CN201859147U - Test strip for detecting Newcastle disease and infectious bursal disease virus in one step - Google Patents
Test strip for detecting Newcastle disease and infectious bursal disease virus in one step Download PDFInfo
- Publication number
- CN201859147U CN201859147U CN 201020206457 CN201020206457U CN201859147U CN 201859147 U CN201859147 U CN 201859147U CN 201020206457 CN201020206457 CN 201020206457 CN 201020206457 U CN201020206457 U CN 201020206457U CN 201859147 U CN201859147 U CN 201859147U
- Authority
- CN
- China
- Prior art keywords
- antibody
- infectious bursal
- newcastle disease
- bursal disease
- virus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Abstract
The utility model provides a test strip for detecting a Newcastle disease and an infectious bursal disease virus in one step, which can solve the problem that the Newcastle disease and the infectious bursal disease virus cannot be simultaneously detected in the prior art. The test strip comprises a bottom lining; a coating film is pasted in the middle of the upper face of the bottom lining; the middle of the coating film is provided with a detection coating an antibody of the Newcastle disease, a detection line coating the antibody of the infectious bursal disease virus and a control line coating an anti-mouse IgG antibody; a glass fiber film coated with a labelled antibody and absorbent paper are respectively pasted at the left side and the right side of the upper face of the coating film; and a sample pad is pasted on the glass fiber film. The test strip can detect the Newcastle disease and the infectious bursal disease virus in one step, has the advantages of strong specificity, high sensitivity, high detection speed and low cost and is simple and convenient to operate and can be widely applied to farmers, raising households, grass roots and individuals to detect the Newcastle disease and the infectious bursal disease virus.
Description
Technical field
The utility model belongs to Preventive Veterinary Medicine check field, specifically relates to the test strips of an a kind of step detection newcastle disease and infectiousness bursal disease poison.
Background technology
(Newcastle Disease ND) is the eqpidemic disease of bringing great harm to poultry husbandry to newcastle disease always, is classified as the category-A infectious disease by World Organization for Animal Health (OIE), and this disease has bigger business impact to world commerce.Gumboro disease (IBD) is a kind of acute height contagious disease, and this disease is the strongest to chick or growing chickens neurological susceptibility, and infection rate can reach 100%, and mortality ratio is than higher, and the chicken group just causes large tracts of land death once infection, causes the tremendous economic loss to the raiser.Have a strong impact on the development of poultry husbandry.At present, some new variations have taken place in the outburst of chicken disease, the situation of a certain disease of the sort of in the past simple generation is rare, the substitute is several eqpidemic disease mixed infections, viral blight, recessive chronic disease increasing day by day, the incidence of disease of respiratory diseases has surpassed the enteron aisle disease. and these all make sick increasingly sophisticatedization of chicken, bring certain degree of difficulty for the sick diagnosis and treatment of chicken, particularly newcastle disease and infectiousness French capsule, the clinical symptoms of sick chicken has very big difference, different hosts are also different to metainfective reaction, this makes diagnosis more complicated and difficult, and single clinical and pathology can not be as the foundation of diagnosis.
Detection method to avian viruses, mainly containing virus separates, serodiagnosis and molecular biology method, but viral disengaging time is long, the requirement condition height, separation rate is low, and gather the patients acuity phase and convalescence paired sera carry out TPPA and confirm, can not in time diagnose again, therefore, these two kinds of methods in use are very restricted, and immune-gold labeled method (Immunochromatography Assay) grows up the early 1990s, and is quick because of it, easy and simple to handle, stable reagent, but room temperature accumulating, being difficult for pollution characteristics is widely used.It is the combination of immune affine technology, marking technology, immunolabelling technique and chromatographic technique.With coated antibody, colloid gold label antibody immobilization, combine with sample sorbing material etc., prepare the immunochromatography diagnostic test, only need insert sample solution to this strip down during use, several minutes just can judged result.Compare with the immunity percolation method, good stability, operate easier, detect quicker, and owing to be dried strip form, need not cryopreservation, accumulating is also convenient, if the method for the detection avian viruses of strip form can be spread in ground peasant household, raiser, basic unit and the staff, make it find eqpidemic disease as early as possible, can access timely and effective processing, so that reduce economic loss.
