CN201876403U - Particle-size in-situ measuring device for phytoplankton - Google Patents
Particle-size in-situ measuring device for phytoplankton Download PDFInfo
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- CN201876403U CN201876403U CN2010205353609U CN201020535360U CN201876403U CN 201876403 U CN201876403 U CN 201876403U CN 2010205353609 U CN2010205353609 U CN 2010205353609U CN 201020535360 U CN201020535360 U CN 201020535360U CN 201876403 U CN201876403 U CN 201876403U
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Abstract
The utility model provides a particle-size in-situ measuring device for phytoplankton in water. The device consists of an underwater optical measuring device and an overwater optical fiber coupling device as well as a data acquiring and controlling device. The laser can be guided in the underwater optical measuring device by single-mode fiber; fluorescent signals and differential Doppler signals emitted by the phytoplankton are received respectively at two sides of two intervened and crossed laser beams in the direction forming 90 degrees with the transmission direction of the laser beams, and are respectively transmitted to an overwater photoelectric detecting unit and a data acquiring card by the single-mode fiber; and the program in a computer automatically memorizes and displays a particle-size statistic distribution curve and the information such as average flowing speed, flow and the like in the statistics in real time. The measurement of the device is not affected by the water flow speed; the underwater optical measuring device completely adopts the optical device; and a measuring signal is transmitted by the optical fiber, the power supply is not required, therefore, the device is suitable for in-situ measuring the particle-size distribution and particle concentration of the phytoplankton for a long time.
Description
Technical field
The utility model relates to a phytoplankton grain diameter measurement device, and this device is applied to phytoplankton grain size in the in-site measurement seawater, realizes the real-time monitoring of size distribution and particle concentration.
Background technology
At present, phytoplankton grain diameter measurement method mainly contains microscope count method, image analytical method, Ku Erte counting method, flow cytometry method.Microscope count method is size, the shape at microscopically observation phytoplankton cell; Image analytical method is according to cell geometric shape information, utilizes software for calculation to calculate the phytoplankton particle diameter; The Ku Erte counting method is to utilize the phytoplankton cell when being arranged in the measurement aperture of continuous current electrode system, the resistance of mutagenesis increase and corresponding electrode between potential difference (PD) change, calculate the particle diameter of phytoplankton by the variation of potential difference (PD).Above-mentioned three kinds of measuring methods all need complicated seawater sample preprocessing process, and not competent scene is measured in real time.The flow cytometry method utilizes contained chlorophyll fluorescence of phytoplankton and scattering to analyze the optical signature of phytoplankton, and the particle diameter of phytoplankton cell adopts the experimental formula between forward scattering and cell volume to obtain.This method requires the positional precision of tested cell by observation point less than 1 μ m, and needs stable flow velocity, so this method also is difficult to be applied to in-site measurement.
Doctor Chen Qiyao of Hong Kong Baptist University has proposed double beams laser fluorescence Doppler method, and this method is that the particle diameter method of phytoplankton is measured in the combination of using laser excitation chlorophyll fluorescence and two kinds of technology of laser differential Doppler range rate measurement.Specifically be to measure the speed of phytoplankton particle flux, obtain particle by fluorescence signal and cross tested volume required time, thereby calculate the size of the phytoplankton particle diameter of the tested water body of flowing through through tested water body by Doppler signal.Irrelevant because of the measurement of particle diameter with refractive index and fluorescence intensity, and do not require constant flow velocity, make this method be suitable for in-site measurement.
Summary of the invention
The purpose of this utility model is to adopt double beams laser fluorescence Doppler method, provides a kind of scene to measure the phytoplankton particle diameter in real time, thus the device of phytoplankton size distribution and particle concentration in the statistics continuous monitoring seawater automatically.
This device comprises laser instrument, laser fiber coupling device waterborne, underwater optics measurement mechanism, data acquisition waterborne and control device.Laser fiber coupling device waterborne will be imported Laser Transmission optical measuring device extremely under water by single-mode fiber, and the light signal of underwater optics measurement mechanism output transfers to data acquisition waterborne and control device by multimode optical fiber.
The underwater optics measurement mechanism comprises input laser signal receiving trap, output fluorescence signal receiving trap and doppler difference signal receiving device three parts.In the input laser signal receiving trap collimation lens, beam splitting arrangement and condenser lens are arranged,, form interference fringe, constitute measured zone laser signal collimation, beam split, the focusing of input.In output fluorescence signal receiving trap and the doppler difference signal receiving device condenser lens is arranged all, measuring-signal is imaged in the multimode optical fiber.The optical measurement probe is sealed in the stainless steel rectangular parallelepiped frame seal cabin fully, sinks under the water fully during measurement.
