 and connected to a two-dimensional translation stage 12; a microobjective 9 installed on a one-dimensional translation stage 10 is placed below the sample stage 11; the microobjective 9 and the fiber collimator 6 are aligned to two side surfaces of a combining crystal 8 which are vertical to each other; and a CCD camera 7 is placed below the combining crystal 8. The inverted digital holographic microscope can conduct long-time and high-resolution observation on living cells growing and adhering to the wall of the bottom of a petri dish, the microscope is highly integrated and small in volume, and due to the adoption of optical link, the laser can be installed at other parts of a system randomly.
Description
Technical field
The utility model discloses a kind of inversion type digital hologram microscope, belong to the digital holography techniques field, can be used for three-dimensional topography measurement in real time, the biological cell imaging.
Background technology
Biomedical Development has promoted the development of observation technology on the biological cell yardstick in recent years.Traditional optical microscope, the not three-dimensional appearance of energy measurement biological cell; Though and confocal microscope resolution is higher, owing to will do demarcation, can exert an influence to it to biological sample, be unfavorable for the needs of harmless observation.Digital hologram is as a kind of micro-imaging technique, and its characteristics harmless, real-time, that can obtain the quantitative phase distribution are its advantage in the biological sample imaging just.The living body biological cell is generally transparent configuration, so its phase image can provide the information of more uniquenesses.Be different from existing phase contrast imaging method, Digital Holography do not need to the living body biological sample carry out mark, processings such as fixing just can obtain quantitative amplitude of the object of observation and PHASE DISTRIBUTION, thereby realization is to the imaging of transparent organism sample and carry out quantitative test.Digital Holography can also realize the dynamic monitoring to the biological sample form, then may be used to obtain cell dynamic perfromance, intercellular interaction and the cell information such as reaction to medicine, expecting provides certain assay foundation for early stage medical diagnosis and drug design etc.The Lyncee Tec company of Switzerland is unique digital hologram microscope manufacturer, but its digital hologram microscope adopts upright structure, because the limited operating distance of microcobjective, particularly the very short operating distance of high magnification microcobjective can't be done real-time high resolution observations to the sample of growth bottom double dish or nutrient solution; Because existing microscopic system is to be made of space optical path, integral body weighs greatly, introduces fiber coupling system and can effectively reduce microscopical volume and weight, and can reduce the influence of the slight vibrations of laser instrument to the generation of measuring system precision
The utility model content
Big in order to solve digital hologram microscope volume and weight, and take upright structure can't observe the technical matters of active somatic cell in the double dish, the utility model proposes a kind of inversion type digital hologram microscope.
The utility model adopts following technical scheme: the place ahead of laser instrument 5 is mounted with fiber coupler 4, fiber coupler 4 links to each other with fiber optic splitter 3 by optical fiber, fiber optic splitter 3 picks out two-way optical fiber and is connected with optical fiber collimator 1 and optical fiber collimator 6 respectively, optical fiber collimator 1 below is equipped with the sample stage 11 that is used to hold sample 13, sample stage 11 is connected on the 12. two-dimension translational platforms, sample stage 11 belows are equipped with the microcobjective 9 that is installed on the one dimension translation stage 10, and microcobjective 9 is aimed at two perpendicular sides of closing Shu Jingti 8 with optical fiber collimator 6.Close Shu Jingti 8 belows and be equipped with CCD camera 7.CCD7 links to each other with computing machine 14.
The light of laser instrument 5 outgoing is through fiber coupler 4 coupled into optical fibres, and be divided into two-way by fiber optic splitter 3: the first via is a thing light, by an optical fiber collimator 1 spherical light wave of dispersing of optical fiber outgoing is collimated into directional light, directional light is radiated on the sample 13 of horizontal positioned straight down, pass sample stage 11, microcobjective 9, and by being radiated at behind the beam cementing prism 8 on the CCD camera 7; Another road is a reference light, is shaped to directional light by optical fiber collimator 6, and level is radiated on the beam cementing prism 8, and the reflection back forms hologram by CCD camera 7. records with the thing optical interference; Above-mentioned two-way light has angle.