But at present also not about detecting the device or the testing tool of newcastle disease and infectiousness bursal disease poison simultaneously.
Summary of the invention
The utility model provides an a kind of step to detect the test strips of newcastle disease and infectiousness bursal disease poison, can solve the problem that can not detect newcastle disease and infectiousness bursal disease poison simultaneously that prior art exists.Test strips of the present utility model can a step detect newcastle disease and infectiousness bursal disease poison.
For solving the problems of the technologies described above, the utility model is achieved by the following technical solutions:
An a kind of step is detected the test strips of newcastle disease and infectiousness bursal disease poison, comprise end liner, obedient coated film is glued at middle part above the described end liner, described coated film middle part have a bag by the detection of antibodies line of newcastle disease virus, bag by the detection of antibodies line of avian infectious bursal disease poison and a bag by the control line of anti-mouse IgG antibody, the glass fibre membrane and the thieving paper of sticking obedient coated with gold labeling antibody respectively about described coated film top are pasted sample pad above the described glass fibre membrane.
Test strips of the present utility model is utilized modern international state-of-the-art colloidal gold immunochromatographimethod technology, by bag is set simultaneously on test strips by the detection of antibodies line of newcastle disease virus, bag by the detection of antibodies line of avian infectious bursal disease poison and bag by the control line of anti-mouse IgG antibody, can synchronous detection newcastle disease virus and avian infectious bursal disease poison, be ground peasant household, the raiser, basic unit and individual test oneself etc. a kind of test strips that detects newcastle disease and infectiousness bursal disease poison simultaneously are provided, this test strips has high specificity, highly sensitive, the advantage that detection speed is fast, and need not use instrument and equipment, with low cost, easy and simple to handle, can be widely used in ground peasant household, the raiser, basic unit and individual are for the detection of newcastle disease virus and avian infectious bursal disease poison, for in time finding effectively to detect epidemic situation and lay a good foundation.
Further, described bag is in monoclonal antibody, the polyclonal antibody one or both by the antibody of newcastle disease virus.
Further, described bag is in monoclonal antibody, the polyclonal antibody one or both by the antibody of avian infectious bursal disease poison.
Further, monoclonal antibody by the anti-newcastle disease virus of colloid gold particle mark and anti-avian infectious bursal disease poison is arranged in the glass fibre membrane of described coated with gold labeling antibody.
The preparation method of above-mentioned test strips comprises the steps:
1) prepares coated film earlier, be cushioned monoclonal antibody or the polyclonal antibody and the anti-mouse IgG antibody of the monoclonal antibody of liquid dilution anti-newcastle disease or polyclonal antibody, anti-avian infectious bursal disease poison with bag, and three kinds of antibody after will diluting are sprayed on the middle part of nitrocellulose membrane respectively abreast, form the detection line of avian infectious bursal disease poison, the detection line of newcastle disease virus and the control line of anti-mouse IgG respectively after three kinds of antibody infiltrate nitrocellulose membrane, make coated film;
2) prepare the glass fibre membrane that is coated with the deposit labeling antibody again;
3) coated film is sticked on middle part above the end liner, on coated film about the glass fibre membrane and the thieving paper of sticking obedient coated with gold labeling antibody respectively;
4) paste sample pad above the glass fibre membrane of coated with gold labeling antibody, make big plate, cutting promptly gets colloidal gold strip.
The application of above-mentioned test strips in a step detection newcastle disease and infectiousness bursal disease poison.
Description of drawings
Fig. 1 is the cross-sectional configuration synoptic diagram of test strips of the present utility model;
Wherein:
1, end liner; 2, sample pad; 3, glass fibre membrane; 4, coated film; 5, thieving paper; 6, bag is by the detection of antibodies line of newcastle disease virus; 7, bag is by the detection of antibodies line of avian infectious bursal disease poison; 8, bag is by the control line of anti-mouse IgG antibody.
Embodiment
Below in conjunction with the drawings and specific embodiments the utility model is described in further detail.
