CN201908091U - Inhaling device for picking mouse stem cell monoclone - Google Patents
Inhaling device for picking mouse stem cell monoclone Download PDFInfo
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- CN201908091U CN201908091U CN201020693501XU CN201020693501U CN201908091U CN 201908091 U CN201908091 U CN 201908091U CN 201020693501X U CN201020693501X U CN 201020693501XU CN 201020693501 U CN201020693501 U CN 201020693501U CN 201908091 U CN201908091 U CN 201908091U
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- picking
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- mouse stem
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- syringe needle
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Abstract
The utility model discloses an inhaling device for picking mouse stem cell monoclone, which relates to an inhaling device for picking steam cell monoclone and solves the problem that picking of ES cell monoclone during independent preparation of ES cells is difficult to finish. One end of a first rubber catheter of the inhaling device is an inhaling end, and the other end of the first rubber catheter is communicated with one end of a plastic catheter; the other end of the plastic catheter is communicated with one end of a second rubber catheter, and the other end of the second rubber catheter is communicated with a base body of a glass needle; the tip of the glass needle is a mouse stem cell monoclone picking end; and absorbent cotton is filled in the plastic catheter. The inhaling device for picking mouse stem cell monoclone is suitable for preparation of mouse stem cell monoclone.
Description
Technical field
The utility model relates to a kind of mouth haustorium that is used for the picking stem cell monoclonal.
Background technology
Life science is 20th century one of subjects with the fastest developing speed, has become the most noticeable field in the natural science.Stem-cell research is a big hot topic problem of 21 century life science, and the focus of research is first elected embryonic stem cell (Embryonic Stem cells, ES cell).1999 U.S. at the year end " Science " are chosen as hESC's achievement in research then first of the world's ten big science and technology progress, 2000, " Time " weekly is classified them first of 20 end of the century world's ten big technological achievements as, and thinks that embryonic stem cell and human genome will become the field of tool development of new millennium and application prospect simultaneously.Stem cell so comes into one's own and is based on its research human life's health is of great importance.
Embryonic stem cell (Embryonic Stem Cells, the ES cell) be from attached preceding body early embryo inner cell mass (ICM) or attached back archeocyte (the Primordial Germ Cells that plants of planting, PGCs) a kind of tool unlimited multiplication capacity that comes out of separating clone and the cell of omnidirectional's differentiation capability, that is: these cells can be kept the size of self cell mass by cell fission, can further break up under certain condition again simultaneously and become various histocyte, thereby constitute the histoorgan of the various complexity of body, and have the chimeric ability (comprising the reproductive tract mosaic) of formation.The ES cell is to zoologize the ideal model of developmental biology basic problems such as embryo's generation in early days, cytodifferentiation, gene regulating, also is the important tool of organizational project, pharmacology and clinic study.At present, independent preparation ES cell faces many technological difficulties in the laboratory, and one of them is: inner cell mass forms monoclonal picking.
The utility model content
The utility model is to be difficult to finish the problem of picking ES cell monoclonal in order to solve in the process of preparation ES cell, thereby a kind of monoclonal mouthful of haustorium of picking mouse stem cells that be used for is provided.
A kind of monoclonal mouthful of haustorium of picking mouse stem cells that be used for, it comprises rubber catheter, plastic catheter, No. two rubber catheters and glass syringe needle, one end of a described rubber catheter is a mouthful suction end, the other end of a described rubber catheter is communicated with an end of plastic catheter, the other end of described plastic catheter is communicated with an end of No. two rubber catheters, the other end of No. two rubber catheters is communicated with the matrix of glass syringe needle, and the needle point of glass syringe needle is the monoclonal picking end of mouse stem cells; Fill absorbent cotton in the described plastic catheter.
The utility model stretches into the needle point of glass syringe needle the picking of realization clone in the mouse stem cells group in use.The utility model is simple to operate, and it is higher to draw success ratio.
Description of drawings
Fig. 1 is a structural representation of the present utility model; Fig. 2 is the structural representation of embodiment three.