The photodetector unit that data acquisition waterborne and control device are made of photomultiplier, form based on pci data capture card and the computing machine of DSP.Program in data collecting card and the computing machine makes device can carry out the statistics of phytoplankton size distribution by the preheating setting time Automatic Cycle, and stores automatically and information such as the mean flow rate that shows statistical distribution curve and this time statistics in real time, flow.Can realize monitoring in real time for a long time, whether finish by external key control observation process.
Underwater optics measurement mechanism of the present utility model adopts optical texture fully, and transmission waterborne, underwater signal adopts single mode and multimode optical fiber to finish, and optical measuring device does not under water need power supply fully, can work long hours under water; Employing based on DSP pci data collection and control device, guaranteed the real-time of data acquisition and processing (DAP).
Description of drawings
Accompanying drawing is the general structure synoptic diagram of device.
Wherein, 1. laser instrument 2. coupling devices 3. lens 4. fiber connectors 5. single-mode fibers 6. are imported laser signal receiving traps 7. collimation lenses 8. laser beam splitter devices 9. condenser lenses 10. doppler difference signal receiving devices 11. measurement volumes 12. fluorescence signal receiving traps 13. pressurized capsules 14. multimode optical fibers 15. data acquisition control devices 16. photodetector unit 17. data collecting cards 18. computing machines
Embodiment
This measurement mechanism divides waterborne and two parts under water.Above water is made up of laser instrument 1, coupling device 2 and data acquisition control device 15; Underwater portion is sealed in the optical measuring device of being made up of input laser signal receiving trap 6, fluorescence signal receiving trap 12 and doppler difference signal receiving device 10 in the pressurized capsule 13.Respectively by single-mode fiber 5 and multimode optical fiber 14 transmission input and output light signals.
Optical measurement probe under water is sealed in the probe pressurized capsule 13 fully, and probe pressurized capsule 13 adopts stainless steel frame, and shell is rectangular parallelepiped, has the rectangular cylindrical shell of hollow in the middle of the pressurized capsule, the glass window of wall processing symmetry, the complete submerged of device during measurement.
During measurement, the single mode Gaussian beams of the 532nm that laser instrument 1 produces, the coupling adjusting gear of forming via lens 3 and fiber connector 42 is coupled to single-mode fiber 5, will import Laser Transmission to importing laser signal receiving trap 6 under water through single-mode fiber 5.Import under water in the laser signal receiving trap 6, the laser of input is by collimation lens 7 collimations, be divided into two collimation coherent laser outgoing beams through laser beam splitter device 8 again, this two bundles coherent light beam is focused on by condenser lens 9, intersect in the measurement water body of permeametry glass window outside pressurized capsule 13, the intersection region that forms interference fringe constitutes measures water volume 11.On the direction that departs from incoming laser beam 90 degree, receive the fluorescence excitation signal by fluorescence signal receiving trap 12, simultaneously, becoming 90 opposite sides of spending to be with incident beam and accepting on the opposite direction of fluorescence signal by output doppler difference signal receiving device 10 reception laser differential Doppler signals.Fluorescence signal and doppler difference signal are sent to data acquisition control device 15 waterborne respectively through multimode optical fiber 14 and carry out collection, processing and the control of data.
Data acquisition control device 15 waterborne is made of photodetector unit 16, data collecting card 17 and computing machine 18.The photodetector unit 16 that fluorescence signal that multimode optical fiber 14 transmission come and doppler difference signal are made of photomultiplier is respectively carried out the photosignal conversion, is amplified, be input to pci data capture card 17 image data based on DSP, the data of gathering are imported computing machine, are carried out the storage and the data processing of data by computing machine.