As shown in Figure 1, an a kind of step is detected the test strips of newcastle disease and infectiousness bursal disease poison, comprise end liner 1, the sticking obedient coated film 4 in middle part above the end liner 1, coated film 4 middle parts have a bag by the detection of antibodies line 6 of newcastle disease virus, bag by the detection of antibodies line 7 of avian infectious bursal disease poison and a bag by the control line 8 of anti-mouse IgG antibody, the glass fibre membrane 3 and the thieving paper 5 of sticking obedient coated with gold labeling antibody respectively about coated film 4 top are pasted sample pad 2 above the glass fibre membrane 3.
Test strips of the present utility model can be used for a step detection newcastle disease virus and an avian infectious bursal disease poison.
Wherein, bag is in monoclonal antibody, the polyclonal antibody one or both by the antibody of newcastle disease virus; Bag is in monoclonal antibody, the polyclonal antibody one or both by the antibody of avian infectious bursal disease poison.
Monoclonal antibody by the anti-newcastle disease virus and the anti-avian infectious bursal disease poison of colloid gold particle mark is arranged in the glass fibre membrane of coated with gold labeling antibody.
The concrete preparation method of colloidal gold strip is as follows:
The preparation of the cell strain of monoclonal antibody of Avian pneumo-encephalitis virus and calibrating:
Slowly stir the chick embryo allantoic liquid that contains Avian pneumo-encephalitis virus, and add sodium chloride, making its final concentration is 0.5mol/L, add the 10%(percent by weight again, Macrogol 6000 (PEG6000) down together), 4 ℃ are spent the night, centrifugal 30 minutes of 8000r/min, collect the virus precipitation, with phosphate buffer (PBS) dissolving, 4 ℃ are spent the night with virus precipitation, with 10000r/min centrifugal 1 hour, the gained precipitation is concentrated virus, is stored in-20 ℃ of low temperature refrigerators standby.
Take out the viruses that concentrate from-20 ℃ of low temperature refrigerators, dissolve the back and give BALB/C mice multiple spot hypodermic injection (0 .5ml/ only), 15 days at interval, immunity was 3 times altogether, merged preceding 3 days, attacked with the antigen amount of above-mentioned concentrated virus 0.25 ml at mouse peritoneal.With containing the 10%(percent by volume, down with) DMEM of calf serum cultivates the lymphocyte of the BALB/C mouse of SP2/0 cell and immunity, respectively by 2 * 10
7With 2 * 10
8Ratio merges with polyethylene glycol 1500 (PEG1500), the supernatant in the fusion hole is added in the antigen coated tygon of Avian pneumo-encephalitis virus 96 holes respectively pulls, and detects with the ELISA method, determines the cell positive hole.The positive strain that detects gained is carried out subclone with limiting dilution assay, with the monoclonal antibody cell line of subclone gained in the external cultivation of going down to posterity, and carry out repeatedly liquid nitrogen cryopreservation and recovery, produce Avian pneumo-encephalitis virus monoclonal antibody positive rate 100%, and keep the ability of stably excreting antibody, its ELISA tires and reaches more than the 1:1000.Every mouse peritoneal injecting fluid paraffin 0.5ml under aseptic condition, every the mouse peritoneal injection in a week back enlarged culture is that 0.2ml contains 1-3 * IO
6Individual hybridoma.Injection cell line after 7-10 days, or mouse once gathers ascites before dying, preserves down for-20 ℃.Adopt ammonium sulfate precipitation method slightly to carry, and then,, only show single protein band through the calibrating of SDS-PAGE electrophoresis with HITraprProteinA post purifying.Adopt the experiment of NaSCN competitive ELISA, measure its effective dose 50 (ED
50) drop-out value, reflect the size of its antibody affinity indirectly.Select wherein affinity cell line preferably, detect with the antigen binding site of addition ELISA method to it.
The preparation and the calibrating of the cell strain of monoclonal antibody of infectiousness bursal disease poison:
According to infectiousness bursal disease viral disease poison vp2 sequence construct expression vector, induce the inclusion body that produces vp2 albumen, broken purifying obtains highly purified infectiousness bursal disease viral disease poison vp2, is stored in-20 ℃ of low temperature refrigerators standby.