Embodiment
Embodiment one, in conjunction with Fig. 1 this embodiment is described, a kind of monoclonal mouthful of haustorium of picking mouse stem cells that be used for, it comprises rubber catheter 1 No. one, plastic catheter 2, No. two rubber catheters 3 and glass syringe needle 4, one end of a described rubber catheter 1 is a mouthful suction end, the other end of a described rubber catheter 1 is communicated with an end of plastic catheter 2, the other end of described plastic catheter 2 is communicated with an end of No. two rubber catheters 3, the other end of No. two rubber catheters 3 is communicated with the matrix 41 of glass syringe needle 4, and the needle point 42 of glass syringe needle 4 is the monoclonal picking ends of mouse stem cells; Fill absorbent cotton in the described plastic catheter 2.
In the present embodiment, the needle point of glass syringe needle 4 burns 2-3 second at the spirit lamp flame envelope in preparation process, make its opening end level and smooth, avoids scratching cell.
Carry out mouse embryo stem cell (ES) mono-clonal picking method and comprise following operation:
1, raise the preparation of individual layer:
(1) MEF after freezing (mouse embryo fibroblasts make feeder layer cells) is thawed and be cultured to 80%～90% (confluent culture ware bottom), change the nutrient solution that contains 10 μ g/ml ametycins into, in incubator, act on 2～5 hours.
(2) covered the cultivation plate hole 0.5 hour with gelatin in advance, inhale before using and remove gelatin.Remove the ametycin in the feeder layer cells nutrient solution, trysinization, centrifugal 5 minutes of 1000rp, and being inoculated in the cultivation plate hole of using the gelatin shop fixtures in advance is that 37 ℃, concentration are 5% CO in temperature
2Cultivate in the incubator of saturated humidity down.
2, mouse embryo stem cell separates
(1) experiment embryo's acquisition
(1) afternoon, 15:00 selected 10 female kunming mices, and abdominal injection PMSG10IU/ only, and 48h pneumoretroperitoneum HCG injection 10IU/ only mates with male mouse simultaneously at 1: 1, checked cloudy bolt and was labeled as 0.5dPc about 8:00 morning next day.
After (2) 3.5 days mouse cervical vertebra dislocation method is put to death, open the abdominal cavity then, expose the uterus, microscopically utilization operation liquid (PBI) flushing uterus obtains blastaea.
(2) separation method
(1) embryo that blister cavities is fully expanded is to handle about 30s in 2 the acid solution at PH, removes zona pellucida, and cleaning will go the blastaea of zona pellucida to be transplanted on the raising individual layer for preparing then in the phosphate buffered saline buffer (PBS) of no calcium ions and magnesium ions, change the ES substratum.
The embryo is adherent about (2) 48 hours, and trophocyte's spread apart is also attaching the culture dish surface growth, and inner cell mass is positioned at the central part of trophocyte's layer, upwards protuberance growth.
(3) continue to cultivate 2 to 4 days, inner cell mass continues to increase, and observes when inner cell mass obviously swells growth and utilizes a mouthful suction pipe that it is chosen.
(4) provoke inner cell mass with the capillary needle of the big slightly self-control mouth haustorium of terminal relative aperture inner cell agglomerate, be drawn in the Digestive system droplet.
(5) inner cell mass is drawn to the Digestive system droplet one by one, at room temperature acts on 1 to 3 minute.Use the capillary needle of the little self-control mouth haustorium of terminal relative aperture inner cell mass instead, inhale gently and blow the inner cell agglomerate, make it to be dispersed to 3 to 4 cells little agglomerate together.
(6) dispersive inner cell mass fritter is drawn on the raising individual layer of freshly prepd interpolation ES substratum and cultivates, can obtain mouse embryo stem cell (ES) clone in 3-4 days.
Embodiment two, this embodiment and embodiment one described a kind of difference that is used for monoclonal mouthful of haustorium of picking mouse stem cells are, it also comprises suction nozzle 5, described suction nozzle 5 is one section plastics tubing, and described suction nozzle 5 is inhaled end with the mouth of a rubber catheter 1 and is communicated with.
Embodiment three, this embodiment is described in conjunction with Fig. 2, this embodiment is that with embodiment one or two described a kind of differences that are used for monoclonal mouthful of haustorium of picking mouse stem cells the angle between the needle point 42 of glass syringe needle 4 and the matrix 41 of glass syringe needle 4 is 90 °～180 °.