During device work, the doppler difference signal and the laser fluorescence signal of collection are synchronous in time, and the laser fluorescence signal is used for the triggering of data acquisition.The pre-trigger data acquisition cycling of 2K is applied to doppler difference signal and laser fluorescence signals collecting simultaneously.The laser fluorescence signal and the preset threshold that collect compare in real time, in case find the laser fluorescence signal greater than the threshold value that sets in advance, the data collecting card of laser fluorescence signal will trigger the collection that doppler difference signal data acquisition card is finished follow-up 6K doppler difference signal and laser fluorescence signal simultaneously.The total amount of sampled data comprises that the 2K data of pre-triggering are 8K.In a single day data acquisition is finished, and a DSP on the data collecting card will carry out to the doppler difference signal data that collects fast that Fourier transform obtains laser Doppler frequencies.Simultaneously, the peak value of detection laser fluorescence signal, and then obtain the time width of pulse, this pulse width just the phytoplankton particle through measuring the time t of water body.By the phytoplankton particle rapidity V that Doppler frequency is extrapolated, by formula D=Vt-W calculates the particle diameter of phytoplankton particle, and wherein W is the width of measurement volumes 11 place's laser beam.Information such as the Automatic Program storage in the computing machine and the mean flow rate of real-time demonstration particle diameter statistical distribution curve and this time statistics, flow.Realize monitoring in real time for a long time, whether finish by external key control observation process.
Use the utility model, can realize phytoplankton grain size in-site measurement, phytoplankton size distribution and particle concentration in the real-time continuous monitoring seawater.
Claims (7)
1. phytoplankton particle diameter field measurement apparatus in the water, it comprises laser instrument (1), laser fiber coupling device waterborne (2), underwater optics measurement mechanism (13) and data acquisition waterborne and control device (15); It is characterized in that the underwater optics measurement mechanism comprises input laser signal receiving trap (6), output doppler difference signal receiving device (10) and fluorescence signal receiving trap (12), adopts optical device to measure fully; To under water, receive fluorescence signal and the doppler difference signal of measuring by single-mode fiber (5) transmission incident laser signal by multimode optical fiber (14).
2. measurement mechanism according to claim 1 is characterized in that the input laser signal receiving trap (6) in the underwater optics measurement measurement mechanism (13) is made up of collimation lens (7), beam splitting arrangement (8) and condenser lens (9).
3. measurement mechanism according to claim 1 and 2 is characterized in that beam splitting arrangement (8) is the integral structure that is made of the parallel tetragonal body glass of horizontal beam splitter.
4. measurement mechanism according to claim 1 is characterized in that selecting for use wavelength 532nm, the single longitudinal mode laser of power 150mw.
5. measurement mechanism according to claim 1, the reciever that it is characterized in that exporting fluorescence signal be to for becoming 90 degree with the laser beam working direction of incident, the reciever of output doppler difference signal to the reciever of fluorescence signal to opposite.
6. measurement mechanism according to claim 1 is characterized in that being that underwater optics measurement mechanism (13) is sealed in the stainless steel rectangular parallelepiped frame seal cabin fully.
7. measurement mechanism according to claim 1, it is characterized in that data acquisition waterborne and control device (15) carry out the statistics of phytoplankton size distribution by the preheating setting time Automatic Cycle, and store and show in real time mean flow rate, the flow information of statistical distribution curve and this time statistics automatically.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205353609U CN201876403U (en) | 2010-09-17 | 2010-09-17 | Particle-size in-situ measuring device for phytoplankton |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205353609U CN201876403U (en) | 2010-09-17 | 2010-09-17 | Particle-size in-situ measuring device for phytoplankton |
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| Publication Number | Publication Date |
|---|---|
| CN201876403U true CN201876403U (en) | 2011-06-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2010205353609U Expired - Fee Related CN201876403U (en) | 2010-09-17 | 2010-09-17 | Particle-size in-situ measuring device for phytoplankton |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103901438A (en) * | 2014-04-18 | 2014-07-02 | 中国科学院半导体研究所 | Optical stereo sampling in-situ detection method capable of acquiring zooplankton information |
| CN108088835A (en) * | 2018-02-06 | 2018-05-29 | 清华大学深圳研究生院 | A kind of underwater detecting system based on laser induced breakdown spectroscopy |
-
2010
- 2010-09-17 CN CN2010205353609U patent/CN201876403U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103901438A (en) * | 2014-04-18 | 2014-07-02 | 中国科学院半导体研究所 | Optical stereo sampling in-situ detection method capable of acquiring zooplankton information |
| CN103901438B (en) * | 2014-04-18 | 2016-03-09 | 中国科学院半导体研究所 | A kind of light solid sampling in-situ investigation method realizing animal plankton acquisition of information |
| CN108088835A (en) * | 2018-02-06 | 2018-05-29 | 清华大学深圳研究生院 | A kind of underwater detecting system based on laser induced breakdown spectroscopy |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110622 Termination date: 20110917 |