Take out the viruses that concentrate from-20 ℃ of low temperature refrigerators, dissolve the back and give BALB/C mice multiple spot hypodermic injection (0 .5ml/ only), 15 days at interval, immunity was 3 times altogether, merged preceding 3 days, attacked with the antigen amount of above-mentioned concentrated virus 0.25 ml at mouse peritoneal.With DMEM cultivation SP2/0 cell that contains 10% calf serum and the lymphocyte of the BALB/C mouse of immunity, respectively by 2 * 10
7With 2 * 10
8Ratio merges with polyethylene glycol 1500 (PEG1500), the supernatant in the fusion hole is added in antigen coated tygon 96 holes of avian infectious bursal disease poison respectively pulls, and detects with the ELISA method, determines the cell positive hole.The positive strain that detects gained is carried out subclone with limiting dilution assay, with the monoclonal antibody cell line of subclone gained in the external cultivation of going down to posterity, and carry out repeatedly liquid nitrogen cryopreservation and recovery, produce avian infectious bursal disease poison monoclonal antibody positive rate 100%, and keep the ability of stably excreting antibody, its ELISA tires and reaches more than the 1:1000.Every mouse peritoneal injecting fluid paraffin 0.5ml under aseptic condition, every the mouse peritoneal injection in a week back enlarged culture is that 0.2ml contains 1-3 * IO
6Individual hybridoma.Injection cell line after 7-10 days, or mouse once gathers ascites before dying, preserves down for-20 ℃.Adopt ammonium sulfate precipitation method slightly to carry, and then,, only show single protein band through the calibrating of SDS-PAGE electrophoresis with HITraprProteinA post purifying.Adopt the experiment of NaSCN competitive ELISA, measure its ED
50Drop-out value, reflect the size of its antibody affinity indirectly.Select wherein affinity cell line preferably, detect with the antigen binding site of addition ELISA method to it.
The preparation of coated film:
Bag is cushioned the preparation of liquid: with the carbonate buffer solution (PBS) of 0.05M, pH9.6, use the 0.22u membrane filtration, place 4 ℃ standby, the term of validity is 7 days.
The sealing of the preparation of damping fluid: with the PBS of 0.01M, pH7.0, with 0.22u membrane filtration mistake, place 4 ℃ standby, the term of validity is 7 days.
The preparation of sealing working fluid: will contain the PBS of 2%BSA, 2% skimmed milk, 0.01M, pH7.0, with 0.22u membrane filtration mistake, place 4 ℃ standby, the term of validity is 3 days.
The preparation of the detection line 6 of anti-newcastle disease virus: debugging Membrane jetter, spouting liquid is 25 microlitres/35 centimetre, is cushioned the monoclonal antibody of liquid dilution anti-newcastle disease virus with bag, and concentration is 70ug/ml, line on the nitrocellulose membrane middle part, room temperature was dried 20 minutes.
The preparation of infections chicken cloacal bursa virus resisting detection line 7: debugging Membrane jetter, spouting liquid is 25 microlitres/35 centimetre, be cushioned the monoclonal antibody of liquid dilution infections chicken cloacal bursa virus resisting with bag, concentration is 70ug/ml, line on the nitrocellulose membrane middle part, this line is parallel with anti-newcastle disease virus detection line 6, and line-to-line is every 5mm, should be careful even, room temperature was dried 20 minutes.
The preparation of control line 8: debugging Membrane jetter, spouting liquid is 25 microlitres/35 centimetre, be cushioned liquid with bag and dilute anti-mouse IgG antibody, concentration is 2mg/ml, rule on nitrocellulose membrane, this line is parallel with infections chicken cloacal bursa virus resisting detection line 7, and line-to-line is every 5mm, should be careful even, room temperature was dried 20 minutes.The liquid of this three-way usefulness infiltrates respectively in the nitrocellulose membrane, promptly is respectively anti-newcastle disease virus detection line 6, infections chicken cloacal bursa virus resisting detection line 7 and control line 8.
Coated film 4 prepares: the nitrocellulose membrane that will contain detection line 6 and control line 8 with above-mentioned sealing working fluid takes out to dry under rearmounted 37 ℃ and handled two hours in 37 ℃ of sealings 60 minutes, and envelope is standby.