More convenient being deep into of present embodiment drawn the clone that inner cell mass forms in the culture dish.
Embodiment four, this embodiment and embodiment one described a kind of difference that is used for monoclonal mouthful of haustorium of picking mouse stem cells are that the angle between the needle point 42 of glass syringe needle 4 and the matrix 41 of glass syringe needle 4 is 120 °.
Claims (4)
1. one kind is used for monoclonal mouthful of haustorium of picking mouse stem cells, it is characterized in that: it comprises a rubber catheter (1), plastic catheter (2), No. two rubber catheters (3) and glass syringe needle (4), one end of a described rubber catheter (1) is a mouthful suction end, the other end of a described rubber catheter (1) is communicated with an end of plastic catheter (2), the other end of described plastic catheter (2) is communicated with an end of No. two rubber catheters (3), the other end of No. two rubber catheters (3) is communicated with the matrix (41) of glass syringe needle (4), and the needle point (42) of glass syringe needle (4) is the monoclonal picking end of mouse stem cells; Fill absorbent cotton in the described plastic catheter (2).
2. a kind of monoclonal mouthful of haustorium of picking mouse stem cells that be used for according to claim 1 is characterized in that it also comprises suction nozzle (5), and described suction nozzle (5) is one section plastics tubing, and described suction nozzle (5) is inhaled end with the mouth of a rubber catheter (1) and is communicated with.
3. a kind of monoclonal mouthful haustorium of picking mouse stem cells that is used for according to claim 1 and 2 is characterized in that the angle between the matrix (41) of the needle point (42) of glass syringe needle (4) and glass syringe needle (4) is 90 °～180 °.
4. a kind of monoclonal mouthful haustorium of picking mouse stem cells that is used for according to claim 3 is characterized in that the angle between the matrix (41) of the needle point (42) of glass syringe needle (4) and glass syringe needle (4) is 120 °.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201020693501XU CN201908091U (en) | 2010-12-31 | 2010-12-31 | Inhaling device for picking mouse stem cell monoclone |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201020693501XU CN201908091U (en) | 2010-12-31 | 2010-12-31 | Inhaling device for picking mouse stem cell monoclone |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201908091U true CN201908091U (en) | 2011-07-27 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201020693501XU Expired - Fee Related CN201908091U (en) | 2010-12-31 | 2010-12-31 | Inhaling device for picking mouse stem cell monoclone |
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| CN (1) | CN201908091U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104593250A (en) * | 2015-01-06 | 2015-05-06 | 长沙赢润生物技术有限公司 | Flexible single-cell separator and separation culture technology |
| CN105675378A (en) * | 2014-11-18 | 2016-06-15 | 上海张江转化医学研发中心有限公司 | Simple single circulating tumor cell separation method and apparatus |
| CN105831105A (en) * | 2016-04-12 | 2016-08-10 | 上海理工大学 | Microfluid cell processing chip and application method thereof |
| CN108034580A (en) * | 2017-11-13 | 2018-05-15 | 广东艾时代生物科技有限责任公司 | A kind of method that pin picking multipotential stem cell clone is drawn using glass |
-
2010
- 2010-12-31 CN CN201020693501XU patent/CN201908091U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105675378A (en) * | 2014-11-18 | 2016-06-15 | 上海张江转化医学研发中心有限公司 | Simple single circulating tumor cell separation method and apparatus |
| CN104593250A (en) * | 2015-01-06 | 2015-05-06 | 长沙赢润生物技术有限公司 | Flexible single-cell separator and separation culture technology |
| CN105831105A (en) * | 2016-04-12 | 2016-08-10 | 上海理工大学 | Microfluid cell processing chip and application method thereof |
| CN105831105B (en) * | 2016-04-12 | 2018-12-14 | 上海理工大学 | Microfluidic cell handles chip and its application method |
| CN108034580A (en) * | 2017-11-13 | 2018-05-15 | 广东艾时代生物科技有限责任公司 | A kind of method that pin picking multipotential stem cell clone is drawn using glass |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110727 Termination date: 20121231 |