Above-mentioned thing light is 5mm through the diameter of the directional light that optical fiber collimator 1 is collimated into.
Above-mentioned reference light is 2cm through the directional light diameter that optical fiber collimator 6 is collimated into.
The utility model can be obtained following beneficial effect:
This cover utility model can be done long-time high resolution observations to the active somatic cell of double dish bottom adherent growth, is not placed in the handtailor container and observes and do not need that living cells is broken away from its culture environment.
The level of integrated system height, volume is little, and optics lacks than common system, and makes laser instrument can be installed in other positions of system arbitrarily owing to adopt optical fiber to connect, and has avoided the influence of the slight vibrations of laser instrument to system accuracy.
Description of drawings
The microscopical structure principle chart of Fig. 1 inversion type digital hologram;
The hologram that Fig. 2 CCD camera is adopted
The partial enlarged drawing of Fig. 3 hologram
The 3-D display figure that in computer, is generated behind the sample process inversion type digital hologram microscope among Fig. 4 embodiment;
The gray scale displayed map that in computer, is generated behind the sample process inversion type digital hologram microscope among Fig. 5 embodiment;
Among the figure: 1,6, optical fiber collimator, 2, optical fiber, 3, fiber optic splitter, 4, fiber coupler, 5, laser instrument, 7, CCD camera, 8, close Shu Jingti, 9 microcobjectives, 10, the one dimension translation stage, 11 sample stage, 12 two-dimension translational platforms, 13, sample, 14, computing machine.
Embodiment
Be described further for the utility model below in conjunction with the drawings and specific embodiments:
The arrangement of present embodiment is as shown in Figure 1:
The place ahead of laser instrument 5 is mounted with fiber coupler 4, fiber coupler 4 links to each other with fiber optic splitter 3 by optical fiber, fiber optic splitter 3 picks out two-way optical fiber and is connected with optical fiber collimator 1 and optical fiber collimator 6 respectively, optical fiber collimator 1 below is equipped with the sample stage 10 that is used to hold sample 11, sample stage 10 belows are equipped with microcobjective 9, and microcobjective 9 is aimed at two perpendicular sides of closing Shu Jingti 8 with optical fiber collimator 6.Close Shu Jingti 8 belows and be equipped with CCD camera 7.
The light of laser instrument 5 outgoing is through fiber coupler 4 coupled into optical fibres, and be divided into two-way by fiber optic splitter 3: the first via is a thing light, by an optical fiber collimator 1, the spherical light wave of dispersing of optical fiber outgoing is collimated into directional light, directional light is radiated on the sample 11 of horizontal positioned straight down, pass sample stage 10, microcobjective 9, and by being radiated at behind the beam cementing prism 8 on the CCD camera 7; Another road is a reference light, is shaped to directional light by optical fiber collimator 6, and level is radiated on the beam cementing prism 8, and the reflection back forms hologram by CCD camera 7 records with the thing optical interference.
Use the 532nm green-light source in the experiment, the 20X microcobjective.Sample is the HELE tumour cell that is placed in the common plastics double dish.The hologram that the system of Figure 2 shows that adopts, Fig. 3 is its partial enlarged drawing, can be clearly seen that the interference fringe of inclination; Fig. 4 is the phase diagram that obtains after reproducing, and can know and see the cell pattern of growing in the nutrient solution; Fig. 5 is the gray scale displayed map of Fig. 4, and gray-scale value is represented elevation information, can clearerly see the distribution of cell in the visual field.