The preparation of the glass fibre membrane 3 of coated with gold labeling antibody:
The preparation of aqueous solution of chloraurate: the 10g gold chloride with the dissolving of 1000ml distilled water, is made into the aqueous solution of 1 ﹪, place 4 ℃ standby, the term of validity is 3 days.
The configuration of trisodium citrate: dissolve trisodium citrate with distilled water, be made into 1% aqueous solution, place 4 ℃ standby, the term of validity is 3 days.
0.1M the preparation of wet chemical: 13.8g sal tartari is mixed with the 0.1M wet chemical with 1000ml with distilled water, with 0.22u membrane filtration mistake, place 4 ℃ standby, the term of validity is 7 days.
The preparation of mark cleansing solution: 20g BSA is configured to 2%BSA solution with the PBS of 1000ml 0.01M, pH7.0, with 0.22u membrane filtration mistake, place 4 ℃ standby, the term of validity is 15 days.
The configuration that the gold labeling antibody is preserved liquid: with 10g BSA, 5g skimmed milk power, 0.5g NaN
3Dissolve in the PBS of 1000 ml 0.01M, pH 7.0 with 1ml Tween-20, with 0.22u membrane filtration mistake, place 4 ℃ standby, the term of validity is 15 days.
Firing of collaurum: 1% gold chloride is diluted to 0.01% with distilled water, put electric furnace and boil, add 4 milliliter of 1% citrate three sodium, continue to boil by per 100 milliliter of 0.01% gold chloride, be shiny red up to liquid and promptly stop heating, supply dehydration after being cooled to room temperature.Outward appearance should be pure, and is bright, do not have precipitation and floating thing.
The preparation of gold labeling antibody: transfer collaurum pH value to 7.6 with the 0.1M wet chemical, add the monoclonal antibody of anti-ewcastle disease and IBV by 20 micrograms antibody/milliliter collaurum, mixing left standstill 30 minutes, with 12000rpm centrifugal 30 minutes, abandoning supernatant, precipitation mark cleansing solution washed twice, last abandoning supernatant will precipitate with the golden labeling antibody of 1/10th initial collaurum volumes and preserve the liquid dissolving, place 4 ℃ standby, the term of validity is 7 days.
The collaurum that mark is good is layered on the glass fibre membrane equably, with 10 square centimeters of every ml soln shops, drying, envelope, place 4 ℃ standby.
The making of colloidal gold strip
End liner 1, sample pad 2 and thieving paper 5 are the general parts in this area.Above-mentioned coated film 4, the glass fibre membrane 3 that covers golden labeling antibody, end liner 1, sample pad 2 and thieving paper 5 are pasted successively, obtained test paper plate, the test strips that at last this test paper plate is cut into different in width gets final product.
The application of above-mentioned colloidal gold strip in detecting newcastle disease and infectiousness bursal disease poison
This test strips can be used for detecting the fluid sample that contains in the oral cavity of period of disease chicken or the anal swab, during operation, centrifugal in oral cavity of period of disease chicken or the anal swab is separated, the fluid drips of gained is added on the sample pad 2 of this test strips, when the result who occurs an aubergine band respectively occurred on the detection of antibodies line 7 of the detection of antibodies line 6 of the newcastle disease virus of test strips, avian infectious bursal disease poison and control line 8 positions, newcastle disease and infectiousness bursal disease poison were positive findings; When on the detection of antibodies line 6 of the newcastle disease virus of test strips and control line 8 positions, an aubergine band respectively occurring, the positive result of newcastle disease virus; When on the detection of antibodies line 7 of the avian infectious bursal disease poison of test strips and control line 8 positions, an aubergine band respectively occurring, the positive result of avian infectious bursal disease poison; When only an aubergine band occurring on control line 8 positions of test strips, negative result; When the aubergine band not occurring on control line 8 positions of test strips is null result.
The above, it only is preferred embodiment of the present utility model, be not to be the restriction of the utility model being made other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the equivalent embodiment of equivalent variations.But every technical solutions of the utility model content that do not break away to any simple modification, equivalent variations and remodeling that above embodiment did, still belongs to the protection domain of technical solutions of the utility model according to technical spirit of the present utility model.
