CN104232614B - Cell magnetic force micromanipulation method and system under physiological environment - Google Patents
Cell magnetic force micromanipulation method and system under physiological environment Download PDFInfo
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- CN104232614B CN104232614B CN201310254647.2A CN201310254647A CN104232614B CN 104232614 B CN104232614 B CN 104232614B CN 201310254647 A CN201310254647 A CN 201310254647A CN 104232614 B CN104232614 B CN 104232614B
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Abstract
The invention discloses cell magnetic force micromanipulation method and system under a kind of physiological environment.The system of cell magnetic force micromanipulation is in the culture environment for the suitable cell in-vitro growth that the system being made up of magnetic probe, probe nano offset detection module, three-D displacement platform, inverted/upright light microscope, magnetic probe control module and Bit andits control module is placed in accurate control under physiological environment, realizes the external operation of biological cell and real-time tracking observation.The method of cell magnetic force micromanipulation is to be based on being combined cell magnetic force micromanipulation with magnetic nano-particle, by selection region point-to-point measurement and magnetic force size is recorded to the individual cells containing magnetic nano-particle using magnetic probe, magnetic force and pattern dot matrix image are obtained in nanoscale simultaneously, it is accurately positioned magnetic cell and magnetic nano-particle, and then individual cells are operated, moved, carried, inject and tested with magnetic probe, this has directive significance to nanometer biotechnology and micro-nano manipulation technology.
Description
Technical field
The present invention relates to the cell magnetic force micromanipulation method and system under physiological environment, its potential application includes following section
Skill field：Bioscience, medical science, electronics, computer science etc..
Technical background
Nanoscale magnetic measurement is one of very important nanometer technology, and its application prospect is extremely wide, especially exists
The research of biomedical sector is significant.
Nineteen ninety, IBM Eigler etc. under the conditions of high vacuum and extremely low temperature, using PSTM 35
Xenon atom has been combined into " IBM " printed words in nickel surface, so as to realize the resettlement of atom first.Stark in 1998 etc. utilizes atom
Force microscope probe has carried out imaging and rupturing operation experiment to DNA.Kim in 2012 etc. has found that cancer is thin using AFM
The property of born of the same parents' apoptosis is relevant with surface topography roughness and rigid change, and the change of pattern and biological nature can assess anticancer
The activity of medicine.
Some domestic scientific research institutions have carried out micro-manipulating robot system and its research work in terms of biotechnology.North
Biological cell micro OS of the robot Research Institute of navigating based on optical microscopy, has carried out the base of mouse egg cell
Because injection is studied；Robot of Nankai University have developed the micro-manipulating robot towards bioengineering based on optical microphotograph vision
System, and carried out the cutting test of chromosome of wheat；Precision instrument National Key Laboratory of Tsing-Hua University also constructs laser
Microbeam operating operation test system, the optical tweezer capture for realizing particulate by accurate control trap platform position is cut with finishing tool, and is by this
System realizes the experiments such as spore operation, chromosome cutting.
Because cell there is no magnetic, and biological cell manipulate under physiological environment and certain hardly possible be present
Degree, so the magnetic research that micro-nano manipulation technology is applied to cell in vitro has not yet to see relevant report.Based on Cell magnetic
Power micromanipulation system is combined with magnetic nano-particle, and the individual cells containing magnetic nano-particle are pressed using magnetic probe
Selection region point-to-point measurement simultaneously records magnetic force size, obtains magnetic force and pattern dot matrix image simultaneously in nanoscale, is accurately positioned
Magnetic cell and magnetic nano-particle, and then individual cells are operated, moved, carried, inject and tested with magnetic probe.
Cell magnetic force micromanipulation method and system is provided directly in cell, subcellular fraction for life science under physiological environment
Or the technology and instrument of Molecular level study biological phenomena, can be provided for the diagnosis and treatment of cancer cell science it is theoretical according to
According to.
The content of the invention
It is an object of the invention to provide cell magnetic force micromanipulation method and system under physiological environment, by living to biology
Body cell is detected, manipulated to realize active somatic cell configuration, physical characteristic, dynamic process, surface in real time in growing environment
The acquisition of the information such as the distribution of acceptor and active state, field of biomedical research is provided directly in cell, subcellular fraction
And the technology and instrument of Molecular level study biological phenomena.
The present invention implementation method be：, will be by magnetic probe, probe nano offset detection module, three under physiological environment
The system that dimension displacement platform, inverted/upright light microscope, magnetic probe control module and Bit andits control module form is placed in essence
In the culture environment of the suitable cell in-vitro growth of close control, using magnetic probe to the individual cells containing magnetic nano-particle
By selection region point-to-point measurement and magnetic force size is recorded, magnetic force and pattern dot matrix image are obtained simultaneously in nanoscale, it is accurate fixed
Position magnetic cell and magnetic nano-particle, and then individual cells are operated, moved, carried, inject and surveyed with magnetic probe
Examination.