Claims (3)
1. inversion type digital hologram microscope comprises: optical fiber collimator (1,6), optical fiber (2), fiber optic splitter (3), fiber coupler (4), laser instrument (5), CCD camera (7), close Shu Jingti (8), microcobjective (9), one dimension translation stage (10), sample stage (11), two-dimension translational platform (12), sample (13); It is characterized in that:
The place ahead of laser instrument (5) is mounted with fiber coupler (4), fiber coupler (4) links to each other with fiber optic splitter (3) by optical fiber, fiber optic splitter (3) picks out two-way optical fiber and is connected with optical fiber collimator (1) and optical fiber collimator (6) respectively, optical fiber collimator (1) below is equipped with the sample stage (11) that is used to hold sample (13), sample stage (11) is connected on (12) two-dimension translational platform, sample stage (11) below is equipped with the microcobjective (9) that is installed on the one dimension translation stage (10), microcobjective (9) is aimed at two perpendicular sides of closing Shu Jingti (8) with optical fiber collimator (6), close Shu Jingti (8) below and be equipped with CCD camera (7), CCD camera (7) links to each other with computing machine (14);
The light of laser instrument (5) outgoing is through fiber coupler (4) coupled into optical fibres, and be divided into two-way by fiber optic splitter (3): the first via is a thing light, by an optical fiber collimator (1) spherical light wave of dispersing of optical fiber outgoing is collimated into directional light, directional light is radiated on the sample (13) of horizontal positioned straight down, pass sample stage (11), microcobjective (9), and by being radiated at behind the beam cementing prism (8) on the CCD camera (7); Another road is a reference light, is shaped to directional light by optical fiber collimator (6), and level is radiated on the beam cementing prism (8), and the reflection back forms hologram by CCD camera (7) record with the thing optical interference; Above-mentioned two-way light has angle.
2. inversion type digital hologram microscope according to claim 1 is characterized in that: above-mentioned thing light is 5mm through the diameter of the directional light that optical fiber collimator (1) is collimated into.
3. inversion type digital hologram microscope according to claim 1 is characterized in that: above-mentioned reference light is 2cm through the directional light diameter that optical fiber collimator (6) is collimated into.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011200612122U CN201974574U (en) | 2011-03-09 | 2011-03-09 | Inverted digital holographic microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011200612122U CN201974574U (en) | 2011-03-09 | 2011-03-09 | Inverted digital holographic microscope |
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| Publication Number | Publication Date |
|---|---|
| CN201974574U true CN201974574U (en) | 2011-09-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011200612122U Expired - Fee Related CN201974574U (en) | 2011-03-09 | 2011-03-09 | Inverted digital holographic microscope |
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| CN (1) | CN201974574U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105974765A (en) * | 2016-05-02 | 2016-09-28 | 浙江大学 | Portable digital holographic microscopy |
| US10876970B2 (en) | 2016-04-12 | 2020-12-29 | The Board Of Regents Of The University Of Texas System | Light-sheet microscope with parallelized 3D image acquisition |
| US10989661B2 (en) | 2015-05-01 | 2021-04-27 | The Board Of Regents Of The University Of Texas System | Uniform and scalable light-sheets generated by extended focusing |
| US11340438B2 (en) * | 2016-10-25 | 2022-05-24 | Lyncee Tec Sa | Fiber splitter device for digital holographic imaging and interferometry and optical system comprising said fiber splitter device |
-
2011
- 2011-03-09 CN CN2011200612122U patent/CN201974574U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10989661B2 (en) | 2015-05-01 | 2021-04-27 | The Board Of Regents Of The University Of Texas System | Uniform and scalable light-sheets generated by extended focusing |
| US10876970B2 (en) | 2016-04-12 | 2020-12-29 | The Board Of Regents Of The University Of Texas System | Light-sheet microscope with parallelized 3D image acquisition |
| CN105974765A (en) * | 2016-05-02 | 2016-09-28 | 浙江大学 | Portable digital holographic microscopy |
| US11340438B2 (en) * | 2016-10-25 | 2022-05-24 | Lyncee Tec Sa | Fiber splitter device for digital holographic imaging and interferometry and optical system comprising said fiber splitter device |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110914 Termination date: 20130309 |