Claims (4)
1. one kind one goes on foot the test strips that detects newcastle disease and infectiousness bursal disease poison, it is characterized in that comprising end liner, obedient coated film is glued at middle part above the described end liner, described coated film middle part have a bag by the detection of antibodies line of newcastle disease virus, bag by the detection of antibodies line of avian infectious bursal disease poison and a bag by the control line of anti-mouse IgG antibody, the glass fibre membrane and the thieving paper of sticking obedient coated with gold labeling antibody respectively about described coated film top are pasted sample pad above the described glass fibre membrane.
2. test strips according to claim 1 is characterized in that: described bag is monoclonal antibody or polyclonal antibody by the antibody of newcastle disease virus.
3. test strips according to claim 1 is characterized in that: described bag is monoclonal antibody or polyclonal antibody by the antibody of avian infectious bursal disease poison.
4. test strips according to claim 1 is characterized in that: the monoclonal antibody by the anti-newcastle disease virus and the anti-avian infectious bursal disease poison of colloid gold particle mark is arranged in the glass fibre membrane of described coated with gold labeling antibody.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020206457 CN201859147U (en) | 2010-05-28 | 2010-05-28 | Test strip for detecting Newcastle disease and infectious bursal disease virus in one step |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020206457 CN201859147U (en) | 2010-05-28 | 2010-05-28 | Test strip for detecting Newcastle disease and infectious bursal disease virus in one step |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201859147U true CN201859147U (en) | 2011-06-08 |
Family
ID=44104951
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201020206457 Expired - Fee Related CN201859147U (en) | 2010-05-28 | 2010-05-28 | Test strip for detecting Newcastle disease and infectious bursal disease virus in one step |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201859147U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103235129A (en) * | 2013-04-17 | 2013-08-07 | 河南省农业科学院 | Marek's disease virus and subgroup-J avian leukosis virus rapid combined-detection test strip |
| CN104614530A (en) * | 2014-11-20 | 2015-05-13 | 江苏省原子医学研究所 | Test strip for detecting pepsinogen I and pepsinogen II as well as detection method and application of test strip |
-
2010
- 2010-05-28 CN CN 201020206457 patent/CN201859147U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103235129A (en) * | 2013-04-17 | 2013-08-07 | 河南省农业科学院 | Marek's disease virus and subgroup-J avian leukosis virus rapid combined-detection test strip |
| CN103235129B (en) * | 2013-04-17 | 2015-10-28 | 河南省农业科学院 | Marek's disease virus and J subgroup avian leucosis virus fast joint inspection test strips |
| CN104614530A (en) * | 2014-11-20 | 2015-05-13 | 江苏省原子医学研究所 | Test strip for detecting pepsinogen I and pepsinogen II as well as detection method and application of test strip |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101930006B (en) | High-sensitivity immunochromatographic test strip for detecting total aflatoxin content quickly and preparation method thereof | |
| CN104007261B (en) | Fowl three kinds of breathing problem three quick detection kit and application | |
| CN107942061A (en) | A kind of test card, preparation and its detection method for detecting transmissible gastro-enteritis virus antibody | |
| CN102747040B (en) | Anti-influenza A virus nucleoprotein monoclonal antibody, its preparation and application | |
| CN103048459B (en) | Immune detection reagent for detecting respiratory syncytial virus | |
| CN103266088B (en) | A kind of H7 subtype avian influenza virus monoclonal antibody and test kit | |
| CN102967710A (en) | Competitive ELISA kit for peste-des-petits-ruminants antibody detection and preparation method thereof | |
| CN104181298B (en) | A kind of human parainfluenza virus's IgM antibody test strip and preparation method thereof | |
| CN101614737A (en) | A kind of colloidal gold strip and its production and application | |
| CN104109206A (en) | Monoclonal antibodies against duck Tembusu virus, antigen detection kit and application | |