The invention has the advantages that：
Magnetic force microscopy is combined with inverted/upright light microscope can be accurately positioned magnetic cell and magnetic nano particle
Son, improve scan efficiency and scanning precision.The combination of system and cell injuring model environment to cell magnetic force micromanipulation
Use, the operation to external living cells can be achieved, obtain magnetic force and pattern dot matrix image simultaneously in nanoscale, magnetic can be used
Probe is operated to individual cells, is moved, is carried, injected and tested.
Embodiment
As shown in figure 1, to cell magnetic force micromanipulation system include by magnetic probe, probe nano offset detection module,
Three-D displacement platform, inverted/upright light microscope, magnetic probe control module, Bit andits control module and environmental control system
Composition.Liquid cell is placed on above scanner, and the cover glass with cell is placed in pond, and piezoelectric ceramic tube is alive outside
Under effect, liquid cell self-movement in the x, y, and z directions can be controlled.The laser beam that diode laser is sent passes through optics
System focuses on the micro-cantilever back side, then through in micro-cantilever backside reflection to photodetector.Due to sample surfaces atom with it is micro-
The interatomic interaction force of cantilevered probe needles, in Sample Scan, micro-cantilever will be bent with sample surface morphology
Rise and fall, the reflected beams will also be offset therewith, and then change of the can by detecting facula position obtains the surface of sample
Topographical information, meanwhile, the individual cells containing magnetic nano-particle by selection region point-to-point measurement and are remembered using magnetic probe
Magnetic force size is recorded, magnetic force and pattern dot matrix image is obtained simultaneously in nanoscale, is accurately positioned magnetic cell and magnetic nano particle
Son, and then individual cells are operated, moved, carried, inject and tested with magnetic probe.
Claims (1)
1. a kind of cell magnetic force micromanipulation method under physiological environment, it is characterised in that：It will be offset by magnetic probe, probe nano
Detection module, three-D displacement platform, inverted/upright light microscope, magnetic probe control module and Bit andits control module composition
The system suitable cell in-vitro growth that is placed in accurate control culture environment in, using magnetic probe to containing magnetic nano particle
The individual cells of son by selection region point-to-point measurement and record magnetic force size, and magnetic force and pattern dot matrix are obtained simultaneously in nanoscale
Image, magnetic cell and magnetic nano-particle are accurately positioned, and then individual cells are operated with magnetic probe, including moved
Dynamic, injection and test；
By the magnetic force of probe or additional electromagnetic force with being interacted with magnetic nano particle daughter cell, detection magnetic force size is with weight
The Distribution of Magnetic Field of structure measured zone；
Under physiological environment, cell fast rough is carried out using inverted/upright light microscope, selects magnetic imaging, operation
Target location；
The preparation method of the cell with magnetic nano-particle：It is one or more using the absorption of magnetic probe needle point, control
Magnetic nano-particle is simultaneously implanted into selected cell, obtains the cell that ad-hoc location carries magnetic nano-particle, carry out magnetic force it is micro- into
Picture and manipulation；Or co-culture cell and magnetic nano-particle, obtained by encytosis containing one or more magnetic
The cell of nano-particle distribution, carries out magnetic force micro-imaging and operation；Probe contact is selected to be connect with non-by probe control module
Tactile mode carries out micrometering and operation；
To the system of cell magnetic force micromanipulation and being used in combination for cell injuring model environment in the system, can be achieved to external
The operation of living cells, magnetic force and pattern dot matrix image are obtained simultaneously in nanoscale.
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106148185B (en) * | 2015-04-13 | 2018-11-27 | 浙江大学 | Real-time electromagnetic field cell exposure system and its application |
| CN105607686B (en) * | 2016-01-11 | 2017-02-01 | 浙江大学 | Nanometer-level-microoperation-based unicellular sub-nanometer-level motion control system |
| CN106501554B (en) * | 2016-11-16 | 2019-01-29 | 长春理工大学 | A kind of method of operating for moving magnetic nano-particle |
| CN106841684B (en) * | 2017-01-16 | 2019-05-21 | 长春理工大学 | The cooperated measuring method of cell growth state |
| CN110018332B (en) * | 2019-04-30 | 2021-08-06 | 长春理工大学 | Atomic force probe contact type scanning biological cell imaging error compensation method |
| CN112285386A (en) * | 2020-10-13 | 2021-01-29 | 长春理工大学 | Detection method of tumor exosome induced cell malignant change process based on atomic force microscopy |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010036957A1 (en) * | 2008-09-25 | 2010-04-01 | William Marsh Rice University | Systems and methods for magnetic guidance and patterning of cells and materials |
| WO2011038370A1 (en) * | 2009-09-25 | 2011-03-31 | N3D Biosciences, Inc. | Materials for magnetizing cells and magnetic manipulation |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010036957A1 (en) * | 2008-09-25 | 2010-04-01 | William Marsh Rice University | Systems and methods for magnetic guidance and patterning of cells and materials |
| WO2011038370A1 (en) * | 2009-09-25 | 2011-03-31 | N3D Biosciences, Inc. | Materials for magnetizing cells and magnetic manipulation |
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