| CN106405093A (en) | Detection kit for detecting infectious bovine rhinotracheitis virus antibody | |
| CN101403759A (en) | ELISA detection method and reagent kit for bluetongue viral antigen | |
| CN101348777B (en) | Influenza A virus ELISA nucleoprotein capture antigen diagnose reagent kit and special monoclonal antibody therefor | |
| CN104569408A (en) | Fenoterol colloidal gold detection card and preparation method thereof | |
| CN201859147U (en) | Test strip for detecting Newcastle disease and infectious bursal disease virus in one step | |
| CN102109526B (en) | Test paper for quickly detecting grass carp reovirus (GCRV) and preparation method and using method thereof | |
| CN101706499A (en) | FLAG fusion tag colloidal gold test strip and preparation method thereof | |
| CN103592436A (en) | A-type H1 subtype influenza virus antibody blocking ELISA kit and applications thereof | |
| CN101852802A (en) | Test paper for detecting newcastle disease and infectious bronchitis viruses in one step | |
| CN108918875A (en) | Pigeon with newcastle disease monoclonal antibody and the application in preparation diagnosis and detection kit | |
| CN105891469A (en) | Portunus trituberculatus reovirus detection test paper and preparation method thereof | |
| CN202599960U (en) | Colloidal gold test strip for rapid detection of O-type foot-and-mouth disease virus, classical swine fever virus and porcine reproductive and respiratory syndrome virus | |
| CN102633878A (en) | H1-subtype influenza A virus double-antibody sandwich ELISA kit and application | |
| CN101017173A (en) | Method for detecting equine coronavirus antibody and special diagnosis kit thereof | |
| CN201413328Y (en) | Colloidal gold test paper strip for rapidly testing swine flu H1N1 virus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Weifang Noted Pharmaceutical Co., Ltd. Assignor: Qingdao continet Pharmaceutical Co. Ltd.|Qingdao boite biopharmaceutical Co. Ltd. Contract record no.: 2011370000087 Denomination of utility model: Test strip for detecting Newcastle disease and infectious bursal disease virus in one step Granted publication date: 20110608 License type: Exclusive License Record date: 20110412 |
|
| C56 | Change in the name or address of the patentee |
Owner name: QINGDAO KDN PHARMACEUTICAL CO., LTD. Free format text: FORMER NAME: QINGDAO CONTINENT BIOTECH CO., LTD. |
|
| CP01 | Change in the name or title of a patent holder |
Address after: 266061 Shandong city of Qingdao province high tech park of Laoshan district by the No. 29 Shandong Road, building 605 high speed Co-patentee after: Qingdao Boite Biopharmaceutical Co., Ltd. Patentee after: Qingdao KDN Pharmaceutical Co., Ltd. Address before: 266061 Shandong city of Qingdao province high tech park of Laoshan district by the No. 29 Shandong Road, building 605 high speed Co-patentee before: Qingdao Boite Biopharmaceutical Co., Ltd. Patentee before: Qingdao Continent Biotech Co., Ltd. |
|
| C56 | Change in the name or address of the patentee |
Owner name: QINGDAO WEILAN BIOLOGY CO., LTD. Free format text: FORMER NAME: QINGDAO KDN PHARMACEUTICAL CO., LTD. |
|
| CP03 | Change of name, title or address |
Address after: 266111 Shandong city of Qingdao province Chengyang Qingda Industrial Park, the first northbound dual Patentee after: QINGDAO VLAND BIOLOGICAL CO., LTD. Patentee after: Qingdao Boite Biopharmaceutical Co., Ltd. Address before: 266061 Shandong city of Qingdao province high tech park of Laoshan district by the No. 29 Shandong Road, building 605 high speed Patentee before: Qingdao KDN Pharmaceutical Co., Ltd. Patentee before: Qingdao Boite Biopharmaceutical Co., Ltd. |
|
| CP03 | Change of name, title or address | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 266111 Shandong city of Qingdao province Chengyang Qingda Industrial Park, the first northbound dual Patentee after: QINGDAO VLAND BIOLOGICAL CO., LTD. Patentee after: QINGDAO VLAND BIOLOGICAL PRODUCTS CO., LTD. Address before: 266111 Shandong city of Qingdao province Chengyang Qingda Industrial Park, the first northbound dual Patentee before: QINGDAO VLAND BIOLOGICAL CO., LTD. Patentee before: Qingdao Boite Biopharmaceutical Co., Ltd. |
|
| CP01 | Change in the name or title of a patent holder | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110608 Termination date: 20190528 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |